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류시환 ( Si Hwan Ryu ),최재근 ( Jae Keun Choi ),김문종 ( Moon Jong Kim ),한정헌 ( Jeong Heon Han ),왕승현 ( Seung Hyun Wang ),김희연 ( Hee Yeon Kim ),김기선 ( Ki Sun Kim ),남궁민 ( Min Namgung ),박종열 ( Jong Yeol Park ),박기진 ( 강원대학교 농업생명과학연구원 2022 강원 농업생명환경연구 Vol.34 No.0
The main procedures of maize breeding are inbred line development and hybrid selection. Inbred line development is an essential procedure for the creation of good hybrids, but in South Korea, inbred line development is completely dependent on conventional breeding methods. This conventional inbred line development system is a tedious and time-consuming procedure. Doubled haploid technology in maize is a rapid inbred line development method and many foreign maize research institutes are actively using this technology. In this study, we aimed to set up and utilize doubled haploid technology in our breeding program. Inducer lines, which can produce haploid seeds, are a prerequisite for doubled haploid breeding. The Maize Research Institute secured the right to use tropically adapted haploid inducer lines (TAILs). The number of days to silking for TAILs was 72 and the flowering time was appropriate for crossing with other populations. When 48 maize populations were crossed with the inducer, the average haploid induction rate was 6.0%. Haploid induction rate for 2014 through 2017 were 3.9, 4.8, 6.7, and 8.3%, respectively. When we compare the chromosome doubling stage, the inbred line production rate of seedlings was higher than that of germinated seeds. Agronomic characteristics of inbred lines developed by doubled haploid technology were tested and those lines will be good resources as parent plants for making hybrids. Future work will aim to increase induction rate and the selection efficiency of haploid seeds, but our breeding efficiency and capacity in South Korea will be greatly improved by this technology.
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국내육성 팝콘옥수수 팝핑부피 향상을 위한 적정 수확시기
최재근 ( Jae-keun Choi ),류시환 ( Si-hwan Ryu ),김희연 ( Hee Yeon Kim ),김문종 ( Moon-jong Kim ),한정헌 ( Jeong-heon Han ),왕승현 ( Seung Hyun Wang ),김기선 ( Ki Sun Kim ),사규진 ( Kyu Jin Sa ),이주경 ( Ju Kyong Lee ) 강원대학교 농업생명과학연구원 2022 강원 농업생명환경연구 Vol.34 No.0
국내육성 팝콘옥수수 팝핑율 향상을 위하여 ‘오륜팝콘’, ‘오륜2호’, ‘지팝콘’, ‘기찬팝콘’에 대해 출사 후 수확시기별 팝핑율을 비교하였다. 국내육성 팝콘옥수수의 생육특성은 출사일수는 78-81일 사이로 나타났으며, 착수고율은 53.4-64%로 다소 높게 나타났다. 출사 후 등숙 기간에 따른 이삭특성은 등숙 기간이 길수록 이삭장은 짧아지고 백립중은 증가하였다. 팝핑부피는 ‘오륜팝콘’과 ‘오륜2호’ 품종에서는 출사 후 40일 후 측정에서는 각각 29.8 ± 0.2 cm<sup>3</sup>/g, 30.4 ± 0.1 cm<sup>3</sup>/g로 낮게 나타났으나 70일 후 수확에서는 34.8 cm<sup>3</sup>/g, 35.8 ± 0.1 cm<sup>3</sup>/g로 높게 나타나 두 품종의 수확 적기는 출사 후 70일로 나타났다. ‘지팝콘’과 ‘기찬팝콘’은 출사 후 40일 28.6 cm<sup>3</sup>/g, 34.3 ± 0.1 cm<sup>3</sup>/g로 나타났으나 60일 후 팝콘부피가 35.2 ± 0.1 cm<sup>3</sup>/g, 35.9 ± 0.1 cm<sup>3</sup>/g 증가하여 최적 수확기는 출사 후 60일로 나타났다. 수확기에 따른 일반성분 분석에서는 등숙 기간이 길수록 수분함량이 감소하였으며, 탄수화물, 단백질 함량은 증가하였다. 조지방 함량은 3.0 g/100 g대로 나타났다. 