性敎育이 女高生의 性에 對한 知識 및 態度에 미친 效果

房美羅 韓國學校保健學會 1990 韓國學校保健學會誌 Vol.3 No.1

This study was attempted to assess the effects of a long term regular sex education on the knowledge about anatomy and physiology of human reproductive organg and the attitude toward sex of second grade high school girls. The study population included 1, 678 high school girls(542 educated second grade girls, 972 not educated second grade girls and 164 pre-educaled first grade girls ) attending in 3 high schools located in Kyungnam pro- The data was collected by guestionnaire to assess knowledge of sexual anatomy and physiology and the attitude toward sex from 21 to 24 December 1988. The results were as follows : Most of students ( educated group 99.3% , not educated group 98.1% ) agreed to necessity of sex education. Among students 70.8? did not talk about their sexual problems wish their parents and 30.1% of educated group obtained sexual knowledge from their teachers. There were significant differences between level of knowledge on anatomy and physiology of reproductive organs, secondary growth spurt, V D, pregnancy and contraceptives of educated group and not educated group (p < 0.01 ). There was significant difference between positive attitude about sexual intercourse before marriage, artificial abortion and family planning slogan of educated group and not educated group ( P < 0.05, p < 0.01 ). There were significant differences between degree of agreement about acquaintance with the other sex, knowledge of acquaintance with the other sex, talk freely wish friend, parent and teacher of educated group and not educated group ( P < 0.05, p < 0.01 ). Statistics revealed that high school girls of not educated group had less knowledge about acquaintance with the other sex than educated group ( P < 0.01 ).

예비 한국어 교사의 교사 효능감 변화 연구 - 교육 실습 과정을 중심으로 -

장미라 ( Jang Mira ),방성원 ( Bang Seong-won ) 연세대학교 언어연구교육원 한국어학당 2021 외국어로서의 한국어교육 Vol.61 No.-

The purpose of this study is to investigate the effect of the Korean teaching practicum on the teacher efficacy of pre-service Korean teachers. The participants were 190 students who took the Korean teaching practicum course at a Cyber University in Seoul. Data were collected three times: before the teaching practicum period, after class observation, and after the simulation class. The collected data were analyzed by frequency analysis, paired t-test, etc. The results of this study are as follows: First, pre-service Korean teachers have a high level of teacher efficacy when starting teaching practicum, and the teacher efficacy has significantly improved after completing the practicum. Second, more significant improvements were made after the simulation class during the teaching practicum period. Third, students with lower teacher efficacy were more influenced by class observation, and the teacher efficacy was greatly improved than those with higher teacher efficacy. Fourth, both students who performed class observation on video and students who additionally observed face-to-face classes improved their teacher efficacy after completing the teaching practicum. Based on the results of this study, we suggest implications for improving the teacher efficacy of pre-service teachers during the Korean teaching practicum. (Kyung Hee Cyber University)

性敎育이 女高生의 性에 對한 知識 및 態度에 미친 效果

정문숙,방미라 慶北大學校 醫科大學 1991 慶北醫大誌 Vol.32 No.2

본 조사는 인문계 여고생 2학년에게 연구자가 정규적으로 주1시간 총 24시간에 걸쳐 성교육을 지침서에 의거, 실시하고 1학년과 교육을 주지 않은 2학년 여고생과 비교하여 성에 대한 지식 및 태도에 준 효과를 설문지를 배부 후 회수하여 본 결과는 다음과 같다. 대상은 경남 지역 1개교 542명을 교육군으로, 1학년 164명 그리고 비교육군 2학년 972명을 대상으로 하였으며 설문기간은 1988년 12월 21일부터 23일까지 3일간이었다. 대상 여학생의 70.8%는 부모와 성문제를 상담하지 않았으며, 98.5%가 성교육이 필요하다고 응답하였다. 적절한 성교육 시기로 전체의 56.0%가 중학교라고 응답했으며 성지식의 출처로 선생님으로부터가 교육군의 30.1%인데 비해 비교육군은 19.1%로 양군간 유의한 차를 보였다(p<0.01). 성에 관련된 인간의 해부 생리 및 2차 성징, 성병, 피임 및 임신 생리 등의 지식의 정도를 1학년의 평균치를 기준으로 보았을 때 교육군은 평균치 이상인 여고생이 100.0%이었고 비교육군에 비해(54.3%, 76.3%, 75.1%) 유의하게 높은 비율이었다(p<0.01). 성에 대한 긍정적 태도, 혼전 성관계와 인공유산에 대한 바른 태도 및 가족계획 슬로건에 대한 긍정적 태도는 교육군과 비교육군간 찬성유무 및 생각해 본 일이 없다는 무관심 유무에서 유의한 차를 나타냈다(p<0.05, p<0.01). 고등학교 시절의 이성교제, 이성교제에 대한 지식, 친구, 부모, 선생님과의 자유로운 대화에 대한 찬성율이 교육군과 비교육군간 유의한 차(p<0.05, p<0.01)가 있었으며 생각해 본 일이 없다는 항목은 비교육군이 교육군보다 유의하게 높았다. This study was attempted to assess the effects of a long term regular sex education on the knowledge about anatomy and physiology of human reproductive organs and the attitude toward sex of second grade high school girls. The study population included 1,678 high school girls(542 educated second grade grils, 972 not educated second grade girls and 164 pre-educated first grade girls) attending in 3 high schools located in Kyungnam province. The data was collected by guestionnaire to assess knowledge of sexual anatomy and physiology and the attitude toward sex from 21 to 24 December 1988. The results were as follows: Most of students(educated group 99.3%, not educated group 98.1%) agreed to necessity of sex education. Among students 70.8% did not talk about their sexual problems with their parents and 30.1% of educated group obtained sexual knowledge from their teachers. There were significant differences between level of knowledge on anatomy and physiology of reproductive organs, secondary growth spurt, VD, pregnancy and contraceptives of educated group and not educated group(p<0.01). There was significant difference between positive attitude about sexual intercourse before marriage, artificial abortion and family planning slogan of educated group and not educated group(p<0.05, p<0.01). There were significant differences between degree of agreement about acquaintance with the other sex, knowledge of acquaintance with the other sex, talk freely with friend, parent and teacher of educated group and not educated group(p<0.05, 0<0.01). Statistics revealed that high school girls of not educated group had less knowledge about acquaintance with the other sex than educated group(p<0.01).

