Aspergillus ochraseus의 미세구조(微細構造) 및 바이러스 감염(感染)에 관(關)한 연구(硏究)

등영건,유영선,이배함,Deung, Young-Kun,Lew, Young-Sern,Lee, Bae-Ham 한국현미경학회 1975 Applied microscopy Vol.5 No.1

These studies were carried out to detect the presence of infected virus- like particles and also were observed the ultrastructures of Aspergillus ochraseus isolated from kokja and Korean ginseng. The results of ultrastructures of Aspergillus ochraseus are summarized as follows: 1. In fungal cells, nuclei were enclosed by a irregular double membrane and nucleoli in the nucleus. 2. In cytoplasm, mitochondria, rough endoplasmic reticulum with ribosomes and glycogen were scattering distributed and many lomasomes also observed. 3. The osmiophilic bodies of fungal cells existed in the vesicles. 4. The cell walls were composed of a low electron dense materials. 5, Conidia cell walls were extremely thick and possessed the high electron density of outer coat. The virus-like particles were observed in the hyphae of Penicillium chrysogenum Q-176. These virus-like particles measured $350{\AA}$ in diameter. But strains of Aspergillus ochraseus, showing some vesicle particles were also observed about $800{\AA}$ in diameter in the central region of young fungal hyphae. Based on the results of these experiments, it can not be determined virus particles or not. The further studies to determination of virus particles will be proceeded by the chemical, physical and biological assay methods.

가스크로마토그래피에 의한 엽연초 중의 니코틴 정량 연구

원충택 ( Choong Taik Won ),이배함 ( Bae Ham Lee ),박면용 ( Myon Yong Park ) 건국대학교 기초과학연구소 1976 理學論集 Vol.2 No.-

가스크로마토그래프에 열분해 연소로를 부착시켜 엽연초를 직접 열분해시킨 다음 가스크로마토그래프의 충진관에 분해가스를 도입하여 분석하는 방법을 사용하여 니코틴을 정량하였다. 이 방법을 사용하므로서 추출하여 정량하는 방법보다 신속 정확하게 정량할 수 있었다. 열분해법에 의하여 정량할 때 시료량, 열분해온도의 범위, 열분해시킬 때 사용하는 타르흡수, 장치 등에 대하여 검토하였다. 그리고 이 방법은 자연물질 중의 휘발성분 정량에도 이용할 수 있음을 알았다. The pyrolyzing technique of the samples was applied to a gas chromatographic analysis of nicotine component in tobacco leaves. A pyrolyzing temperature of natural product components, which is the plateau region of the curve obtained by plotting pyrolysis temperature versus gas chromatogram peak area, was found to be the most suitable temperature. This pyrolyzing temperature range coinsides with the pyrolyzing temperature determined by a thermogravimetric balance method. The optimum conditions for the determination of nicotine in tobacco leaves are as the following: pyrolyzing temperature; 350-550°C, carrier gas (nitrogen) flow rate; 75 ml/min., column (182×0.3 cm); packed with packing material of liquid phase (SE-30) 10% on Chromosorb (80-100mesh), oven temperature; 1500˚C.

시판생약의 진균분포에 관한 연구

용만중(Mahn Joong Yong),최병현(Byung Hyun Choi),박재주(Jae Joo Park),이배함(Bae Ham Lee) 한국생약학회 1979 생약학회지 Vol.10 No.3

21 herbal drugs registered in K.P. III were tested for contamination of fungi and isolation of aflatoxin producible strains. Initially contaminated fungi were Aspergillus group (41.28%) and Penicillium (47.26%) and the other fungi were contaminated somewhat. The most frequent isolation of Aspergillus group was Cnidii Rhizoma and that of Penicillium was Piperis Fructus nigri. Cnidii Rhizoma was the most contaminated drug and Cassiae Cortex was the least among them. Aspergillus flavus was isolated from 10 samples and Aspergillus parasiticus was detected in Glycyrrhizae Radix, Phellodendri Cortex. Aspergillus ochraceus was isolated from only Scutelariae Radix, and Fusarium nivale was isolated from Cnidii Rhizoma and Torreya Semen. None of Aspergillus and Penicillium was detected in only Coptidis Rhizoma. No strains of Aspergillus flavus and Aspergillus parasiticus isolated from were produced aflatoxin.

시판 생약의 진균 분포에 관한 연구 (1)

용만중(Mahn-Joong Yong),최병현(Byung-Hyun Choi),박재주(Chae-Joo Park),이배함(Bae-Ham Lee) 한국생약학회 1980 생약학회지 Vol.11 No.1

생약제는 천연물로서 생산과정, 저장 조건 등에 의하여 진균의 오염이 가능하며 품질관리 및 국민보건상 문제가 될 수 있다. 이에 따라 본연자들은 대한 약전수재 생약 21종을 취하여 진균의 분류 및 aflatoxin 생성유무를 검색할 목적으로 연구하였으며 현미경상에 목측하여 Raper and Fennell, 小田, 坂口의 방법에 의해 동정하였으며 Showell 방법에 준하여 aflatoxin analysis를 한 바 다음과 같은 결론을 얻었다. 1) 본 실험에서 광범위하게 분리된 진균은 Aspergillus group과 Penicillium이었으며 전체의 88.5%를 차지하였다. 또한 Mucor, Rhizopus, Alternaria sp., Fusalium sp. 등이 약간 분리되었다. 2) Aspergillus group이 가장 많이 분리된 생약은 후수였으며 Penicillium이 가장 많이 분리된 생약은 천궁이었다. 3) Aspergillus group과 Penicillium이 전혀 분리되지 않은 생약은 황련이었으며 겨자에서는 Aspergillus group이 계피, 길경, 건강에서는 Penicillium이 분리되지 않았다. 4) Aflatoxin 생성가능균주인 Aspergillus flavus는 10종의 생약에서 분리되었으며 Aspergillus parasiticus는 감초, 황백에서만 분리되었다. 5) Ochratoxin 생성가능균주인 Aspergillusochraceus는 황금에서만 분리되었으며 fusariogenin 생성가능균주인 fusarium nivale은 천궁과 비자에서만 분리되었다. 6) 공시 생약에서 분리한 Aspergillus flavus 22주, Aspergillus parasiticus 2주를 random sampling하여 aflatoxin분리 실험하였던 바 aflatoxin을 분리할 수가 없었다.

