Ursodeoxycholic Acid Inhibits Inflammatory Cytokine Expression in THP-1 Cells Infected with Aggregatibacter actinomycetemcomitans

송유리,김세연,박미희,나희삼,정진 대한구강생물학회 2017 International Journal of Oral Biology Vol.42 No.1

Background: Periodontitis is an inflammatory disease characterized by the breakdown of tooth-supporting tissues, leading to tooth loss. Aggregatibacter actinomycetemcomitans are major etiologic bacterium causing aggressive periodontitis. Ursodeoxycholic acid (UDCA), a hydrophilic gall bladder acid, has been used as an effective drug for various diseases related to immunity. The aim of this study was to investigate the effect of UDCA on the inflammatory response induced by A. actinomycetemcomitans. Methods: A human acute monocytic leukemia cell line (THP-1) was differentiated to macrophage- like cells by treatment with phorbol 12-mystristate 13-acetate (PMA) and used for all experiments. The cytotoxic effect of UDCA was examined by MTT assay. THP-1 cells were pretreated with UDCA for 30 min before A. actinomycetemcomitans infection and the culture supernatant was analyzed for various cytokine production by ELISA. The effect of UDCA on bacterial growth was examined by measuring optical densities using a spectrophotometer. Results: UDCA showed no cytotoxic effect on THP-1 cells, up to 80 μM Ed highlight: Please confirm technical meaning. UDCA pretreatment inhibited the A. actinomycetemcomitansinduced IL-1β, TNF-⍺, and IL-17A secretion in a dosedependent manner. UDCA also inhibited IL-21 production at 60 μM. The production of IL-12 and IL-4 was not influenced by A. actinomycetemcomitans infection. Conclusion: These findings indicate that UDCA inhibits the production of inflammatory cytokines involved in innate and Th17 immune responses in A. actinomycetemcomitansinfected THP-1- derived macrophages, which suggests its possible use for the control of aggressive periodontitis.

치과대학 연극과 졸업생 오종우

송유리 대한치과의사협회 1997 대한치과의사협회지 Vol.35 No.7

병원간호사의 환자안전관리 중요성 인식과 환자안전관리 활동이 간호업무성과에 미치는 영향

송유리,유혜숙 한국웰니스학회 2021 한국웰니스학회지 Vol.16 No.3

본 연구는 종합병원에 근무하는 간호사의 환자안전관리 중요성 인식과 환자안전관리 활동이 간호업무성과에 미치는 요인을 규명하기 위한 서술적 조사연구이다. 연구는 300병상 이상의 종합병원 3개 의료기관 내 병동에 근무하는 간호사 196명의 설문지를 spss25.0 프로그램을 이용하여 빈도분석, 상관관계분석, 다중회기분석을 실시하였다. 그 결과, 간호업무성과는 환자안전관리 중요성 인식(r=.74, p<.001), 환자안전관리 활동(r=.70, p<.001)에서 유의한 양의 상관관계를 보였으며, 환자안전관리 중요성 인식(β=.46, p<.001), 환자안전관리 활동(β=.33, p<.001), 총 임상근무경력 1년 미만(β=-.18, p<.001), 환자안전지침이 있는 경우(β=-.09, p<.05)에서 간호업무성과가 높았으며 이 요인들의 설명력은 64.8%였다. 본 연구결과를 근거로 환자안전관리 중요성 인식과 환자안전관리 활동에 대한 지속적이고 체계적인 교육이 필요할 것으로 사료되며, 이를 향상시키기 위한 병원 차원의 정책적 방안이 필요하다. This study is a descriptive research conducted to identify the factors associated with the perception of importance of patient safety management and the corresponding patient safety management activities that influence the nursing performance of general hospital nurses. The study conducted frequency analysis, correlation analysis, and multiple regression analysis using SPSS 25.0 on questionnaires distributed to 196 nurses working in hospital wards with more than 300 beds in three general hospitals. The results showed that nursing performance had a significant positive correlation with the perception of importance of patient safety management (r=.74, p<.001) and patient safety management activities (r=.70, p<.001). Furthermore, nursing performance was high when nurses perceived the importance of patient safety management (β=.46, p<.001); performed patient safety management activities (β=.33, p < .001); had less than one year of total clinical work experience (β=−.18, p<.001); and were provided with patient safety guidelines (β=−.09, p <.05). The explanatory power of such factors was 64.8%. Based on the results of the study, continual and systematic training is required to improve the perceived importance of patient safety management and patient safety management activities, and hospital-wide policy measures are necessary to achieve such improvements.

