실내수조에서 인위적 수온자극이 참가리비 폐사에 미치는 영향

오봉세,조규태,이정용,권문경,이주 한국패류학회 2011 The Korean Journal of Malacology Vol.27 No.3

We examined the effect of an artificial change of water temperature on the survival rate of Korean Scallop, Patinopecten yessoensis. As for the change of water temperature, the aspects appearing on the eastern coast of Korea during spring and summer were artificially prepared in the rearing aquarium in the laboratory. The survival rate of Scallop, due to the stimulus of a low water temperature in spring, was an average of 51.6%. Medium-sized scallops averaged 4.4%, which is lower than that of small-sized ones (85.82%) and large-sized ones (55.0%). The survival rate of Scallop, due to the stimulus of water at a high temperature in summer, was an average of 43.3%. As for small-sized scallops, they averaged a 46.7% survival rate which is lower than that of medium-sized scallops (60.0%). In general, the survival rate was lower in summer than in spring. Such results show that the death of Scallop, has close relevance to the change of water temperature. The low survival rate of medium-sized ones. Alternatively, medium-sized scallops have to get through winter, even if they cannot grow enough compared with large-sized ones. This is because large-sized scallops have already been adapted to the change of water temperature by getting through winter twice. 인위적 수온급변이 참가리비의 생존율에 미치는 영향을 조사하였다. 수온변화는 봄과 여름철에 동해안에서 나타나는 변화양상을 실험실 내 사육수조에서 인위적으로 유도하였다. 봄철 저수온 기간 중 수온 자극에 의한 참가리비의 생존율은 평균 51.6%를 보였고, 소형패 (85.82%) 와 대형패 (55.0%) 보다 중형패에서 4.4%로 가장 낮았다. 여름철 고수온 기간 중수온 자극에 의한 참가리비의 생존율은 평균 43.3%를 보였으며, 중형패 (60.0%) 보다 소형패가 46.7%로 낮게 나타났다. 대체적으로 봄철보다 여름철에 생존율이 더 낮았다. 이와 같은결과들은 참가리비의 폐사가 수온 변화와 밀접한 관련이 있음을 보여주고 있다. 봄철 중형패의 낮은 생존율은 충분한 영영공급을 받는 소형패나 두 번의 겨울을 보내 수온변화에 적응한대형패에 비해 충분히 성장하지 못한 상태에서 겨울을 보내기때문에 나타나는 결과로 보인다.

중국산 참가리비, Patinopecten yessoensis의 인공종묘 생산 및 부착치패 중간양성에 관한 연구

오봉세,이정용,박세규,이주,조규태 한국패류학회 2008 The Korean Journal of Malacology Vol.24 No.2

We investigated artificial mass seed production of a Chinese scallop, Patinopecten yessoensis, in 2004. The GSI (gonad somatic index) of the Chinese scallop, P. yessoensis was 17.2 on mid-February, 20.2 on mid-March, while that of Korean scallop, P. yessoensis was 6.9 on mid-February, 10.8 on mid-March. Matured 120 females and 350 males were selected for artificial mass production. They were exposed in air for 1 hr at over 20℃, and placed into a spawning tank (20 ton) containing sea water treated with UV radiation at 12℃. We gained a total of 228,000 thousand scallop embryos between March 10th and 15th, and reared larvae at the indoor tank during 25 days. When the mean shell length of larvae reached 250 ㎛ and they have eye-spots, the number of pre-settling larvae was 47,500 thousand. We gained 1,850 thousand attached scallop spats from two kinds of collectors. Attached spats were reared in indoor tank for different periods from 5 days to 60 days. They were divided into 5 groups according to the length of reared days. Each group of attached spats was moved to intermediate rearing sites at Yangyang fishing port in Gangreung-city for acclimation to ocean environments. The highest survival rate of attached spats was 13.0% shown at the group reared for 12 days, but the significant difference in their growth was not found between the groups. The shell length of artificial attached spats increased from 0.9 ㎛ on July 10th to 24.7 ㎛ on December 16th with the survival rate of 85.0% while that of natural attached spats increased from 0.6 ㎛ on July 10th to 23.9 ㎛ on December 16th with the survival rate of 85.7%. We investigated artificial mass seed production of a Chinese scallop, Patinopecten yessoensis, in 2004. The GSI (gonad somatic index) of the Chinese scallop, P. yessoensis was 17.2 on mid-February, 20.2 on mid-March, while that of Korean scallop, P. yessoensis was 6.9 on mid-February, 10.8 on mid-March. Matured 120 females and 350 males were selected for artificial mass production. They were exposed in air for 1 hr at over 20℃, and placed into a spawning tank (20 ton) containing sea water treated with UV radiation at 12℃. We gained a total of 228,000 thousand scallop embryos between March 10th and 15th, and reared larvae at the indoor tank during 25 days. When the mean shell length of larvae reached 250 ㎛ and they have eye-spots, the number of pre-settling larvae was 47,500 thousand. We gained 1,850 thousand attached scallop spats from two kinds of collectors. Attached spats were reared in indoor tank for different periods from 5 days to 60 days. They were divided into 5 groups according to the length of reared days. Each group of attached spats was moved to intermediate rearing sites at Yangyang fishing port in Gangreung-city for acclimation to ocean environments. The highest survival rate of attached spats was 13.0% shown at the group reared for 12 days, but the significant difference in their growth was not found between the groups. The shell length of artificial attached spats increased from 0.9 ㎛ on July 10th to 24.7 ㎛ on December 16th with the survival rate of 85.0% while that of natural attached spats increased from 0.6 ㎛ on July 10th to 23.9 ㎛ on December 16th with the survival rate of 85.7%.

