연구논문 : 자연과학 ; 남부지역에서 비닐피복에 따른 콩 생육특성 및 수량성 차이

백인열 ( In Youl Baek ) 한국국제농업개발학회 2012 韓國國際農業開發學會誌 Vol.24 No.1

Nowadays P. E. film mulching for weed control and moisture preservation is popular in soybean cultivation of southern region of Korea. Some merits of P. E. film mulching are moisture preservation, soil aggregation, weed control and growth promotion due to increase soil temperature. The rearrangement of seeding date of soybean is necessary to prevent overgrowth of soybean and promote healthy growth. As global warming increases average world temperature, the demanding of re-establishing soybean cultivation system, such as seeding date, has been increased. Therefore, we discussed seeding date accompanying maturity types as soybean was cultivated on condition of P. E. film mulching. Four varieties, Saeol, Seonyu, Daewon and Pungsannamul were seeded on May 10, May 25, June 10 and June 25 in P. E. film mulching and non-mulching field. It is required more mean days of flowering in nonmulching and more days of maturity in P. E. film mulching. In R5 stage completed organ structure dry matter weight was heavier and LAI was ranged 5 to 7 which was optimum LAI. Number of pods and number of seeds per plant in P. E. film mulching were higher on June 25 seeding and 100-seed weight was heavier through all seeding date of P. E. film mulching. Seed yield in P. E. film mulching cultivation was higher on May 25 and June 10 seeding. Occurrence of damaged seeds showed no difference between two cultivation types and among seeding dates. Major diseases and insect pests were bacterial pustule, black root rot, wild fire and stink bugs. And that, occurrence of root rot and stink bugs influenced to yield decrease. Protein content of soybean seed in P. E. film mulching was lower in all seeding dates. Fatty acid compositions showed no difference between two culivation types and among seeding dates. But C18:1 composition was higher on May 10 and May 25 seeding in P. E. film mulching cutivation and C18:3 composition was similar between two cultivation types. Isoflavone content was higher in P. E. film mulching cultivation and higher as seeding date was delayed. Summerizing above results optimum seeding date for P. E. film mulching cultivation ranged from May 25 to June 10 in southern region of Korea.

연구보문 : 콩 비닐피복 재배시 최적적심방법 연구

신상욱 ( Sang Ouk Shin ),김현태 ( Hyun Tae Kim ),이영훈 ( Young Hoon Lee ),정찬식 ( Chan Sik Jeong ),이병원 ( Byong Won Lee ),고병구 ( Byong Gu Ko ),백인열 ( In Youl Baek ),박금룡 ( Keum Yong Park ) 한국국제농업개발학회 2010 韓國國際農業開發學會誌 Vol.22 No.4

콩의 비닐피복 재배시에 최적적심 방법을 구명하고자 본 시험에서 적심방법별 주요 특성 및 수량 비교한 결과는 다음과 같다. 1. R5 stage의 건물중은 두 품종 모두에서 V8 stage의 순적심과 개화시 적심에서 많았는데 이들 처리는 대원콩에서는 무처리 보다 가벼웠고 신부석태에서는 무처리 보다 무거웠다. 그리고 이들 처리는 최적엽면적 지수을 확보하였다. 2. 포장도복은 V8 stage의 순적심에서는 발생하지 않았고 개화시 적심에서는 2~5 정도의 도복이 발생하였다. 3. 개화기는 품종별 처리간 모두 같았고, 성숙기는 대원콩에서 1~2일의 차이를 보였지만 신부석태는 차이가 없었다 4. 종실비대기간인 R5 stage에서 R7 stage까지의 두 품종 평균 순동화량(NAR)은 V8 stage의 순적심과 개화시 적심에서 비슷하면서 가장 높았다. 5. 두 품종 평균수량은 V8 stage의 순적심과 개화시 적심에서 가장 높았다. 6. 외관적 품질에서는 V8 stage의 순적심에서 백립중이 가장 무거웠고 미숙립 및 다른 피해립율도 낮았다. This experiment was carried out to make clear optimum decapitation method for yield increase of soybean and to reduce lodging as soybean is cultivated in vinyl mulching. Results are as follow; In R5 stage, dry matter weight in two varieties was higher in V8 (terminal) treatment and flowering initiation stage treatment which were lighter than control in Daewon and heavier in Sinbuseoktae. Also, LAI(Leaf Area Index) in these two treatments was suitable. Lodging index in V8 (terminal) treatment was not occurred whereas in flowering initiation stage treatment was ranged from 2 to 5. Flowering date in two varieties was not differant among five treatments and maturity date was similar. NAR (Net Assimilation Rate) from R5 to R7 stage was higher in both V8 (terminal) and flowering initiation stage treatment showing similar mean value. Although mean values was not significantly different, the yield of terminal decapitation in V8 stage which was similar to flowering initiation stage treatment was higher. Also, 100-seed weight of terminal decapitation in V8 stage was the heaviest and apparent seed quality was favorable. Consequently, the optimum decapitation method in vinyl mulching cultivation of soybean was terminal decapitation in V8 stage.

