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Fluorescent graphene oxide-peptide-quencher hybrids for detection of cell-secreted protease
양진경,곽선영,전수지,김혜인,강호만,경산,김종호,이윤식 한국공업화학회 2014 한국공업화학회 연구논문 초록집 Vol.2014 No.1
Graphene Oxide (GO) is an emerging luminescent nanomaterial with photo-stability and unique photoluminescence (PL) property. In addition, GO based sensing platform have attracted great attentions due to the featured properties of GO. Herein, we present a GO PL-based optical biosensor consisting of a luminescent GO donor covalently linked with a peptide-quencher complex for the simple, rapid and sensitive detection of proteases. Firstly, we examined the quenching efficiency of candidate quenchers for GO fluorescence, and investigated their quenching mechanism. The designed GO-peptide-quencher system was able to detect the specific proteases, chymotrypsin and matrix metalloproteinase-2 (MMP-2), by turn-on response of quenched GO fluorescence after proteolytic cleavage leading to release of quencher. The GO-peptide-quencher hybrid successfully monitored MMP-2 secreted from living cells with high sensitivity.
Silicoating후 시간지연이 금속과 레진 시멘트 간의 접착강도에 미치는 영향
양진경,배정식,Yang, Jin-Kyoung,Bae, Jeong-Sik 대한치과보철학회 1995 대한치과보철학회지 Vol.33 No.1
For the purpose of this study, the silicoating on Ni-Cr-Be alloy surface was carried out by using the Silicoater MD. The effect of time delay after silicoating on the bond strength between the metal and resin cement was examined. The groups were divided into 4 : group 1 : Sandblasted with $250{\mu}m$ aluminium oxide, immediatly cemented group 2 : Sandblasted with $250{\mu}m$ aluminium oxide, silicoated and immediatly cemented group 3 : Sandblasted with $250{\mu}m$ aluminium oxide, silicoated and cemented after 7 days group 4 : Sandblasted with $250{\mu}m$ aluminium oxide, silicoated and coated with unfilled resin and cemented after 7 days Specimens were luted with Comspan, Superbond C&B or Panavia EX. The tensile bond strength between the metal and resin cement was measured by using the universal testing machine. The results were as follows : 1. The bond strength in groups 2, 3 and 4 was higher than that of group 1 (p<0.05). There was no statistically significant difference among the bond strength in groups 2, 3 and 4 (p>0.05). 2. In Comspan-cemented groups, there was no significant difference among the bond strength in groups 2, 3 and 4 (p<0.05). 3. In Panavia EX-cemented groups, the bond strength of group 3 was higher than that of group 2 (p<0.05) and there was no significant difference between groups 3 and 4. 4. In Superbond C&B-cemented groups, there was significant difference between groups 2 and 3 (p<0.05) and the bond strength of group 4 was higher than that of group 3 (p<0.05). 5. The highest tensile bond strength was obtained by using the Superbond C&B and no difference in the Panavia EX and Comspan. 6. The modes of bond failure were mainly cohesive failure. The method of storage and transport indicated in this study seems recommendable for laboratory and clinical use at least up to 7 days.
Silicoating 후 시간지연이 금속과 레진시멘트 간의 접착강도에 미치는 영향
양진경,배정식 전남대학교 치과대학 1994 전남치대논문집 Vol.6 No.1
For the purpose of this study, the silicoating on Ni-Cr-Be alloy surface was carried out by using the Silicoater MD. The effect of time delay after silicoating on the bond strength between the metal and resin cement was examined. The groups were divided into 4 : group 1 : Sandblasted with 250 ㎛ aluminium oxide, immediately cemented group 2 : Sandblasted with 250 ㎛ aluminium oxide, silicoated and immediately cemented group 3 : Sandblasted with 250 ㎛ aluminium oxide, silicoated and cemented after 7 days group 4 : Sandblasted with 250 ㎛ aluminium oxide, silicoated and coated with unfilled resin and cemented after 7 days. Specimens were luted with Comspan. Superbond C&B or Panavia EX. The tensile bond strength between the metal and resin cement was measured by using the universal testing machine. The results were as follows : 1. The bond strength in groups 2, 3 and 4 was higher than that of group 1 (P<0.05). There was no statistically significant difference among the bond strength in groups 2, 3 and 4 (P>0.05). 2. In Comspan-cemented groups, there was no significant difference among the bond strength in groups 2, 3 and 4 (p>0.05). 3. In Panavia EX-cemented groups, the bond strength of group 3 was higher than that of group 2(p<0.05) and there was no signicant difference between groups 3 and 4. 4. In Superbond C&B-cemented groups, there was significant difference between groups 2 and 3 (p<0.05) and the bond strength of group 4 was higher than that of group 3 (p<0.05). 5. The highest tensile bond strength was obtained by using the Superbond C&B and no difference in the Panavia EX and Comspan. 6. The moles of bond failure were mainly cohesive failure. The method of storage and transport indicated in this study seems recommendable for laboratory and clinical use at least up to 7 days.
