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      • KCI등재

        반응표면분석법을 이용한 댕댕이 기능성성분의 마이크로웨이브추출조건 최적화

        박대희,이재준,박종진,박상환,이원영,Park, Daehee,Lee, Jae-Jun,Park, Jongjin,Park, Sanghwan,Lee, Wonyoung 한국식품저장유통학회 2017 한국식품저장유통학회지 Vol.24 No.5

        본 실험은 댕댕이의 마이크로웨이브 추출장치를 활용한 플라보노이드, 안토시아닌, 폴리페놀, 항산화능 등을 반응표면분석법을 통하여 최적추출조건의 예측과 추출조건에 따른 추출물의 기능성 성분 함량을 모니터링 하였다. 중심합성계획법을 통해 추출공정에 중요한 독립변수인 용액농도($X_1$: 0, 25, 50, 75, 100%), 추출시간($X_2$: 1, 4, 7, 10, 13 min), microwave power($X_3$: 180, 210, 240, 270, 300 W)를 각각 독립변수로 설정하였고, 총 플라보노이드 함량($Y_1$), 총 안토시아닌 함량($Y_2$), 총 폴리페놀 함량($Y_3$) 그리고 DPPH 라디칼 소거능($Y_4$) 등을 종속변수로 하여 회귀분석을 실시하였다. 예측된 최적조건 범위는 에탄올 농도 53-64%, 추출시간 7.4-7.9 min, microwave power 242-250 W로 나타났다. 플라보노이드 함량의 최대점은 에탄올의 농도 52.9%, 추출시간 7.5분, microwave power 244 W일 때 최대값은 65.74 mg RE/g으로 예측되었다. 안토시아닌 함량의 최대점은 에탄올의 농도 54.8%, 추출시간 7.4분, microwave power 248 W일 때 최대값은 0.45 mg CGE/g으로 예측되었다. 폴리페놀 함량의 최대점은 에탄올의 농도 56.9%, 추출시간 7.7분 및 microwave power 250 W 일 때 최대값은 14.71 mg GAE/g로 예측되었다. 항산화능의 최대점은 에탄올의 농도 64.10%, 추출시간 7.9분 및 microwave power 242 W일 때 최대값은 92.54%로 예측되었다. 예측값과 실험값의 차이가 거의 없는 것으로 보아 본 실험은 정확도가 높고 신뢰성이 높음을 확인할 수 있었다. 이와 같은 결과는 댕댕이 마이크로웨이브 추출방법에 있어서 최적의 추출조건에 대한 정보를 주는 것이며, 더 나아가 고농도 댕댕이 기능성 성분이 함유된 제품 개발에 기초정보를 제공하는 결과라 사료된다. Functional compounds including flavonoids, anthocyanins, polyphneols and antioxidants were extracted from blue honeysuckle (Lonicera caerulea L.) using highly efficient microwave-assisted extraction. And extraction process was modeled and optimized according to response surface methodology (RSM). The independent variables ($X_n$) were ethanol concentration ($X_1$: 0, 25, 50, 75, 100%), irradiation time ($X_2$: 1, 3, 5, 7, 9 min), and microwave power ($X_3$: 60, 120, 180, 240, 300 W). Dependent variables ($Y_n$) were total flavonoid contents ($Y_1$), total anthocyanin contents ($Y_2$), total polyphenol contents ($Y_3$) and antioxidant activity ($Y_4$). Four-dimensional response surface plots were generated based on the fitted second-order polynomial models to get optimal conditions. Estimated optimal conditions for 4 responses were ethanol concentration of 54-72%, irradiation time of 7.1-7.6 min, and microwave power of 243-251 W. Ridge analysis predicted the maximal responses of total flavonoid content, total anthocyanin content, total polyphenol content and antioxidant activity were 38.00 mg RE/g, 6.80 mg CGE/g, 14.90 mg GAE/g, 89.10%, respectively. Verification experiment was carried out at predicted optimal conditions and experimental values for total flavonoid content, total anthocyanin content, total polyphenol content and antioxidant activity were 38.10 mg RE/g, 6.72 mg CGE/g, 14.91 mg GAE/g and 89.13%, respectively. No significant difference was observed between predicted and experimental values, indicating good fitness of fitted model and successful application of RSM.

