식물성 질소원의 보충이 면양에 의한 볏짚의 소화율에 미치는 영향

이희섭,강희신 ( Hee Seob Lee,Hi Shin Kang ) 한국축산학회 1980 한국축산학회지 Vol.22 No.2

Three Corridale male sheep were fed each of the three diets; Acacia leaf hay alone (AL), chopped rice st raw plus near isonitrogeneous quantity of Soybean oil meal (RS) or Acacia Leaf Hay(RA) according to a Latin square design to studs the effect of supplemental nitrogen on the digestibilities of nutrients and the voluntary intake of rice straw. The results obtained from the above trial were summarized as follows: (1) The voluntary feed intake of acacia leaf hay was found to he 935g/day and its digestibility of dry matter, crude protein and crude fibre were 51.8, 46.0 and 52.0% respectively. (2) Although the total UM intake were not affected by the supplemental nitrogen sources, the intake of chopped rice straw by the sheep at the RS was 11.7% higher thar that of RA. (3) The crude protein intake of sheep at the AL was higher (p. 05) than those of RS and RA. The crude protein digestibility of RS, however, was higher than those of AL and RA as much as 71.7% and 64.2%. (4) Despite the trend that the liber intake of sheep fed chopped rice straw, RS and RA, were higher than that of AL as much as 49.3% and 64.3% the digestibility of crude fiber showed no significant difference among the treatments. The difference between the RS and AL., however, was significant at level of 10% probability. The total digestible nutrients of chopped rice straw was calculated to be 50% in DM basis by employing the TDN value of known Soybean oil meal and Acacia leaf hay.

Effects of an Aqueous Extract of Asparagus cochinchinensis on the Regulation of Nerve Growth Factor in Neuronal Cells

Hyun Ah Lee(이현아),Ji Eun Kim(김지은),Sung Hwa Song(송성화),Ji Eun Sung(성지은),Min Gi Jung(정민기),Dong Seob Kim(김동섭),Hong Joo Son(손홍주),Chung Yeoul Lee(이충열),Hee Seob Lee(이희섭),Dae Youn Hwang(황대연) 한국생명과학회 2016 생명과학회지 Vol.26 No.5

천문동(Asparagus cochinchinensis)은 북아시아 지역에서 열병, 감기, 신장질환, 유방암, 염증질환, 뇌질환 등의 치료에 오랫동안 사용되어온 약용식물(medicinal plant)이다. 비록, 천문동의 항염증(ani-inflammatory) 효능에 대한 일부 연구들이 수행되었지만, 신경세포에서 항염증작용과 신경성장인자(nerve growth factor, NGF)의 연관성에 대한 연구는 수행된바 없다. 따라서, 본 연구에서는 신경세포에서 신경성장인자의 분비와 작용기전에 대한 천문동 열수추출물(aqueous extract from A. cochinchinensis, AEAC)의 영향을 연구하였다. AEAC로 처리된 B35세포의 배양액에 NGF단백질의 농도는 대조물질(vehicle) 처리군에 비하여 유의적으로 증가하였으며, 특별한 독성은 관찰되지 않았다. 또한, NGF mRNA의 발현도 단백질의 농도변화와 유사한 양상을 나타내었다. 더불어, B35세포로부터 분비된 NGF의 생리활성을 확인하기 위해, AEAC-조정배지(conditioned medium)를 미분화된 PC12세포에 처리한 후 이들 세포의 신경염성 성장(neuritic outgrowth)을 관찰하였다. PC12세포의 수상돌기 길이(dendritic length)는 vehicle처리군에 비하여 AEAC-조정배지처리군에서 유의적으로 증가하였다. 또한, High affinity NGF 수용체의 하위신호전달에 포함된 p-TrkA와 p-ERK의 발현은 AEAC-조정배지처리군에서 높았지만, low affinity NGF 수용체의 하위신호전달에서는 낮은 수준으로 관찰되었다. 이러한 결과는 AEAC가 신경세포에서 NGF발현과 분비의 조절에 기여하기 때문에 신경퇴행성질환(neurodegenerative disease) 치료제로서 우수한 후보물질임을 제시하고 있다. Asparagus cochinchinensis is a medical plant that has long been used to treat fever, cough, kidney disease, breast cancer, inflammatory disease and brain disease in northeast Asian countries. Although several studies have been conducted on the anti-neuroinflammatory effects of A. cochinchinensis, the correlation between these effects and nerve growth factor (NGF) has not yet been examined. In this study, we investigated the effects of an aqueous extract of A. cochinchinensis (AEAC) on the secretion and action mechanism of NGF in neuronal cells. The concentration of the NGF protein in the supernatant collected from cultured cells increased significantly in B35 cells treated with AEAC in comparison with the vehicle-treated group without any specific cytotoxicity. Furthermore, the mRNA expression of NGF showed a very similar pattern to its protein concentration. To examine the bioactivity of NGF secreted from B35 cells, undifferentiated PC12 cells were cultured in an AEAC-conditioned medium and neuritic outgrowth was observed. The dendrite length of PC12 cells in the AEAC-treated group was significantly higher than that in the vehicle-treated group. Moreover, the level of the downstream effectors p-TrkA and p-ERK of the high-affinity NGF receptor was significantly higher in the AEAC-treated group, while the expression of the downstream effectors of the low-affinity NGF receptor was significantly lower in the same group. These results suggest that AEAC may contribute to the regulation of NGF expression and secretion in neuronal cells; it is therefore an excellent candidate for further investigation as a therapeutic drug for neurodegenerative diseases.

