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임장수,전인동,이유빈,김은미,신동석,전호정,박한수,신흥수 한국고분자학회 2016 Macromolecular Research Vol.24 No.6
Although many efforts have been made to engineer cell sheets with anisotropic patterns, current techniques still exhibit several shortcomings including uncontrolled harvest time and deformation of pattern by contraction. In this study, we report a simple method to harvest a cell sheet with a striated structure of extracellular matrix (ECM) and myoblasts using a thermosensitive hydrogel micropatterned with different sizes (25 and 80 μm). The hydrogel supported the formation of a confluent monolayer of myoblasts with aligned morphology. When the temperature was reduced from 37 to 4 oC, the size of the hydrogel increased by approximately 1.2-fold within 10 min. In response to this change, a cell sheet was harvested and could be transferred to the desirable substrate through conformal contact between the hydrogel and target. We further examined the effect of pattern size on alignment of ECM/ cell assembly by fast Fourier transform (FFT) analysis of fluorescently stained stress fibers and ECM proteins within the harvested cell sheet. The cell sheet maintained alignment of confluent myoblasts after being harvested to the substrate. In addition, the topologic patterns also promoted formation of aligned myotube on the harvested cell sheet, which was important for muscle tissue regeneration. However, the pattern size appeared to have no influence on alignment of cells on the cell sheet. Finally, a bi-layered structure was fabricated in which each layer was stacked with aligned direction on the cell sheet being perpendicularly assembled. Collectively, our platform could be used for rapid harvest of cell sheets with aligned architecture of ECM/cell, which can be applied to fabrication of welldefined 3D tissue for tissue engineering.
임장수,이양민,이재기 동아대학교 정보기술연구소 2003 情報技術硏究所論文誌 Vol.10 No.2
Variety of Collaborative Virtual Environment systems were developed until now and many researches are under the progress at next generation Internet. In this paper, we propose a Virtual Collaborative Performance System design by wireless control that is a type of CVE. Previous Collaborative system can not satisfy special condition of a performance system. The systems are need to be synchronized but it is work hard. The system which we propose is satisfied with the synchronization among players.