A Study on Survival and Growth of Ark shell, Scapharca broughtonii with differential density during hanging culture

오봉세,진영국,정춘구,임월애,김숙양 한국패류학회 2014 The Korean Journal of Malacology Vol.30 No.1

Ark shell Scapharca broughtonii length and total weight growth by density, which was hanging-cultured near the sea area of Yeosu-city, Hoenggan-island (below is Yeosu), showed inverse correlation with density. As for the average growth of shell length by density, the test area of 10 shells showed the best result as 5.21 mm, and it became lower as density became higher. Also, the increase of total weight by density showed the same result, thus on the whole, the increase of average shell length and total weight of 10-20 shell test area showed the better result than the test area of 30-40 shells. In addition, even if the parashell’s shell length by density and total weight growth in the sea area of Namhae-gun, Mijo-fishing port (below is Namhae) showed inverse correlation with density, it showed the slighter inverse correlation than the sea area of Yeosu. As for the average growth of shell length by density, the test area of 10 shells showed the best result as 4.55mm, but the average growth of shell length by density in the test area of 20-40 shells appeared to be similar as 3.11, 3.36 and 3.27 mm. On the other hand, as for the increase of total weight by density, the test area of 10-20 shells showed the more increase than the test area of 30-40 shells. In conclusion, it is guessed that the culture density of 20 shells/0.12 m2 (166 shells / 1 m2) would be appropriate when hanging-culturing the shells. At the sea area of A, the survival rate of Scapharca broughtonii parashell was 68-87% by density respectively, and it showed the density-dependent result, while the survival rate of sea area of Namhae, where phytoplankton as prey is abundant, showed the much better result as 91-100%. It is guessed that this may be due to the fact that the quantity of phytoplankton in sea area of Yeosu is just 55.89% of sea area of Namhae. When hanging-culturing the shells, the water temperature by sea area ranged from 16.22 to 25.66℃, salinity 30.07-33.48, pH 8.10-8.45, and DO 4.49-9.65 mg/L. 여수 횡간도 지역에서 밀도별 피조개 각장 과 전중량의 성장은 밀도와 역 상관관계를 보였다. 밀도별 각장 평균성장은10마리구가 5.21 mm로 가장 양호하였으며 밀도가 높을수록각장 성장은 낮아졌다. 밀도별 전중량의 증가도 같은 결과를보여 전반적으로 10-20마리구의 평균각장 및 평균 전중량의증가가 30-40마리구에 비하여 양호하게 나타났다. 남해해역에서 밀도별 피조개 각장과 전중량의 성장도 밀도와 역 상관관계를 보였으나 여수해역보다 약한 역 상관관계를보였다. 밀도별 각장 평균성장은 10마리구가 4.55 mm로 가장 양호한 결과를 보였으나, 20-40마리구의 밀도별 평균각장의 성장은 3.11, 3.36, 3.27 mm로 비슷하게 나타났다. 반면에 밀도별 전중량의 증가는 10-20마리구가 30-40마리구보다많았다. 결론적으로 피조개의 수하양성시 양성밀도는 20마리/0.12 m2 (166마리/1 m2) 가 적당하다고 사료되었다. 여수해역에서 피조개의 생존율은 밀도별로 각각 68%-87%, 로 밀도의존적 결과를 보였으나, 먹이생물인 식물플랑크톤이풍부한 남해해역의 생존율은 91%-100%로 월등히 양호한 결과를 보였는데, 이는 여수해역의 식물플랑크톤의 양이 남해해역의 55.89%에 불과하였기 때문이라 사료된다. 수하 양성 해역별의 수온범위는 16.22-25.66℃였고, 염분은30.07-33.48였으며, pH는 8.10-8.45범위였다. 그리고 용존산소 (DO) 는 4.49-9.65 mg/L 였다.