Inhibitory Effects of Asparagus cochinchinensis in LPS-Stimulated BV-2 Microglial Cells through Regulation of Neuroinflammatory Mediators, the MAP Kinase Pathway, and the Cell Cycle

Hyun Ah Lee(이현아),Ji Eun Kim(김지은),Jun Young Choi(최준영),Ji Eun Sung(성지은),Woo Bin Youn(윤우빈),Hong Joo Son(손홍주),Hee Seob Lee(이희섭),Hyun-Gu Kang(강현구),Dae Youn Hwang(황대연) 한국생명과학회 2020 생명과학회지 Vol.30 No.4

미세교세포(Microglial cells)에서 신경염증반응(neuroinflammatory responses)의 억제는 알츠하이머질환, 파킨슨질환, 헌팅턴질환과 같은 신경퇴행성질환(neurodegenerative diseases)을 치료하기 위한 주요 표적으로 고려되고 있다. 천문동(Asparagus cochinchinesis)은 열, 기침, 신장 질환, 유방암, 염증성질환 및 뇌질환을 치료하는 데 오랫동안 사용 되어온 전통 치료제(Traditional medicine)이다. 본 연구에서는 lipopolysaccharide (LPS)로 활성화된 BV-2 미세교세포에서 항염증효과가 있는 천문동 뿌리 열수추출물(Aqueous extract from A. cochinchinesis root, AEAC)의 신경보호 메커니즘을 연구하였다. 먼저, 어떤 유의적인 세포독성은 플라보노이드(flavonoid), 페놀(phenol), 사포닌(saponin)을 함유하는 AEAC를 4가지 농도로 처리된 BV-2세포에서 검출되지 않았다. 또한, nitric oxide (NO), cyclooxygenase-2 (COX-2) mRNA 및 inducible nitric oxide synthase (iNOS) mRNA 수준은 AEAC+LPS 처리군에서 비하여 21%정도 감소하였다. 전염증성 사이토카인(TNF-α과 IL-1β) 및 항염증성 사이토카인(IL-6와 IL-10)농도에 대한 유사한 감소는 비록 감소비율은 다르지만, Vehicle+LPS 처리군에 비해 AEAC+LPS 처리군에서 검출되었다. 더불어, LPS 처리 후 mitogen-activated protein (MAP) kinase의 인산화수준의 증가는 AEAC 전처리군에서 유의하게 회복되었고, 세포주기에서 G2/M의 억제(arrest)는 AEAC+LPS 처리군에서 개선되었다. 또한, LPS 처리로 유도된 ROS의 증가도 AEAC 전처리군에서 감소되었다. 따라서, 이러한 결과는 AEAC가 MAPK 신호전달 경로, 세포주기 및 ROS (reactive oxygen species) 생성의 조절을 통해 LPS 자극에 대한 항신경염증 활성을 유도함을 제시하고 있다. The suppression of neuroinflammatory responses in microglial cells can be considered a key target for improving the progression of neurodegenerative diseases such as Alzheimer’s disease (AD), Parkinson’s disease (PD), and Huntington’s disease (HD). Asparagus cochinchinensis has traditionally been used as a medicine to treat fever, cough, kidney disease, breast cancer, inflammatory diseases, and brain diseases. In this study, we investigated the neuroprotective mechanism of an aqueous extract from A. cochinchinensis root (AEAC), particularly its anti-inflammatory effects on lipopolysaccharide (LPS)-activated BV-2 microglial cells. BV-2 cells were treated with four different concentrations of AEAC. No significant toxicity was detected in BV-2 cells treated with AEAC. Nitric oxide (NO), cyclooxygenase-2 (COX-2) mRNA, and inducible nitric oxide synthase (iNOS) mRNA levels were 21% lower in the AEAC+LPS group than in the Vehicle+LPS group. Lower proinflammatory (TNF-α and IL-1 β) and anti-inflammatory cytokine (IL-6 and IL-10) levels were also detected in the AEAC+LPS group than in the Vehicle+LPS group, albeit at varying rates. Moreover, the phosphorylation of mitogen-activated protein kinase (MAPK) members after LPS treatment was significantly recovered in the AEAC-pretreated group compared to the Vehicle+LPS group, enhancement of the phosphorylation of mitogen-activated protein kinase (MAPK) members after LPS treatment was significantly recovered in the AEAC-pretreated group, while cell cycle arrest at the G2/M phase caused by LPS treatment was less severe in the AEAC+LPS group. The increase in reactive oxygen species (ROS) generation induced by LPS treatment was also lower in the AEAC-pretreated group than in the Vehicle+LPS group. This is the first study to show that AEAC exerts anti-neuroinflammatory activity against LPS stimulation by regulating the MAPK signaling pathway, the cell cycle, and ROS production.

증숙 및 발효한 천문동의 항균활성과 특성

이승민 ( Seung-min Lee ),김수인 ( Su-in Kim ),강문선 ( Moon-sun Kang ),이충렬 ( Chung-yeol Lee ),황대연 ( Dae-youn Hwang ),이희섭 ( Hee-sup Lee ),김동섭 ( Dong-seob Kim ) 한국산업식품공학회 2017 산업 식품공학 Vol.21 No.2

