Ultra-fast and universal detection of Gram-negative bacteria in complex samples based on colistin derivatives

류제성,정현정 한국공업화학회 2021 한국공업화학회 연구논문 초록집 Vol.2021 No.0

Ubiquitous Detection of Gram-negative Bacteria Using Colistin as the Targeting Ligand

류제성,임산해,정현정 한국공업화학회 2019 한국공업화학회 연구논문 초록집 Vol.2019 No.0

The rapid and differential detection of Gram-negative infections would greatly reduce the overuse of antibiotics, and minimize the spread of antibiotic resistance. However, conventional diagnostics relies on microbial culture which is time-consuming, while molecular methods involve complex processing procedures. Herein, we studied the use of antibiotics as a targeting ligand for ubiquitous detection of Gram-negative bacteria. We chose colistin as the targeting ligand, which includes cationic residues (L-α-γ-diaminobutyric acid) that specifically interact with lipopolysaccharide (LPS) on Gram-negative bacteria. We prepared Cy3-conjugated colistin which were added to different types of pathogenic bacteria for incubation. Selective labeling of Gram-negative bacteria was demonstrated by confocal microscopy and spectrofluorometry. Our study presents a rapid and simple labeling procedure, which can be applied in the clinic for diagnosing Gram-negative infections.

Rapid Labeling of Gram-Negative Bacteria Using Colistin as a Ubiquitous Targeting Ligand

류제성,정현정 한국공업화학회 2018 한국공업화학회 연구논문 초록집 Vol.2018 No.0

Gram-negative bacteria is a serious cause of infections, which have to be rapidly and accurately diagnosed for specific and early treatment. Because conventional molecular tests based on antibodies or oligonucleotide probes, have limitations in their non-specific binding, cross-reactivity, and difficult access due to large molecular size, rapid labeling on bacterial cell wall is difficult to achieve. In order to differentiate Gram-negative bacteria from Gram-positive bacteria, we investigate on colistin (polymyxin E) due to its molecular attaching properties on lipopolysaccharide, known as the cell wall components of Gram-negative bacteria. Herein, we synthesized colisitn-Cy3 to labeling and detecting Gram-negative bacteria rapidly and accurately. In 1 hour labeling and washing process, we successfully confirmed colistin-Cy3 and Gram-negative bacteria interaction, and quantified that this bacteria makes stronger fluorescent labeling than others with fluorescent reader, microscope.

1P-218 Rapid detection of Gram-negative bacteria using Colistin as a ubiquitous targeting ligand

류제성,정현정 한국공업화학회 2017 한국공업화학회 연구논문 초록집 Vol.2017 No.1

Gram-negative bacteria is a serious cause of infections, which have to be rapidly diagnosed for specific and early treatment. Conventional methods include microbial culture, immunoassays, and nucleic acid testing, however have limitations in involving time-consuming procedures, lack of specific ligands, or expensive equipments. Colistin bind specifically to the lipopolysaccharide on the cell wall of Gram-negative bacteria. Herein, we apply Colistin as the ligand to ubiquitously target Gram-negative bacteria for rapid detection with high selectivity. We prepared colistin modified with Cy3, to rapidly label and visualize Gram-negative bacteria. Labeling of the bacteria with colistin-Cy3 was done by incubation for 10 min, and we successfully observe bright fluorescence on Gram-negative bacteria by microscopy, as well as strong signals by quantification. This labeling method can be used as a rapid and simple molecular detection platform for ubiquitous detection of Gram-negative pathogens.