The Effect of Yellow Soil on Mortality of Korean Scallops, Patinopecten yessoensis at Indoor Tank

오봉세,정춘구,권문경,이정식 한국패류학회 2010 The Korean Journal of Malacology Vol.26 No.3

In other to understand the effect of yellow soil to mortality of Korean scallops, P. yessoensis, We investigated its mortality at indoor tanks. The environmental conditions such as water temperature, Salinity, Do and pH were continued constantly during the experimental periods. The 100% of survival rate showed in two experiments groups such as 0.1% and 0.4% of concentration of yellow soil and the other groups as 0.05% and 0.2% of concentration of yellow soil was appeared one dead scallop at each group for 8 days of the experiment periods. the gills of scallop in high concentration of yellow soil (0.2% and 0.4% groups) were covered by yellow soil particles so that this group's scallop should be got a high stress from yellow soil. I think this situation will be more continued for long time the scallop will become to dead. The results of bacteriological analysis did not isolated from haemolymph of scallops and no Perkinsus infectious disease in scallops and the scallops showed necrosis and degeneration on digestive grand and gills of scallop.

벵에돔 Girella punctata와 긴꼬리 벵에돔 Girella melanichthys의 난 발생에 미치는 수온의 영향

오봉세,구학동,김성철,정민민,박흥식,최영웅 한국발생생물학회 2010 발생과 생식 Vol.14 No.2

This research investigated that the effect of water temperature on egg developmental stages of largescale blackfish Girella punctata and smallscale blackfish Girella melanichthys. The required times from fertilized embryos to hatching for the G. punctata were 67.8~37.5 hrs from 15 to 21℃, shorter as the water temperature increased. But fertilized embryos were dead after Kuffer's vesicle appearance stage at 24℃ and morula stage at 27℃. Biological minimum temperature (starting point for embryonic development) of the egg development was estimated to be 7.6℃ in average. In G. melanichthys, fertilized embryos were hatched within 61.2~38.3 hr from 15 to 21℃, the times were shorter as the water temperature increased to hatching, but its were dead on above 24℃. Biological minimum temperature of the egg development was estimated to be 6.5℃ in average. Based upon these results, it is recommended that the range of optimum water temperature for embryonic development of G. punctata and G. melanichthys is 15~21℃.

참게류의 양식에 관한 연구 I. 동남참게, Eriocheir japonicus 의 야외사육지별 성장효과

오봉세,김숙양 한국양식학회 1998 韓國養殖學會誌 Vol.11 No.2

The results of experimental culture for Koran mitten crab, Eriocheir japonicus at different outdoor culture methods such as rice field, pond, and vinyl house from July 21, 1994 to October 23, 1995 were summaried as fellows. 1. The water temperature was 2${\circ}C$~32${\circ}C$ during the culture periods from July 21, 1994 to October 23, 1995, and it ranged from 15${\circ}C$ to 30${\circ}C$ during the terms showing the intake habit of feed with these temperature maintained for 281 days of 460days of the total cultur periods. 2. The gain weight of Korean mitten crabs was 1,711.3g at the rice field, and 1,565.4g at the pond, and 1.220.0g at the vinyl house. The survial rate was 22.7% at the pond, and 18.7% at the rice field, and 18.3% at the vinyl house with the average survial rate of 19.9%. The daily growth rate of cauapace width was 0.64% at the rice field, and 0.61% at the pond, and 0.59% at the vinyl house. And the gain weight and the daily growth rate were the best at the rice field. 3. When the culture was finished, the frequency of carapace width of 4.0~4.9cm was 57.1% at the rice field, and 33.3% at the vinyl house. Therefore, culture at the rice field for the cultur of mitten card was considered to be the best outdoor culture method from the point of view in the fast growth and polyculture feasibility with paddy rice. 1994년 7월 21일부터 1995년 10월 23일까지 논, 지수, 비닐하우스 야외사육지를 이용하여 동남참게를 사육한 결과, 1. 사육기간중 수온은 $1.5{\circ}C$~34.2${\circ}C$이었으나, 먹이를 정상적으로 먹은 기간의 수온은 15${\circ}C$~30${\circ}C$로 나타났으며, 이는 총 460일의 사육기간 중 281일(61.1%)에 해당되었다. 2. 증중량은 논(1,711.3 g), 지수(1,565.4 g), 비닐하우스(1,220.0 g)사육지의 순이었고, 생존율은 지수922.7%), 논(18.7%), 비닐하우스(18.3%)사육지의 순이었으며, 갑폭의 일간성장률은 논(0.61%), 지수(0.60%), 비닐하우스(0.59%)사육지의 순으로 논 사육지의 증중량 및 일간성장율이 타 사육지보다 다소 양호하였다. 3. 사육종료시 사육지별 갑폭의 출현모드를 보면 지수 및 비닐하우스 사육지의 모드는 3 cm군으로 나타난데 비하여 논 사육지는 모드가 4 cm군으로 나타났을 뿐 아니라, 증줄량과 일간성장율도 다소 높고 벼 경작도 병행할 수 있어 타 야외사육지보다 동남참게 양식에 적합한 사육지로 사료되었다. 4. 동남참게 내장의 일반성분을 분석한 결과 양식산이 자연산에 비하여 조지방 함량이 다소적게 나타난 것을 제외하고는 영양성분상의 큰 차이는 없었다.