본 연구에서는 증숙처리 후 발효를 통해 천문동 에틸아세테이트 추출물의 항균활성이 증가하는지 알아보고자 하였다. 선행연구에서 S. aureus와 P. aeruginosa에 대한 항균활성이 나타나지 않았던 L. plantarum KCCM 11322로 발효한 천문동의 경우 증숙 처리 후 발효를 하였을 때 25mg/mL 추출물 농도에서 생장 저해환이 나타났다. 증숙 후 발효한 천문동의 최소저해농도를 측정한 결과 선행연구에서 L. mesenteroides 4395로 발효한 천문동 추출물의 경우 최소저해농도가 25 mg/mL였으나 증숙 처리 후 발효시 10mg/mL인 것으로 나타났다. P. aeruginosa에 대한 최소저해농도는 증숙 처리 후 발효하였을 때 모든 추출물이 5 mg/mL인 것으로 나타났다. 이를 통해 천문동을 증숙 처리 후 발효를 하면 증숙 처리 하지 않고 발효한 천문동 보다 항균활성이 개선될 수 있을 것으로 사료되며 이는 증숙 처리를 통해 항균활성이 있는 것으로 보고된 폴리페놀과 플라보노이드의 함량이 증가하기 때문인 것으로 판단된다. 총당의 경우 증숙 처리시 감소되는 것으로 나타났고 환원당의 경우 증숙 처리를 통해 증가한 것으로 나타났다. 발효후 총당과 환원당은 모두 크게 감소하여 젖산균의 발효를 통해 당이 소비된 것으로 사료된다. 발효에 따른 pH 및 산도의 변화는 L. plantarum KCCM 11322로 발효하였을 때 L. mesenteroides 4395와 L. sakei 383로 발효한 것 보다 완만하게 변화하였으며 수분 및 회분은 발효균주에 따른 차이가 나타나지 않았다. This study was carried out to investigate antimicrobial activity and characteristics of Asparagus cochinchinenesis which was steamed and fermented with lactic acid bacteria. A. cochinchinensis was prepared to steaming process which was washed and freeze dried. A. cochinchinensis was steamed at 95℃ for 12 h and dried by hot air at 50oC for 24 h. After steaming process, A. cochinchinensis was fermented with lactic acid bacteria (Leuconostoc mesen-teroides 4395, Lactobacillus sakei 383 and Lactobacillus plantarum KCCM 11322). Ethyl acetate extracts of fermented A. cochinchinensis had antimicrobial activities for the respiratory disease bacteria (Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa and Escherichia coli). A. cochinchinensis had highest antimicrobial activity for the P. aeruginosa which fermented with L. mesenteroides 4395. The minimum inhibition concentration (MIC) of A. cochinchinensis fermented with L. mesenteroides 4395 was 10 mg/mL for S. aureus, S. epidermidis, E. coli and 5 mg/mL for P. aeruginosa. The MIC of A. cochinchinensis fermented with L. sakei 383 and A. cochinchinensis fermented with L. plantarum KCCM 11322 were the same. Total sugar was decreased from 863.33±17.47 mg/mL to 722.67±5.51 mg/mL during the steaming process. But reducing sugar was increased from 99.36±1.32 mg/mL to 109.29±2.71 mg/mL during the steaming process. Total sugar was decreased to 301.50-361.42 mg/mL and reducing sugar was decreased to 27.39-62.20 mg/mL during the fermentation process.

Protective Effect of Aqueous Extracts of Styela Clava Tunic Against Apoptosis of HepG2 Cells Induced by Hydrogen Peroxide