공안사범자료관리의 법적 문제점에 대한 검토

류제성 법과사회이론학회 2012 법과 사회 Vol.0 No.43

This research examines the current condition of the data on public safety offenders, which is computerized and managed according to the Provision on the Data of Public Safety Offenders, a presidential directive, and assesses the legal issues concerning the Provision with the aim of proposing reform measures. The state has carried out a broad range of surveillance on people who have opposed or criticized the government and, based on these surveillance, has indiscriminately collected sensitive private information. This information has been computerized and kept on file for use by the government. However, this kind of collecting of private information has no legal basis, and the subject of the information cannot exercise any right as he is not aware that his records are classified as public safety offense records. This hinders the rule of law and constitutes a serious human rights violation. Moreover, the current method of managing public safety offenders’ data constitutes a head-on violation of the Private Information Protection Act which imposes various restrictions as to the collection and use of private information. The scope of public safety offenses based on the Provision on the Management of Data of Public Safety Offenders is too large and allows the possibility of arbitrary expansion by the government. Also, adding to the recently reinforced public safety functions, the Provisions guarantee the initiative of the prosecutor’s office regarding the management of the data of public safety offenders, raising concerns of abuse of authority and human rights violation on the part of the prosecutor’s office. Although the Provisions have been amended to slightly limit the scope of public safety offenses, fundamental corrections are yet to take place. Above all, current conditions of the management of public safety offenders’data must be disclosed, and then the necessity to collect the information of public safety offenders must be assessed. If, as a result of the assessment,the collection of information is deemed necessary, appropriate acts must be established so that the scope, subject, requirements, etc of information collection is limited in a clear and specific manner and that the rights of the subject of the information is guaranteed. This is an inevitable demand of our constitution.

2P-253 Rapid Fluorescent Labeling of Gram-Negative Bacteria Using Colistin-Functionalized Nanoprobes

류제성,정현정 한국공업화학회 2017 한국공업화학회 연구논문 초록집 Vol.2017 No.0

2. 횡적 부조화를 동반한 골격성 III급 부정교합 환자의 수술교정 치험례

류제성,황충주 연세대학교 치과대학 두개안면기형연구소 2016 연세임상교정 Vol.23 No.-

사용자 인터페이스의 새로운 길 #1

류제성(Jae-Sung Ryu) 한국디자인학회 2000 디자인학연구 Vol.- No.37

피의자의 수사기록 열람 · 등사권의 헌법적 권리성

류제성(Ryu Je-Sung) 국민대학교 법학연구소 2015 법학논총 Vol.28 No.2

2007. 6. 1. 개정된 형사소송법상 피고인 · 변호인의 수사기록 열람 · 등사권은 헌법상 신속 · 공정한 재판을 받을 권리, 변호인의 조력을 받을 권리, 무죄추정원칙 및 법적 청문청구권 등으로부터 파생하는 기본권이다. 그런데 형사소송법상 수사기록 열람 · 등사의 시기가 공소제기 이후로 제한되어 있어 현행법상 피의자에게는 수사기록 열람 · 등사권이 인정되지 않는다고 해석할 소지가 있다. 그러나 헌법적 형사소송법론에 입각하여 볼 때 피의자의 수사기록 열람 · 등사권 인정 여부는 형사소송법 명문규정의 유무가 아니라 피의자의 방어권 보장이라는 헌법적 가치가 기준이 되어야 한다. 오늘날 수사절차는 단순히 공판절차의 준비단계가 아니라 사실상 공판절차를 좌우할 만큼 독자적인 중요성을 지니고 있다는 점에서 피의자의 방어권을 피고인의 방어권보다 소홀하게 취급할 수 없다. 그리고 피의자의 방어권 보장은 곧 피고인의 방어권 보장과 직결된다는 점에서 양자를 구별하는 것은 합리적이지 않다. 따라서 형사소송법상 명문규정이 없더라도 헌법 해석상 신속 · 공정한 재판을 받을 권리, 변호인의 조력을 받을 권리, 무죄추정원칙 및 법적 청문청구권의 한 내용으로 피의자의 수사기록 열람 · 등사권은 당연히 인정된다고 보아야 할 것이다. According to the Criminal Procedure Act revised on June 1, 2007, a criminal defendant and his or her attorney’s right to read and copy the record of criminal investigation is a part of the basic human rights derived from the constitutional right to have a speedy and fair trial, the right to counsel, the presumption of innocence and the legal hearing right. By the way, the period for reading and copying the record of investigation prescribed in the criminal procedure law is limited to after the prosecution, it can be interpreted that the current law does not recognizing the accused person’s right to read and copy the record of investigation. From the perspective of the constitutional criminal procedural law theory, however, the recognition of an accused person’s right to read and copy the record of investigation shall be based on the constitutional value of guaranteeing the defendant’s defence right as opposed to the existence of a substantive stipulation in the Criminal Procedure Act. Considering the fact that the current investigation procedure is not just a preparation step before the trial procedure but bears independent significance for it actually influences the trial procedure, an accused person’s defence right cannot be treated more negligently than a criminal defendant’s defence right. Furthermore, it is not rational to differentiate accused person and criminal defendant, since guaranteeing an accused person’s defence right is directly connected to guaranteeing a criminal defendant’s defence right. So although there is not substantive stipulation in the Criminal Procedure Act, by the interpretation of constitution, the accused person’s right to read and copy the record of investigation should be recognized as the part of constitutional right to have a speedy and fair trial, the right to counsel, the presumption of innocence and the legal hearing right.

