부산직할시 : 끈질긴 유대로부터의 도시사회건설을

손혜련 대한지방행정공제회 1967 地方行政 Vol.16 No.164

Total Polyphenol, Flavonoid Contents and Antioxidant Activities of Dolsan Leaf Mustard Pickle during Storage

손혜련,오선경,최명락 한국생물공학회 2016 KSBB Journal Vol.31 No.2

Dolsan leaf mustard has various biological functions, including those in the immune system and cancer prevention. It contains high amounts of nutritional and medicinal compounds, which are important for maintaining optimum health. The objective of this study was to determine the total phenolics contents and antioxidant activity in Dolsan leaf mustard pickle (DLMP) during storage. DLMP methanol extracts had the highest total polyphenol and flavonoid contents of 35.56±0.01 GAE mg/g and 4.54±0.03 QE mg/g, respectively. The DPPH and ABTS radical scavenging activities in the DLMP methanol extracts showed the highest activities of 79.4 and 85.36%, respectively. The ferric reducing antioxidant power (FRAP) assay showed 2.24-3.82 mM FeSO4 eq. (p<0.05) in DLMP extracts. Overall, storage at day 14 showed the highest antioxidant activity.

Quality Characteristics of Dolsan Leaf Mustard according to Various Blanching Conditions and Liquid Chromatography with Photodiode Array and Tandem Mass Spectrometry Analysis

손혜련,오선경,최명락,Son, Hae-Reon,Oh, Sun-Kyung,Tsukamoto, Chigen,Choi, Myeong-Rak The Korean Society for Biotechnology and Bioengine 2016 KSBB Journal Vol.10 No.5

This study aims to examine the optimum blanching conditions as a pretreatment condition to improve the storage stability of Dolsan leaf mustard pickle. The effects of the blan- ching temperature and time were investigated at a temperature range of $80-100^{\circ}C$. Sampling was done for 1 month after a 5 days interval. The L value of the Dolsan leaf mustard was found to be the highest at $80^{\circ}C$. The cutting force increased as the blanching temperature increased. The tensile strength decreased at $95^{\circ}C$ and $100^{\circ}C$. In addition, the sensory evaluation scores were the best at $80^{\circ}C$. The storage stability was assessed at various blanching temperatures to increase the sinigrin content during storage. Liquid chromatography with photodiode array and tandem mass spectrometry (LC-PDA/MS/MS) analysis was conducted to identify and quantify the sinigrin content in the Dolsan leaf mustard. Sinigrin as an internal standard was co-injected into each sample solution. The sample was monitored by recording the ultraviolet absorbance at 228 nm and by electrospray ionization (ESI) positive ion mode in the m/z 50-1,500 range. Blanching the sample at $80^{\circ}C$ showed the highest sinigrin concentration during storage among various temperatures and the maximum concentration was 350 ppm at 15 days storage. Study on utilization of vegetable from food processing of leaf mustard and preservation conservation results suggest that blanching at $80^{\circ}C$ is expected to improve the palatability of the pickle.

Total Polyphenol, Flavonoid Contents and Antioxidant Activities of Dolsan Leaf Mustard Pickle during Storage

손혜련,오선경,최명락,Son, Hae Reon,Oh, Sun Kyung,Choi, Myeong Rak The Korean Society for Biotechnology and Bioengine 2016 KSBB Journal Vol.6 No.2

Dolsan leaf mustard has various biological functions, including those in the immune system and cancer prevention. It contains high amounts of nutritional and medicinal compounds, which are important for maintaining optimum health. The objective of this study was to determine the total phenolics contents and antioxidant activity in Dolsan leaf mustard pickle (DLMP) during storage. DLMP methanol extracts had the highest total polyphenol and flavonoid contents of $35.56{\pm}0.01GAEmg/g$ and $4.54{\pm}0.03QEmg/g$, respectively. The DPPH and ABTS radical scavenging activities in the DLMP methanol extracts showed the highest activities of 79.4 and 85.36%, respectively. The ferric reducing antioxidant power (FRAP) assay showed 2.24-3.82 mM $FeSO_4$ eq. (p<0.05) in DLMP extracts. Overall, storage at day 14 showed the highest antioxidant activity.