해만가리비, Argopecten irradians의 인공산란과 유생 및 치패의 발생

오봉세,정춘구,김숙양 한국패류학회 2003 The Korean Journal of Malacology Vol.19 No.1

Artificial spawning, larval and spat developments of the bay scallop, Argopecten irradians, which was transplanted from China on 16 August 1996, were investigated monthly until August 1997 in the Deukyang Bay, Jangheung-gun, Jeollanam-do, Korea. Sufficient amount of cultured microalgae supplemented seawater were supplied as food (6 kinds of phytoplankton) for fully grown adult individuals at 17.1-23.2℃ for 44 days. A total of 45,320,000 eggs were spawned by way of 2 times of artificial spawning inductions such as exposure stimulus to the air and thermal shock (with water temperature) on 29 January and 31 January in 1997. Artificially fertilized eggs were developed to D-shaped larvae (77.5 × 63.8 μm) and metamorphosed to larvae (191.8 × 181.2 μm) in the attached larval stage on the collectors. A total of 110,000 spats (average 3.04 mm in shell length) were produced at 22.8-26.3℃ and 31.0-34.4 psu in the indoor rearing tank from 14 February through 7 May in 1997. In case of Argopecten irradians, if the attached larvae in the attachment stage are detached from the collector, they could not live. Accordingly, it is assumed that survival (%) of the attached larvae of A. irridians showed very low because of weak power attached to the collector due to the small number of the byssuses of the attached larva, not the short attachment period by the byssus as seen in other scallops such as Argopecten balloti. 중국에서 이식하여 어미패로 양성한 해만가리비를 수온 17.1-23.2℃로 44일간 Isochrysis galbana 등 5종의 먹이생물을 혼합시켜 공급하면서 성숙을 유도하여, 1997년 1월 29일 및 31일 2회에 걸쳐 간출과 수온 자극을 가하여 총 4,532만개의 수정란 (평균 52 μm) 을 채란하였다. 난 발생 단계를 조사한 결과, D형 유생의 크기는 평균 77.5 × 63.8 μm이었고, 부착기 유생은 191.8 × 181.2 μm의 크기였으며, 부착기 유생은 이 크기에서 파판에 부착하여 치패로 발달하였다. 이 치패는 2월 14일부터 5월 7일까지 수온 22.8-26.3℃, 염분 31.0-34.4에서 실내 사육한 결과, 평균 각장 3.04 mm로 성장하였다. 비단가리비의 경우는, 부착기의 부착유생들은 채묘기(collector) 에서 떨어지면 폐사하게 되는데, 이것은 Argopecten balloti에서 나타나는 바와 같이, 족사에 의한 부착기간이 짧아서가 아니라 부착유생들이 각기 소수의 족사를 가지기 때문에 채묘기에 대한 부착력이 약하여 부착유생의 생존율이 비교적 낮았던 것으로 사료된다.

