황연(黃連)이 Lipopolysaccharide 뇌실 주입으로 유발된 생쥐의 IL-6와 $TNF-{\alpha}$ 변화에 미치는 영향

심은영,윤정문,이태희,Sim Eun-Yeong,Yun Jeong-Moon,Lee Tae-Hee 대한한의학방제학회 2004 大韓韓醫學方劑學會誌 Vol.12 No.1

Objective: This study was conducted to investigate the effects of Coptidis Rhizoma on the plasma IL-6 and $TNF-{\alpha}$ level in mice by intracerebroventricular(I.C.V.) injection of Lipopolysaccharide (LPS). Method: 6 mice were assigned to each of the Normal group, the Control group, and the individual Experimental groups. In the Normal group only saline was administered intragastrically, and in the Control group LPS was injected intracerebroventricularly 1 hr after intragastric administration of saline. In the Experimental groups Coptidis Rhizoma(0.5g/kg, 1.0g/kg, 3.0g/kg) was administered intragastrically to mice 1 hr prior to LPS (100ng/mouse) I.C.V. Injection. To measure the plasma IL-6 and $TNF-{\alpha}$ level of mice, their blood samples were collected from retro-orbital venous plexus, immediately centrifuged at $4^{\circ}C$, and plasma was removed and stored frozen at $-83^{\circ}C$ for later determination of plasma IL-6 and $TNF-{\alpha}$. Result: 1. LPS I.C.V. Injection increased plasma IL-6 level significantly in a dose-dependent manner compared with Normal group. (P<0.01) The plasma IL-6 concentration reached a significant maximal level about 1 hr after LPS(100ng/mouse) I.C.V. Injection.(P<0.001) 2. Both the 0.5g/kg(Sample A) and 1.0g/kg(Sample B) groups to which Coptidis Rhizoma was administered intragastrically 1 hr prior to LPS(100ng/mouse) I.C.V. Injection showed insignificant lower plama IL-6 level in 1 hr than Control group(P>0.05), and 3.0g/kg group(Sample C) conversely showed higher plama IL-6 level than Control group. 3. LPS I.C.V. Injection increased plasma $TNF-{\alpha}$ level significantly in a dose-dependent manner compared with Normal group.(P<0.05) The plasma $TNF-{\alpha}$ concentration reached a significant maximal level about 1 hr after LPS(100ng/mouse) I.C.V. Injection.(P<0.001) 4. All Sample groups(0.5g/kg, 1.0g/kg, and 3.0g/kg) to which Coptidis Rhizoma was administered intragastrically with each constituent-dose 1 hr prior to LPS(100ng/mouse) I.C.V. Injection showed significant lower $TNF-{\alpha}$ plama level in 1 hr than Control group.(P<0.001) These data revealed that Coptidis Rhizoma might have anti inflammatory effect by reducing the plasma $TNF-{\alpha}$ level in a dose dependent manner in mice LPS I.C.V. Injection.

부자(附子)가 Lipopolysaccharide의 뇌실 주입으로 유발된 생쥐의 혈중 IL-6와 $TNF-{\alpha}$ 변화에 미치는 영향

고동균,윤정문,이태희,Koh Dong-Kyun,Yun Jeong-Moon,Lee Tae-Hee 대한한의학방제학회 2004 大韓韓醫學方劑學會誌 Vol.12 No.1

Objective: This study was conducted to investigate the effects of Aconiti Tuber on the plasma IL-6 and $TNF-{\alpha}$ level in mice stimulated by intracerebroventricular(I.C.V.) Injection of Lipopolysaccharide(LPS). Method: 6 mice were assigned to each of the normal group, the control group, and the experimental group. In the normal group only saline was administered intragastrically, and in the control group LPS was injected intracerebro-ventricularly 1 hr after intragastric administration of saline. In the experimental groups (Aconiti Tuber 0.5g/kg, Aconiti Tuber 1.0g/kg, Aconiti Tuber 3.0g/kg) each sample was administered intragastrically to mice 1 hr prior to the stimulation by LPS I.C.V. Injection. To measure the plasma IL-6 and $TNF-{\alpha}$ level of mice, their blood samples were collected from retro-orbital plexus, immediately centrifuged at $4^{\circ}C$, and plasma was removed and stored frozen at $-83^{\circ}C$ for later determination of plasma IL-6 and $TNF-{\alpha}$. Result : 1. LPS I.C.V. Injection increased plasma IL-6 level significantly in a dose-dependent manner compared with normal group(P<0.01). The plasma IL-6 concentration reached a significant maximal level about 1 hr after LPS I.C.V. Injection(P<0.001). LPS I.C.V. Injection increased plasma $TNF-{\alpha}$ level significantly in a dose-dependent manner(P<0.05). The plasma $TNF-{\alpha}$ concentration reached a significant maximal level about 1 hr after LPS I.C.V. Injection(P<0.001). 2. Sample A group to which Aconiti Tuber(0.5g/kg) was administered intragastrically 1 hr prior to the stimulation by LPS I.C.V. Injection showed insignificant lower plasma IL-6 level in 1 hr than control group(P<0.0635), and sample B group (Aconiti Tuber 1.0g/kg) showed significant lower plasma IL-6 level in 1 hr than control group(P〈0.05), and sample C group (Aconiti Tuber 3.0g/kg) showed significant lower IL-6 plasma level in 1 hr than control group(P<0.01). 3. sample A group to which Aconiti Tuber(0.5g/kg) was administered intragastrically 1 hr prior to the stimulation by LPS I.C.V. Injection showed insignificant lower plasma $TNF-{\alpha}$ level in 1 hr than control group(P>0.05), and Both sample B(1.0g/kg) and sample C(3.0g/kg) groups showed significant lower $TNF-{\alpha}$ plasma level in 1 hr than control group(P<0.01). These data revealed that Aconiti Tuber might have the anti inflammatory effect by reducing the plasma IL-6 and $TNF-{\alpha}$ level in a dose dependent manner in mice LPS I.C.V. Injection.