풋콩 수확 전후의 당 및 토코페롤 변화 양상

고종민 ( Jong Min Ko ),하태정 ( Tae Joung Ha ),김현태 ( Hyun Tae Kim ),한원영 ( Won Young Han ),이병원 ( Byeong Won Lee ),전명기 ( Myeong Gi Jeon ),윤홍태 ( Hong Tae Yun ),백인열 ( In Youl Baek ) 한국콩연구회 2011 韓國콩硏究會誌 Vol.28 No.1

Soybeans undergo many compositional changes during the seed filling stage, and green vegetable soybeans also occur the changes before and after harvest. First experiment was carried out to investigate the changing patterns of sugars of vegetable soybean by storage temperature and period after harvest. When green pods of vegetable soybeans were stored in 25˚C after harvest, the sucrose contents were drastically decreased above 60 percent from 8.7~10.4% to 3.0~3.1% only a day after harvest. On the other hand, when vegetable soybeans were stored in -20˚C immediately after harvest, the sucrose contents have maintained 86.5~90.8% in eleven days after harvest compared with the content of harvesting day, and in 4˚C have kept 75.9~79.8%. These results show that green pods of vegetable soybean must keep in low temperature on the day of harvest to improve a sweet taste. In second experiment, the changing patterns of tocopherol in immature soybean were analyzed using 3 cultivars, Hwaeumput, Danmi and Danmi2. Danmi2 having the highest content was 435.5 μg/g in 35 days after flowering. The contents of total tocopherol in 45 days were decreased to 43.6% in Hwaeumput, 42.4% in Danmi and 68.7% in Danmi2 respectively, compared with the content of 35 days. Even if the decreasing patterns were similar among 3 cultivars, Danmi2 showed the highest content and the slowest reduction. These results imply that it is important the choice of variety for production of vegetable soybean possessing high tocopherol.

우리나라 콩 육종의 현황과 연구발전 계획

백인열 ( In Youl Baek ),박금룡 ( Keum Yong Park ) 한국콩연구회 2006 韓國콩硏究會誌 Vol.23 No.1

大豆屬(Glycine spp.)의 形態的 特性에 의한 類緣關係 分析

In Youl Baek(白寅烈),Doo Chull Shin(申斗澈),Hyun Tae Kim(金賢泰),Hyung Soo Suh(徐亨洙),Yong Ho Kwack(郭龍鎬),Yun Jin Oh(吳潤鎭) 한국육종학회 1997 한국육종학회지 Vol.29 No.2

Discovery and transfer of useful characters of wild species into the elite lines may facilitate the development of crop varieties with broad genetic bases. In this study, morphological relationships among fourteen Glycine species was analyzed on basis of twenty-five morphological characters. The upper five principle components contracted from twenty-five morphological characters by the principle component analysis accounted for about 72% of the total variation. The subgenus Soja; G. max, G. gracilis, and G. soja were clearly seperated from the subgenus Glycine; G. arenaria, G. falcata, G. canescens, G. latifolia, G. microphylla, and G. tomentella by the scatter diagrams distributed on the plane of the first and second or the first and third principle components. The cluster analysis and morphological characteristics of G. cyrtoloba and G. curvata were very similar, but they are distinguished from tri-compound leaves at the unifoliate leaf node. Fourteen Glycine species could be classified into eight major groups at the average distance of 0.75 and fourteen sub-groups at the average distance of 0.45, that is, into Group Ⅰ: G. max; Group Ⅱ: A: G. gracilis, B: G. soja; Group Ⅲ: G. microphylla; Group Ⅳ: G. tomentella (2n=38, 40, 78, 80) ; Group Ⅴ: A: G. tabacina (2n=120), B: G. tabacina(2n =40, 80) ; Group Ⅵ: A: G. clandestine, B: G. canescens; Group Ⅶ: A: G. latifolia, B: G. tabacina (2n=40), G. latrobeana, C: G. cyrtoloba, G. curvata; and Group Ⅷ: A: G. falcata, B: G. arenaria.