따라서, 국내육성 팝콘옥수수의 수확시기는 출사 후 60-70일이 적정하며 팝콘의 팝핑부피 품질을 높일 수 있는 최적 수확시기라고 판단된다. In this study, we investigated the effects of harvest time on the popping volumes of “Oryun popcorn,” “Oryun #2,” “G popcorn,” and “Kichan Popcorn” to improve the popping quality of domestic maize. The silking days of the four popcorn maize varieties ranged from 78-81 d and their plant-ear height ratio was 53.4-64%, which is rather high. The ear lengths were shorter and the grain weights were greater at 70 days after silking (DAS) than at 40 DAS. For the “Oryun Popcorn” and “Oryun #2” varieties, the popping volumes measured at 40 DAS were 29.8 ± 0.2 cm<sup>3</sup>/g and 30.4 ± 0.1 cm<sup>3</sup>/g, respectively, and at 70 DAS were 34.8 ± 0.0 cm3/g and 35.8 ± 0.1 cm<sup>3</sup>/g, respectively. The ideal harvest time for both varieties was 70 DAS. The popping volumes of “G Popcorn” and “Kichan popcorn” varieties evaluated at 40 DAS was 28.6 cm<sup>3</sup>/g and 34.3 ± 0.1 cm<sup>3</sup>/g, respectively. The optimal harvest time for these varieties was 60 DAS, when their popping volumes were 35.2 ± 01 cm3/g and 35.9 ± 0.1 cm<sup>3</sup>/g, respectively. Analysis of the general composition of the harvest showed that the longer the ripening period, the lower the moisture content and the higher the carbohydrate and protein content. The crude fat content was less than 3.0 g/100 g. Therefore, the optimal harvest time for domestically grown popcorn maize that maximizes the popping volume for improved popcorn quality was 60-70 DAS.
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김희연(Hee-Yeon Kim),박종열(Jong-Yeol Park),박기진(Ki-Jin Park),류시환(Si-Hwan Ryu),장은하(Eun-Ha Chang),고병대(Byeong-Dae Goh),윤병성(Byeong Sung Yoon),용우식(Woo Sik Yong),최재근(Jae-Keun Choi) 한국육종학회 2021 한국육종학회지 Vol.53 No.4
A new anthocyanin-rich hybrid variety, ‘Saekso 1’, is developed which is characterized by yellow grains, and purple husks andcobs. This variety was produced by crossing two inbred lines, ‘HA1’ (as the seed parent) and ‘HA2’ (as the pollen parent). It was madein 2008/2009 and evaluated in Hongcheon for two years. After evaluation, the selected variety was named ‘Saekso 1’ and was approvedfor a variety registration in 2014. The anthocyanin content of ‘Saekso 1’ in husk was 10.39±0.09 g/100 g, which was much higher thanthat of Kangilok (0 mg/100 g). Since regional tests were conducted only in Gangwon province, this variety is recommended only in thatregion for commercial cultivation. ‘Saekso 1’ is a health food resource for bioactive materials (Registration No. 4967).
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형광영상을 이용한 옥수수 반수체 종자 선발 효율성 향상
김영욱(Younguk Kim),한정헌(Jeong Heon Han),김재영(Jaeyoung Kim),김영태(Yeongtae Kim),김년희(Nyunhee Kim),이채원(Chaewon Lee),이서연(Seoyeoun Lee),김송림(Song Lim Kim),김문종(Moon Jong Kim),류시환(Si Hwan Ryu),이홍로(Hongro Lee),지현소(H 한국육종학회 2022 한국육종학회지 Vol.54 No.4
Many studies concerning breeding maize varieties are in progress in Korea and other countries. Double haploid technology is widely used for the development of commercial maize varieties worldwide, and has also been utilized in Korea since its introduction by the Maize Research Institute, Gangwondo. We performed a study to improve the efficiency of selecting haploid maize seeds using fluorescence imaging. It was verified that anthocyanin produced by the expression of R1-nj gene can be detected by fluorescence imaging, and we developed a high-throughput method for discriminating between haploid and diploid seeds. Compared with discriminating with naked eye, this method reduced the time for discriminating haploid and diploid maize by 91.7% and increased selection accuracy by 16.8% for haploid and 2.2% for diploid maize. This method enabled the acquisition of more haploid seeds and improved the efficiency of breeding research by shortening the time involved.
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