군리탕가감방(君理湯加減方)이 항종양(抗腫瘍) 면역반응(免疫反應)과 항암제로 유발(誘發)한 부작용(副作用)에 미치는 영향(影響)

유경태,문석재,문구,원진희,Yui, Guyng-Tea,Moon, Suk-Jae,Moon, Goo,Won, Jin-Hee 대한암한의학회 1998 大韓癌韓醫學會誌 Vol.4 No.1

Even though appropriate immune response is necessary for the survival of the individual, excessive or insufficient immune Response might cause autoimmune or allergic disease. So the immune response must be controlled to the degree that is beneficial for the well being of the individual. This study was undertaken to know the effects of Gunleetang Gagambang on the immune system of the mouse. Gunleetang Gagambang has been used for cure of tumor as a traditional medicine without any experimental evidence to support the rational basis for its clinical use. This study was carried out to evaluate the possible therapeutic or antitumoral effects of Gunleetang Gagambang extract against tumor, and to carry out some mechanisms responsible for its effect. Some kinds of tumor were induced by the typical application of 3-methylcholanthrene(MCA) or by the implantation(s.c) of malignant tumor cells such as leukemia cells(3LL cells) or sarcoma cells(S180 cells). Treatment of the Gunleetang Gagambang on water-extract(dailly 1mg/mouse, i. p.) was continued for 7 days prior to tumor induction and after that the treatment was lasted for 20 days. Against squamous cell carcinoma induced by MCA, Gunleetang Gagambang decreased not only the frequency of tumor production but also the number and the weight of tumors per tumor bearing mice(TBM). Gunleetang Gagambang on also significantly suppressed the development of 3LL cell and S180 cell-implanted tumors in occurrence-frequency and their size. and some developed tumors were regressed by the continuous treatment of Gunleetang Gagambang extract into TBM. In vitro, treatment of Gunleetang Gagambang extract had no effect on the growth of some kinds of cell line such as FsaII, A431 strain but significantly inhibited the proliferation of 3LL, S180 cells and augmented the DNA synthesis of mitogen-activated lymphocytes. Gunleetang Gagambang also stimulated the migrative ability of leukocyte, the MIF and IL-2 production of T lymphocytes, but not IL 6 production of B cells. Gunleetang Gagambang administration to mice enhanced NK cells activities. These results demonstrated that Gunleetang Gagambang extract exhibited a significant prophylactic benefits against tumors and its antitumor activity was manifested depending on the type of tumor cells. And these results also suggested that effect of Gunleetang Gagambang might be chiefly due to nonspecitie enhancement of NK cell activities and cell-mediated immune responses.

수치파두(修治巴豆) 및 파두가황연(巴豆加黃連)의 세포독성(細胞毒性)과 항종양(抗腫瘍) 효과(效果)에 관(關)한 실험적(實驗的) 연구(硏究)

조성각,문구,문석재,Jo, Soung-Gak,Moon, Goo,Moon, Suk-Jae 대한암한의학회 1995 大韓癌韓醫學會誌 Vol.1 No.1

This experiment was designed to study the change of cytotoxic and antitumor effects according to the prebrewed method of Semen Tiglii and Rhizoma Coptidis. The cytotoxic and antitumor effects were evaluated on human cell lines(A 549, Caki-1, LL2, Sarcoma 180, NIH/3T3) after exposure to prebrewed Semen Tiglii and Rhizoma Coptidis water extract 0.1, 0.2, 0.4, 0.8, 1.6 mg/ml using in MTT assay, LDH, colony forming efficency and SRB assay which were regarded as a valuable method for cytotoxic and antitumor effects of unknown compound on tumor cell lines. The results obtained in this studies were as follows. 1. The cytotoxicity from the result of MTT assay was low slightly in the ST II(炒巴豆霜), high in the ST III(醋炒巴豆). The cytotoxicity of ST I + RC(生巴豆霜加黃連) was similar to that of STI(生巴豆霜). 2. The cytotoxicity from the result of LDH was low slightly in the ST Ⅱ (炒巴豆霜), high in the ST III(醋炒巴豆). The cytotoxicity of ST I + RC(生巴豆霜加黃連) was similar to that of ST I(生巴豆霜). 3. The antitumor affect on A 549 tumor cell from the result of colony forming efficiency was low slightly in the ST II (炒巴豆霜) and ST I + RC(生巴豆霜加黃連). 4. The antitumor effect on Caki-1 tumor cell from the result of SRB assay was low slightly in the ST II (炒巴豆霜). 5. Median survival time and Increased life span increased slightly in the ST I RC(生巴豆霜加黃連) and ST II (炒巴豆霜). 6. The inhibitory effect on the growth of Sarcoma 180 and Lewis lung carcinoma tumor cell increased slightly in the ST I + RC(生巴豆霜加黃連) and ST II (炒巴豆霜).

