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효모의 자외선에 의한 절제회복 유전자 RAD4 의 크로닝
박상대,윤정교,최인순,허정호,최수명 한국유전학회 1985 Genes & Genomics Vol.7 No.3
The RAD 4 gene which is required for the incision step of UV-induced excision repair in Saccharomyces cerevisiae has been cloned. The recombinant DNA was constructed by introducing a partial Sau 3AI digest of the yeast chromosomal DAN into the Bam HI site of the cloning vector, YEp 24. After transforming the recombinant plasmid into the UV-sensitive mutant, a positive clone which showed a similar survival to the RAD^+ phenotype was isolated. This plasmied contained 6.54kb DNA fragment in addition to the original YEp 24. The DNA fragment was mapped by restriction analysis and the size of its gene product was determined.
포유동물세포에 있어 MMS 와 자외선에 의한 복제개시 및 DNA 생장억제
김영상,박상대,성노현,이치건,윤정교 한국유전학회 1983 Genes & Genomics Vol.5 No.3
Chinese hamster ovary (CHO) cells exposed to ultraviolet (UV)-light and methyl methanesulfonate (MMS), analysed by alkaline sucrose gradient sedimentation, showed an interruption of DNA replication. In UV-irradiated cells, dose-dependent response appeared; at low fluences below 1.42J/㎡ the inhibition of replicon initiation was caused and as the fluence increased the effect on replicon initiation was masked by the inhibitory effects on strand-elongation and joining. MMS also showed the inhibitory effects in dose dependent manner at both processes of replicon initiation and chain elongation of DNA. Treatment with UV and MMS concomitantly resulted in the additive inhibition effects of DNA replication.
Differential Response of Multiphasic Replication to UV - light
이치건,박수희,이명애,박상대 한국유전학회 1986 Genes & Genomics Vol.8 No.1
The nature of nascent DNA from both asynchronous and synchronous populations was investigated. In UV-irradiated synchronous cells, the amounts of nascent DNA, synthesized during the first 4hr in S phase, were increased to 1.5-fold. But this enhancing effect was not appeared during 5-10 hr of S phase. Asynchronous population also showed the increased level of DNA synthesis by UV-light. Effects of UV-light on the rate of DNA synthesis was somewhat different between asynchronous and synchronous populations. There's no apparent changes in synchronous population, whereas in asynchronous population the rate of DNA synthesis was greatly reduced and then gradually recovered to normal level thereafter. These results suggest that there are various classes of DNA, replicated at discrete times during S-phase and differently responding to UV-light.