방사선조사 후 유표피암종세포내 칼슘농도의 변화와 apoptosis 발현에 관한 연구

문제운,이삼선,허민석,최순철,박태원,유동수,Moon Je-Woon,Lee Sam-Sun,Heo Min-Suk,Choi Soon-Chul,Park Tae-Won,You Dong-Soo 대한영상치의학회 1999 Imaging Science in Dentistry Vol.29 No.1

Purpose: Ionizing radiations have been reported as an apoptosis initiating stimulus in various cells and it has established that sustained elevations in [Ca/sup 2+/] can lead to DNA fragmentation by Ca/sup 2+/-dependent endonucleases, ultimately resulting in apoptotic cell death. The previous experiments have been reported by using primarily thymocytes and lymphocytes and the change of [Ca/sup 2+/] was measured only by minutes or hours respectively. We need to evaluate [Ca/sup 2+/] in both several minutes and hours after irradiation of radiation of radiation therapy and verify the apoptotic cells. Materials and Methods: We have measured [Ca/sup 2+/] in human gingival epitheloid cancer cell with 10Gy irradiation, at minutely intervals and hourly intervals using digitized video-intensified fluorescence microscopy and the fluorescent Ca/sup 2+/ indicator dye, fura-2. In order to find out that the transient rise in [Ca/sup 2+/] could induced apoptosis, cells were incubated for 1 hour at 37℃ with TdT enzyme, rinsed and resuspended containing fluorescence and observed under a confocal fluorescence microscope. MTT assay was done to determine cell activity and LDH assay was done to determine the amount of necrotic cells. Results: After irradiation, the transient and temporal increasing of [Ca/sup 2+/] in the KB cells was founded. Though, there was no change in the intracellular [Ca/sup 2+/] at 30 minutes and 2 hours after irradiation. We could detect of DNA fragmented cells at 4 hours after 10Gy irradiated cells. There were no significant differences between 4 hour, 1 day, 3 day cells. There were no significant differences in MTT and LDH assay between the irradiated group and the control group after 4 hours and 1 day. Though after 3 days there were differences in MTT and LDH assay between the irradiated group was significantly decreased than the control group, in LDH assay the number of necrotic cell death of the irradiated was higher than the control group. Conclusion: In KB cells there were incipient and temporal increasing of the [Ca/sup 2+/] with 10Gy irradiation and the apoptosis was founded from 4 hours later which was earlier than seeing of the change of the amount of the cellular ability and necrosis.

파노라마 X선 사진을 이용한 무치악 환자의 방사선학적 연구

문제운,최순철 大韓口腔顎顔面 放射線學會 1992 Imaging Science in Dentistry Vol.22 No.2

The author has examined the abnormalities in panoramic radiographs of 668 edentulous patients. The distribution, type, incidence, and location of all abnomalities were analized, and the distances between alveoar crest and anatomical structures were measures. (Maxillary measurements were performed between alveloar crest and lower margin of the maxillary sinus and mandibular measurements between alveolar crest and upper edge of the mental foramen). The obtained results were as follows ; 1. Of the 668 panoramic radiographs examined, 103(15.4%) showed one or more abnormalities. 2. The incidence of abnormalities was decreased by years, which was 22.0% in 1970's and 16.8% in 1980's and 12.1% in 1990's. 3. The distribution of abnormalities as follows ; 43(40.2%) superficial root fragments, 24(22.4%) impacted teeth, 9(8.4%) imbedded root fragments, 7(6.5%) cysts, 7(6.5%) fractures, 5(4.7%) foreign bodies, 5(4.7%) abnormal radiolucencies, 4(3.7%) abnormal radiopacities, 2(1.9%) bony defects, 1(0.9) tumor. 4. In the location of abnormalities, 42 cases(35.3%) were in the maxillary posterior region, 26 cases(21.8%) were in the maxillary anterior region, 20 cases(24.4%) were in the mandibular posterior region and 18 cases(15.1%) were in the mandibular anterior region. 5. In the distance between alveolar crest and maxillary sinus, 109 cases(9.7%) were below 0.5mm, 757 cases(67.6%) were between 0.5mm and 10mm, 254 cases(22.7%) were above 100mm. In the distance between alveolar crest and mental foramen, 73 cases(8.8%) were below 0.5mm, 501 cases(60.7%) were between 0.5mm and 10mm and 252 cases(30.5) were above 100mm.