공직선거법 제93조 제1항 한정위헌결정(2007헌마1001)의 의미와 과제

류제성(Ryu Je-Seong) 부산대학교 법학연구소 2012 법학연구 Vol.53 No.1

이번 결정은 심판대상이 인터넷상의 정치적 표현의 자유에 한정되었고 법원이 기속력을 인정하지 않는 한정위헌결정이라는 한계가 있다. 그러나 이번 결정은 인터넷상 정치적 표현의 자유의 중요성과 대의민주주의에서 선거의 가치를 인정하면서 엄격한 심사기준을 적용하였다는데 그 의의가 있다. 헌재는 이번 결정에서 보여준 전향적인 입장을 오프라인상의 선거운동의 자유를 제한하는 다른 규정에 대한 심사에서도 관철할 필요가 있다. 인터넷 매체의 특성을 감안할 때 인터넷상 표현의 자유가 보다 더 보장되는 것이 타당하지만, 오프라인상의 정치적 표현도 그에 못지않은 중요성을 지니기 때문이다. 다양한 선거운동방법이 허용되어야 일반유권자들의 선거참여가 활성화되어 선거가 민주적 대의기관 구성이라는 본연의 목적을 달성할 수 있다. 그리고 중앙선거관리위원회와 검찰은 이번 헌법재판소 결정을 존중하여 인터넷상 정치적 표현 내지 선거운동을 단속하거나 기소하는 것을 삼가야 한다. 법원은 설령 검찰이 기소하더라도 무죄판결을 선고하여야 한다. 그리고 판례를 변경하여 이번 결정에 근거한 재심청구를 인용하여 이번 결정으로 실질적인 권리구제가 가능하도록 해야 한다. 근본적으로 국회는 이번 결정을 계기로 인터넷상의 선거운동 기간제한을 없앰과 아울러 오프라인상의 선거운동의 자유 제한에 대해서도 개폐를 본격적으로 논의해야 할 것이다. This decision exerts limited impact because the subject of the ruling was limited to the freedom of political expression on the Internet and the Court found a circumstantial violation of the constitution, which is not acknowledged by the Court to have an effect of sentence. However, the significance of this ruling is that the Constitutional Court, in the process of delivering it, applied the strict assessment standard and acknowledged the importance of freedom of political expression on the Internet and the value of election in a society of representational democracy. The Constitutional Court should maintain the same progressive stance it showed in this decision when deliberating on other provisions restricting the freedom of offline political expression or election campaign. While it is reasonable, considering the characteristics of Internet media, that freedom of expression on the Internet is better protected, offline political expressions are no less important. A diversity in means of running election campaigns should be permitted in order to encourage participation by voters and to achieve the objective of forming a democratic representational body. Furthermore, the National Election Commission and the Prosecutor's Office must respect the Constitutional Court's decision and refrain from cracking down on or prosecuting online political expressions or election campaigns. The Court must acquit the convicted even when the Prosecutor's Office decides to prosecute. It should change its precedent so as to allow petition for retrial based on the decision at hand, therefore making it possible for a substantial remedy for violation of right. Fundamentally, the National Assembly should take this opportunity to eliminate the limitation of duration for online election campaigns, and it should begin a full-scale discussion on the make and break of the restrictions on offline election campaigns.