Eun Kyoung Koh(고은경),Young Ju Lee(이영주),Ji Eun Kim(김지은),Moon Hwa Kwak(곽문화),Jun Go(고준),Hong Joo Son(손홍주),Hee Seob Lee(이희섭),Young Jin Jung(정영진),Dae Youn Hwang(황대연) 한국생명과학회 2014 생명과학회지 Vol.24 No.6

미더덕껍질(Styela clava tunic, SCT)은 항염증 복합체, 창상필름, 골재생 유도 등을 포함한 다양한 의학적인 치료영역에 이용 되고 있다. 본 연구에서는 미더덕껍질 열수추출물(aqueous extract of Styela clava tunic, AE-SCT)의 H₂O₂에 의해 유발된 세포사멸의 보호 효과를 알아보기 위하여 세포 활성도의 변화에 관련된 요인을 측정하였다. 그 결과, AE-SCT는 3.3 mg/g의 플라보노이드와 32.3 mg/g의 페놀화합물을 포함하고 있었으며, HepG2 세포주에 독성을 유발하지 않았다. 또한, H₂O₂ 처리 후 AE-SCT를 처리하는 실험에서 AE-SCT는 H2O2에 의해 유발된 세포사멸을 개선하는 효과를 나타내지 않았다. 그러나, H₂O₂ 처리전에 AE-SCT를 사전 처리하는 예방효과 실험에서, 세포생존율은 H₂O₂만 처리한 그룹에 비하여 AE-SCT를 처리한 그룹에서 유의적으로 증가하였으며, 특히 AE-SCT를 50 μg/ml 처리한 농도에서 가장 높았다. 또한, FACS분석과 DAPI 염색에서도 사멸 세포의 수는 H₂O₂만 처리한 그룹에 비하여 AE-SCT를 처리한 그룹에서 유의적으로 감소하였다. 더불어, H₂O₂의 처리에 의해 유도된 Bax/Bcl-2 발현비율은 AE-SCT처리에 의해 농도의존적으로 감소되었다. 이러한 연구 결과는 AE-SCT가 H₂O₂에 의해 유발된 세포사멸을 예방하는 우수한 효과를 가지고 있음을 제시하고 있어 향후 다양한 항산화 제품 개발을 위한 기초자료로 사용될 수 있을 것으로 사료된다. Styela Clava tunic (SCT) has found some applications in many areas of medical treatment including as an anti-inflammatory compound, a wound healing film, in guided bone regeneration, and as a food additive. The protective effect of SCT aqueous extract (AE-SCT) on cell death induced by H₂O₂ treatment was investigated by measuring the changes in cell viability in HepG2 cells after AE-SCT treatment. High concentrations of antioxidant compounds including flavonoids (3.3 mg/g) and phenolics (32.3 mg/g) were detected in AE-SCT but no significant cytotoxicity was observed in HepG2 cells treated with AE-SCT. The viability of HepG2 cells was also not changed by treatment with different concentrations of AE-SCT after H₂O₂ treatment. However, cell viability was significantly increased in cells treated with three different concentrations of AE-SCT before H₂O₂ treatment. The greatest increase in cell viability was observed in the group treated with 50 μg/ml AE-SCT, when compared with vehicle-treated group. FACS and DAPI staining analysis indicated that the decrease in number of dead cells was dependent on the concentration of AE-SCT. Alterations in the Bax/Bcl-2 ratio after H₂O₂ treatment were significantly restored by treatment with different concentrations of AE-SCT. These results indicate that AE-SCT, which contains high levels of antioxidants, may protect cells against death induced by H₂O₂ treatment.