저층수 용존산소가 당동만 저서다모류군집 구조의 시간적 변동에 미친 영향
양진경,신현출 한국해양과학기술원 2020 Ocean and Polar Research Vol.42 No.3
This study was carried out at 5 sites 11 times over two years to identify the variation of benthic environments and benthic polychaetous community and analyze the benthic healthiness in Dangdong Bay, a small semi-enclosed inner bay of Jinhae Bay. The temperature of bottom water showed the typical temporal fluctuation of a temperate zone and was in the range of 5.94 ~ 23.94℃. The salinity did not change significantly during the study period and was in the range of 32.93 ~ 35.72 psu. The concentration of dissolved oxygen of bottom water fluctuated a great deal and was in the range of 0.31 ~ 10.20 mg/L. The lowest DO value was recorded in July 2015, as 0.31±0.04 mg/L corresponding to the hypoxic water mass. The hypoxic water mass was formed continuously at some sites also in July and August 2016. The mean grain size was in the range of 7.57 ~ 9.81Ø and the average was 8.89±0.20Ø. The surface sediments were mainly composed of fine sediment (mud) above 85%. The mean of TOC was 3.09±0.22% and LOI was 13.30±0.47%, showing very high levels in Korean coastal waters. The concentration of AVS was in the range of 0.33 ~ 1.28 mgS/g-dry. The high values of organic contents and AVS indicated that there had been the serious organic enrichment in Dangdong Bay. The number of species and the density of the benthic polychaetous community in Dangdong Bay were in the range of 2 ~ 38 species and 2 ~ 2,185 ind./m2 during the study period. The number of species and density were highly sustained in winter and spring, and then decreased gradually with the formation of a hypoxic water mass in summer, and the lowest number of species and density were recorded in autumn. In September and November 2015, the dead zone expanded to almost the whole study area. Dominant polychaetous species were Capitella capitata, Lumbrineris longifolia, Paraprionospio patiens and Sigambra tentaculata, each known as opportunistic species and potential organic pollutant indicator species. In particular, Paraprionospio patiens showed a very high population density of 2,019 ind./m2 in December 2016. Polychaetous communities at each sampling time were classified into 4 temporal groups according to dominant species in each period by cluster analysis and nMDS. ‘Period Group AI’ was formed in winter and spring of 2015, dominated by Capitella capitata, ‘Period AII’in summer dominated by Lumbrineris longifolia, ‘Period B’ in autumn with no fauna in the dead zone, and particularly‘Period C’ in winter of 2016 dominated by Paraprionospio patiens. As a result of analysis of benthic healthiness, the study area was estimated to be in a Fair~Very Poor condition by AMBI and in a Poor~Very Poor condition by BPI during the study period. Both AMBI and BPI showed that the study area was in a Very Poor condition in September and November 2015, and when the dead zone occurred. In Dongdong Bay, the fact that the formation of a hypoxic water mass occurred in summer and a dead zone in autumn were confirmed. In addition, the dominance of opportunistic and organic pollutant indicator species was also observed clearly. The benthic healthiness indexes such as AMBI and BPI showed that organic enrichment was serious in Dangdong Bay.