RAPD方法에 의한 콩屬의 種間 類緣關係 分析

In Youl Baek(白寅烈),Yong Hwi Yoon(尹用輝),Dao Chull Shin(申斗澈),Gyu Hwan Park(朴圭煥),Young Hun Hwang(黃永鉉),Dal Ung Kim(金達雄) 한국육종학회 1997 한국육종학회지 Vol.29 No.3

Effective conservation and the use of plant genetic resources are essential for future agricultural progress. In this study, the genetic relationships among fourteen species of Glycine were analyzed based on the RAPDs. A total of fourteen species (twenty one accessions) in the genus Glycine were identified by the frequency of RAPDs polymorphisms. Ten primers out of thirty primers tested, were selected for the reliability and repeatability of banding patterns. The amplification of the genomic DNA produced eighty two polymorphic band patterns with the average of 8.2 bands per primer. By Nei and Li’s formula, the mean 1-F value (genetic similarity) was 0.461, and the highest value was 0.774 (G. max : G. microphylla) and the smallest one was 0.128 (intraspecies G. max). That of interspecies in the subgenus Glycine was 0.620 and 0.250 in the highest and the lowest cases, respectively. Thus the genetic similarities were much variable within the interspecies of subgenus Glycine but those were somewhat small between the subgenus Glycine and Soja. By UPGMA (unweighted pair group method using an arithmetic average) cluster analysis based on 1-F value, the fourteen species of Glycine could be classified into four major-groups (genetic similarity (GS) : 0.45) and nine sub-groups (GS : 0.35), that is, into Group Ⅰ : subgenus Soja : G. max, G. sofa, G. gracilis; Group Ⅱ : G. falcata ; Group Ⅲ : A : G. latrobeana, G. canescens, G. microphylla, B : G. curvata, G. cyrtoloba, C : G. clandestina, D : G. tabacina(2n=80) ; Group Ⅳ : A : G. arenaria, B : G. tomentella (2n=38, 40, 78, 80), C : G. tabacina (2n=40, 120), G. latifolia. By multidimensional scaling (MDS), the subgenus Glycine and Soja were clearly classified in the clustering of genetic relationship distance. The genetic relationship distance was closely identified between G. max of the cultigen and G. clandestina, G. tomentella, G. cyrtoloba, G. curvata of the subgenus Glycine.

장류용 내병 내도복 다수성 콩 신품종 ‘대하 1호’

백인열(In-Youl Baek),김현태(Hyun-Tae Kim),고종민(Jong-Min Ko),한원영(Won-Young Han),박금룡(Keum-Yong Park),오기원(Ki-Won Oh),하태정(Tae-Joung Ha_,신상욱(Sang-Ouk Shin),윤홍태(Hong-Tae Yun),문중경(Jung-Kyung Moon),오영진(Young-Jin Oh) 한국육종학회 2013 한국육종학회지 Vol.45 No.1

A new soybean cultivar for soy-paste, ‘Daeha 1’, was developed by soybean breeding team in the Yeongnam Agricultural Research Institute (YARI) in 2008. A promising line, SS97214-S-S-S-15, was selected from the combination between ‘Suwon192’ and a pedigree came from cross combination between ‘Jangyeobkong’ and ‘Hwaeomputkong’. It was designated as the name of ‘Milyang 164’. It had good result from regional adaptation yield trial (RYT) for three years from 2006 to 2008 and released as the name of ‘Daeha 1’. It has a determinate growth habit, white flower, grey pubescence, yellow seed coat, yellow hilum, large spherical seed (25.4 grams per 100 seeds). ‘Daeha 1’ is tolerant to soybean mosaic virus and bacterial pustule, the major soybean disease in Korea. The average yield of ‘Daeha 1’ was 2.62 ton per hectare in the regional yield trial (RYT) carried out for three years from 2006 to 2008, which was 5 percent higher than that of check cultivar, ‘Taekwangkong’.

