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Karen S. Katula,Johnette A. McCain,Anthony T. Radewicz 한국식품영양과학회 2005 Journal of medicinal food Vol.8 No.2
The ability of various dietary compounds to modulate the activity of the transcription factor nuclear factor .B(NF-.B) was examined using a cell-based reporter system. NF-.B is central to the response of cells to stress and has beenlinked to cancer. HCT 116 (human colon carcinoma) and HepG2 (human liver carcinoma) cell lines were stably transfectedwith a NF-.B luciferase reporter vector. The reporter cell lines were preincubated with different concentrations (050 .M)of ascorbic acid, epigallocatechin gallate, genistein, quercetin, naringenin, and resveratrol for varying periods of times (112hours), after which the NF-.B inducer tumor necrosis factor-. (TNF-.) was added (48 ng/mL) for 4 hours. Compound alone,without TNF-., did not alter luciferase activity. Levels of TNF-.-induced luciferase (NF-.B) activity varied depending oncompound type and concentration, whereas preincubation time and cell type contributed less. Significant changes in luciferase(NF-.B) activity were detected for some of the compounds at more physiological concentrations (110 .M). Our data sug-gest that dietary modulation of NF-.B activity involves distinct mechanisms, depending on compound type and concentra-tion. More generally, this approach can be utilized for analyzing dietary compounds for effects on specific cellular factorsover a range of concentrations and incubation times, in combination, and in different cell types.