목본식물의 화학적 성분 분석과 구성성분과의 관계

김학곤 ( Hak Gon Kim ),송현진 ( Hyun Jin Song ),서영롱 ( Young Long Seo ),임현정 ( Hyun Jeong Im ),조호준 ( Ho Jun Jo ),양우형 ( Woo Hyeong Yang ),양재경 ( Jae Kyung Yang ),최명석 ( Myung Suk Choi ) 한국임학회 2014 산림과학 공동학술대회 논문집 Vol.2014 No.-

국내 바이오매스의 활용도와 효율성을 높이기 위해 산림청 조림권장수종을 포함한 86종의 산림자원에 대하여 화학적 성분을 조사하였다. 각 수종은 NREL의 분석방법으로 저분자화합물, lignin, hemicellulose, cellulose의 함량을 조사하였다. 또한 이들 화학적 조성성분간의 상관관계에 대하여 SPSS 대응표본 T검정을 통하여 조사하였다. 그 결과 국내 자생 목본 수종의 화학적 성분은 매우 큰 차이를 보였다. Extractives의 함량이 가장 높았던 종은 가막살나무였고, lignin의 함량이 가장 높았던 종은 명자나무였다. Hemicellulose 함량이 가장 높았던 종은 서양측백 이였고, 낮은 종은 단풍나무였다. Cellulose의 함량이 가장 높았던 종은 벚나무였다. Lignin의 함량이 높을수록 헤미셀룰로오스와 셀룰로오스의 함량은 감소하는 경향을 보였다. 본 연구 결과는 조림권장수종에 대한 이용성 증대에 기여할 것으로 판단된다. In order to increase the efficiency and utilization of domestically producible biomass, wood chemical component for 86 woody plants was analyzed. Each tree species were collected, mecerated and then quantifiable the content of extractives, lignin, hemicellulose, cellulose using the method of NREL. Further, it was statistical analysed through SPSS corresponding sample T-test for correlation between chemical compositions. As a result, the chemical composition of domestic woody species showed a huge variable according to plant species and chemical component. Among woody plants, extractive content was highest in Viburnum dilatatum and lignin content was high contained in c haenomeles lagenaria. The hemicellulose content was highly contained in Thuja occidentalis, however a cer palmatum was lowest compared to other species. Cellulose was highly contained in Prunus serrulata. Statistically, we have found that the higher lignin content is, the more it cellulose content. These results will do much to alleviate of utilization of Korean native woody plants.

Extending the Storage Periods of Zanthoxylum schinifolium Seed Oil using Sodium Bicarbonate and Ascorbic Acid

Hak Gon Kim(김학곤),Seung Mi Kang(강승미),Seong Hyeon Yong(용성현),Yu Won Seol(설유원),Do Hyeon Kim(김도현),Jun Ho Park(박준호),Chan Yeol Yu(유찬열),Myung Suk Choi(최명석) 한국약용작물학회 2020 한국약용작물학회지 Vol.28 No.6

Changes in the Physicochemical Characteristics of Sancho Oil according to the Purification Process

강승미,김학곤,양우형,용성현,박동진,박준호,최명석,Kang, Seung Mi,Kim, Hak Gon,Yang, Woo Hyeong,Yong, Seong Hyeon,Park, Dong Jin,Park, Jun Ho,Enukwa, Ettagbor Hans,Choi, Myung Suk The Korean Society of Medicinal Crop Science 2017 한국약용작물학회지 Vol.25 No.5

Background: Sancho oil extracted from Zanthoxylum schinifolium (Siebold & Zucc) is a useful edible oil that has been in use for a long time, but it is known to be susceptible to rancidity. Sancho oil purification can remove impurities to prevent rancidity. This study was performed in order to improve the quality of sancho oil and enhance its availability throughout the purification process. Methods and Results: Sancho oil extracted in Hadong, Korea was refined via the degumming and deoxygenation processes, following which we examined the changes in the polyphenol content, fatty acid content and antioxidant activity of the oil. Acetic acid was effective for deoxygenation of sancho oil and 2 N NaOH was effective for its deoxidation. The polyphenol content and antioxidant activity were reduced by the purification process. Saturated fatty acids contents did not vary with the degumming and deoxygenation processes, however the content of unsaturated fatty acids were slightly reduced. Conclusions: This study suggests that the process of sancho oil purification used in this study will contribute to the increased use and storage of sancho oil.