진령탕가감방의 항종양효과(抗腫瘍效果)에 관(關)한 실험적(實驗的) 연구(硏究)

정준택,문구,문석재,Jeong, Jun-Tak,Moon, Goo,Moon, Suk-Jae 대한암한의학회 1998 大韓癌韓醫學會誌 Vol.4 No.1

과학기술(科學技術)의 발전(發展)에 따른 생활(生活) 및 의료(醫療) 수준의 향상(向上)에도 불구하고 산업발전(産業發展)에 따른 환경오염(環境汚染), 스트레스 증가(增加) 등 그 역기능(逆機能) 때문에 발암물질(發癌物質) 또는 자극(刺戟)에의 노출(露出) 등으로 인해서 전세계적(全世界的)으로 암(癌)으로 인(因)한 사망율(死亡率)이 증가(增加)되고 있다. 암(癌)은 현재(現在) 인류(人類)의 건강(健康)을 위협하는 주요(主要)한 질환(疾患)의 하나로, 오늘날 우리 나라에서 질환(疾患)으로 인(因)한 사망원인중(死亡原因中) 수위(首位)를 차지하고 있다. 이러한 암(癌)의 발생(發生)은 80%-90%가 환경적(環境的) 인자(因子)에 기인(起因)한다고 보고(報告)되고 있는데 앞으로 환경성(環境性) 발암물질(發癌物質)이 점차 증가(增加)됨에 따라 암환자(癌患者)의 발생(發生)도 더욱 증가(增加)될 것으로 예상(豫想)된다. 암(癌)의 서의학적(西醫學的) 치료(治療)는 주로 수술요법(手術療法), 방사선요법(放射線療法), 화학요법(化學療法) 및 면역요법(免疫療法) 등이 활용(活用)되고 있다. 이중 수술요법(手術療法)과 방사선요법(放射線療法)은 암(癌)이 전신(全身)에 전이(轉移) 증식(增殖)되기 전에 실시(實施)함으로써 효과(效果)를 거둘 수는 있으나, 암(癌)이 원발병소(原發病巢) 이외(以外)에까지 전이(轉移) 증식(增殖)되었을 경우에는 이 두가지 방법(方法)의 국소요법(局所療法)만으로서는 좋은 치료효과(治療效果)를 거두기 어렵다. 따라서 최근 항암제(抗癌劑)에 의한 화학요법(化學療法)이 다른 방법(方法)보다 유효(有效)하다고 인정(認定)이 되어 가장 많이 활용(活用)되고 있으나, 암종(癌種)에 대한 감수성차이(感受性差異), 치료후(治療後)의 부작용(副作用), 재발(再發) 및 합병증(合倂症) 등의 문제점(問題點)때문에 부작용(副作用)을 감소(減少)시키면서 항암효과(抗癌效果)를 높이는 치료법(治療法)이 계속 연구(硏究)되고 있다. 이에 1972년(年) 미국(美國) 국립암연구소(國立癌硏究所)에서 실시(實施)한 항암제(抗癌劑) 개발연구(開發硏究)에 있어서 항종양성(抗腫瘍性) Screening test에 이용(利用)된 물질중(物質中) 약 47%가 천연물질(天然物質)이었다는 사실은 주목(注目)할만 하다. 한의학적(韓醫學的) 치료법(治療法)은 최근(最近)에 변증시치(辨證施治)를 근간(根幹)으로 하여 건비익기(健脾益氣)를 위주(爲主)로한 부정거사법(扶正祛邪法)과 부정배본법(扶正培本法)이 많이 활용(活用)되고 있으며 부정법(扶正法)으로는 익기건비(益氣健脾), 자음보혈(滋陰補血), 온보비신(溫補裨腎) 등이, 거사법(祛邪法)으로는 활혈거어법(活血祛瘀法), 청열해독법(淸熱解毒法), 연견화담법(軟堅化痰法) 등이 활용(活用)되고 있는데, 이를 근거(根據)로 하여 한약(韓藥)의 복방(複方).단미(單味).단미추출물(單味抽出物) 등의 투여(投與)와 침(鍼) 구(灸) 수침(水鍼) 전침(電鍼) laser침(鍼)등의 치료방법(治療方法)을 수술(手術) 후(後) 혹(或)은 화학요법(化學療法)이나 방사선요법(放射線療法)와 병행(幷行)하여 치료효과(治療效果)가 향상(向上)되었다고 보고(報告)하고 있다. 