韓國의 Mycoparasitic Fungi에 관하여 : Part I. Mycoparasitic Fungi on the Pathogen of Powdery Mildow 第一報 흰가루 病菌에 寄生하고 있는 眞菌

李培咸 建國大學校附設 應用科學硏究所 1976 理學論集 Vol.2 No.-

Two hyperparasitic fungi, previously not recorded in Korea, were found from powdery mildew on Cucurbita moschata Duch. and on Cucumis melo L. var. makuwa Makino, and these two fungi were identified as Ampelomyces quisqualis Ces. through the study of morphological characters. The pathogen of powdery mildew on Cucurbita moschata Duch. in Korea was reported by Lee(5) to be Sphaerotheca fuliginea(Sch1.) Salm. The other pathogen of powdery mildew on Cucumis melo L. var. makuwa Makino was also identified as Sphaerotheca fuliginea(sch1.) Salm. and it was not reported in Korea up-to-date.

健全葉과 稻熱病 罹病葉에 있어서의 Free Amino Acid의 定量的 比較

李培咸,白壽鳳 건국대학교 1968 學術誌 Vol.9 No.1

This experiment was undertaken the purpose of investigating the difference of the content of Free Amino Acids in the diseased and healthy leaves of the resistance and susceptible rice varieties to Blast by means of Paper Chromatography. The results obtained are summarized as follows. (1) In the healthy and diseased leaves, the former was founded eight to ten sorts of Free Amino Acids and later was observed eleven. In the both of leaves, Alanine and Valine were contained in larger amount, and Aspartic Acid, Glycine and Arginine were founded relatively less in general. (2) In the diseased leaves, the Amino Acids were increased in quantity, especially Glutamine and Aspartic Aacid were remarkable, and Cystine were founded only in the diseased ones. (3) It seems to that the healthy leaves in all varieties were observed the Amino Acid contents in notably increase and decrease, but in the diseased ones were not changed in amount. Tatacho, susceptible variety, was more contained the amount of Glutamine and Aspartic Acid than Zenith, resistance one.

微生物의 利用과 人間生活

李培咸 건국대학교 1973 文理論叢 Vol.2 No.1

Productivity of Aflatoxin by Korean Aspergilli

Lee, Bae-Ham 건국대학교 1974 學術誌 Vol.18 No.1

A total of 86 strains of Aspergillus flavus isolated from home·made fermented foodstuffs, were cultured on steamed rice and the productivity of aflatoxin was studied by flurorometry and thin layer chromotography, using an aflatoxin producing strain ATCC 17715 as the control. Seven strains of the tested 86 strains of Asp.flavus produced aflatoxins to some degree on the medium. Two strains produced 4 kinds aflatoxins, B1, B2, G1 and G2, as the control strain did, while the other 5 strains produced the B group of aflatoxins only.

硫黃細菌에 의한 金屬 製鐵 및 加黃고무의 脫黃 再生에 관한 硏究

李培咸,全英淵,丁聖九 건국대학교 1970 學術誌 Vol.11 No.1

In this paper the important results obtained on properties of culture condition for iron-oxidizing to sulfide ores and discharge sulfur in Rubber treated by Ferrobacillus ferrooxi-dans-28 and Thiobacillus thiooxidans-4 which isolated soil are as follows : ① F. ferrooxidans-28 is completly oxidized to Fe2+ for incubates at 25℃, three days in K-medium ② The optimum temperature for iron-oxidizing and growth rate of F. ferrooxidans -28 is range 20-30℃ and optimal pH, the range 1.5-3.0. ③ Bacterial action are most effected in K-medium contaning 400-600ppm Nitrogen. ④ T. thiooxidans-4 is prominently discharged sulfur in Rubber. ⑤ Bacterial sulfur dicharge rate attained 24% for 4-days, 30℃ incubation. ⑥ And it seems to belike that the smaller rubber particle size, the easier to dicharged sulfur become.

植物病院微生物의 新變異型 出現의 機作에 對한 硏究

李培咸,白壽鳳,羅民根,李喜茂 건국대학교 1966 學術誌 Vol.7 No.1

The experiment was undertaken with purpose of investigating some mechanisms in occuring of the variation of several pathogenic microorganisms on their host plant. The strains of Helminthosporium oryzae, Fusarium solani and Alternaria porri were isolated by single spore method, grown and observed the state and movement of nuclei in their various parts, at the Laboratory microbiology, the College of Literature and Science, Kon Kuk University, Seoul. The results obtained were summarized as follows. It was observed that the more than two nuclei and they were gathered with moving in a cell and appeared Anastomosis in the asexual stage such as the germination of conidium, growing hyphae and in conidial formation, and was thought that these phenomena will be effected as a cause of variation in these microorganisms.