SMPS Technology 웹사이트 소개

송유진(宋裕珍) 전력전자학회 2009 전력전자학회지 Vol.14 No.4

수전해용 술폰화 폴리에테르 에테르 케톤과 셀룰로오스 고분자 복합막의 제조 및 특성

송유리,차진산,윤영요,문상봉,정장훈 한국수소및신에너지학회 2016 한국수소 및 신에너지학회논문집 Vol.27 No.5

Polyether ether ketone (PEEK) composite membranes have been intensively investigated for polymerelectrolyte membrane water electrolysis (PEMWE). Covalently linked (CL) sulfonated polyether ether ketone(SPEEK) and cellulose polymer composite membranes were prepared and characterized. Polyether ether ketone(PEEK) and cellulose were sulfonated and then were covalently linked by 1,4-diiodobutane to produce covalentlylinked SPEEK and cellulose polymer composite membranes. The composite membranes showed better thermostabilityand electrochemical properties than SPEEK. The membranes were prepared by sol-gel casting method. CL-SPEEK/Cellulose composite membrane featured 0.2453 S/cm of proton conductivity at 80°C which was betterthan that of Nafion.

Chracterization of THP-1 Cell Death Induced by Porphyromonas gingivalis Infection

송유리,김세연,박미희,나희삼,정진 대한구강생물학회 2017 International Journal of Oral Biology Vol.42 No.1

Background: Periodontitis is generally a chronic disorder characterized by the breakdown of tooth-supporting tissues. P. gingivalis, a Gram-negative anaerobic rod, is one of the major pathogens associated with periodontitis. Frequently, P. gingivalis infection leads to cell death. However, the correlation between P. gingivalis–induced cell death and periodontal inflammation remains to be elucidated. Among cell deaths, the death of immune cells appears to play a significant role in inflammatory response. Thus, the aim of this study was to examine P. gingivalis–induced cell death, focusing on autophagy and apoptosis in THP-1 cells. Methods: Human acute monocytic leukemia cell line (THP-1) was used for all experiments. Autophagy induced by P. gingivalis in THP-1 cells was examined by Cyto ID staining. Intracellular autophagic vacuoles were observed by fluorescence microscopy using staining Acridine orange (AO); and 3-methyladenine (3-MA) was used to inhibit autophagy. Total cell death was measured by LDH assay. Cytokine production was measured by an ELISA method. Results: P. gingivalis induced autophagy in an MOI-dependent manner in THP-1 cells, but 3-MA treatment decreased autophagy and increased the apoptotic blebs. P. gingivalis infection did not increase apoptosis compared to the control cells, whereas inhibition of autophagy by 3-MA significantly increased apoptosis in P. gingivalis-infected THP-1 cells. Inhibition of autophagy by 3-MA also increased total cell deaths and inflammatory cytokine production, including IL-1β and TNF-⍺. Conclusion: P. gingivalis induced autophagy in THP-1 cells, but the inhibition of autophagy by 3-MA stimulated apoptosis, leading to increased cell deaths and pro-inflammatory cytokines production. Hence, the modulation of cell deaths may provide a mechanism to fight against invading microorganisms in host cells and could be a promising way to control inflammation.

Power Electronics Technology 온라인 매거진 웹사이트 소개

송유진(宋裕珍) 전력전자학회 2009 전력전자학회지 Vol.14 No.3

스마트 그리드 기술동향정보 웹사이트 소개

송유진(宋裕珍) 전력전자학회 2010 전력전자학회지 Vol.15 No.4

학회별세션 2 : 서민에 대한 사회과학적 해명 (비판과 대안을 위한 사회복지학회 ; 서민 귀속의식의 결정요인

송유진 한국사회보장학회 2015 한국사회보장학회 정기학술대회 Vol.2015 No.2

Current research on the roles of computer in education

송유재 이화여자대학교 한국문화연구원 1979 韓國文化硏究院 論叢 Vol.33 No.-

정보의 홍수 속에서 현대교육이 당면하고 있는 여러 과제를 해결하기 위한 하나의 수단으로서 컴퓨터의 역할에 대한 기대가 점차 높아가고 있다. 이 논문에서는 최근 연구 보고서를 중심으로 하여 컴퓨터가 교육체제 내에서 행하고 있는 기능과 역할을 CAI, AAAMI, Simulation & Games, 상담, 시간표, 조정, 시험채점 및 분석 등의 관점에서 살펴 보았다. 연구 보고서 분석에 아서 컴퓨터와 개발과정, 특징, 컴퓨터 언어 및 교육에 있어서의 컴퓨터의 포괄적인 기능이 먼저 다루어졌고, 학생과 컴퓨터간의 상호작용, 행정적인 측면에서의 컴퓨터 사용 및 주요 컴퓨터 센터가 소개되었다. 연구 분석결과를 보면 컴퓨터의 교육체계 속에서의 역할이 중요하고 잠재적인 힘을 지니고 있으나 아직도 그 힘을 완전히 발휘하지 목하고 있다는 점과 효과면에 있어서는 연구 결과가 상반되고 있음을 나타내고 있다. 교사역할 변화에 따른 조사, 복합변인을 사용한 고차적인 연구와 선험연구가 필요하며, 또한 질적으로 우수한 연구자의 양성도 시급함이 지적되었다. 앞으로의 컴퓨터의 효과적인 사용은 결국 사용자인 우리에게 달려 있는 것이다.