ACE and α-glucosidase Inhibitory Activity of the Glucosinolates in Dolsan Leaf Mustard Pickle during Storage

손혜련,오선경,최명락,Son, Hae-Reon,Oh, Sun-Kyung,Tsukamoto, Chigen,Choi, Myeong-Rak The Korean Society for Biotechnology and Bioengine 2016 KSBB Journal Vol.17 No.3

This study was carried out for investigate that physiological activity, quantification and qualitative were sinigrin of Dolsan leaf mustard pickle (DLMP) during storage. sinigrin contains high amounts of nutritional and medicinal compounds, which are important for maintaining optimum health. ACE inhibitory activity was ranged between 43.2 and 79.4%. DLMP methanol extracts demonstrated highest ACE inhibitory activity at 79.4% on day 14, whereas DLMP ethanol extracts demonstrated highest Angiotensin I-converting Enzyme (ACE) inhibitory activity of 43.2% at day 0. The ${\alpha}-glucosidase$ inhibitory activity of positive control 0.02% (v/v) acarbose was 78%. The DLMP methanol extracts had the highest ${\alpha}-glucosidase$ inhibitory activity at 64.0% on day 14, whereas DLMP ethanol extracts had the lowest ${\alpha}-glucosidase$ inhibitory activity of 42.8% at day 28. Sinigrin was high in DLMP methanol extracts at $49.55{\mu}g/ml$ on day 14 of storage. Sinigrin standard was eluted at 2.73 min and MS analysis was m/z 283.03 along with fragment ions at m/z 204 and 149.06. These data show that sinigrin formed desulfo-glucosinolates $[M-SO_3-2H_2O+K+2H]^+$. Sinigrin concentration increased until day 14 and then decreased after that. DLMP methanol extracts had consistently higher sinigrin concentration than DLMP acetonitrile extracts during 28 days of storage.

저장기간 동안 돌산 갓 피클의 이화학적 및 관능적 특성 분석

손혜련(Hae-Reon Son),오선경(Sun-Kyung Oh),배상옥(Sang-Ok Bae),최명락(Myeong-Rak Choi) 한국생명과학회 2015 생명과학회지 Vol.25 No.8

본 연구에서는 돌산 갓을 이용하여 저염 피클을 개발하기 위한 기초적인 연구로 저장기간 중 이화학적 및 관능변화에 대해 분석하였다. 돌산 갓 피클을 -2℃에서 90일 동안 저장하였다. 염도는 1.63~1.85% 범위를 유지하였다. pH는 저장 0일째 약 4.5에서 저장 90일째 4.37로 감소하였다. 산도는 저장기간 중 60일째까지 점진적으로 증가하다가 이후 약 1.10%로 급격히 증가하였다. 색도의 L값은 잎에서 30일째까지 증가하였다가 이후 감소하였고, 줄기에서는 저장기간 동안 감소하였다. 저장기간 동안 a값은 증가하였고, 반면에, b값은 감소하여 갈변 현상이 진행된 것을 알 수 있었다. 유산균 수는 0일째 DMP잎에서 1.4×10³ CFU/ml, DMP 줄기에서 5.6×10³ CFU/ml로 나타났고 이후 점차 감소하였다. 관능검사 결과는 아삭거림이 30일째 감소하였고, 질긴 정도는 증가하였다. 신맛은 증가하였고, 짠맛은 감소하였다. 90일간 저장기간 동안에도 연부현상이 발생하지 않았다. 그래서 최적의 돌산 갓 피클은 30일째 적합하다고 판단되며 돌산 갓은 저염 피클제품으로서의 이용 가능성을 제시하고, 수입 위주의 피클을 대체함과 동시에 돌간 갓을 이용한 식품의 다양화에 기여하고자 분석을 실시하였다. The principal objective of this fundamental research was to analyze the physicochemical changes and sensory properties of Dolsan leaf mustard pickles (DMP) for the development of low-salt pickles in order to contribute to the diversification of food. The DMPs were stored at -2°C for 90 days, with salinity maintained at 1.63% to 1.85%. The pH was decreased during storage, and the acidity was gradually increased for up to 60 days of storage, after which it was increased rapidly to 1.10. The L-values (lightness) from the stems were decreased during storage. The a-values (greenness) were increased, while the b-values (yellowness) were decreased. It was shown that the DMP had a progressive browning phenomenon and the number of lactic acid bacteria gradually decreased. The sensory evaluation showed that crunchy textures were decreased and touch textures were increased at 30 days. Therefore, Dolsan leaf mustard is suitable as a material for low-salt pickles during storage for 90 days. The results of the analysis of the physicochemical and sensory properties indicate that the optimal storage for DMP was maintained for up to 30 days.