      • KCI등재

        암반대수층 지하수 인공함양 시험에 대한 열-수리 모델링

        박대희,구민호,김용철,Park, Daehee,Koo, Min-Ho,Kim, Yongcheol 한국지하수토양환경학회 2015 지하수토양환경 Vol.20 No.1

        An artificial recharge test aimed at investigating transport characteristics of the injected water plume in a fractured rock aquifer was conducted. The test used an injection well for injecting tap water whose temperature and electrical conductivity were different from the groundwater. Temporal and depth-wise variation of temperature and electrical conductivity was monitored in both the injection well and a nearby observation well. A highly permeable fracture zone acting as the major pathway of groundwater flow was distinctively revealed in the monitoring data. A finite element subsurface flow and transport simulator (FEFLOW) was used to investigate sensitivity of the transport process to associated aquifer parameters. Simulated results showed that aperture thickness of the fracture and the hydraulic gradient of groundwater highly affected spatio-temporal variation of temperature and electrical conductivity of the injected water plume. The study suggests that artificial recharge of colder water in a fractured rock aquifer could create a thermal plume persistent over a long period of time depending on hydro-thermal properties of the aquifer as well as the amount of injected water.

      • SCOPUSKCI등재

        치아발육시 방사선조사와 칼슘결핍이 골형성단백질-2/4의 분포에 미치는 영향에 관한 여구

        박대희,황의환,이상래,Park Dai-Hee,Hwang Eui-Hwan,Lee Sang-Rae 대한영상치의학회 2000 Imaging Science in Dentistry Vol.30 No.3

        Purpose: To investigate the expression of bone morphogenetic protein (BMP)-2/4 during eary tooth development after irradiation and calcium-deficient diet. Materials and Methods: The pregnant three-week-old Sprague-Dawley rats were used for the study. The control group was non-irradiation/normal diet group (Group 1), and the experimental groups were irradiation/normal diet group (Group 2) and irradiation/calcium-deficient diet group (Group 3). The abdomen of the rats at the 9th day of pregnancy were irradiated with single dose of 350 cGy. The rat pups were sacrificed at embryonic 18 days, 3 days and 14 days after delivery and the maxillae tooth germs were taken. The tissue sections of specimen were stained immunohisto-chemically with anti-BMP-2/4 antibody. Results: At embryo-18 days, immunoreacivity for BMP-2/4 of the Group 1 was modetate in stratum intermedium of dental organ and weak in dental papilla and dental follicle, but that of Group 2 was weak in cell layer of dental organ, and no immunoreacivity was shown in dental papilla and dental follice of Group 2 and in all tissue components of the Group 3. At postnatal-3 days, immunoreacivity for BMP-2/4 of the Group 1 was strong in cell layer of dental organ, odontoblasts and developing alveolar bone, but that of Group of 2 and Group 3 was weak in odontoblasts and developing alveolar bone. At postnatal-14 days, immunoreacivity for BMP-2/4 of the Group 1 was strong in newly formed cementum, alveolar bone and odontoblasts, but that of Group 2 was weaker than that of Group 1. In the Group 3, tooth forming cell layer showed weak immunoreactivity, but other cell layers showed no immunoreactivity. Couclusion : The expression of bone morphogenetic protein (BMP)-2/4 during early tooth development was disturbed after irradiation and calcium-deficient diet.