방사선조사 후 유표피암종세포내 칼슘농도의 변화와 apoptosis 발현에 관한 연구

이삼선,문제운,허민석,박태원,유동수,최순철 大韓口腔顎顔面 放射線學會 1999 Imaging Science in Dentistry Vol.29 No.1

Purpose: Ionizing radiations have been reported as an apoptosis initiating stimulus in various cells and it has established that sustained elevations in [Ca²+] can lead to DNA fragmentation by Ca²+-dependent endonucleases, ultimately resulting in apoptotic cell death. The previous experiments have been reported by using primarily thymocytes and lymphocytes and the change of [Ca²+] was measured only by minutes or hours respectively. We need to evaluate [Ca²+] in both several minutes and hours after irradiation of radiation of radiation therapy and verify the apoptotic cells. Materials and Methods: We have measured [Ca²+] in human gingival epitheloid cancer cell with 10 Gy irradiation, at minutely intervals and hourly intervals using digitized video-intensified fluorescence microscopy and the fluorescent Ca²+ indicator dye, fura-2. In order to find out that the transient rise in [Ca²+] could induced apoptosis, cells were incubated for 1 hour at 37℃ with TdT enzyme, rinsed and resuspended containing fluorescence and observed under a confocal fluorescence microscope. MTT assay was done to determine cell activity and LDH assay was done to determine the amount of necrotic cells. Results: After irradiation, the transient and temporal increasing of [Ca²+] in the KB cells was founded. Though, there was no change in the intracellular [Ca²+] at 30 minutes and 2 hours after irradiation. We could detect of DNA fragmented cells at 4 hours after 10 Gy irradiated cells. There were no significant differences between 4 hour, 1 day, 3 day cells. There were no significant differences in MTT and LDH assay between the irradiated group and the control group after 4 hours and 1 day. Though after 3 days there were differences in MTT and LDH assay between the irradiated group was significantly decreased than the control group, in LDH assay the number of necrotic cell death of the irradiated was higher than the control group. Conclusion: In KB cells there were incipient and temporal increasing of the [Ca²+] with 10 Gy irradiation and the apoptosis was founded from 4 hours later which was earlier than seeing of the change of the amount of the cellular ability and necrosis.(J Korean Oral Maxillofac Radiol 1999:29:109-117)

결합조직형성 법랑모세포종의 방사선학적 소견 : 증례보고 3례

최다혜,허경회,문제운,이원진,허민석,이삼선,최순철,박관수,Choi Da-Hye,Huh Kyung-Hoe,Moon Je-Woon,Yi Won-Jin,Heo Min-Suk,Lee Sam-Sun,Choi Soon-Chul,Park Kwan-Soo 대한영상치의학회 2006 Imaging Science in Dentistry Vol.36 No.1

Desmoplastic ameloblastoma is a rare histologic variant of ameloblastoma. It shows important differences in anatomic distribution, histologic appearance, and radiographic findings compared with the general type of ameloblastoma. It is histologically characterized by an abundance of densely collagenous stroma and radiographically a mixed radiolucent-radiopaque lesion. We present three cases of desmoplastic ameloblastoma. All the patients complained on buccal swelling with or without pain and the sites of occurrence were the anterior and the premolar region. Plain radiographs showed mixed radiopaque-radiolucent lesion with ill-defined or diffuse sclerotic margin and no external root resorption. Additionally, CT scans revealetl buccal expansion and relatively well-defined margin of the lesions. The clinical and radiographic features of the presented cases were compared with those of the desmoplastic ameloblastoma in the previous literatures.

결합조직형성 법랑모세포종의 방사선학적 소견 : 증례보고 3례

최다혜,허경회,문제운,이원진,허민석,이삼선,최순철,박관수 대한구강악안면방사선학회 2006 Imaging Science in Dentistry Vol.37 No.1

Desmoplastic ameloblastoma is a rare histologic variant of ameloblastoma. It shows important differences in anatomic distribution, histologic appearance, and radiographic findings compared with the general type of ameloblastoma. It is histologically characterized by an abundance of densely collagenous stroma and radiographically a mixed radiolucent-radiopaque lesion. We present three cases of desmoplastic ameloblastoma. All the patients complained on buccal swelling with or without pain and the sites of occurrence were the anterior and the premolar region. Plain radiographs showed mixed radiopaque-radiolucent lesion with ill-defined or diffuse sclerotic margin and no external root resorption. Additionally, CT scans revealed buccal expansion and relatively well-defined margin of the lesions. The clinical and radiographic features of the presented cases were compared with those of the desmoplastic ameloblastoma in the previous literatures. (Korean J Oral Maxillofac Radiol 2006; 36 : 63-8)

Three cases of cholesterol granuloma in the mandible

신민정,신재명,허경회,이원진,문제운,최순철 대한영상치의학회 2007 Imaging Science in Dentistry Vol.37 No.4

상악골 침범 소견을 동반한 선천성 침윤 지방종

허경회,이향옥,이서영,최형길,하병각,문제운,이삼선 대한구강악안면방사선학회 2007 Imaging Science in Dentistry Vol.37 No.2

Infiltrating lipoma is a rare mesenchymal neoplasm that, in spite of benign nature, characteristically infiltrates adjacent tissues and tends to recur after surgery. It has a predilection for the extremities and the trunk and is extremely rare in the head and neck region. We present a case of congenital infiltrating lipoma of the face, describing the intrabony invasion and osseous dystrophy as well as the soft tissue changes seen on plain radiographs and magnetic resonance imaging.