울금(Curcuma longa L.)의 생리활성 및 지질과산화 저해능에 미치는 영향

오다영,김한수,Oh, Da-Young,Kim, Han-Soo 한국응용과학기술학회 2019 한국응용과학기술학회지 Vol.36 No.2

울금(Curcuma longa L.)의 생리활성 및 지질과산화 저해능에 미치는 영향을 확인하고 기능성 식품 소재로 활용 가치를 검토하기 위하여 연구를 수행하였다. 총 카로티노이드(total carotenoid) 함량은 $1.581{\pm}0.005mg$ ${\beta}$-carotene equivalents (BCE)/g dry weight으로 관찰되었다. 70% 메탄올, chloroform:methanol (CM, 2:1, v/v), 에틸 아세테이트(ethyl acetate, EA) 3가지 용매를 사용한 추출 수율은 70% 메탄올(16.54%), CM (5.64%), EA (4.14%) 순으로 나타났다. 총 페놀 함량은 EA, CM 및 70% 메탄올에서 각각 106.287, 90.614 및 18.527 mg gallic acid equivalents (GAE)/g의 함량으로 나타났으며, 추출 용매 별 항산화능은 0.2, 0.4, 0.6, 0.8 mg/mL의 농도로 측정하였고, 양성대조구로 사용된 BHA (butylated hydroxyanisole) 및 trolox 보다는 낮은 항산화능을 보였다. Nitric oxide (NO) 라디칼 소거능은 70% 메탄올에서 28.65~48.43%, CM 18.86~55.10%, EA에서 15.68~56.25%로 관찰되었다. Nitrite ($NO_2$) 소거능은 70% 메탄올, CM 및 EA 순으로 나타나 EA 추출물에서 유의적인 차이를 보이며 강한 $NO_2$ 소거능을 나타내었다(p<0.05). ${\beta}$-carotene 탈색 저해능은 70% 메탄올, CM 및 EA에서 각각 1.64~23.79%, 6.99~41.16% 및 10.20~48.52%로 관찰되었다. 한편, 지질과산화 저해능은 70% 메탄올, CM 및 EA 추출물에서 높게 측정되었다. The aim was to determine the physiological activity and antioxidant activity by lipid peroxidation inhibitory action of turmeric (Curcuma longa L.). Bioactive compound of total carotenoid $1.581{\pm}0.005mg$ ${\beta}$-carotene equivalents (BCE)/g dry weight. Total phenol content was the highest in the ethyl acetate (EA) extract, followed by chloroform:methanol (CM, 2:1, v/v) and 70% methanol extracts. Antioxidant effects (nitrogen oxide radical scavenging activity, nitrite scavenging activity, ${\beta}$-carotene bleaching assay, and lipid peroxidation inhibition action) of 70% methanol, CM, and EA extract of turmeric. Turmeric extracts yield were 70% methanol 16.54%, CM 5.64%, and EA 4.14%, respectively. Antioxidant activity of the samples exhibited a dose-dependent increase. However, in the current study, none of the samples evaluated showed activity as strong as the BHA (butylated hydroxyanisole) and trolox. Further, nitrite scavenging activity was the highest for the EA extract. As a result of this experiment, indicating their commercial value and potential applications in food and nutraceuticals.

남빙양 krill이 흰쥐의 혈청 지질성분 및 알부민/글로불린 비에 미치는 영향

오다영 ( Da-young Oh ),진동혁 ( Dong-hyeok Jin ),강동수 ( Dong-soo Kang ),이영근 ( Young-geun Lee ),김한수 ( Han-soo Kim ) 한국환경과학회 2018 한국환경과학회지 Vol.27 No.11

The aim of the study were to investigate the influences of krill (Euphausia superba) meal on the body weight, lipid metabolism functional improvement, blood glucose level, protein component in the sera of rats which fed experimental diets for 5 weeks. Serum concentrations of total cholesterol, Low-Density Lipoprotein (LDL)-cholesterol, free cholesterol, triglyceride (TG), phospholipid (PL) and blood glucose were higher in the control diet group (G1 group) than the control diet plus 10% krill meal group (G2 group), the control diet plus 20% krill meal group (G3 group), the control diet plus 30% krill meal group (G4 group), and a general dose and time independent one-way analysis of variance was performed to assess efficacy. Conversely depending on the content of krill meal for the High-Density Lipoprotein (HDL)-cholesterol level, it showed higher results. The concentrations of total protein, albumin and globulin in sera, there were not significant difference among the groups (p<0.05). The results indicate that a krill meal diet effectively inhibited increases in lipid elevation, blood glucose level in the sera of rats.