수확기 강우가 콩의 종실 품질에 미치는 영향

강성택 ( Sung Taeg Kang ),이주석 ( Ju Suk Lee ),백인열 ( In Youl Baek ),한원영 ( Won Young Han ),신두철 ( Doo Chull Shin ),정명근 ( Myoung Gun Choung ) 한국콩연구회 2014 韓國콩硏究會誌 Vol.31 No.1

수확기 전후 강우는 종실수량감수 뿐 아니라 종자의 품질저하에 큰 영향을 미치며 차 년도에 종자로서 이용 하는데 큰 문제를 발생시킨다. 기상이변 등에 대응하여 안정적인 콩 생산을 위해서는 이러한 불량환경에 대한 내재해성을 갖춘 품종육성이 필요하다. 본 실험은 수확기 장기 강우에 의한 콩 종실의 품질저하 원인을 파악하고 강우에 의한 피해를 검정할 수 있는 적절한 검정방법을 탐색하여 내재해 품종육성의 기초자료로 활용하고자 실시되었다. 강우에 의한 종실의 품질변화를 살펴보기 위하여 포장상태에서 분수호수를 이용하여 인공강우 처리를 실시하고, 등숙기 강우피해에 대한 간이검정 방법을 탐색코자 비가림 하우스조건에서 인공강우를 실시하여 종자의 변화를 관찰하였다. 1. 포장 인공강우 처리에 의한 종실의 품질변화 정도는 발아율은 성숙이 진전될수록, 그리고 강우처리일수가 길어질수록 발아율 감소하는 경향이었다. 발아율과 발아세의 감소와 부패묘의 증가정도로 살펴본 성숙기 강우에 의한 종실의 피해 정도는 품종간 다소 차이가 있었고, 성숙의 진전에 따라서도 차이가 있었으며 또한 처리기간에 따라서 6일 이상의 처리시 급격한 품질 감소를 가져왔다. 2. 비가림 하우스를 이용한 성숙기 이후 인공강우 처리에 의한 종실의 외관 품질의 변화정도는 비정상립의 비율, 병해립의 비율이 증가하였고, 검정콩 1호, 다원콩, 대원콩, 새알콩, 은하콩, 일품검정콩은 6일간 처리에서부터 비정상립의 비율이 유의하게 높아졌으나 만리콩, 소백나물콩, 풍산나물콩은 12일 처리에서만 유의한 차이를 보여 종실외관 품질에서 품종간 차이를 나타내었다. 3. 인공강우 처리에 의한 종실의 발아관련 형질의 변화는 발아율, 발아세, 유묘부패율, 부패립 및 경실종자비율에서 품종간, 처리간 그리고 품종과 처리간 상호작용에서 고도의 유의성이 인정되고, 발아율은 대부분 6일 이상처리에서 발아율의 감소가 유의하게 나타났으나 발아세는 12일부터는 유의하게 감소하였으나 품종간에 차이를 나타내었다. 4. 인공강우처리에 의한 각 형질별 상관관계에서 발아율과 발아세는 부패묘 및 썩은 종자의 비율과 부의 상관을 가졌고 비건전종자 비율과 발아율은 정의상관을 가져 외관 종실의 형태관찰에 의해서도 어느 정도 종자 활력정도를 추정할 수 있으나 이들의 상관정도는 높지 않았다. Soybean (Glycine max (L.) Merr.) seed may lose its vigor depending on the field environment, which was prior to harvest, during development and maturation on the plant. This study was conducted to evaluate the field weathering effect on seed quality after physiological maturity and to achieve proper screening method with rain-shelter-greenhouse. To evaluate the rainfall effect on seed quality, the plant was subject to an artificial rainfall treatment in the field and greenhouse that was able to control rainfall and sunshine. After artificial rainfall treatment, seed vigor was decreased according to seed maturation degree and rainfall treatment duration. To screen tolerant soybean varieties, sufficient filed weathering has taken place after 12 days in the field and greenhouse rainfall treatment. In the both test, the small seeded varieties were more tolerant than large or medium type. Also, the greenhouse rainfall treatment was compared with field rainfall treatment to evaluate the availability of greenhouse test. The result from the greenhouse rainfall treatment can represent the result of field test. Overall, these result showed that a rainfall after maturity significantly decreased seed quality. And greenhouse rainfall test for 12 days can be used to screen soybean varieties with resistant to field weathering, which can be used for development of field weathering resistant soybean variety.

국내 콩 유전자원의 Isoflavone 함량 변이

정명근,강성택,한원영,백인열,김현경,신두철,강남숙,황영선,안영남,임정대,김관수,박시형,김선림,Choung, Myoung-Gun,Kang, Sung-Taeg,Han, Won-Young,Baek, In-Youl,Kim, Hyeun-Kyeung,Shin, Doo-Chull,Kang, Nam-Suk,Hwang, Young-Sun,An, Young-Nam,Lim, 한국작물학회 2006 Korean journal of crop science Vol.51 No.suppl1