한국 남해안에 이식한 중국산 해만가리비, Argopecten irradians의 동절기 성장에 관한 연구

오봉세,정춘구 한국패류학회 1999 The Korean Journal of Malacology Vol.15 No.2

To develop new products for aquaculture, which fishermen at South Sea can get high income, after bay scallop was transplanted from Sandong province in China from October 17, 1996 to March 18, 1997 at Duekrang Bay in Janghung gun and Kamak Bay in Y대녀 City on Chollanam province, Sumjin river estuary in Hadong gun and Gejae Bay in Keoje city on Kyongsangnam proince. Temperature was decreased from 20.5$^{\circ}C$ to 5.1$^{\circ}C$ in February and then it was increased to 12.0 in March. Ranges of salinity, DO and Chlorophyll-a were 22.40-34.70 psu, 5.24-9.77 mg/l and 1.27-22.28 $\mu\textrm{g}$/l, respectively, and low temperature, sudden changes of salinity and low concentration of chlorophyll-a influenced the growth of bay scallop. bay scallop in the rearing period showed the fastest growth from October 17 to November 18 through the every size rages, while it grew slowly or stopped growing from December 18 to March 18. Among 4 transplanted areas, young scallop at Duekrang Bay showed the fastest growth and then Kamak Bay, Gejae Bay, Sumjin estuary in order. The daily growth rate of young scallop in the middle size range (5.70%) was higher than those in large (2.07%) and small (5.49%) size range. The meat weight index showed the highest in December. Survival rates from October to December were high, not related to the size ranges, but survival rate of the small young scallop from January to March was higher than Those of the rest. As the Result, transplanted bay scallop at South Sea was shown to grow by the middle of December (around 1$0^{\circ}C$ temperature).

팔당호 어업대상 어류의 종조성과 계절변동

오봉세,류재근,남명모,김숙양 한국수생태학회(구 한국하천호수학회) 1999 생태와 환경 Vol.32 No.4

해만가리비, Argopecten irradians의 해역별 성장

오봉세,양문호,정춘구 한국패류학회 2003 The Korean Journal of Malacology Vol.19 No.2

Water temperature during the culturing period was 10.4-25.5℃ and there was a little difference between rearing sites. Salinity (25.00-31.17 psu) and DO (over 6.13 mg/l) showed reasonable condition for the growth of bay scallop. Concentrations of chlorophyll-a were ranged 1.69-7.40 μg/l, and they fluctuated monthly in the every sampling site. During the growing period from June to October, dominant phytoplankton species were Ceratium sp. in Nammeon and Hoejin, Chaetoceros sp. in Dolsan. Density of phytoplankton was high in July, August and October, but it was low in September and November.When young bay scallops were cultured for 185 days in Nammyeon, Hoejin and Dolsan, shell heights were grown for 0.19 mm/day, 0.18 mm/day and 0.16 mm/day, respectively, and total weights were increased 0.16 g/day, 0.16 g/day and 0.13 g/day, respectively. Daily growth rates of shell height were 0.606%, 0.581% and 0.549%, and daily growth rates of total weight were 1.972%, 1.857% and 1.746%, respectively. Maximum predicted values of shell height calculated by von Bertalanffy growth model were 52.62 mm, 51.74 mm and 48.91 mm, respectively. Survival rate was the highest in Nammyeon (87.0%), but the rates in Hoejin and Dolsan sharply decreased after November. 우리나라 남해안의 남면, 회진 및 돌산의 3개 해역에서 해만가리비의 사육기간 중 수온은 10.4-25.5℃로 해역별 수온차이는 크지 않았으며, 염분은 25.00-31.17 psu, 용존산소는 최저 6.13 mg/l 이상으로 해만가리비 성장에 적합한 범위였다. Chlorophyll-a는 1.69-7.40 μg/l로 낮은 농도를 보인 시기도 있었다. 6-8월 해만가리비 성장기에 phytoplankton 우점종은 성장이 좋았던 남면에서는 편모조류인 Ceratium sp.가 35.2%, 회진에서는 Ceratium sp.가 25.5% 출현하였으나, 성장이 낮았던 돌산에서는 규조류인 Chaetoceros sp.가 40.4%로 우점하였다. 플랑크톤의 밀도는 대체적으로 8-9월에 높았고, 10월에 낮았다.해만가리비의 치패를 대상으로 185일간의 사육한 결과, 각고는 남면 0.19 mm/day, 회진 0.18 mm/day, 돌산 0.16 mm/day였고, 전중량은 남면 0.16 g/day, 회진 0.16 g/day, 돌산 0.13 g/day였다. 각고의 일간성장률은 남면이 0.606%, 회진 0.581%, 돌산 0.549%였고, 전중량의 일간성장률은 남면에서는 1.972%, 회진에서는 1.857%, 돌산에서는 1.746%였다. von Bertalanffy 성장모델에 의해 얻어진 각고의 최대 예상값은 각각 52.62 mm (남면), 51.74 mm (회진), 48.91 mm (돌산)였다. 생존율은 남면이 87.0%로 가장 높았으나, 회진 및 돌산은 11월 이후 급감하였다.