잠재적 생균제로서 식물 젖산균의 분리 및 특성

김정도 ( Jeong Do Kim ),박성보 ( Sung Bo Park ),이나리 ( Na Ri Lee ),정진하 ( Jin Ha Jeong ),이희섭 ( Hee Seob Lee ),황대연 ( Dae Youn Hwang ),이종섭 ( Jong Sup Lee ),정성윤 ( Seong Yun Jeong ),손홍주 ( Hong Joo Son ) 한국미생물생명공학회(구 한국산업미생물학회) 2011 한국미생물·생명공학회지 Vol.39 No.3

Plant lactic acid bacteria were isolated from plant-associated fermentative foods and crops, and their probiotic properties were investigated. Isolates K27 and O2 were isolated from Kimchi and onion, and identified as Lactobacillus plantarum on the basis of 16S rRNA gene analysis. The two strains were highly resistant to acid (an MRS broth at pH 2.5), where the survival rates of L. plantarum K27 and L. plantarum O2 were 90.2% and 97.3%, respectively. L. plantarum K27 and L. plantarum O2 also showed high bile resistance to 0.5% oxgall, with a more than 70% survival rate. They showed an inhibitory effect against pathogenic strains of Escherichia coli KCCM 40880 and Pseudomonas aeruginosa ATCC 10145. The antibacterial effect of the two strains was probably due to the presence of lactic acid. ACE inhibitory activities of the two strains ranged from 72.8% to 80.6% in MRS broth. Notably, the two strains showed high ACE inhibitory activity (89.2~98.2%) in MRS broth containing 10% skim milk. Antioxidant activity was tested by DPPH radical scavenging activity, with antioxidant activities of the strains being in the range of 56.8~61.5%. The results obtained in this study suggest that L. plantarum K27 and L. plantarum O2 may be potential probiotic starter cultures with applications with fermentative products.

흡수성이 우수한 창상치료용 미더덕껍질 셀룰로오스필름의 제조 및 특성 분석

성금용(Keum-Yong Seong),고은경(Eun-Kyoung Koh),이승현(Seunghyun Lee),곽문화(Moon Hwa Kwak),손홍주(Hong Joo Son),이희섭(Hee Seob Lee),황대연(Dae Youn Hwang),정영진(Young Jin Jung) 한국염색가공학회 2015 韓國染色加工學會誌 Vol.27 No.1

Isolation and Characterization of Lactic Acid Bacteria with Angiotensin-Converting Enzyme Inhibitory and Antioxidative Activities