      • 제16회 세계검도선수권대회를 통해 본 한국국가대표팀과 일본국가대표팀의 경기패턴 분석

        박대희(Park, Dae-Hee),김영학(Kim, Young-Hak) 대한검도학회 2021 대한검도학회지 Vol.32 No.1

        이 연구의 목적은 제16회 세계검도선수권대회 당시 한국팀과 일본팀의 경기를 분석하여 각 팀의 경기패턴을 파악하고 이에 따른 강점과 약점을 찾아 보강·보완함으로써 이후 개최될 세계검도 선수권대회에서 대한민국의 우승을 위한 시사점을 제공하는 것이다. 이 연구를 수행하기 위해서 제16회 세계검도선수권대회 남자부 단체전 한국팀과 일본팀의 1회전부터 결승전까지 모든 경기를 경기분석 전용 소프트웨어인 스포츠 코드(Sports Code) V9.을 이용해 분석하였고, 프로그램 내의 매트릭스 시스템을 이용해 산출된 데이터를 계산하였다. 데이터의 신뢰도를 확보하기 위해 다른 연구자 2명(대한검도회 공인 4단 이상, 사범자격증 소지자)과 함께 각자 분석한 자료를 서로 비교, 대조해보는 방식인 삼각검증 방식을 채택하였다. 연구결과, 첫째, 한국팀의 공격 횟수는 일본팀보다 많았으며 상대적으로 한국팀은 머리치기 공격과 허리치기 공격의 비율이, 일본팀은 손목치기 공격과 찌름 공격의 비율이 높았다. 둘째, 일본팀의 머리치기 득점 비율이 한국팀보다 좀 더 높았으며 허리치기 공격의 득점 비율은 한국팀이 일본팀보다 더 높았다. 셋째, 한국팀과 일본팀 모두 전체 득점중의 대부분이 단타 공격에 의해 이루어졌다. 한국팀의 경우 일본팀보다 연타 공격의 비중이 더 컸음에도 불구하고 전체 득점 대비 연타 공격의 비율은 일본보다 낮았다. 넷째, 한국팀은 근거리 공격을 통한 득점 비율이 원거리공격보다 높았으며, 일본팀은 근거리 공격보다 원거리공격의 득점비중이 더 컸다. 끝으로 이 연구 결과에 대한 논의와 향후 연구를 위한 제언을 하였다. The purpose of this study was to provide implications to the Korea National Team for winning of the upcoming WKC. This study analyzed the match between the Korea National Team and the Japan National Team at the time of the 16th World Kendo Championships, identified the match patterns of each team, and found their strengths and weaknesses and reinforced and supplemented them. In order to conduct this study, all matches from the first round to the final between the Korea and Japan National teams in the men"s team event of the 16th World Kendo Championships were analyzed using Sports Code V9., a software dedicated to game analysis, and the matrix within the program. The data generated using the system were calculated. In order to secure the reliability of the data, a triangular verification method was adopted, which is a method of comparing and contrasting the data analyzed with two other researchers (4-Dan or higher, certified by the Korea Kumdo Association, holders of a Kumdo instructor"s license). The study results as follows: First, the number of attacks of the Korea National Team was higher than that of the Japan National Team, and relatively the Korean National Team had a higher ratio of head-slashing attacks (men) and waist-striking attacks (kote), and the Japan National Team had a higher ratio of wrist-slashing attacks (koto) and stabbing attacks (tuski). Second, the Japan National Team"s head-slashing attacks (men) scoring rate was slightly higher than that of the Korea National Team, and the Korea National Team"s back hit scoring (hiki men) rate was higher than the Japan National Team"s. Third, most of the total goals scored by both the Korea and Japan National Teams were made by single-hit attacks. In the case of the Korea National Team, the ratio of consecutive hitting to the total score was lower than that of Japan, although the proportion of consecutive hitting was larger than that of the Japan National Team. Fourth, the Korea National Team scored a higher percentage of goals from short-range attacks than from long-distance attacks, and the Japan National Team scored a higher percentage of goals from long-distance attacks than from short-range attacks. Finally, the results of this study were discussed and suggestions for future research were made.

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