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흰쥐 분리 간세포에 있어서 딜티아젬의 간클리어런스에 미치는 페노바르비탈의 영향
이용복,오준교,고익배,Lee, Yong-Bok,Oh, Joon-Kyo,Kho, Ik-Bae 한국약제학회 1996 Journal of Pharmaceutical Investigation Vol.26 No.1
In order to study the effect of phenobarbital(PB) on the hepatic transport of diltiazem(DTZ), $Ca^{2+}$ channel blocker, we used isolated hepatocytes of rat which was intraperitoneally pretreated with phenobarbital sodium(75 mg/kg) for four days once a day. For the isolation of rat liver cells, a modification of the two step procedure of Seglen was used. DTZ was dissolved in incubation buffer to the final DTZ concentrations of 200, 400, 600, 800 and 1000 ng/ml in order to elucidate the uptake characteristics of DTZ by hepatocytes. Reactions were stopped at 10, 20, 30, 45, 60, 90, 120 and 300 sec. The initial velocity was determined by disappearance of diltiazem in the hepatocyte suspension. On the other hand, to determine the effect of PB on the in vitro hepatic intrinsic clearance of DTZ we obtained the metabolism rates of DTZ in the control and the PB-pretreated rat hepatocyte at various time intervals. According to pretreatment with PB, the size of hepatocyte and the amount of protein per $10^6$ cells were significantly (p<0.01) increased from $26.92{\pm}0.1364\;m$ to $35.31{\pm}1.00\;m$ and from $468{\pm}6.5\;{\mu}g/10^6$ cells to $628.8{\pm}12.1{\mu}g/10^6$ cells, respectively. In the case or hepatic uptake of diltiazem, $K_m$ was not different in the normalization by cell numbers and increased from $2.90\;{\mu}M\;to\;13.89\;{\mu}M$ in the normalization by protein amount. $V_max$ was increased regardless of normalization by protein amount and cell numbers, from $1.21\;{\mu}mole/min \;{\cdot}\;mg\;protein\;to\;3.96\;{\mu}mole/min\;{\cdot}\;mg\;protein\;and\;from\;2.38\;{\mu}mole/min\;{\cdot}\;10^6\;cells\;to\;2.83\;{\mu}mole/min\;{\cdot}\;10^6\;cells$, respectively. The in vitro hepatic intrinsic clearance of DTZ was significantly (p<0.01) increased from $0.640{\pm}0.038\;ml/mim\;{\cdot}\;10^6\;cells\;to\;2.385{\pm}0.212\;ml/min\;{\cdot}\;10^6\;cells$ due to PB-pretreatment. These results suggest that the uptake of DTZ by hepatocyte is extremely fast and PB enhances the hepatic intrinsic metabolic clearance of DTZ.
흰쥐 분리 간세포에 있어서 딜티아젬의 간클리어런스에 미치는 페노바르비탈의 영향
고익배,이용복,오준교 한국약제학회 1996 Journal of Pharmaceutical Investigation Vol.26 No.1
In order to study the effect of phenobarbital(PB) on the hepatic transport of diltiazem(DTZ), Ca^(2+) channel blocker, we used isolated hepatocytes of rat which was intraperitoneally pretreated with phenobarbital sodium(75㎎/㎏) for four days once a day. For the isolation of rat liver cells, a modification of the two step procedure of Seglen was used. DTZ was dissolved in incubation buffer to the final DTZ concentrations of 200, 400, 600, 800 and 1000 ng/㎖ in order to elucidate the uptake characteristics of DTZ by hepatocytes. Reactions were stopped at 10, 20, 30, 45, 60, 90, 120 and 300 sec. The initial velocity was determined by disappearance of diltiazem in the hepatocyte suspension. On the other hand, to determine the effect of PB on the in vitro hepatic intrinsic clearance of DTZ we obtained the metabolism rates of DTZ in the control and the PB-pretreated rat hepatocyte at various time intervals. According to pretreatment with PB, the size of hepatocyte and the amount of protein per 10^6 cells were significantly (p<0.01) increased from 26.92±0.1364 m to 35.31±1.00 m and from 468±6.5 ㎍/10^6 cells to 628.8±12.1 ㎍/10^6 cells, respectively. In the case of hepatic uptake of diltiazem. K_m was not different in the normalization by cell numbers and increased from 2.90 μM to 13.89 μM in the normalization by protein amount. V_(max) was increased regardless of normalization by protein amount and cell numbers, from 1.21μmole/min·㎎ protein to 3.96μmole/min·㎎ protein and from 2.38 μmole/min·10^6 cells to 2.83μmole/min 10^6 cells, respectively. The in vitro hepatic intrinsic clearance of DTZ was significantly (p<0.01) increased from 0.640±0.038 ㎖/mim·10^6 cells to 2.385±0.212 ㎖/min·10^6 cells due to PB-pretreatment. These results suggest that the uptake of DTZ by hepatocyte is extremely fast and PB enhances the hepatic intrinsic metabolic clearance of DTZ.
최민석(Min-Seok Choi),최찬욱(Chan-Wook Choi),송선호(Sun-Ho Song),오준교(Jun-Kyo Oh),한영오(Young-Oh Han) 한국전자통신학회 2024 한국전자통신학회 논문지 Vol.19 No.1
본 논문은 반려동물의 비만율이 증가하는 현대사회에서 애완동물의 비만 관리를 위해 사료 배식과 운동량 측정 등의 기능을 수행하는 앱 기반 급식기와 활동량 측정 웨어러블 목걸이를 제안한다. 웨어러블 목걸이는 애완동물의 활동량을 측정하고 하루 평균 활동량에 따라 적절한 사료량을 제안한다. 급식기는 예상치 못한 상황에서도 비만 관리가 가능하게 하루 적정 사료량을 앱을 통하여 제공할 수 있는 앱 기반 애완동물 급식 시스템을 연구하였다. This paper proposes an app-based feeder that performs functions such as distributing food and measuring exercise amount and a wearable collar that measures activity level to manage pet obesity in modern society where the obesity rate of pets is increasing. The wearable collar measures the pet’s activity level and suggests the appropriate amount of food based on the average daily activity level. We studied an app-based pet feeding system that can provide the appropriate amount of feed per day through an app to enable obesity management even in unexpected situations.