진도산 울금(Curcuma longa L.)의 이화학적 특성

오다영 ( Da-young Oh ),김한수 ( Han-soo Kim ) 한국환경과학회 2019 한국환경과학회지 Vol.28 No.4

Studies on the physicochemical properties of physiological activity substance in turmeric (Curcuma longa L.) were analyzed for the use as an functional food materialization. The proximate compositions in the vacuum freeze dried turmeric were carbohydrate 72.90%, moisture 5.74%, crude protein 10.02%, crude fat 4.67%, and crude ash 6.69%, respectively. The mineral contents of turmeric were calcium (Ca) 2,294.77 mg kg^-1, potassium (K) 28,780.54 mg kg^-1, magnesium (Mg) 2,826.90 mg kg^-1, sodium (Na) 1,826.58 mg kg^-1, iron (Fe) 190.94 mg kg^-1, and manganese (Mn) 620.16 mg kg^-1. The vitamin contents of turmeric were pantothenic acid 1.040 mg/100 g, riboflavin 0.166 mg/100 g, thiamin 0.148 mg/100 g, pyridoxine 0.010 mg/100 g, and calciferol 0.008 mg/100 g, respectively. Total amino acid contents in protein of turmeric were 7.66 g%, and major amino acids were aspartic acid 1.45 g%, glutamic acid 1.07 g%, leucine 0.71 g%, phenylalanine 0.47 g%, and arginine 0.46 g%, respectively. The amount of free amino acids of turmeric were 225.81 mg%, and major free amino acids were asparagine, aspartic acid, glutamic acid, serine, and alanine. Especially, in the case of asparagine, it was highest. The compositions of fatty acid were saturated fatty acid 45.09%, monoenes 8.62%, and polyenes 46.30%.

진도산 울금(Curcuma longa L.) 추출물의 총 플라보노이드 함량 및 항산화 활성

오다영 ( Da-young Oh ),김한수 ( Han-soo Kim ) 한국환경과학회 2019 한국환경과학회지 Vol.28 No.4

The present study were conducted to determine physiological activities and antioxidant effects [2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging activity, reducing power, Ferric Reducing Antioxidant Power (FRAP) and Fe2+ (ferrous ion) chelating capacity] of 70% methanol, chloroform:methanol, 2:1 volume ratio (CM) and ethyl acetate extract of turmeric (Curcuma longa L.). Bioactive compound of tannin 0.125±0.007 mg Catechin Equivalent (CE)/g dry weight. Turmeric extracts yield were 70% methanol 16.54%, CM 5.64% and ethyl acetate 4.14%, respectively. Antioxidant activity of the samples exhibited a dose-dependent increase. Results showed that extraction solvent had significant effects on total flavonoid content and antioxidant effects of ethyl acetate. But ferrous ion-chelating capacity of 70% methanol extract was higher than CM and ethyl acetate extract. From the results of this study, turmeric can be utilized as a valuable and potential nutraceutical for the functional food industry.

코팅제를 이용한 인공경량골재의 흡수율 제어에 관한 연구

오다영(Oh, Da Young),최지선(Choi, Ji Sun),최훈제(Choi, Hoon Jae),이근주(Lee, Kewn Chu),하주형(Ha, Ju Hyung),조윤구(Cho, Yun Gu) 한국콘크리트학회 2016 한국콘크리트학회 학술대회 논문집 Vol.28 No.1

울금(Curcuma longa L.)이 이상지질혈증 흰쥐의 혈액요소질소(BUN) 및 효소활성에 미치는 영향

오다영 ( Da-young Oh ),강동수 ( Dong-soo Kang ),이영근 ( Young-geun Lee ),김한수 ( Han-soo Kim ) 한국환경과학회 2019 한국환경과학회지 Vol.28 No.5

