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고추(Capsicum annuum)의 항균성 단백질(PR-5) 유전자의 클로닝과 발현 분석
박해진,이정훈,윤용휘,김학윤,신동현,이인중,김달웅,김길웅,Park, Hae-Jin,Lee, Jung-Hoon,Yoon, Yong-Hwi,Kim, Hak-Yoon,Shin, Dong-Hyun,Lee, In-Jung,Kim, Dal-Ung,Kim, Kil-Ung Korean Society of Life Science 2002 생명과학회지 Vol.12 No.3
식물은 병원균이나 여러 가지 환경스트레스에 내하여 사기 방어기작을 가지며, 특히 PR 단백질은 병원균의 침입시에 동물의 면역반응과 유사한 생체방어반응을 나타내는 중요한 역할을 하는 것으로 알려져 있다. 본 연구에서는 고추에서 항균 특성을 나타내는 PR5 유전자를 클로닝하고 이들의 특성을 구명하였다. 고추에서 서로 다른 3종의 PR5 유전자, CAPR5-1, CAPR5-2, CAPR5-3를 클로닝하였다. 이들 유전자의 특성을 조사하고 아미노산 수준에서 유사성을 비교하여 본 결과, 서로간에는 90% 이삳의 상동성을 나타내었고 이들의 2차구조를 비교한 결과 중요한 domain은 높은 상동성을 나타내어 PR5 유전자들이 항균 특성을 나타내는데 매우 중요한 motif로 작용할 것으로 사료된다. CAPR5-1, CAPR-2, CAPR5-3 유전자들의 항균성 정도를 조사하기 위하여 이들 유전자를 대장균에서 발현시켜 단백질을 분리하여 고추 역병균인 phytophthora capsici에 처리한 결과, 균사의 성장이 억제되어 CAPR5-1, CAPR5-2, CAPR5-3 단백질들이 항균성을 지니고 있는 것으로 나타났다. We have isolated and artificially expressed three cDNA clones of Capsicum annuum PR5 genes for elucidating the antifungal activity against Phytophthora capsici which contracted a hot pepper root rot in field condition. Three divergent PR5 proteins from hot pepper were designated as CAPR5-1 and CAPR5-2 from susceptible cultivar (Subicho) as well as CAPR5-3 from resistant cultivar (CM331) in response to P. capsici. The cDNA similarity was found over 80% of identity among the three CAPR5s, and deduced amino acid sequence was characterized that all of CAPR5s contained 16 cysteine residues which possibly had a significant role in the structural formation. The result of genomic DNA blot showed that CAPR5-1 and CAPR5-2 existed as single copy in the Subicho genome. Three recombinant CPARs in E. coli were identified by SDS-PACE, and each expressed protein was treated on the PDA medium which contained cultured pathogens. Although three CAPR5 proteins did not affected the hyphal growth of Glomerella glycines and Colletotrichum fagenarium, CAPR5-1, CAPR5-2, and CAPR5-3 showed a specific antifungal activities against P. capsici.
Young Kyung Lee(李螢京),Yong Hwi Yoon(尹用輝),Il Sun Chung(鄭鎰仙),Jong Soon Lee(李鍾焞),Sang Jong Lim(林尙鍾),In Gyu Song(宋仁圭),Dal Ung Kim(金達雄) 한국육종학회 1996 한국육종학회지 Vol.28 No.3
To analyse the genetic relationship among garlic accessions(Allium sativum L.) the polymerise chain reaction(PCR) was performed with total genomic DNAs of 13 garlic accessions by random 14 primers. The genetic diversity and genetic distance among 13 garlic accessions were used to generate a dendrogram showing phylogenic relationship. Thirteen garlic accessions were classified into two groups(group Ⅰ, Ⅱ). In additon, one of the two groups Group Ⅰ was divided into three sub-groups(group Ⅰ-a, Ⅰ-b, Ⅰ-c). Group Ⅰ included garlic accessions of Hongcheon, Gangwha, Yeongdong, Cheongju, Danyang, Gochang, Euesung, Yaechun, Seosan, Samcheog and China, while Group Ⅱ included garlic accessions of California and Austria. Sub-group Ⅰ-a included garlic accessions of Cheongju, Euesung, Yaechun, Seosan, sub-group Ⅰ-b included garlic accessions of Gangwha, Yeongdong, Samcheog and sub-group Ⅰ-c included garlic accessions of Hongcheon, Danyang, Gochang, China, respectively.
In Youl Baek(白寅烈),Yong Hwi Yoon(尹用輝),Dao Chull Shin(申斗澈),Gyu Hwan Park(朴圭煥),Young Hun Hwang(黃永鉉),Dal Ung Kim(金達雄) 한국육종학회 1997 한국육종학회지 Vol.29 No.3
Effective conservation and the use of plant genetic resources are essential for future agricultural progress. In this study, the genetic relationships among fourteen species of Glycine were analyzed based on the RAPDs. A total of fourteen species (twenty one accessions) in the genus Glycine were identified by the frequency of RAPDs polymorphisms. Ten primers out of thirty primers tested, were selected for the reliability and repeatability of banding patterns. The amplification of the genomic DNA produced eighty two polymorphic band patterns with the average of 8.2 bands per primer. By Nei and Li’s formula, the mean 1-F value (genetic similarity) was 0.461, and the highest value was 0.774 (G. max : G. microphylla) and the smallest one was 0.128 (intraspecies G. max). That of interspecies in the subgenus Glycine was 0.620 and 0.250 in the highest and the lowest cases, respectively. Thus the genetic similarities were much variable within the interspecies of subgenus Glycine but those were somewhat small between the subgenus Glycine and Soja. By UPGMA (unweighted pair group method using an arithmetic average) cluster analysis based on 1-F value, the fourteen species of Glycine could be classified into four major-groups (genetic similarity (GS) : 0.45) and nine sub-groups (GS : 0.35), that is, into Group Ⅰ : subgenus Soja : G. max, G. sofa, G. gracilis; Group Ⅱ : G. falcata ; Group Ⅲ : A : G. latrobeana, G. canescens, G. microphylla, B : G. curvata, G. cyrtoloba, C : G. clandestina, D : G. tabacina(2n=80) ; Group Ⅳ : A : G. arenaria, B : G. tomentella (2n=38, 40, 78, 80), C : G. tabacina (2n=40, 120), G. latifolia. By multidimensional scaling (MDS), the subgenus Glycine and Soja were clearly classified in the clustering of genetic relationship distance. The genetic relationship distance was closely identified between G. max of the cultigen and G. clandestina, G. tomentella, G. cyrtoloba, G. curvata of the subgenus Glycine.