발효우슬, 두충, 당귀 복합물의 Primary Chondrocyte 항염 효과 연구

김다경(Dakyung Kim),이다솜(Dasom Lee),윤정문(Jeong Moon Yun),정현철(Hyun Cheol Jeong),이성진(Sung-Jin Lee),손요한(Johann Sohn),이정민(Jeongmin Lee) 한국식품영양과학회 2020 한국식품영양과학회지 Vol.49 No.6

골관절염은 통증을 동반한 만성질환으로 염증성 cytokines, 금속단백분해효소, 단백질가수분해효소 등이 복합적으로 관여한다. 골관절염의 주요 치료 목표는 관절 운동유지 및 통증감소이며 치료 약물로는 주로 비스테로이드성 항염제를 처방하고 있으나, 이러한 약물은 장기 복용 시 부작용이 따른다는 보고가 있어 장기간 복용이 가능한 건강기능식품 개발이 활발히 일어나고 있다. 본 연구에서는 관절염에 효능이 알려진 우슬, 두충, 당귀를 소재로 선정하였고 그중 우슬을 Lactobacillus plantarum으로 발효시켰으며, 이를 복합물로 사용하여 연골세포의 항염증 효능을 확인하고자 연구를 진행하였다. Primary 한 연골 세포에 발효우슬, 두충, 당귀 복합물을 처리한 후 NF-κB와 COX-2 유전자 발현, 염증성 cytokine의 활성 및 유전자 발현, MMPs 유전자 발현, 연골 형성 인자의 유전자 발현 등을 측정하였다. 그 결과 발효우슬, 두충, 당귀 복합물 처리 시 MMP 발현(MMP-3, MMP-13)을 감소시키고 aggrecan 및 collagen type Ⅱ의 발현은 증가시켜 연골 보호 효과에 대한 활성을 확인하였으며, 염증 반응에 관하여 NF-κB 활성 및 염증성 cytokines 의 발현을 억제시켜 항염증 효과에도 영향을 미친 것을 확인하였다. 따라서 발효우슬, 두충, 당귀 추출 복합물이 연골세포에서 항염증 효과를 가진 천연 기능성 식품의 개발에 있어 기초자료로 활용될 수 있을 것으로 기대할 수 있다. Osteoarthritis (OA) is a chronic disease characterized by degeneration and cartilage pain. Improvement of joint health using functional materials has become an area of active research due to the side effects of drugs used in treatments. Fermented Achyranthes japonica Nakai, Eucommia ulmoides Oliver, and Angelica gigas Nakai compounds (FAEAC) are known to have anti-inflammatory and cartilage protection effects, and fermentation is known to potentiate active ingredients. The purpose of this study was to investigate the inhibitory effects of FAEAC on arthritis using chondrocytes. An animal model of arthritis H₂O₂ and LPS were used to cause apoptosis and inflammatory reactions in chondrocytes. Pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), catabolic (PGE2, NF-κB, COX-2, MMPs), and anabolic (aggrecan, collagen type Ⅱ) factors were measured by PCR, ELISA, and morphological analyses. FAEAC in chondrocytes significantly increased expression of aggrecan and collagen type Ⅱ, and activities of NF-κB, COX-2, and MMPs as well as secretion of cytokines (TNF-α, IL-1β) significantly decreased. In conclusion, FAEAC seemed to be active in cartilage protection by reducing factors associa.