항암제(抗癌劑)의 항암효과(抗癌效果) 정도(程度)를 검사(檢査)하는 방법(方法)은 크게 5가지로 분류(分類)되는데, 하나는 세포(細胞)를 약제(藥劑)에 노출(露出)시킨 후 현미경(顯微鏡)을 통하여 나타난 형태학적(形態學的) 변화(變化)를 가지고 약제(藥劑)의 효과정도(效果 The sprig of Jinryungtang Gagambang has been used for curing as a traditional medicine without any experimental evidence to support the rational basis for their clinical use. This experiment was carried out to evaluate the possible therapeutic or antitumoral effects of Jinryungtang Gagambang extract against cancer, and to study some mechanisms responsible for its effect. The cytotoxic and antitumor effects were evaluated on human cell liens (A549, hep3B, Caki-1, Sarcoma 180) after exposure to Jinryungtang Gagambang extract using in ILS, colony forming efficency and SRB assay which were regarded as a valuable method for cytotoxic and antitumor effects of unknown compound on tumor cell lines. The results obtained in this studies were as follows. 1. As a result of exposure to Jinryungtang Gagambang extract, the proliferation of A549, hep3B, Caki-1, good correlations were shown from the results of SRB assay and those of clogenetic assay. 2. The oral administration of Jinryungtang Gagambang extract showed significant effects of increase of MST(mean survival time) and ILS(increased life span) depending on the increasing concentration. 3. Against squamous cell carcinoma induced by MCA, Jinryungtang Gagambang decreased not only the frequency of tumor production but also the number and weight of tumors per tumor bearing mice(TBM). Jinryungtang Gagambang also significantly suppressed the development of 3LL cell-implanted tumors by frequency and their size, and some developed tumors were regressed by the continuous treatment of Jinryungtang Gagambang extract into TBM. 4. Jinryungtang Gagambang extract also increased NK cell activities. According to the above results, it could be suggested that Jinryungtang Gagambang extract has prominent antiutmor effect.