박성보(Sung-Bo Park),김정도(Jeong-Do Kim),이나리(Na-Ri Lee),정진하(Jin-Ha Jeong),정성윤(Seong-Yun Jeong),이희섭(Hee-Seob Lee),황대연(Dae-Youn Hwang),이종섭(Jong-Sup Lee),손홍주(Hong-Joo Son) 한국생명과학회 2011 생명과학회지 Vol.21 No.10

본 연구에서는 ACE 저해능 및 항산화능이 있는 식물성 젖산균을 다양한 식물체로부터 분리한 후, 그 특성을 조사하였다. 김치, 부추, 포도 및 동동주에서 K-1, K-21, L-5, G-3 및 D-3 균주가 분리되었으며, 16S rRNA gene 염기서열 분석을 통하여 이들은 각각 Pediococcus pentosaceus, Lactobacillus plantarum, Weissella cibaria, L. plantarum 및 L. brevis로 동정되었다. 분리균주들은 MRS broth에서 44.3-71.9%의 ACE 저해능을 나타내었으며, 특히 G-3, L-5, K-1 균주는 skim milk가 함유된 MRS broth에서 59.0-8-98.6%의 높은 ACE 저해능을 나타내었다. 분리균주는 42.5-82.7%의 DPPH radical 소거능을 나타내었으며, G-3 및 K-1 균주는 pH 2.5의 인공위액에서 42.2-88.1%의 높은 생존율을 나타내었다. 분리균주는 0.3% oxgall에서 24시간 배양시 55.4-112.8%의 내성을 나타내었다. 또한 분리균주는 유기산 생성에 따른 pH 감소 효과로 인하여 Pseudomonas aeruginosa를 포함한 일부 병원성 세균의 생육을 억제할 수 있었다. In this study, we isolated and characterized plant-associated lactic acid bacteria which are able to produce angiotensin-converting enzyme (ACE) inhibitory and antioxidative activities. Five lactic acid bacteria were isolated from plants (grape and leek), a plant-associated fermentative product (Kimchi) and Korean traditional alcohol (Dongdongju). Strains K-1 and K-21 from Kimchi, strain L-5 from leek, strain G-3 from grape, and strain D-3 from Dongdongju were identified as Pediococcus pentosaceus, Lactobacillus plantarum, Weissella cibaria, L. plantarum, and L. brevis, respectively, by 16S rRNA gene analysis. ACE inhibitory activities of isolated strains ranged from 44.3 to 71.9% in the MRS broth. G-3, L-5 and K-1 strains especially showed high ACE inhibitory activities (59.8-98.69%) in the MRS broth containing skim milk. DPPH radical scavenging activities of the strains were in the range of 42.5-82.7%. All strains showed varying levels of resistance in artificial gastric fluid (pH 2.5), retaining viability ranging from 42.2 to 88.1% after 3 hr of incubation. All strains showed high resistance to 0.3% oxgall after 24 hr of incubation; survival rates were in the range of 55.4-112.8%. Isolated strains were found to be antagonistic to some pathogens including Pseudomonas aeruginosa.

나노세라마이드의 캡슐화와 아토피 피부의 치료

조춘구 ( Choon-koo Zhoh ),김인영 ( In-young Kim ),이희섭 ( Hee-seob Lee ) 대한화장품학회 2004 대한화장품학회지 Vol.30 No.3

