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불확실한 환경에서 조립을 수행하는 두 대의 로봇 팔 제어
정성엽,강경대,이두용,Jeong, Seong-Yeop,Gang, Gyeong-Dae,Lee, Du-Yong 대한기계학회 2000 大韓機械學會論文集A Vol.24 No.12
Assembly tasks are often performed by one robot with fixtures. This type of assembly system has low flexibility in terms of the variety of parts and the part-presentation the system can handle. This paper addresses assembly without fixtures using two-manipulator robot. An active method using force feedback is proposed for the peg-in-hole assembly in highly uncertain environment. Assembly states are defined as status having unique motion constraints and events are modeled as variation of the environmental force. The states are recognized through identification of the events using two 6-d. o. f. force/moment sensors. The proposed method is verified and evaluated by experiments with round peg-in-hole assembly.
<sup>110m</sup>Ag 간섭으로부터 <sup>137</sup>Cs 정량평가를 위한 드럼핵종분석 교정기술
정성엽,Jeong, Sung Yeop 한국방사성폐기물학회 2016 방사성폐기물학회지 Vol.14 No.1
The Tomographic Gamma Scan (TGS) technique partitions radioactive waste drums into $10{\times}10{\times}16$ voxels and assays both the density and concentration of radioactivity for each voxel thus providing for improved accuracy, when compared to the traditional Non-Destructive Assay(NDA) techniques. It could decrease the degree of precision measurement since there is a trade-off between spatial resolution and precision. This latter drawback is compensated by expanding the Region of Interest (ROI) that differentiates the full energy peaks, which, in turn, results in an optimized degree of precision. The enlarged ROI, however, increases the probability of interference among those nuclides that emit energies in the adjacent spectrum. This study has identified the cause of such interference for the reference nuclide of the TGS technique, $^{137}Cs$ (661.66 keV, half-life 30.5 years), to be $^{110m}Ag$ (657.75 keV, half-life 249.76 days). A new calibration method of determining the optimized ROI was developed, and its effectiveness in accurately characterizing $^{137}Cs$ and eliminating the interference was further ascertained.
열적가용화공정을 이용한 하수슬러지의 가용화 특성 및 바이오가스 생산량 평가
정성엽,연호석,이창열,이종인,장순웅,Jeong, Seong-Yeob,Yeon, Ho-Suk,Lee, Chang-Yeol,Lee, Jong-In,Chang, Soon-Woong 한국자원리싸이클링학회 2015 資源 리싸이클링 Vol.24 No.2
본 연구는 하수처리장에서 발생되는 생슬러지와 잉여슬러지를 대상으로 열적가용화 공정의 적용성을 평가하였다. 열적가용화 효율 및 특성평가는 각 슬러지를 대상으로 $100{\sim}220^{\circ}C$ 온도범위에서 30분간 전처리를 수행한 후 실시되었다. 그 결과, 가용화 온도가 상승함에 따라 $SCOD_{Cr}$, $NH_4{^+}$, VFAs 농도가 증가하는 경향을 확인할 수 있었다. 또한 COD 가용화효율 평가 결과, 온도 상승에 비례하는 경향이 나타나 열적가용화에 의해 가수분해 및 산발표 단계를 촉진시킨 것으로 판단된다. BMP (Biochemical Methane Potential) 실험을 통해 생슬러지의 경우 $220^{\circ}C$에서 가장 높은 biogas 생산량을 보여주었으나, 증가율은 5.6%로 열적가용화에 의한 효과가 미미한 것으로 나타났다. 반면 잉여슬러지의 경우 최대 38.8% ( $180^{\circ}C$) 증가하여, 열적가용화 공정은 잉여슬러지에 대한 적용성이 더욱 우수한 것으로 나타났다. In this study, an applicability of thermal pretreatment for primary and secondary sludge, which are generated in a sewage treatment plant, was evaluated. The efficiency and charateristics was investigated with each sludge after pretreatment under the condition of $100{\sim}220^{\circ}C$ for 30 minutes. As the result, it was found that $SCOD_{Cr}$, $NH_4{^+}$, VFAs concentrations increased as the pre-treatment temperature increased. For COD solubilization, it was also highly dependent on an increase of temperature resulting in acceleration on hydrolysis and acid fermentation. In the BMP (Biochemical Methane Potential) experiment, for the primary sludge, it showed the higher biogas production rate at a temperature of $220^{\circ}C$, however, the effect was insignificant (5.6%). Whereas, for the secondary sludge, the increase on biogass production rate was 38.8% ($180^{\circ}C$) and this means that the secondary sludge is more suitable for an applicability of thermal pretreatment.