자외선에 의한 피부 보습 감소에 양태반이 미치는 영향

박수정(Soo-Jeung Park),이민희(Minhee Lee),윤정문(Jeong-Moon Yun),김다경(Dakyung Kim),김옥경(Ok-Kyung Kim) 한국식품영양과학회 2019 한국식품영양과학회지 Vol.48 No.1

본 연구에서는 양태반이 자외선에 의해 감소한 피부보습에 미치는 영향을 살펴보고자 양태반 분말 식이를 투여한 UVB 조사 동물의 피부조직을 관찰하였다. 그 결과 양태반 분말식이를 투여한 동물에서는 UVB에 의한 procollagen type Ⅰ, collagen type Ⅰ, elastin, fibrillin-1의 mRNAs 발현 감소를 유의적으로 억제시켰으며 HAS2 mRNAs 발현 감소 또한 억제되었다. 동물의 등 피부에서 경피 수분 함량을 측정하여 양태반 분말의 섭취가 피부 수분 함량을 증가시켰음을 확인하였다. 따라서 이러한 결과를 통하여 양태반의 피부보습 작용을 확인함으로써 기능성 식품 소재로서의 가능성을 확인할 수 있었다. We investigated the potential effects of lamb placenta on UVB irradiation-induced decreases in skin hydration in vitro and in vivo. We found that UVB irradiation suppressed the mRNAs expression of procollagen typeⅠ, collagen type Ⅰ, elastin, fibrillin-1, and hyaluronan synthase 2 in back skin from UVB-irradiated hairless mice. In addition, UVB irradiation decreased the skin hydration in hairless mice. However, dietary supplement of lamb placenta inhibited the decreases in the mRNAs expression of procollagen type Ⅰ, collagen type Ⅰ, elastin, fibrillin-1, and hyaluronan synthase 2 and the skin hydration in UVB-irradiated hairless mice. Moreover, we found that lamb placenta treatment increased hyaluronan production while UVB irradiation decreased hyaluronan production in UVB-irradiated HaCaT cells. Therefore, these results confirmed the possibility that lamb placenta could be developed as a functional food material to increase skin moisturizing.

가자, 암라 추출복합물(FBF-TC)의 분화된 3T3-L1 세포와 고지방식이로 유도된 비만동물모델에 대한 항비만 효과

박수정(Soo-Jeung Park),이민희(Minhee Lee),윤정문(Jeong-Moon Yun),김다경(Dakyung Kim),임현정(Hyunjung Lim) 한국식품영양과학회 2019 한국식품영양과학회지 Vol.48 No.1

본 연구에서는 가자와 암라 복합추출물(TP)이 분화된 3T3-L1 세포와 고지방식이로 유도된 비만동물모델에 미치는 항비만 효과를 관찰하였다. 분화된 3T3-L1 세포에 TP 0~80 μg/mL 농도를 처리하여 lipid accumulation, TG, glycerol, cAMP, 그리고 지방분해 관련 유전자들을 측정한 결과, 40 μg/mL 이상의 농도에서 세포 내 축적된 지방구들이 분해되어 glycerol 방출량을 증가시켰고, PDE3B의 발현을 감소시켜 cAMP가 가수분해 되지 않게 조절하여 PKA, HSL, 그리고 ATGL의 발현을 증가시켰으며, 지방구를 둘러싸고 있는 Perilipin의 발현을 감소시킨 것으로 보아 PKA를 촉진하는 cAMP 경로의 지방분해 기전이 작용하였음을 확인하였다. 고지방식이로 비만을 유도한 C57BL/6J 마우스를 이용하여 항비만 활성을 관찰한 결과, TP의 섭취가 체중증가량, 장간막 지방, 피하지방, 그리고 혈액 내 TG, TC, LDL-cholesterol, leptin의 함량을 대조군에 비해 농도 의존적으로 감소시켰고, HDL-cholesterol 및 adiponectin의 함량을 유의적으로 증가시킨 것으로 보아 TP가 비만 관련 호르몬 및 지방분해 효소들을 조절한 것으로 여겨진다. 특히 동물모델에서 단독추출물보다 복합추출물일 때 지방분해에 있어 시너지효과가 나타난 것을 확인하였다. 따라서 TP가 지방분해 기전인 cAMP 경로를 활성화하고 관련 유전자와 효소들을 조절하여 최종적으로 체중감소에 도움이 되는 원료로 기능성이 입증되어 항비만 활성에 대한 기초자료로의 활용을 기대할 수 있다. Abnormal fat accumulation on the body causes chronic disease known as obesity and is a serious problem in today’s society. We investigated the anti-obesity effects of combined extracts of Terminalia chebula (T) and Phylanthus emblica (P) (TP; FBF-TC) in vitro and in vivo. 3T3-L1 preadipocytes were differentiated for 9 days. On the last day of differentiation, Oil red O staining, glycerol release, intracellular cAMP levels, and lipolytic enzymes were measured. Compared to the control, lipid accumulation, and mRNA expressions of PDE3B and Perilipin were significantly decreased in the presence of TP 80 μg/mL. The levels of free glycerol, intracellular cAMP, and mRNA expressions of HSL and ATGL were significantly increased by 139%, 99.5%, 65.6%, and 100.8%, respectively, in the presence of TP 80 μg/mL. In the animal study, C57BL/6J mice were tested for 12 weeks after being separated into eight groups: Normal diet group (ND), high fat diet (HFD) group (C, Control), HFD with 1 g/kg b.w. of catechin group (PC, positive control), HFD with 600 mg/kg b.w. of T or P groups, and HFD with 150, 300, 600 mg/kg b.w. of TP groups. Feeding of HFD for 12 weeks resulted in significant increases in body weight compared to the ND group. FBF-TC groups showed reduced body weight, weight gain, mesenteric white adipose tissue (WAT) weight, subcutaneous WAT weight, serum total cholesterol, LDL-cholesterol, and triglyceride levels compared to the C group. In conclusion, this study suggests that TP is a novel anti-obesity candidate through its stimulation of lipolysis.

