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도호정 ( Ho-jeong Do ),신예슬 ( Ye-sle Shin ),이재환 ( Jae-hwan Lee ),안용준 ( Yong-jun Ahn ),하인혁 ( In-hyuk Ha ),이윤재 ( Yoon-jae Lee ),김미령 ( Mi-riong Kim ),고원일 ( Won-il Ko ),송설희 ( Seol-hee Song ),정화진 ( Hwa-jin Chu 대한한의학회 한방재활의학과학회 2016 한방재활의학과학회지 Vol.26 No.4
Objectives Danggwisu-san (Dangguixu-san) is a herbal prescription frequently used to treat pain or swelling caused by contusion. To determine the expiration period through scientific methodology, stability of Danggwisu-san (Dangguixu-san) water extract, a herbal medicine, was examined under various storage conditions and periods. Methods Danggwisu-san (Dangguixu-san) was stored either at room temperature (23±2℃), under a refrigerating condition (4℃) or under a freezing condition (-18±2℃) for 0, 1, 2, 3 or 4 weeks and then freeze-dried. Total phenol and total flavonoid amounts were investigated; contents of amygdalin (Prunus persica), paeoniflorin (Paeonia lactiflora), and glycyrrhizin (Glycyrrhiza uralensis) - the marker compounds of Danggwisu-san (Dangguixu-san) - were also analyzed through high performance liquid chromatography (HPLC). Results No significant change in total phenol and total flavonoid amounts was observed under the indicated storage conditions. Moreover, the contents of marker compounds, i.e. amygdalin, paeoniflorin, and glycyrrhizin, did not alter significantly under the indicated conditions, as well. Conclusions Danggwisu-san (Dangguixu-san) was found to be stable up until 4 weeks under the indicated conditions. Further studies on efficacy and long-term stability are warranted to establish the expiration period of Danggwisu-san (Dangguixu-san). (J Korean Med Rehabil 2016;26(4):1-8)