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Aspergillus niger CAD 1 의 부분 정제된 β- Galactosidase 에 의한 갈락토올리고당의 생성
김창렬(C . R . Kim),이석래(S . R . Lee),이용규(Y . K . Lee) 한국축산학회 1990 한국축산학회지 Vol.32 No.6
This study has attempted to seek the efficient way for the formation of galactooligosaccharides by the partially purified β-galactosidase from Aspergillus niger CAD 1. The results are summarized as follows; In the case of ion exchange purification step, the purification fold and recovery of β-galactosidase from A, niger CAD 1 were 8.4 and 24.8%, respectively. The overall yield of the partially purified enzyme was 6.3% of acetone fraction and the final enzyme preparation was purified 48.07 times. After the final purification step of Sephadex G-100 gel filtration, the purification fold and recovery of enzyme were 48.07 and 6.3% respectively. The molecular weight of the purified β-galactosidase after Sephadex G-100 gel filtration was estimated to be 106,000 by SDS-PAGE. The rate of oligosaccharide formation during hydrolysis of lactose was influenced by the concentration of lactose and enzyme, reaction time, and temperature. When cheese whey containing 20% lactose was treated with the partially purified β-galactosidase(2.0 units/ml) at 45℃ for 4hrs, the maximum conversion of lactose to oligosaccharides was about 18.9% by weight of total lactose. TLC, HPLC, and paper chromatogram of hydrolyzates in cheese whey containing 20% lactose by the partially purified β-galactosidase showed the formation of 1,3 and 8 of galactooligosaccharides, respectively.