합리,정서,행동치료를 바탕으로 한 독서치료가 아동의 일상적 스트레스와 스트레스 대처행동에 미치는 효과

손혜련 ( Hye Ryeon Son ),김춘경 ( Choon Kyung Kim ) 한국정서.행동장애아교육학회(구.한국정서학습장애아교육학회) 2012 정서ㆍ행동장애연구 Vol.28 No.1

본 연구는 아동의 일상적 스트레스 감소와 긍정적인 스트레스 대처행동의 증가를 위하여 합리·정서·행동치료를 바탕으로 한 독서치료 프로그램을 개발하고, 이 프로그램이 아동의 일상적 스트레스와 스트레스 대처행동에 미치는 효과를 검증하는 데 목적이 있다. 연구 대상은 대구광역시 ○○초등학교 4학년 216명 아동에게 아동의 일상적 스트레스 검사와 스트레스 대처행동 검사를 실시하여 스트레스 수치가 높은 고위험군을 선정하여 그 중 다시 각 12명씩 실험집단과 통제집단으로 나누었다. 실험집단에는 본 연구자가 개발한 합리·정서·행동치료를 바탕으로 한 독서치료 프로그램을 주 2회, 50분, 12회기에 걸쳐 실시하였고, 통제집단은 아무런 처치도 하지 않았다. 프로그램의 효과를 분석하기 위해서 아동의 일상적 스트레스 척도, 스트레스 대처행동 척도를 사용하여 각각 사전, 사후 검사를 실시하였고, 4주 후에 추후 검사를 실시하였다. 연구결과 합리·정서·행동치료를 바탕으로 한 독서치료 프로그램은 아동의 일상적 스트레스를 감소시키고 긍정적인 스트레스 대처행동을 증가시키는 데 유의미한 효과가 있는 것으로 나타났고, 그 효과는 추후에도 계속 유지되었다. The purpose of this study is to develop the ``Literature Therapy Programs based on Rational Emotive Behavior Therapy`` for children and verify the effects of this program regarding daily stress in children and their strategies for coping with stress. Participants consisted of 40 high risk children who showed high levels of stress in the pretest, which includes daily stress test and coping strategies test, who were chosen from among 216, 4th grade Daegu ○○ elementary school children. Among them, 24 children whose parents agreed with this program finally joined, and they were divided by the treatment group and control group at random. (their genders were divided evenly to both groups) The treatment group took part in the literature therapy program twice a week (totally 12 times for 7 weeks, 1 session lasts 50 minutes) - from September 9 to October 21 in 2009. This study conducted pre and post tests and follow-up tests between two groups using the Scales of Daily Stress in Children and the Scales of Strategies for coping with Children`s Stress to analyse the effects of this program. Consequently, Literature therapy Programs based on Rational Emotive Behavior Therapy reduce daily stress in children and have an effect on increasing children`s positive strategies for coping their stress.