The purpose this study was to investigate the influences of 5% turmeric (Curcuma longa L.) supplementation on enzyme activities such as aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), amylase, lipase and catalase in serum of dyslipidemic rats. Sprague-Dawley (SD) rats (24 male) were divided into four groups, namely the ND group (normal-nondyslipidemic diet), NT group (normal-nondyslipidemic diet+5% turmeric), DD group (control-dyslipidemic diet), and DT groups (dyslipidemic diet+5% turmeric). Serum concentrations of blood urea nitrogen (BUN), creatinine and uric acid were significantly decreased (p<0.05) by turmeric supplementation diet. The activities of AST, ALT, ALP, LDH, amylase and lipase in sera of turmeric diet group were significantly decreased (p<0.05). The catalase activity in serum of turmeric supplementation group was significantly increased than dyslipidemic diet (p<0.05). In vivo experiment with dyslipidemic rats showed that ingestion of turmeric were effective in kidney and hepatic functional enzyme activities. Which suggests that turmeric material could be used for further studies as a potential source for nutraceutical foods.

목구조 접합부 적용을 위한 황토 압밀 플레이트 개발 및 양생방법에 따른 특성 평가

오다영 ( Oh Da-young ),신은미 ( Shin Eun-mi ),최형길 ( Choi Hyeong-gil ),이주나 ( Lee Juna ) 한국복합신소재구조학회 2021 복합신소재구조학회논문집 Vol.12 No.2

목조건축물에 주로 적용되고 있는 철물 접합 시스템은 모재인 나무와 접합부재인 철재 간의 강도 차이 및 재질의 이질성으로 인한 외관상의 위화감 등이 문제점으로 대두되고 있다. 재료 가공의 편의성으로 프리컷 시스템이 도입되었고, 시공성을 해결하기 위한 새로운 재료 및 시스템의 개발이 요구된다. 본 연구에서는 자연재료를 그 원료로 하여 미관상 목조건축물에 위화감이 없는 황토 압밀 플레이트를 개발하였다. 황토 및 소석회를 원료로 하여 제작한 시험체의 양생방법(기건 양생, CO₂ 양생)에 따른 성능을 평가하기 위해 질량 변화, 지압 강도, 흡수율, 표면상태, 열중량변화 측정 및 SEM을 통한 생성광물의 미시적인 부분을 관찰하였다. 또한 제조과정에서의 온실가스 배출 및 흡수에 따른 환경성능평가를 수행하였다. 기건 양생한 시험체에 비해 CO₂ 챔버에 양생한 시험체는 원료인 소석회의 탄산화 반응으로 인한 탄산칼슘의 생성으로 역학적 성능이 향상되었다는 것을 알 수 있었으며, 원료인 소석회의 탄산화반응으로 인해 생석회 제조시 발생하는 CO₂량의 70% 이상을 재흡수 한다는 것을 알 수 있었다. The steel-joint system that is primarily applied to the wooden structure has some problems, i.e., the difference in strength between wood as a base material and steel as a joint, and the inconsistency in appearance owing to the heterogeneity of materials. Herein, a consolidation plate is developed, which is made of natural materials, such as red clay and slaked lime, and goes with the wooden structure in terms of aesthetics. The characteristics of consolidation plates that are manufactured under different curing methods (e.g., air-dry curing, CO₂ curing) are evaluated in terms of mass ratio, bearing strength, thermogravimetric analysis, scanning electron microscope image, and water resistance test. Based on these results, this study proposes the environmental performance evaluation through the analysis of the amount of emission and absorption of CO₂ gas during the manufacturing process. Compared with the air-dry curing specimen, the CO₂ curing specimen showed improved mechanical performance due to the formation of calcium carbonate via carbonation reaction of slaked lime. Furthermore, the CO₂ curing specimen reabsorbed more than 70% of CO₂ gas that was generated during of quick lime production.

울금(Curcuma longa L.) 섭취가 당뇨성 흰쥐의 혈당 및 지질대사 기능 개선에 미치는 영향

오다영 ( Da-young Oh ),강동수 ( Dong-soo Kang ),이영근 ( Young-geun Lee ),김한수 ( Han-soo Kim ) 한국환경과학회 2019 한국환경과학회지 Vol.28 No.5

