http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
유체전단응력에 의하여 3T3-L1 지방세포가 받는 영향
이정근,이영훈,진희원,이서현,김지현,Lee, Jeongkun,Lee, Yeong Hun,Jin, Heewon,Lee, Seohyun,Kim, Chi Hyun 대한의용생체공학회 2018 의공학회지 Vol.39 No.4
Adipocytes affect obesity through the regulation of lipid metabolism. Physical loading is an important regulator of fat tissue. There are ongoing in vitro studies inducing mechanotransduction on 3T3-L1 preadipocytes with mechanical stimulus in order to treat obesity by inhibiting adipogenesis and provoking cell death. In this study, our goal was to suggest a new therapy for obesity by investigating whether fluid shear stress (FSS) changes transcription factors on 3T3-L1 related with adipogenesis and cell death. FSS loading was applied to 3T3-L1 preadipocytes at 1Pa and 1Hz. After loading, bright field images were taken and an immunofluorescence assay was conducted to observe actin stress fiber formation. Western blot analysis was conducted to identify the activation of the ERK pathway as well as the adipogenic factors, which including C/EBPs and $PPAR{\gamma}$. The expression of osteopontin, a protein related to inflammation in adipose tissue, and cell death related factors, Bax, Bcl-2, and Beclin, were also measured. Results showed that FSS stimulated the formation of actin stress fibers in 3T3-L1 and also that the activation of C/EBPs decreased significantly when compared with the control group. $PPAR{\gamma}$ activation in the 2 hour FSS group was lower than the 1 hour FSS group, which implied that the results were time dependent. Additionally, there were no differences in the expression of cell death factors after FSS loading. In summary, similar to other fibroblasts, the formation of actin stress fibers induced by mechanotransduction may affect the differentiation of 3T3-L1, leading to inhibition of adipogenesis and inflammation.
수계 바인더를 이용한 W-Cu 합금의 새로운 사출성형법 연구
이정근,Lee Jeong-Keun 한국분말야금학회 2006 한국분말재료학회지 (KPMI) Vol.13 No.1
The present work illustrates the use of water-soluble cupric salts as ingredients of binder for injection molding of $W-10 wt\%$ Cu. Parts produced are dense, homogeneous and have good surface finish, compared to those produced using conventional binder system. This new binder system provides also process-simplification benefit. $CuCl_2\;and\;Cu(NO_3)_2$ with the purity of $98\%$ was selected for this study. Rapid sintering process involving thermal decomposing was successful in densification for 1h. Final density that is about $93\%$ of theoretical value could be obtained, and are distinguishable from conventionally processed W-Cu composites.
3T3-L1 지방전구세포에서 멜라토닌과 유체전단응력의 영향
이정근,이영훈,박채림,김지현,Lee, Jeongkun,Lee, Yeong Hun,Park, Chae Lim,Kim, Chi Hyun 대한의용생체공학회 2018 의공학회지 Vol.39 No.3
Obesity is a worldwide disease caused by the excessive proliferation of adipocytes. Multiple factors, including melatonin and physical loading, are involved in the control of obesity. Melatonin has been shown to induce apoptosis on preadipocytes while physical loading such as fluid shear stress (FSS) affects the proliferation and differentiation of adipocytes. Here, we studied the combined effects of melatonin and FSS on 3T3-L1 preadipocytes. For physical loading, preadipocytes were stimulated with a maximum dynamic fluid shear stress of 1 Pa at 1 Hz for 2 hours with/without melatonin. The experiment conditions were divided into four groups: (1) control, (2) 1 mM melatonin treatment, (3) FSS, and (4) combined 1 mM melatonin and FSS. All groups had a fixed duration time of 2 hours. ERK, p-ERK, COX-2, $C/EBP{\beta}$, $PPAR{\gamma}$, osteopontin, Bax, caspase-3 and caspase-8 proteins were assessed by Western blot analysis. GAPDH was used as a control. Results showed that combined melatonin and FSS treatment activated the ERK/MAPK pathway but not COX-2. Furthermore, combined melatonin and FSS treatment significantly decreased $C/EBP{\beta}$ and $PPAR{\gamma}$ compared to other groups. However, caspase-3 and caspase-8 did not result in significant changes. In summary, combined melatonin and FSS appears to have the potential to inhibit adipogenesis and treat obesity.
온간성형용 분말의 고온 유동도와 겉보기 밀도 측정에 관하여
이정근,김순욱,Lee Jeong-Keun,Kim Soon-Wook 한국분말야금학회 2006 한국분말재료학회지 (KPMI) Vol.13 No.1
The aim of this work was to establish an optimal condition for determination of apparent density and flow rate for warm compacting powder. For this purpose it was evaluated uncertainty on them according to ISO Guide to the Expression of Uncertainty in Measurement. This evaluation example would be useful even in powder fluidity measurement at room temperature.
저압주조품의 미세조직과 기계적 성질에 미치는 금형온도의 영향
이정근,박종성,김명호 ( Jeong Keun Lee,Chong Sung Park,Myung Ho Kim ) 한국주조공학회 1998 한국주조공학회지 Vol.18 No.3
N/A Microstructure and mechanical properties of the low pressure die-cast Al wheels were investigated by microscope, image analyzer, NDT (non-destructive test), and tensile test. The variation of SDAS (secondary dendrite arm spacing), porosity per unit area, quality grade, and tensile properties with the mold temperatures were examined. SDAS was gradually decreased with a decrease in temperature. However, the lowest value of porosity per unit area was observed at the mold temperature of 405˚C and the optimum mold temperature was found to be 405˚C. Besides, from the observation of pore morphology, it was also found that the pore formation was mainly caused by shrinkage during solidification. The tensile strength, elongation, and impact toughness were markedly decreased, however the yield strength was nearly constant. The decrease of mechanical properties is attributed to the increase of porosity. (Received January 23, 1998)