2년생 버드나무종의 바이오매스 생산 및 화학적 조성

송현진 ( Hyun Jin Song ),김학곤 ( Hak Gon Kim ),서영롱 ( Yeong Rong Seo ),임현정 ( Hyeon Jeong Im ),조호준 ( Ho Jun Cho ),양우형 ( Woo Yheong Yang ),정미진 ( Mi Jin Jeong ),심선정 ( Seon Jeong Sim ),최명석 ( Myung Suk Choi ) 한국임학회 2014 산림과학 공동학술대회 논문집 Vol.2014 No.-

해외에너지 자원의 의존도 감소를 위하여 유럽에서는 단기임업(SRC)이 빠르게 확산되고 있다. 본 연구에서는 11종의 버드나무를 대상으로 바이오매스 생산 및 화학적 조성을 조사하였다. 버드나무속 식물은 2011년 3월 경상대학교 학술림 시험지에 식재한 후 2011년 11월과 2012년 11월에 수확하였다. 공시수종들은 수고, 직경, 생중량 및 건중량을 측정하였다. 그 결과 가장 수고가 높은 수종은 Salix 1 이였고, 가장 낮은 수종은 Salix12 이었다. 전체적으로 바이오매스량은 Salix1 종이 가장 높게 나타났다. 수종간 화학적 조성분 또한 다르게 나타났다. 저분자물질인 Extractive 함량이 가장 높았던 종은 Salix 2였고, 가장 낮은 종은 Salix3 이였다. 리그닌 함량이 가장 높았던 종은 Salix1 12 였고, 가장 낮은 종은 Salix9 였다. Hemicellulose의 함량이 가장 높았던 종은 Salix9 였고, 가장 낮은 종은 Salix1 였다. Cellulose의 함량이 가장 높았던 종은 Salix5였고, 가장 낮은 종은 Salix7 이였다. 이상의 결과는 한국산 바이오매스종의 개발에 기여할 것으로 판단된다. Short rotation foresty (SRF) for energy purposes is rapidly expanding in Europe because of the reduced dependence on foreign sources of energy. This study was determined eleven Salix species biomasss production and chemical composition. The Salixspecies were planted in March 2011 at Gyeongsang National University Experiment Forest, harvested in November 2011and again in November 2012. All trees were measured(diameter, tree height, stemwood fresh weight and dry weight). As a result, the highest tree length species was S. glandulosa, and lowest species was S. gracilistyla ``golden`` Overall, the amount of biomass was highest S. glandulosa. The composition of constituents can vary different one plant species to another. Low-molecular material extractive content was the highest species of S. gracilistyla ``golden``, lowest species was S. sachalinensis ``Sekka``. The highest of lignin content was S. gracilistyla ``golden`` and lowest was S. alba ``white``, Hemicellulose content was found the highest species of S. alba ``white``, S. glandulosa has the lowest species Cellulose content was highest species was S. gracilistyla, S. viminalis gigantea has the lowest species. These results were contribute to the development of Korea biomass species

수분결핍에 따른 식물의 생리적 변화와 내건성 자생식물종 선발에 관한 연구

임현정 ( Hyeon Jeong Im ),송현진 ( Hyun Jin Song ),김학곤 ( Hak Gon Kim ),서영롱 ( Yeong Rong Seo ),조호준 ( Ho Jun Cho ),양우형 ( Woo Hyeong Yang ),정미진 ( Mi Jin Jeong ),마호섭 ( Ho Seop Ma ),최명석 ( Myung Suk Choi ) 한국임학회 2014 산림과학 공동학술대회 논문집 Vol.2014 No.-

식물은 건조 등의 스트레스 하에 놓여 있고, 수분을 효율적으로 이용하는 내건성 식물의 선발은 biomass 생산과 종보존을 위해 매우 중요하다. 본 연구는 식물의 내건성 생리적지표를 이용하여 내건성 식물을 선발하였다. 자생식물 110종 중 발아율이 높은 수종인 26종을 공시수종으로 선발했다. 내건성을 조사하기 위해 균일한 식물체에 무관수 처리하여 고사 소요일을 조사하였다. 식물의 수분스트레스에 비례하여 증가하는 프롤린 함량을 분석하여 내건성 평가지표로 활용하였다. 수분결핍에 따른 고사소요일을 조사한 결과, 2일째부터 고사하기 시작하여 10일 이후에도 생존하였다. 그 결과 새, 도깨비가지, 긴담배풀, 매듭풀, 비수리, 참느릅나무는 내건성 수종으로 입증되었다. Proline 분석 결과, proline 함량은 선발된 내건성 식물은 건조조건 부여 전과 내건성 민감성 수종보다 높았다. 본 연구는 내건성 식물종의 육종에 크게 기여할 것으로 판단된다. Many plants are stressed by prolonged periods of dry weather. Selecting trees that use water efficiently must be important in a future plant biomass and conservation. This study was selected of drought tolerance plant by using physiological indicators for drought tolerance. 110 plants germinated and highly germinating 26 species were selected. Four weeks old plants were stopped to irrigation to find out withering day by moisture deficiency. The proline content of the water stressed leaf tissues was analyzed and withering day according to moisture deficiency was examined. When stopped to irrigation, whithering day of plant species was variable from 2 to 10 days. Drought resistance plant confirmed 6 species as Ulmus parvifolia, Lespedeza cuneata, Kummerowia striata, Carpesium divaricatum, Solanum carolinense, and Arundinella hirta Proline content in selected drought tolerance plant was significantly increased than before stopped irrigation and drought susceptible plants. These results can be served breeding of drought tolerance plant from Korean native plants.