인간 배아줄기세포로부터 분화된 세포에서 MACS 방법을 이용하여 분리한 세포의 특성에 대한 연구

조재원,임천규,신미라,방경희,궁미경,전진현,Cho, Jae Won,Lim, Chun Kyu,Shin, Mi Ra,Bang, Kyoung Hee,Koong, Mi Kyoung,Jun, Jin Hyun 대한생식의학회 2006 Clinical and Experimental Reproductive Medicine Vol.33 No.3

목 적: 인간 배아줄기세포는 재생 의학이나 조직공학에 있어서 큰 잠재적인 능력을 가지고 있는 것으로 알려져 있다. 이들은 다양한 growth factors 처리나 유전자 발현을 변화시켜 특정 세포로 유도 분화 및 분리가 가능하지만 그 효율성은 아직까지 낮은 상태이다. 본 연구에서는 인간 배아줄기세포로부터 비특이적으로 분화된 세포들을 특정 세포 표면 항체를 이용한 magnetic cell sorting (MACS) 방법으로 분리, 배양하여 그들의 특성을 살펴보았다. 연구방법: 미분화 배아줄기세포주(Miz-hESC4)를 물리적인 방법으로 계대 배양하였으며, 부유 배양법으로 배아체 형성을 유도하였다. 배아체의 자발적인 분화를 위해 DMEM에 10% FBS를 첨가하여 2주 동안 배양하였다. 이렇게 분화된 세포들을 CD34, human epithelial antigen (HEA), human fibroblast (HFB)에 대한 항체를 이용한 MACS system으로 각각의 항체에 대한 양성 또는 음성 세포를 분리하였다. 이러한 MACS 분리 세포를 4주 동안 배양하면서 형태적인 변화를 관찰하고 특이 유전자의 발현 양상을 분석하였다. 결 과: 분리 배양한 CD34 양성 세포들은 배양 초기에는 둥근 형태를 나타내다가 배양 후기에는 작은 다각형의 형태로 관찰되었으며, HEA 양성 세포들은 큰 다각형의 형태를 나타내었고, HFB 양성 세포들은 전형적인 방추체 형태로 관찰되었다. 특이 유전자에 대한 RT-PCR 결과에서, CD34 양성 세포들과 HFB양성 세포들에서는 내배엽과 중배엽 관련 유전자의 발현하는 것을 확인할 수 있었고, HEA 양성 세포들에서는 외배엽 관련 유전자인 NESTIN과 NF68KD의 발현을 관찰할 수 있었다. 배양기간이 경과함에 따라 CD34 양성 세포의 특이 유전자 발현 양상이 변화되었다. 결 론: 이상의 결과는 비특이적으로 분화된 인간 배아줄기세포로부터 특이 세포를 MACS 방법을 이용하여 성공적으로 분리할 수 있음을 보여주었다. 따라서, MACS 방법과 특이 세포에 대한 항체는 인간 배아줄기세포의 유도 분화와 특이 세포의 분리에 매우 유용할 것으로 생각된다. Objective: Human embryonic stem (ES) cells have a great potential in regenerative medicine and tissue engineering. The human ES cells could be differentiated into specific cell types by treatments of growth factors and alterations of gene expressions. However, the efficacy of guided differentiation and isolation of specific cells are still low. In this study, we characterized isolated cells from differentiated human ES cells by magnetic activated cell sorting (MACS) system using specific antibodies to cell surface markers. Methods: The undifferentiated hES cells (Miz-hESC4) were sub-cultured by mechanical isolation of colonies and embryoid bodies were spontaneously differentiated with DMEM containing 10% FBS for 2 weeks. The differentiated cells were isolated to positive and negative cells with MACS system using CD34, human epithelial antigen (HEA) and human fibroblast (HFB) antibodies, respectively. Observation of morphological changes and analysis of marker genes expression were performed during further culture of MACS isolated cells for 4 weeks. Results: Morphology of the CD34 positive cells was firstly round, and then it was changed to small polygonal shape after further culture. The HEA positive cells showed large polygonal, and the HFB positive spindle shape. In RT-PCR analysis of marker genes, the CD34 and HFB positive cells expressed endodermal and mesodermal genes, and HEA positive cells expressed ectodermal genes such as NESTIN and NF68KD. The marker genes expression pattern of CD34 positive cells changed during the extension of culture time. Conclusion: Our results showed the possibility of successful isolation of specific cells by MACS system from undirected differentiated human ES cells. Thus, MACS system and marker antibodies for specific cell types might be useful for guided differentiation and isolation of specific cells from human ES cells.

비위험기구 소독제 사용 표준화를 통한 수행률 증진

손희정,정선영,주미탁,채남희,김숙희,김민자,방미라,이영숙 한국의료QA학회 2008 가을학술대회 Vol.2008 No.3