Total Polyphenol, Flavonoid Contents and Antioxidant Activities of Dolsan Leaf Mustard Pickle during Storage

Hae Reon Son(손혜련),Sun Kyung Oh(오선경),Myeong Rak Choi(최명락) 한국생물공학회 2016 KSBB Journal Vol.31 No.2

Dolsan leaf mustard has various biological functions, including those in the immune system and cancer prevention. It contains high amounts of nutritional and medicinal compounds, which are important for maintaining optimum health. The objective of this study was to determine the total phenolics contents and antioxidant activity in Dolsan leaf mustard pickle (DLMP) during storage. DLMP methanol extracts had the highest total polyphenol and flavonoid contents of 35.56±0.01 GAE mg/g and 4.54±0.03 QE mg/g, respectively. The DPPH and ABTS radical scavenging activities in the DLMP methanol extracts showed the highest activities of 79.4 and 85.36%, respectively. The ferric reducing antioxidant power (FRAP) assay showed 2.24-3.82 mM FeSO4 eq. (p<0.05) in DLMP extracts. Overall, storage at day 14 showed the highest antioxidant activity.

ACE and α-glucosidase Inhibitory Activity of the Glucosinolates in Dolsan Leaf Mustard Pickle during Storage

Hae-Reon Son(손혜련),Sun-Kyung Oh(오선경),Chigen Tsukamoto,Myeong-Rak Choi(최명락) 한국생물공학회 2016 KSBB Journal Vol.31 No.3

This study was carried out for investigate that physiological activity, quantification and qualitative were sinigrin of Dolsan leaf mustard pickle (DLMP) during storage. sinigrin contains high amounts of nutritional and medicinal compounds, which are important for maintaining optimum health. ACE inhibitory activity was ranged between 43.2 and 79.4%. DLMP methanol extracts demonstrated highest ACE inhibitory activity at 79.4% on day 14, whereas DLMP ethanol extracts demonstrated highest Angiotensin I-converting Enzyme (ACE) inhibitory activity of 43.2% at day 0. The α-glucosidase inhibitory activity of positive control 0.02% (v/v) acarbose was 78%. The DLMP methanol extracts had the highest α-glucosidase inhibitory activity at 64.0% on day 14, whereas DLMP ethanol extracts had the lowest α-glucosidase inhibitory activity of 42.8% at day 28. Sinigrin was high in DLMP methanol extracts at 49.55 μg/ml on day 14 of storage. Sinigrin standard was eluted at 2.73 min and MS analysis was m/z 283.03 along with fragment ions at m/z 204 and 149.06. These data show that sinigrin formed desulfo-glucosinolates [M-SO3-2H2O+K+2H]+. Sinigrin concentration increased until day 14 and then decreased after that. DLMP methanol extracts had consistently higher sinigrin concentration than DLMP acetonitrile extracts during 28 days of storage.

Quality Characteristics of Dolsan Leaf Mustard according to Various Blanching Conditions and Liquid Chromatography with Photodiode Array and Tandem Mass Spectrometry Analysis

Hae-Reon Son(손혜련),Sun-Kyung Oh(오선경),Chigen Tsukamoto,Myeong-Rak Choi(최명락) 한국생물공학회 2016 KSBB Journal Vol.31 No.1

This study aims to examine the optimum blanching conditions as a pretreatment condition to improve the storage stability of Dolsan leaf mustard pickle. The effects of the blan- ching temperature and time were investigated at a temperature range of 80-100℃. Sampling was done for 1 month after a 5 days interval. The L value of the Dolsan leaf mustard was found to be the highest at 80℃. The cutting force increased as the blanching temperature increased. The tensile strength decreased at 95℃ and 100℃. In addition, the sensory evaluation scores were the best at 80℃. The storage stability was assessed at various blanching temperatures to increase the sinigrin content during storage. Liquid chromatography with photodiode array and tandem mass spectrometry (LC-PDA/MS/MS) analysis was conducted to identify and quantify the sinigrin content in the Dolsan leaf mustard. Sinigrin as an internal standard was co-injected into each sample solution. The sample was monitored by recording the ultraviolet absorbance at 228 nm and by electrospray ionization (ESI) positive ion mode in the m/z 50-1,500 range. Blanching the sample at 80℃ showed the highest sinigrin concentration during storage among various temperatures and the maximum concentration was 350 ppm at 15 days storage. Study on utilization of vegetable from food processing of leaf mustard and preservation conservation results suggest that blanching at 80℃ is expected to improve the palatability of the pickle.