The purpose of this study was to investigate the improvement effect of 5% turmeric (Curcuma longa L.) on the blood glucose and lipid metabolism function of streptozotocin (STZ, 45 mg/kg body weight)-induced diabetic rats. Seven-week-old male rats were divided into four groups (n=6), and fed experimental diets containing turmeric meal [basal diet+5% turmeric (BT), basal diet+STZ+5% turmeric (ST)], and control (BD), BS groups (basal diet+STZ). The results of this study, turmeric diet groups (BT, ST) in lipid component as evidenced from the significantly reduction of serum total cholesterol, low density lipoprotein-cholesterol (LDL-cholesterol), atherosclerotic index (AI), cardiac risk factor (CRF), triglyceride (TG), phospholipid (PL), free cholesterol, cholesteryl ester, uric acid, blood glucose, non esterified fatty acid (NEFA), and elevation of high density lipoprotein-cholesterol (HDL-cholesterol). The serum albumin and globulin contents were increased in turmeric supplementation diet than STZ-induced diabetic rats (p<0.05). Concentrations of sodium (Na) and chlorine (Cl) in sera were lower in the turmeric diet than diabetic group. Total calcium (T-Ca), phosphorus (Pi) and potassium (K) concentrations in sera were higher in the BT, ST and BD groups than BS group. In vivo experiments with Sprague-Dawley rats showed that ingestion of turmeric (Curcuma longa L.) were effective in the blood glucose and lipid metabolism functional improvement.

울금(Curcuma longa L.)의 용매 별 추출물이 생리활성 및 산화억제에 미치는 영향

오다영(Da-Young Oh),김한수(Han-Soo Kim) 한국유화학회 2018 한국응용과학기술학회지 Vol.35 No.3

울금(Curcuma longa L.)의 사포닌 함량과, 증류수(distilled water, DW), 70% 에탄올 및 노르말 부탄올의 3가지 용매를 사용하여 산화억제 효과를 통한 기능성 식품 소재로서의 가치를 확인한 결과, 총 사포닌 함량은 7.506±0.349 mg SE/g dry weight으로 확인되었다. 추출 수율은 DW (17.11%), 70% 에탄올(15.26%), 노르말 부탄올(4.12%)로 DW에서 가장 높게 나타났으며, 노르말 부탄올 추출물 에서 낮은 수율을 보였다. 항산화 활성은 농도가 높아질수록 활성이 증가되는 것으로 관찰되었다. 총 페놀 함량은 DW, 70% 에탄올, 노르말 부탄올 순으로 각각 8.57, 41.01, 119.11 mg GAE/g의 함량으로 나타났으며, 노르말 부탄올 추출물에서 유의적인 차이를 보이며 높게 관찰되었다(p<0.05). DPPH 라디칼 소거활성은 DW, 70% 에탄올, 노르말 부탄올 순으로 확인되었다. ABTS 라디칼 소거활성, 환원력 및 ferric reducing antioxidant power (FRAP) 또한 DPPH 라디칼 소거활성과 비슷한 양상으로 나타났지 만, ferrous ion 킬레이트 능은 노르말 부탄올(15.87~21.17%), 70% 에탄올(26.10~50.76%), DW (44.47~84.24%) 순으로 측정되었다. 이에, 울금은 산화억제 및 생리활성을 가지는 것으로 규명되었으며 기능성 식품 소재로서의 활용 가능성이 높은 것으로 사료된다. The aim of the present study was to evaluate of turmeric (Curcuma longa L.) on the physiological activities and oxidation inhibitory action. The effects of various solvents (distilled water DW, 70% ethanol and n-butanol) on the total phenolics content (TPC) of turmeric and their corresponding biological activity were studied. Bioactive compound of total saponin 7.506±0.349 mg SE/g dry weight. Turmeric extracts yield were DW (17.11%), 70% ethanol (15.26%) and n-butanol (4.12%), respectively. Oxidation inhibitory action of the samples exhibited a dose-dependent increase. However, in the current study, none of the samples evaluated showed activity as strong as the BHA, ascorbic acid and EDTA. Results showed that extraction solvent had significant effects on TPC and oxidation inhibitory action (DPPH radical scavenging activity, ABTS radical scavenging activity, reducing power and ferric reducing antioxidant power) of n-butanol. Turmeric exhibited the antioxidant properties, which suggests that the plant material could be used for further studies as a potential source for bioactive and natural antioxidant.

목조건축물 접합부재 적용을 위한 황토 압밀 플레이트의 제조 및 성능평가에 관한 연구

오다영(Oh, Da Young),김진성(Kim, Jin Sung),신은미(Shin, Eun Mi),최형길(Choi, Hyeong Gil) 한국콘크리트학회 2019 한국콘크리트학회 학술대회 논문집 Vol.31 No.2