백화사설초 메탄올 추출물에 의한 HL-60 세포(細胞) 고사과정(枯死過程)에서의 cell cycle 관련인자(關聯因子)의 활성변화(活性變化) 연구(硏究)

한세희,이종범,문구,문석재,원진희,박래길,이종덕,Han, Se-Hee,Lee, Jong-Bum,Moon, Gu,Moon, Suk-Jae,Won, Jin-Hee,Park, Lae-Gil,Lee, Jong-Deok 대한암한의학회 2000 大韓癌韓醫學會誌 Vol.6 No.1

Objectives: Hedyotis diffusa is used to treat cancer in traditional Korea Medicine. So this study was carried out to examine the expression of cell cycle related genes in HL-60 cells undergoing apoptosis by the methanol extract of Hedyotis diffusa. Methods: 1. HL-60 cells were treated with various concentrations (from 200 to $50{\mu}g/ml$)of methanol extract and H20 extract ($200{\mu}g/ml$) of hedyotis diffusa. After 48 h later, the cells were tested for viability by MTT assay. 2. The HL-60 cells were treated with $200{\mu}g/ml$ of methanol extract for the indicated periods. The whole cell lysates were prepared and analyzed by western blotting using anti-p53 antibody. 3. The nuclear extract were prepared and analyzed by western blotting using anti-p21 antibody, anti-p27 antibody, anti-cyclin A antibody, anti-cyclin E antibody and anti-CDK2 antibody. Results: 1. The methanol extract of Hedyotis diffusa induced the death of HL-60 cells in a dose dependent manner. 2. The methanol extract of Hedyotis diffusa makedly decreased the level of p21/Cipl and cyclin A in a time dependent manner. 3. The methanol extract of Hedyotis diffusa markedly increased the level of p27/Kipl and cyclin E in a time dependent manner. 4. The methanol extract of Hedyotis diffusa markedly did not affect the level of CDK2. Conclusions: These results provide evidence that expression of cell cycle related genes in HL-60 cells undergoing apoptosis by the methanol extract of Hedyotis diffusa mainly results from decreased level of p21/Cipl and increased level of p27/Kipl of the cell cycle related genes.

백화사설초 메탄올 추출물에 의한 HL-60 세포고사과정에 있어서의 transcriptional factors 활성변화 연구

박상구,이지현,문구,문석재,원진희,박래길,Park, Sang-Goo,Lee, Ji-Hyun,Moon, Gu,Moon, Suk-Jae,Won, Jin-Hee,Park, Lae-Gil 대한암한의학회 2000 大韓癌韓醫學會誌 Vol.6 No.1

Objective : Hedyotis diffusa has been used as an anticancer agent for several decades in oriental medicine. We test whether the methanol extract of the herb affects transcriptional activation factors including $NF-{\kappa}B$ and AP-1. Methods : 1. HL-60 cells were treated with various concentrations(from 200 to $50{\mu}g/ml$) of methanol extract and $H_2O$ extract($200{\mu}g/ml$)of hedyotis diffusa, After 48h later, the cells were tested for viability by MTT assay. 2. The HL-60 cells were treated with $200{\mu}g/ml$ of methanol extract for the indicated periods. First. Nuclear extracts were isolated and incubated with oligonucleotide probe of $NF-{\kappa}B$ and AP-1. Second. Nuclear extracts were isolated and reacted with p50, p65. c-rel pan-Jun, c-Jun, JunB. JunD antibody on ice for 30min. Finally The cell lysates were prepared and analyzed by western blotting using anti-Fas, anti-FasL and anti-p53 antibody. Results : 1. The methanol extract decreases the viability of human lymphoid origin leukemia HL-60 cells in a dose-dependent manner. 2. $NF-{\kappa}B$ is rapidly activated by the addition of the methanol extract, reaches a peak at 30min and gradually returns to resting level. We confirm that $NF-{\kappa}B$ is a heterodimer mainly composed of p65 subunit with c-Rel. 3. Transcriptional activation of AP-1 is detected at 30min and reaches a maximum at 1hr after stimulation of the cells with the methanol extract. AP-1 is mainly composed with Jur-D and partially Jug-B proteins. 4. the methanol extract of Hedyotis diffusa induces the expression of Fas, Fas ligand and p53 proteins of HL-60 cells in a time dependent fashion. Conclusions : These results suggest that the methanol extract of Hedyotis diffusa exerts anticancer effects to induce the death of human leukomic HL-60 cells via activation of trascriptional factors such as $NF-{\kappa}B$ and AP-1, increase in expression of Fas mediated signalling proteins, and induction of tumor suppressor gene. p53.