노거수 및 보호수 왕버들 삽목증식에 미치는 상토의 영향

송현진 ( Hyun Jin Song ),정미진 ( Mi Jin Jeong ),김학곤 ( Hak Gon Kim ),서영롱 ( Young Rong Seo ),임현정 ( Hyeon Jeong Im ),양우형 ( Yang Woo Hyeong ),박동진 ( Park Dong Jin ),윤석락 ( Yun Seok Lak ),마호섭 ( Ho Seop Ma ),최명석 ( 한국산림과학회 2015 한국산림과학회지 Vol.104 No.1

Old growth and protected-trees are worth history and culturally in Korea. This research carried out to investigate habitat characterization and the vegetative propagation methods of Salix chaenomeloides(100-600 years old trees) in Gyeongsangnamdo Province. It has been preserved in good and grow naturally in area surrounding village(4 trees), riverside(1 tree) and field(1 tree). Cutting from old growth and protected trees was conducted using 10 kinds of bedsoils. It was survived above 93% in all bedsoils. Growth of cuttings is a distinct difference according to bedsoils and tree ages. Growth of cutting was high on CBS, followed mixed bedsoils (VPMP) including vermiculite, peatmoss and perlite. Root collar diameter was difference depend on tree ages and bedsoils. All trees are produced 1 to 4 branch. Root growth of cutting was 20 cm on CBS and VPMP, which is the best shoot growing on cutting. Bedsoil with physico-chemical characteristics of high moisturizing ability, high air permeability, high EC value and slightly acid may be suitable for efficiently cutting propagation of old growth and protected S. chaenomeloides. CBS and VPMP bedsoil are favorable for propagating this species by cuttings.

자생지 우점수종의 산마늘 발아 및 유묘 생장에 미치는 잠재적 알렐로파시 효과

정미진 ( Mi Jin Jeong ),송현진 ( Hyun Jin Song ),김학곤 ( Hak Gon Kim ),서영롱 ( Yeong Rong Seo ),임현정 ( Hyeon Jeong Im ),서강욱 ( Gang Uk Suh ),최명석 ( Myung Suk Choi ) 한국임학회 2014 산림과학 공동학술대회 논문집 Vol.2014 No.-

알렐로파시 효과 구명을 위해 수종은 울릉도 산마늘 자생지의 우점종인 너도밤나무, 우산고로쇠를 내륙지역 산마늘 자생지 우점종인 신갈나무, 그리고 내륙지역 산지에 가장 많이 분포하는 소나무와 잣나무에 대해 산마늘 종자발아와 실생묘의 생장에 미치는 영향을 조사하였다. 각 수종의 잎을 산마늘종자의 발아율 및 생장을 측정하기 위해 낙엽처리, 분쇄잎 처리, 그리고 냉·열수 추출물을 각각 처리하였다. 그 결과, 낙엽처리에서는 53~58%로 상대적으로 발아율이 높았고, 분쇄된 잎을 인공토와 섞어처리하였을 때 산마늘의 발아력이 가장 낮게 나타났다. 수종 중에서는 소나무 처리구가 가장 산마늘의 발아율을 억제시켰으며, 지상부 생장에도 부정적인 영향을 주었다. 냉·열수 추출물을 다른 농도로 처리하였을 때 5% 소나무 추출물이 산마늘 종자의 발아를 가장 억제하였고, 전체적으로 냉수추출에서는 침엽수인 소나무와 잣나무 처리에서 낮은 발아를 보였다. 자생지 우점종인 너도밤나무 처리는 산마늘종자의 발아와 실생묘 생장에 큰 영향을 주지 않았지만, 열수추출된 활엽수 추출물은 산마늘의 종자발아와 실생묘 생장에 부정적인 영향을 주었다. Allelopathic effect of a dominant tree species on A. victorialis var. platyphyllum habitat were investigated using fallen, grinded leaves and aqueous extracts. Fallen leaves showed germination percentage in 53~58%. Seed germination on grinded leaves was inhibited significantly compared to fallen leaves feeding. Fallen leaves of Pinus densiflora were significantly inhibited on seedling growth compared to other tree species. Root growth of seedling was inhibited in 3g treatment of Acer okamotoanum, P. densiflora and Pinus koraiensis fallen leaves. P. densiflora and P. koraiensis in inland area inhibited the seed germination and seedling growth which indicates that plants may possible allelopathic activity. Cold and hot aqueous extracts of 5 tree species caused a reduction in germination percent, seedling vigour index. Tolerance index was significantly low as 5.0% in 5% extract of P. densiflora and exhibited unique pattern on seed germination according to deciduous or conifer tree. Cold aqueous extracts of the P. densiflora revealed the strong inhibitory effect on germination, with hot aqueous extracts of deciduous trees exhibiting lower germination percentage.