김치에서 분리한 Lactococcus lactis ET45가 생산하는 박테리오신의 특성
정성엽,박찬선,최낙식,양희종,김차영,윤병대,강대욱,유연우,김민수,Jeong, Seong-Yeop,Park, Chan-Sun,Choi, Nack-Shick,Yang, Hee-Jong,Kim, Cha-Young,Yoon, Byoung-Dae,Kang, Dae-Ook,Ryu, Yeon-Woo,Kim, Min-Soo 한국미생물학회 2011 미생물학회지 Vol.47 No.1
Bacillus cereus에 항균활성을 갖는 박테리오신을 생산하는 유산균을 김치로부터 분리하였다. 선별한 균주는 Bergey's manual, 16S rDNA 분석을 통하여 Lactococcus lactis로 동정되었으며, L. lactis ET45로 명명하였다. ET45가 생산하는 박테리오신은 pH 3.0-11.0까지 안정하였고, 열 안정성 테스트결과 $40-121^{\circ}C$까지 안정함을 확인하였다. 박테리오신의 생산을 위한 최적 조건으로 MRS 액체배지에서 초기 pH 7.5, $30^{\circ}C$에서 18시간 배양하였을 때 최대생산을 보였다. 박테리오신의 항균활성이 proteinase K의 처리로 활성이 소실되어, 박테리오신이 단백질성 물질임을 확인하였다. 박테리오신의 분자량은 tricine sodium dodecyl sulfate polyacryamide gel electrophoresis (TSDS-PAGE)를 통하여 약 4.5 kDa임을 확인하였다. Bacteriocin-producing lactic acid bacterium having antagonistic activity against Bacillus cereus, was isolated from Kimchi. The selected strain was identified as Lactococcus lactis by the Bergey's manual and 16S rDNA analysis, and named as L. lactis ET45. The bacteriocin was stable in the pH range 3.0-11.0. The bacteriocin was active over a wide temperature range from $40^{\circ}C$ to $121^{\circ}C$. Optimal culture condition for producing bacteriocin was obtained by growing the cells on MRS medium at pH 7.5 and $30^{\circ}C$ for 18 h. Antibacterial activity of the bacteriocin was completely disappeared by proteinase K, and this means that bacteriocin is a proteinous substance. The molecular weight of bacteriocin was estimated to be about 4.5 kDa by tricine sodium dodecyl sulfate polyacryamide gel electrophoresis (TSDS-PAGE).
기전력법을 이용한 용융 Zn-Cd 합금중 Zn의 활동도 측정
정성엽,정우광,박종진,Jeong, Seong-Yeop,Jeong, U-Gwang,Park, Jong-Jin 한국재료학회 2002 한국재료학회지 Vol.12 No.4
The E.M.F of the galvanic cell with fused salt was measured to determine the activities of zinc at 700-820K over the entire composition range of liquid Zn-Cd alloys. The cell used was as follows: (-) W | Zn(pure) $Zn^{2+}(KCI-LiCl)$ | Zn(in Zn-Cd alloy) | W (+) The activities of zinc in the alloys showed positive deviation from Raoult's law over the entire composition range. The activity of cadmium and some thermodynamic functions such as Gibbs free energy, enthalpy, entropy were derived from the results by the thermodynamic relationship. The comparison of the results and the literature data was made. The liquid Zn-Cd alloy is found to be close to the regular solution. The concentration fluctuations in long wavelength limit, $S_{cc}(o)$, in the liquid alloy was calculated from the results.
