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시호 모상근 배양에서 생장과 시호 사포닌 생합성에 미치는 배지와 IAA 및 IBA의 영향
안준철,김응식,이현진,황백,Ahn, Jun Cheul,Kim, Eung Sik,Lee, Hyun Jin,Hwang, Baik 한국식물생명공학회 1999 식물생명공학회지 Vol.26 No.3
시호 모상근 (BFHR2 클론) 배양에서 생장과 saikosaponin 생산에 미치는 기본배지, 배지의 다량무기염농도 변화 및 IAA와 IBA의 효과를 조사하였다. 조사한 배양액 중 RCM 배지에 다량무기염농도를 2배로 증가시킨 배지에서 생장이 가장 양호하였다. 또한 2RCM배지에서는 saikosaponin인 a, c와 d의 생성을 확인하였으며, 반면에 MS배지에서는 saikosaponin의 생성이 극히 저조하였다. IBA는 0.5mg/L에서 약 60%의 생장촉진효과를 보였으나, IAA와 IBA두 가지 모두 0.01~5mg/L 까지의 농도에서 뿌리형태의 외적인 변화 없이 saikosaponin 생성을 강하게 억제하는 작용을 나타내었다. We examined the effects of various basal media and exogenous auxins on the growth and saikosaponin production in hairy root cultures of Bupleurum falcatum. Of the various media tested, 2RCM medium was superior for growth and saikosaponin content, in which saikosaponin a, c, and d was produced, however MS medium was resulted in extremely low level in saikosaponin production. By the treatment of 0.5 mg/L IBA the growth rate was increased by about 60% in 2RCM medium, whereas both IAA and IBA showed a potent inhibition effect in saikosaponin biosynthesis by the treatment of 0.01 mg/L~5mg/L.
미치광이풀 ( Scopolia parviflora ) 의 모상근 배양에 의한 Tropane Alkaloid 생산
안준철(Jun Cheul Ahn),정병균(Byung Gyun Jung),백윤웅(Yun Woong Paek),김영준(Young Jun Kim),고경민(Kyeong Min Ko),황성진(Sung Jin Hwang),황백(Baik Hwang) 한국식물학회 1993 Journal of Plant Biology Vol.36 No.3
Transformed hairy roots of Scopolia parviflora, prducing tropane alkaloids and native to Korea, were obtained following infection of rhizome segments with Agrobacterium rhizogenes A_4. Each root tip induced from inoculum sites excised and cultured in MS agar or liquid medium. About seventy of hairy root clones were established. Among them, several fast growing hairy root clones were examined for alkaloid content. Two dimensional TLC analysis showed that the tropane alkaloid pattern of hairy root was more complicated than that in the rhizome of mother plant. On the other hand, some hairy root clones did not produce scopolamine and hyoscyamine. In HPLC analysis, some hairy root clones yield higher levels of scopolamine and hyoscyamine than those of mother plant rhizome which used for infection. Scopolamine and hyoscyamine were identified by comparison of their retention times and of their mass spectra data with those of authentic compounds.
도라지 ( Platycodon grandiflorum ) 뿌리조직에서 고농도 Polyacetylene 함유 모상근의 (毛狀根) 유도 및 배양
안준철 ( Jun Cheul Ahn ),소상섭 ( Sang Seop So ),( Koichiro Shimomura ),황백 ( Baik Hwang ) 한국식물학회 1995 Journal of Plant Biology Vol.38 No.4
Hairy roots of Korean balloon flower (Platycodon grandiflorum A. DC) were induced from the root tissues infected with Agrobacterium rhizogenes ATCC 15834. Growth and polyacetylene [lobetyol (1), lobetyolin (2) and lobetyolinin (3)] production of ten hairy root clones cultured in 1/4 Gamborg B5 (B5) liquid medium were determined. One selected hairy root clone (D6) grew well in hormone free-B5 liquid medium and showed maximum content of polyacetylenes at week 6 for 1 (0.375% dry wt) and at week 7 for 2 and 3 (3.030% and 0.206% dry wt, respectively) whose levels were much higher than those of the intact plant root (1: 0.019%, 2: 0.077% dry wt, 3 was not detected).
시호 ( Bupleurum falcatum L. ) 의 모상근 배양을 통한 Saponin 생산 - 1. 캘러스 , 부정근 , 모상근과 재배근의 Saponin 양상과 함량 비교
안준철(Jun Cheul Ahn),백윤웅(Yun Woong Paek),성충기(Chung Ki Sung),강근형(Geun Hyung Kang),황백(Baik Hwang) 한국식물학회 1993 Journal of Plant Biology Vol.36 No.1
In order to survey possibility to produce saikosaponin from in vitro hairy root culture, culture of callus, adventitous root, and hairy root of Bupleurum falcatum L. were estabilished, and quantitative and qualitative aspects in saikosaponin extracted from thses were compared with those of cultivated root. Callus grew well in MS medium containing 0.9 ㎛ 2,4-D. In contrast, both of adventitous root and hairy root grew well in hormone-free MS medium. However, hairy root showed more rapid growth with extensive lateral root branches, characteristics of lower content of water and softer than in adventitous root. Among the selected lines of adventitous root and hairy root were observed difference in the growth rate. Mannopine, one of opine synthesized in the transformed tissue with Agrobacterium rhizogenes A_4 were detected in the extract of hairy root lines. Pattern and content of crude saponin from adventitous and hairy root showed no difference, but somewhat difference from those of cultivated root. However, in callus, distinct production-aspect of saponin was not observed.