포도씨 추출물 등 복합물(FFM-GS)의 갱년기 증상 개선 효과

이민희(Minhee Lee),남다은(Da-Eun Nam),박수정(Soo-Jeung Park),김다경(Dakyung Kim),윤정문(Jeong-Moon Yun),김옥경(Ok-Kyung Kim) 한국식품영양과학회 2019 한국식품영양과학회지 Vol.48 No.3

본 연구에서는 여성 갱년기 개선 소재 개발을 위하여 포도씨, 발레리안, 홍화씨 추출물의 복합물인 ‘포도씨 추출물 등 복합물(FFM-GS)’의 in vitro 및 in vivo 효능을 평가하였다. In vivo로는 난소절제로 골다공증을 유도한 rat을 사용하여 6주간의 식이섭취를 통해 여성 갱년기 개선 효능 평가를 하였다. 6주간 FFM-GS를 섭취한 동물에서는 난소절제에 의한 체중 증가와 이상지질혈증(고콜레스테롤, 고 LDL 콜레스테롤, 고중성지방혈증) 증상이 완화되었음을 확인하였다. 또한 혈액 내 골 대사 지표인 ALP, osteocalcin을 측정한 결과에서는 FFM-GS 식이 섭취 후 감소하였으며, 골밀도와 골의 형태학적 계측을 측정한 결과에서는 감소한 골밀도, BV/TV, Tb.Th, Tb.N, Tb.Sp가 FFM-GS 식이 섭취로 인해 회복되었음을 확인할 수 있었다. 이러한 결과는 FFM-GS가 골 대사에 긍정적인 효과를 주었음을 시사할 수 있다. 세포를 이용하여 FFM-GS가 골 대사에 미치는 영향을 관찰한 결과에서 골 석회화 증가를 유도하고 ALP, osteocalcin, IGF-1, estrogen receptor의 활성 및 발현을 유의적으로 증가시켰음을 확인하였다. 따라서 본 연구의 결과에서는 FFM-GS가 여러 가지 biomarker의 조절 작용을 통해 여성 갱년기 개선에 긍정적인 영향을 미쳤음을 보여주었고, 이러한 효능은 건강기능식품의 개발을 위한 소재로 기초자료로 활용 가능할 것으로 보인다. This study examined the effects of grape (Vitis vinifera L.) seed, valerian (Valeriana officinalis), and safflower (Carthamus tinctorius) seed extract complex (grape seed extract complex, FFM-GS) using in vitro and in vivo models for the development of improved materials of women’s menopausal symptoms. Ovariectomized rats were used and fed either the control diet or diet supplemented with FFM-GS 100 and 300 mg/kg b.w. The weight gain and dyslipidemia (high cholesterol, high low-density lipoprotein cholesterol and hypertriglyceridemia) symptoms were alleviated in the ovariectomized rats. On the other hand, diet supplementation of FFM-GS decreased significantly the weight gain and dyslipidemia symptoms in ovariectomized rats. In addition, the serum alkaline phosphatase (ALP) and osteocalcin levels, and bone morphometric measurements markers [bone mineral density, percent bone volume (BV/TV), trabecular thickness (Tb.Th), trabecular number (Tb.N), and trabecular separation (Tb.Sp)] were recovered in ovariectomized rats supplemented with FFM-GS. The FFM-GS treatment increased the mineralization and mRNA expression levels of osteocalcin, ALP, IGF-1, and estrogen receptor in co-culture cells with MC3T3-E1 cells and femoral bone marrow from C57BL/6 mice. Therefore, FFM-GS has a potential positive effect on improving female postmenopausal symptoms, including weight gain, dyslipidemia, and osteoporosis.