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Overexpression of RICE FLOWERING LOCUS T 1 (RFT1) Induces Extremely Early Flowering in Rice
Richa Pasriga,윤진미,조래현,안균흥 한국분자세포생물학회 2019 Molecules and cells Vol.42 No.5
RICE FLOWERING LOCUS T 1 (RFT1) is a major florigen that functions to induce reproductive development in the shoot apical meristem (SAM). To further our study of RFT1, we overexpressed the gene and examined the expression patterns of major regulatory genes during floral transition and inflorescence development. Overexpression induced extremely early flowering in the transgenics, and a majority of those calli directly formed spikelets with a few spikelets, thus bypassing normal vegetative development. FRUITFULL (FUL)-clade genes OsMADS14, OsMADS15, and OsMADS18 were highly induced in the RFT1-expressing meristems. Os-MADS34 was also induced in the meristems. This indicated that RFT1 promotes the expression of major regulatory genes that are important for inflorescence development. RFT1 overexpression also induced SEPALLATA (SEP)-clade genes OsMADS1, OsMADS5, and OsMADS7 in the greening calli before floral transition occurred. This suggested their possible roles at the early reproductive stages. We found it interesting that expression of OsFD1 as well as OsFD2 and OsFD3 was strongly increased in the RFT1-expressing calli and spikelets. At a low frequency, those calli produced plants with a few leaves that generated a panicle with a small number of spikelets. In the transgenic leaves, the FULclade genes and OsMADS34 were induced, but SEP-clade gene expression was not increased. This indicated that Os-MADS14, OsMADS15, OsMADS18, and OsMADS34 act immediately downstream of RFT1.
Rice RHC Encoding a Putative Cellulase is Essential for Normal Root Hair Elongation
문선옥,Anil Kumar Nalini Chandran,김유진,고윤실,홍우종,안균흥,이찬희,정기홍 한국식물학회 2019 Journal of Plant Biology Vol.62 No.1
Root hairs are tubular shaped protuberances ofroot epidermal cells and are found in nearly all vascularplants. Co-ordinate expression of a number of root hairmorphogenesis genes involved in cytoskeleton reorganization,changes in homeostasis and distribution of ion gradients, andcell wall reassembly are required during root hair cellelongation. In this report, we have characterized a root hairspecificputative cellulase gene in rice, OsRHC. OsRHC isspecifically expressed in elongating root hairs and OsRHC istargeted to the plasma membrane. The mutation of the OsRHCgene by a T-DNA knock-out and CRISPR-Cas9 systemcauses a severe reduction in root hair length. Bimolecularfluorescence complementation analysis demonstrated that theOsRHC protein interacts with a root hair-specific cellulosesynthase protein (OsCSLD1) in the plasma membrane. Furthermore, we observed a moderate reduction of cellulosecontent in the osrhc mutant. Our results suggest that theplasma membrane-localized OsRHC plays a critical role incell wall remodeling during root hair extension.