Cisplatin을 처리한 뇌세포에서 補血免疫丹의 세포방어효과

강태희(Tai Hee Kang),문구(Gu Moon),문석재(Suk Jae Moon),원진희(Jin Hee Won) 한의병리학회 2002 동의생리병리학회지 Vol.16 No.2

肛腸療法에 의한 玉屛風散加味의 卽刻型 알레르기 반응 억제 효과

조정연(Jeong Yeon Cho),문구(Gu Moon),문석재(Suk Jae Moon),원진희(Jin Hee Won),유경태(Kyoung Tae You),이종덕(Jong Deok Lee) 한의병리학회 2002 동의생리병리학회지 Vol.16 No.2

고강도 사이알론의 균열치유와 고온강도 특성

남기우(Ki Woo Nam),박상현(Sang Hyun Park),박승원(Seung Won Park),문석재(Suk Jae Moon) 대한기계학회 2009 大韓機械學會論文集A Vol.33 No.9

Sialon was produced by hot-pressing the mixtures of Si₃N₄, AlN and Y₂O₃ powders. All fracture tests were performed on a three-point loading system with a 30 ㎜ bending span. Fracture toughness and Vickers hardness of smooth specimen were average 7.05 ㎫ m<SUP>0.5</SUP> and Hv = 1580, respectively. Density of three kinds of specimens, smooth specimen, smooth and healed specimen, smooth with SiO₂ colloidal coating and healed specimen, had beyond 99 % of theoretical density. Bending strength of smooth healed specimens had high strength more than 1 ㎬. Crack healed specimens recovered as strength as smooth specimen. That is, cracked specimen with SiO₂ colloidal coating on cracked part recovered strength by heat treatment, completely. Crack healing of Si₃N₄ composite ceramics had contributed glassy SiO₂ to strength recovery. Limiting high temperature for bending strength of heat treated smooth specimen for bending strength was about 1273 K.

고강도 사이알론의 균열치유와 고온강도 특성

남기우(Ki Woo Nam),박상현(Sang Hyun Park),박승원(Seung Won Park),문석재(Suk Jae Moon) 대한기계학회 2009 대한기계학회 춘추학술대회 Vol.2009 No.5

Sialon was produced by hot-pressing the mixtures of Si₃N₄, AIN and Y₂O₃ powders. All fracture tests were performed on a three-point loading system with a 30 ㎜ bending span. Fracture toughness and Vickers hardness of smooth specimen were average 7.05 ㎫ㆍm<SUP>0.5</SUP> and Hv = 1580, respectively. Density of three kinds of specimens, smooth specimen, smooth and healed specimen, smooth with SiO₂ colloidal coating and healed specimen, had beyond 99 % of theoretical density. Bending strength of smooth healed specimens had high strength more than 1 ㎬. Crack healed specimens recovered as strength as smooth specimen. That is, cracked specimen with SiO₂ colloidal coating on cracked part recovered strength by heat treatment, completely. Crack healing of Si₃N₄ composite ceramics had contributed glassy SiO₂ and crystal Y₂Al₂O? to strength recovery. Limiting high temperature for bending strength of heat treated 'smooth specimen for bending strength was about 1273 K.

MAEMS : 멀티 에이전트 기반 응급 의료 시스템 모델링

노선택(Seon-Taek Noh),이근상(Keun-Sang Lee),문석재(Suk-Jae Moon),엄영현(Yuong-Hyun Eum),정계동(Kye-Dong Jung),최영근(Young-Keun Choi) 한국정보과학회 2007 한국정보과학회 학술발표논문집 Vol.34 No.1D

응급환자의 생존 가능성은 응급의료체계가 얼마나 잘 갖추어져 있느냐에 따라 결정된다. 과거의 응급체계가 가장 빠른 시간에 환자의 이송함으로서 치료를 받게 하는데 초점을 두었다면 최근은 RFID등의 기술을 이용하여 환자의 상태를 빨리 파악하여 최적의 치료를 받을 수 있는 병원으로 이송할 수 있는 시스템 도입에 초점을 두고 있다. 따라서 응급 환자의 기본적인 정보를 수집한 후 최적의 병원 전 단계(pre-hospital phase)를 수립하기 위해서는 다양하고 이질적인 정보 자원들을 접근할 수 있어야 한다. 또한 응급환자의 기본적인 사고정보를 가지고 최적의 치료를 받을 수 있는 정책을 수립하기 위해 자율적으로 서로 커뮤니케이션을 할 수 있는 시스템이 필요하다. 본 논문에서는 멀티에이전트 기반의 응급 의료 시스템 모델링을 제시하고, 응급환자에 대한 최적의 병원 전 단계를 수립하기 위한 시퀀스를 설명한다.