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오염수 내의 유기인 화합물의 측정을 위한 광섬유 바이오센서 ( 제 1부 : 장치 설계 및 개발 )
최정우,민준홍,이원홍 ( Jeong Woo Choi,Jun Hong Min,Won Hong Lee ) 한국센서학회 1994 센서학회지 Vol.3 No.2
Fiber-optic biosensor for the detection of organophosphorus compounds in a contaminated water was developed, which was the component of pesticides and agricultural agent. The detection principle of designed sensor was the pH variance induced by a reaction of acetylcholinesterase enzyme inhibited by organophosphorus compounds. The pH variance was detected by the optical system to measure the organophosphorus compounds. Litmus was selected as the pH-sensitive dye suitable to the enzyme reaction and a light source to be detected by the optical system. The enzyme entrapped in Ca-alginate gel was immobilized at the inner wall to maintain the high activity of enzyme and to be reused for a long period. The optical fiber was used to miniaturize and control remotely the sensor system. The He-Ne laser with 632 nm was selected as the light source to prevent light intensity fluctuation by the product. Cheap plastic optical fibers were used as the transmission part of the light and the phototransistor was used as the reception part of light based on the wavelength of He-Ne laser. The proposed fiber-optic biosensor has the linear analytical range of 0 ppm-1.5 ppm with response time of 5 minutes.