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Plant Regeneration from Sliced Mature Embryo Fragments of Wheat Cultivars
김경희,오푸름,이병무,강문석,권영업,이상규,문준철,한시내 한국작물학회 2005 Korean journal of crop science Vol.50 No.6
Mature embryos were aseptically excised with a scalpel and sliced in fragments measuring 0.5 mm in diameter (sliced mature embryo fragment; 4~5 fragments/one embryo). Sliced mature embryo fragments of six wheat cultivars were cultured to develop an efficient method of callus induction and plant regeneration. Callus derived from sliced mature embryo fragments showed a good capacity to embryogenesis and regeneration. Furthermore sliced mature embryo fragments decreased contamination from fungi and bacteria. The high efficiency of callus induction were obtained Keumkangmil and Bobwhite. For plant regeneration, selected embryogenic calli were transferred to two types regeneration media. An average number of green spots per callus was 4 to 5 in regeneration media after about one week. Percentage of plant regeneration showed high in regeneration medium containing 0.1 mg/l 2,4-D and 5 mg/l zeatin. Especially, Keumkangmil (27.5%) and Bobwhite (33.3%) showed high regeneration efficiency. This regeneration system from sliced mature embryo fragments may provide an effective and convenient explant for plant transformation studies.
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송문영,원희재,김시형,이종우,오푸름,이형우,김혜린 한국보건사회약료경영학회 2020 한국보건사회약료경영학회지 Vol.8 No.2
BACKGROUNDS Community pharmacists contribute to safe and effective drug treatment for patients by per-forming various prescription interventions through simultaneous drug utilization review (DUR) and checking patient medi-cation history. Our study aimed to analyze the interventions on drug-related problems (DRPs) by pharmacist from a community pharmacy. METHODS We obtained data for DRPs and the interventions by pharmacist which was recorded between 2019.7.22 and 2019.8.30 in working hours on weekdays at a community pharmacy located in Nowon-gu. Data was collected using a well-developed form ("Pharmacist Intervention on DRPs"). Data contained patient related factors, charac-teristics of prescription issuing institutions, DRPs, pharmacist interventions, intervention acceptance, and time required for intervention recording. RESULTS Pharmacist' intervention occurred 47 cases (1.04%) among the total 3,831 prescriptions during the study period. Men and women were 50% each, and the average age was 58.3 (±20.6, SD) years. The most com-mon types of DRPs were 'duplication (same drug)' of 53.2% (25 cases), and as for the pharmacist intervention, 'discon-tinue medication' was done most frequently as 48.9% (23 cases). In the drug efficacy groups, the musculoskeletal drugs were the most common with 33.3% (16 cases), followed by the alimentary tract and metabolism drugs with 16.7% (8 cases). The average time spent on intervention recording was 4.1 minutes (±4.0). CONCLUSION The current status of DRP and pharmacist interventions was identified through data from one community pharmacy collected through a system-atic record format. Intervention by community pharmacists plays an important role in the safe and effective utilization of treatment.
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