고 isoflavone 함유 콩 유전자원 선발 및 품종육성의 기초자료로 활용하기 위해 국내 콩 주요품종 43계통 및 보존 유전자원 648계통의 isoflavone 함량을 분석한 결과를 요약하면 다음과 같다. 1. 한국산 콩 주요 43품종의 평균 isoflavone 함량은 daidzein이 $484.4{\pm}223.9{\mu}g/g$, genisteinol $448.0{\pm}156.2{\mu}g/g$, glyciteinol $84.9{\pm}48.7{\mu}g/g$, total isoflavoneol $1,017.3{\pm}390.0{\mu}g/g$을 나타내었다. 2. 공시된 주요 품종의 isoflavone 함량은 석양풋콩이 $2,002{\mu}g/g$으로 가장 높은 함량을 나타내었고, 황금콩이 $277{\mu}g/g$으로 가장 낮은 함량을 나타내었다. 또한 콩 주요 43품종을 용도별로 분류하여 isoflavone 함량을 비교한 결과 풋콩 및 올콩이 $1,438{\pm}493.7{\mu}g/g$을 나타내어 조사된 용도별 콩 분류 중 가장 높은 양상을 나타내었다. 3. 국내 보존 콩 유전자원 648계통을 대상으로 isoflavone 함량을 조사한 결과 IT 180001계통이 $3,479{\mu}g/g$의 함량을 나타내어 가장 높은 양상을 나타내었고, IT 910878 계통은 $82.61{\mu}g/g$의 total isoflavone을 함유하여 가장 낮은 계통으로 조사되어 보존 유전자원간에 큰 함량변이를 나타내었다. Isoflavone in soybean is known to have the important physiological functions such as antioxidative, estrogenic, and anti-tumor activities. Therefore, isoflavone content is considered as important aspect of soybean quality. The objectives of this study are to determine the isoflavone contents of soybean germplasms including major cultivars in Korea, and to provide the basic information of isoflavone far development soybean cultivar containing high isoflavone content. Among 43 Korean major cultivated soybean samples, the total isoflavone contents were varied from $277.0{\mu}g/g\;to\;2,002.0{\mu}g/g$, and the average total isoflavone content was $1,017.3{\pm}390.0{\mu}g/g$. Total isoflavone content of Seokryangputkong $(2,002{\mu}g/g)$ was the highest among those of the other cultivated soybeans. On the other side, Hwangkeumkong $(277{\mu}g/g)$ contained lower isoflavone than other cultivars. The total isoflavone content of vegetable use or early maturity soybean cultivar $(1,438{\pm}493.7{\mu}g/g)$ showed more higher total isoflavone content than others. The daidzein, glycitein, genistein and total isoflavone contents of 648 soybean germplasms collected in Korea, were ranged $16.9{\sim}2,019.8{\mu}g/g,\;0{\sim}294.9{\mu}g/g,\;38.5\sim1,337.7{\mu}g/g,\;and\;82.6{\sim}3,479.2{\mu}g/g$, respectively, and total isoflavone content of IT 180001 line $(3,479.2{\mu}g/g)$ was the highest among those of other soybean germplasm lines.

콩 이모작 적응 조숙 다수성 품종 ‘황금올’

고종민(Jong Min Ko),백인열(In Youl Baek),한원영(Won Young Han),이병원(Byong Won Lee),이영훈(Young Hoon Lee),신상욱(Sang Uk Shin),강범규(Beom Kyu Kang),김현태(Hyun Tae Kim),하태정(Tae Joung Ha),문중경(Jung Kyung Moon),이석기(Seuk Ki L 한국육종학회 2016 한국육종학회지 Vol.48 No.3

A soybean cultivar for soy-paste, ‘Hwangkeumol’, was developed from the cross between SS92414 (crossing line of ‘Pokwangkong’ and ‘Suwon163’) and ‘Hwaeomputkong’ by soybean breeding team in the National Institute of Crop Science (NICS) in 2010. A promising line, SS99409-2B-11-5-4, was selected and designated as the name of ‘Milyang202’. It was prominent and had good result from regional adaptation yield trials for three years from 2008 to 2010 and released as the name of ‘Hwangkeumol’. It has a determinate growth habit, white flower, gray pubescence, yellow seed coat, yellow hilum, spherical seed shape and large seed (28.6 grams per 100 seeds). Maturity date of ‘Hwangkeumol’ was as early as September 15th. Therefore it is suitable for double cropping system. ‘Hwangkeumol’ was resistant to bacterial pustule and resistant to soybean mosaic virus, the major soybean diseases in Korea and tolerant to lodging in fields. The average yield of ‘Hwangkeumol’ was 2.51 ton per hectare in the regional yield trials carried out for three years from 2008 to 2010.