청피홍심무우 ( Raphanus sativus cv. Chungpihongsim ) 의 모상근 배양에 의한 안토시아닌 생성
안준철(Jun Cheul Ahn),백윤웅(Yun Woung Paek),강영희(Young Hee Kang),황백(Baik Hwang) 한국식물학회 1992 Journal of Plant Biology Vol.35 No.1
The hairy root culture of Raphanus sativus cv. Chungpihongsim was established by transformation with Agrobacterium rhizogenes A_4. The transformed roots grew well in adjusted Murashige and Skoog medium to 1/2 basal salts, pH5.2,3% sucrose. Agropine and mannopine, poine synthesized in the transformed tissue were detected in the extract of hairy roots. When 2,4-D and kinetin were added in culture medium of hairy roots, the synthesis of anthocyanin was induced with disorganization of hairy root. Especially, addition of 0.45 μM 2,4-D and 2.3 μM kinetin showed the maximum synthesis of anthocyanin. Pattern of anthocyanin synthesized in transformed roots was somewhat different from that of ordinary roots. However, aglycone part of all anthocyanin was identified as pelargonidin. The content of total anthocyanin in this sample was tentatively calculated 0.49 mg/g fresh weight.
국내양식 민물장어 4 종(Anguilla japonica, A. rostrata, A. bicolor pacifica 및 A. marmorata)의 주요 영양성분의 평가
안준철 ( Jun Cheul Ahn ),정원석 ( Won Seog Chong ),나진호 ( Jin Ho Na ),윤형복 ( Hyoeng Bok Yun ),신경재 ( Kyung Jae Shin ),이경우 ( Kyeong Woo Lee ),박준택 ( Jun Taek Park ) 한국수산과학회 2015 한국수산과학회지 Vol.48 No.1
The basic and main nutritive ingredients of two temperature (Anguilla japonica and A. rostrata) and two tropical ( A. bicolor pacifica and A. marmorata) fresh water eel species that are farmed domestically were evaluated. With exception of A. rostrata, eels cultured at the same farm were used for analysis. The contents of crude protein were in the order A. marmorata (17.7%)> A. rostrata (17.5%)> A. bicolor pacifica (17.4%)> A. japonica (15.8%) and the contents of crude lipids were A. japonica (21.5%)> A. rostrata (15.4%)> A. bicolor pacifica (10.5%)> A. marmorata (8.9%). These values differed significantly even among the three species of eel farmed under identical culture conditions. In comparison, all four species of eel showed similar pattern in overall amino acid composition, although slight differences in the compositions of some amino acids were observed. The fatty acid compositions of muscle tissues were notably different among four species of eel, especially between the tropical and temperature eels. In a taste-test of the meat of the four eel species, which considered taste, flavor and texture, the overall preference was in the order A. japonica, A. marmorata, A. bicolor pacifica and A. rostrata.
홍경천(Rhodiola sachalinensis A. Bor)의 캘러스 배양에서 Salidroside생산
김수정,황백,황성진,안준철,Kim, Soo-Jung,Hwang, Baik,Hwang, Sung-Jin,Ahn, Jun-Cheul 한국식물생명공학회 2004 식물생명공학회지 Vol.31 No.1
본 실험은 기내 배양된 홍경천으로부터 캘러스를 유도하고 가장 잘 자라는 생장조건을 찾기 위해 수행되었다. 실험 결과를 종합해 보면 캘러스의 증식을 위한 배양조건은 최적배지를 조사한 결과,2B$_{5}$배지에서 생장율이 가장 좋았으며, 탄소원으로는 sucrose를 3%로 첨가했을 때 생장율이 가장 높았다. 질소원으로써는 49.6mM KNO$_3$와 2.0mM (NH$_4$)$_2$SO$_4$를 첨가했을 때 생장율이 가장 좋았으며, 인산의 농도는 2.16mM에서 높은 생장율을 보였다. 암배양과 광배양을 비교했을 때 암배양의 경우가 생장율이 높았으며,pH6에서 세포의 생장율이 가장 좋은 조건임을 확인되었다. 기내에서 배양된 캘러스와 자연산 뿌리는 TLC로 분석하여 salidroside를 확인하였으며, HPLC로 salidroside를 정량한 결과, 자연상태 홍경천의 뿌리는 건중량 당 0.17%으로 나타났으며 5% sucrose처리구에서 0.41%의 생산성을 보여, 자연산 홍경천의 뿌리에 비하여 캘러스에서 salidroside의 함량이 높은 것으로 나타났다. Callus of Rhodiola sachalinensis A. Bor were induced from leaf explant on l/2MS solid supplemented with combination of auxin (2.4-D, NAA: 0.1∼2mg/L) and cytokinin (BA: 0.1∼0.2mg/L). The effects of various medium, culture conditions and phytohormones on the growth of callus were investigated. MS, WPM, B$_{5}$ medium and diluted or concentrated media (1/2X, 2X, 3X) were used to investigate the growth of callus on each media. Among these, the highest growth was observed when cultured in in 2B$_{5}$ medium containing 0.5mg/L NAA and 1mg/L BA, 3% sucrose and 49.6 mM KNO$_3$ as nitrogen source, and 2.16mM NaH$_2$PO$_4$ as phoshate source at $25^{\circ}C$ in the dark. The calli cultured with 5% sucrose produced high salidroside content (0.41% on the basis of dry wt) than normal root (0.17%).