나노 세라마이드의 캡슐화(Nano-ceramide capsulation)는 세라마이드 III와 토코페릴리놀레이트를 나노 크기의 모노 베지클에 캡슐화시켜 피부각질층에 작용하도록 만든 기술이다. 그 제조에는 고압 마이크로플루다이저를 사용하였으며 조성은 다음과 같다. 모노 베지클의 막 강화제로서 0.5~5.0 wt%의 수소첨가레시친과 0.1~2.0 wt%의 리소레시친을 사용하였으며, 용제로서 5.0~10.0 wt%의 propylene glycol과 5.0~10.0 wt%의 ethanol을 사용하였다. 피부 보습기능과 아토피 치료를 위하여 활성성분인 세라마이드 III와 토코페릴리놀레이트를 사용하였으며 유화제는 함유하지 않았다. 나노 세라마이드 캡슐기술의 최적조건은 다음과 같다. 마이크로플루다이저의 통과압력은 1,000 bar, 통과횟수는 3회, 통과 온도는 60~70℃가 적당하였다. 또한, nano capsule의 pH는 5.8±0.5이었다. 평균입자크기는 63.1±7.34 nm로 물과 같은 투명한 성상을 보였으며, 제타포텐셜값은 -55.1±0.84 mV이었다. 임상실험 결과로서, 피부보습효과(in-vivo, n=8, p-value<0.05)는 비교시료보다 21.15% 개선되었으며, 치료 전보다는 36.31% 개선되었다. 더구나, 아토피 피부 효과는 아토피 피부 환자 10명에게서 양성반응을 보였다. The nano-ceramide capsulation is a technique that capsulates ceramide III and tocopheryl linoleate at the mono-vesicle to act on the horny layer in skin. In this technique, 0.5~5.0 wt% of hydrogenated lecithin and 0.01~2.00 wt% of lysolecithin are used as the membrane-strengthen agents of the mono-vesicle and 5.0~10.0 wt% of propylene glycol and 5.0~10.0 wt% of ethyl alcohol are used as solvents. Active ingredients such ceramide III and tocopheryl linoleate are utilized to enhance the moisturizing efficacy and treat atopy skin. These materials do not contain synthetic emulsifiers. The optimal conditions or nano-ceramide capsulation are such that particles pass Microfludizdizer 3 times at 1,000 bar and 60~70℃ and pH of nano capsules is 5.8±0.5. The average size of particles is 63.1±7.34 nm showing lucid state like water by the laser light scattering. A zeta potential value is -55.1±0.84 mV. Through clinical tests, the moisturizing effect (in-vivo, n=8, p-value<0.05) showed 21.15% of improvement comparison to comparison-samples and 36.31% of improvement compared to the state before treatment. Moreover, the effectiveness of atopy skin showed positive reaction from 10 volunteers.

삼백초 건근을 첨가한 국수의 품질 특성

박정은 ( Jung Eun Park ),김민정 ( Min Jeong Kim ),박소해 ( So Hae Park ),이희섭 ( Hee Seob Lee ) 부산대학교 김치연구소 2011 김치의 과학과 기술 Vol.14 No.-

본 연구에서는 삼백초 건근 분말을 새로운 식품소재로 활용하고자 하는 연구의 일환으로 밀가루에 삼백초 건근 분말 첨가량을 달리하여 국수를 제조한 후 품질특성을 분석하였다. 삼백초 첨가량이 증가할수록 색도의 L값과 b값은 감소하였으며, a값은 증가하였다. 조리특성에 있어서 무게와 부피가 증가하였으며, 또한 국물의 탁도도 증가하여 조리중 고형분의 손실량이 증가함을 알 수 있었다. 조직감은 생면의 경우 전반적으로 증가하는 경향을 보이는 반면, 조리면에 있어서는 경도를 제외하고는 큰 차이가 나지 않았다. 관능검사 결과 외관 및 향은 유의적인 차이가 없었으나, 맛, 조직감은 삼백초 건근 분말의 첨가량이 증가함에 따라 감소하는 경향을 나타내었고 종합적 기호도에서 삼백초 건근 분말을 3%까지 첨가할 경우 대조군과 유의적인 차이가 나지 않았다. 따라서 삼백초 건근 분말을 첨가하여 국수를 제조할 경우 3% 첨가가 적절한 함량으로 판단된다. The objectives of this study were to evaluate the quality characteristics of noodles added with dried Saururus chinensis Baill. root powder (SC powder), thereby determining which noodle recipe is the most preferable. When the amount of SC powder was increased, both L and b color values decreased, whereas a value was increased. The weight, and volume of cooked noodles increased, as did the turbidity of the soup. In the case of textural properties, addition of SC powder in noodle increased hardness, cohesiveness, springiness, chewiness, brittleness, and gumminess. Overall, according to the results of the sensory evaluation, the noodle added with SC powder up to 3% was the most preferable.