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입자사출기법에 (粒子射出技法) 의한 벼 세포로의 외래 유전자 도입과 그 발현
전종성(Jong Seong Jeon),정호성(Hou Sung Jung),성순기(Soon Kee Sung),이종섭(Jong Seob Lee),최양도(Yang Do Choi),김한집(Han Jip Kim),이광웅(Kwang Woong Lee) 한국식물학회 1994 Journal of Plant Biology Vol.37 No.1
For establishing a transformation system of rice, an efficient introduction of foreign genes into embryogenic cell suspension by particle bombardment was conducted. The particle inflow gun based on the acceleration of DNA-coated tungsten particles using pressurized helium was constructed for delivery of DNA into rice cells. Several bombardment parameters were optimized using the transient expression of GUS gene. The conditions that gave the highests GUS gene expression of about 1000 blue spots per a g fresh weight of bombarded cells include treatment of the cells with 0.5 M osmotic pressure, and use of the 410 kPa helium, 100 mm target distance, 13 mm syringe filter holder and 5 μL DNA/tungsten mixtures. It was also confirmed that rice actin promoter-intron constract gave the highest expression of all promoter-sequences studied. Eight weeks after the bombardment, stably transformed calluses were obtained on the selection medium containing 100 mg/L G418 and showed the strong activity in in situ GUS assay.
형질전환된 벼에서 CaMV 35S 프로모터 및 벼 Act1 프로모터에 의한 GUS 의 발현
전종성(Jong Seong Jeon),김정훈(Jung Hoon Kim),최동수(Dong Su Choi),이종섭(Jong Seob Lee),최양도(Yang Do Choi),이광웅(Kwang Woong Lee) 한국식물학회 1994 Journal of Plant Biology Vol.37 No.3
To determine the patterns and the levels of expression of the cauliflower mosaic virus (CaMV 35S) promoter and the rice actin 1 (Act1) promoter in rice, transgenic rice plants containing CaMV 35S-β-glucuronidase (GUS) and Act1-GUS constructs were generated and examined by fluorometric and histochemical analyses. The fluorometric analysis of stably transformed calluses showed that the activity of the rice Act1 promoter was stronger than that of the CaMV 35S promoter in rice cells. In a histochemical study of the transgenic rices, it was shown that the GUS activity directed by tne CaMV 35S promoter was localized mainly in parenchymal cells of vascular tissues of leaves and roots and mesophyll cells of leaves. These results are similar to those of potato, a dicot plant. In contrast, rice plant transformed with Act1-GUS fusion construct revealed strong GUS activity in parenchymal cells of vascular tissue, mesophyll cells, epidermal cells, bulliform cells, guard and subsidiary cells of leaves and most cells of the root, suggesting that the rice Act1 promoter is more constitutive than the CaMV 35S promoter. It was also confirmed that in both types of transgenic rice little or no staining was localized in metaxylem tracheary elements of vascular tissue from leaves or roots. These results indicate that the rice Act1 promoter can be utilized more successfully for expression of a variety of foreign genes in rice than the CaMV 35S promoter.
류학승,류나연,정기홍,안진흥,전종성,Ryu, Hak-Seung,Ryoo, Na-Yeon,Jung, Ki-Hong,An, Gynheung,Jeon, Jong-Seong 한국식물생명공학회 2010 식물생명공학회지 Vol.37 No.2
Rice (Oryza sativa) is a major cereal crop that has been developed as a monocot model species. In past decades rice researchers have established valuable resources for functional genomics in rice, such as complete genome sequencing, high-density genetic maps, a full length cDNA database, genome-wide transcriptome data, and a large number of mutants. Of these, rice mutant lines are very important to definitively determine functions of genes associated with valuable agronomic traits. In this review we summarize the progress of functional genomics approaches in rice using T-DNA mutants.
김영수(Young Soo Kim),전종성(Jong Seong Jeon),이광웅(Kwang Woong Lee) 한국식물학회 1993 Journal of Plant Biology Vol.36 No.2
Efficient plant regeneration system was established through the culture of rice (Oryza sativa L.) microspores. Microspores released by anther shedding culture developed into proembryos and calluses in B5 medium within two weeks of culture. The optimal hormone and carbon sources were dependent on the genotypes used. Microspore`s viability decreased rapidly in culture time, therefore less than 3% of the total microspores were viable at the 9th day when the first microspore division was observed. Of two types of microspores (pollen dimorphism) observed in culture, only the P-grain, larger microspoers than normal ones was able to divide. Using 4`,6-diamidino-2-phenylindole (DAPI) fluorescence staining, it was confirmed that the symmetrical division of uninucleate microspore was the first step leading to continuous microspore development. Microspore-derived proembryos and calluses were regenerated to plants in N6 medium containing 1 mg/L NAA and 5 mg/L kinetin, and 78.3% of the regenerated plants were haploids.
형질전환된 감자의 후대 식물체에서 CaMV 35S Promoter - GUS 유전자 발현
성순기(Soon Kee Sung),최상봉(Sang Bong Choi),전종성(Jong Seong Jeon),박민철(Min Chul Park),이광웅(Kwang Woong Lee) 한국식물학회 1994 Journal of Plant Biology Vol.37 No.1
Two potato (Solanum tuberosum L.) cultivars were transformed by Agrobacterium tumefaciens harboring cauliflower mosaic virus (CaMV) 35S promoter and β-glucuronidase (GUS) gene. Expression patterns of the CaMV 35S promoter according to tissue types and deveplopmental stages, and genetic stability of GUS gene were investigated in the clonal progenies of transgenic potatoes. Kanamycin-resistant shoot emerged from tuber disc after 4 weeks of culture, and root was induced 6 weeks after culture on the selection medium. Shooting frequency of cvs. Superior and Dejima were 43% and 27%, respectively. Mature transformants and their clonal progenies showed no phenotypical abnormality. GUS activity was expressed primarily at parenchymatous cells of phloem tissue around the vascular cambium in the stem and root, and higher activity was found at the apical meristem of shoot, root and adventious shoot bud. GUS activity was higher at tubers of young explants than at stored tubers. These facts indicate that expression level of the CaMV 35S promoter differred according to tissue types and developmental stages of the organs. The GUS gene was stably inherited to each clonal progeny and normally expressed.
생화학,분자생물학 : DEB 처리에 의해 유도된 벼 돌연변이 집단으로부터 도열병 감수성 돌연변이 분리
김혜경 ( Hye Kyung Kim ),이상규 ( Sang Kyu Lee ),한무호 ( Mu Ho Han ),전용희 ( Yong Hee Jeon ),이기환 ( Gi Hwan Yi ),이윤형 ( Youn Hyung Lee ),부성희 ( Seong Hee Bhoo ),한태룡 ( Tae Ryong Hahn ),전종성 ( Jong Seong Jeon ) 한국응용생명화학회 2005 Applied Biological Chemistry (Appl Biol Chem) Vol.48 No.4