호상 요구르트에 적합한 Lactobacillus plantarum strains 탐색 및 요구르트의 이화학적 및 관능 특성
정성엽(Seong-Yeop Jeong),이용현(Yong Hyen Lee),강선아(Suna Kang),신배근(Bae Keun Shin),박선민(Sunmin Park) 한국식품과학회 2016 한국식품과학회지 Vol.48 No.6
본 연구에서는 커드가 잘 생성 되는 김치 젖산세균을 탐색 및 동정을 통한 L. plantarum YD2, YD9, YD12을 분리하였으며, 분리한 젖산세균을 이용한 요구르트를 제조하여 이화학적인 특성을 알아보았다. pH와 산도의 변화는 발효시간에 따라 크게 달라졌으며, 12시간 이후 pH는 감소하는 반면 산도는 증가하였다. 점성의 경우 젖산세균의 pH와 산도에 따라 값의 변화가 있었고, pH가 낮고 산도가 높은 경우, 시간에 지남에 따라 점성 값이 감소하는 경향을 보았다. 이는 생성된 유기산이 단백질의 변성을 일으켜 요구르트 내 겔의 형성을 방해하여 점성을 떨어뜨리는 것으로 사료된다. 요구르트의 이화학적 실험 결과에 있어서 김치에서 얻어낸 젖산세균인 L. plantarum에서 커드를 형성하는 균주를 이용하여 요구르트를 제조하였을 때, 커드의 형성이 잘 이루어졌으므로, 요구르트 제조에 있어서 L. plantarum 젖산세균이 적합한 것으로 사료된다. 관능검사 결과, 대조군을 제외한 실험군 중 YD12군이 전체적인 기호도에서 가장 높은 값을 얻었으나 대조군에 비하여 낮은 값을 나타내었다. YD12군을 이용해 요구르트를 제조할 때 풍미와 신맛, 쓴맛을 보완시킨다면 시판되는 요구르트와 유사한 혹은 더 높은 기호도를 얻을 수 있을 것으로 기대한다. 김치 젖산세균을 이용한 요구르트 제조 시, 김치 젖산세균이 위산에 대해 내성을 가지고 있으므로 젖산세균이 장까지 잘 전달이 될 것 이라 기대한다. Since some strains of kimchi lactobacilli can curdle milk, they can be used for making yogurt. However, the best Lactobacillus plantarum strains for curdling milk for yogurt are still unknown. In this study, we determined the best L. plantarum strains for curdling milk, and the physicochemical properties of yogurts made using different L. plantarum strains were examined. Three strains of L. plantarum useful for curdling milk were identified (YD2, YD9, YD12). The number of lactobacilli was lower in yogurts made with L. plantarum than in those made with control, and among the L. plantarum strains tested, YD12 had the highest bacterial counts. However, the microbial count reached 6.3×10<SUP>8</SUP> CFU/mL after 24-h fermentation in all yogurts. The pH of the yogurts decreased after 12-h fermentation, while the acidity increased. The low pH and high acidity decreased the viscosity in all the three types of yogurts, because the acids disturbed gel formation due to protein denaturation. Sensory evaluation revealed that the YD12 group showed a high percentage of completion similar to the control group. YD2 and YD9 showed a high sourness value and low sweetness alue, whereas YD12 yielded optimal values for all the organoleptic characteristics. Therefore, YD12 would be a high quality bacterial strain for use as a yogurt starter culture.
정성엽(Seong-yeob Jeong),임수민(Soo-min Lim),김영신(Youngshin Kim) 한국교원대학교 뇌기반교육연구소 2019 Brain, Digital, & Learning Vol.9 No.3
Recent scientific inquiries emphasize the process by which students make their own observations and find answers. In order to facilitate the inquiry activities, students should use the right scientific concept. The purpose to this study are to analyze the coherence of scientific concept and scientific concept used in the observation, question generation and experimental design stage for 12 elementary and middle school students. To do this, a think-aloud method and a semantic network analysis were used. The conclusions are as follows. First, concepts that are of interest continue to be used in the scientific inquiry stage. Most of these concepts are related to experimental instruments. Second, the concepts of high frequency are representative of the concept network of each process skills. Further researches, it is needed to be done on the coherence of the scientific concept that students use at other stages.