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산란 후기 사료 내 거미(Nephila clavata) 유래 단백질 분해효소제(Arazyme<SUP>Ⓡ</SUP>)의 첨가 급여가 난 생산성에 미치는 영향
김정언(J. U. Kim),김재영(J. Y. Kim),김지숙(J. S. Kim),이보근(B. K. Lee),이소연(S. Y. Lee),이완섭(W. S. Lee),유선종(S. J. You),안병기(B. K. Ahn),김은집(E. J. Kim),박호용(H. Y. Park),손광희(K. H. Son),신동하(D. H. Shin),강창원(C. W. Kang) 한국가금학회 2009 韓國家禽學會誌 Vol.36 No.1
가계분석에 의한 k- casein 과 β- lactoglobulin 의 유전자형 분리에 관한 연구
이광전(K . J . Lee),김정언(J . U . Kim),이연근(Y . K . Lee),강민식(M . S . Kang),강석형(S . H . Kang) 한국축산학회 1995 한국축산학회지 Vol.37 No.3
This study was performed to offer basic data of both reducing generation interval and improving selection accuracy of dairy cattle for application of parentage test according to analyzing loci of κ-casein and β-lactoglobulin in related Korean dairy cattle using the PCR. The blood samples used in this study were taken from 24 related dairy cattle in Sin-Jung dairy farm and the frozen semen of two straws used in this study were taken from commercial firms. The results obtained were as follows; 1. This study established the extracting method of high quality DNA from the blood samples of 24 related dairy cattle and from two frozen semen samples by using phenol and determined the analyzing method for the polymorphism of κ-casein and β-lactoglobulin using PCR. 2. According to the restricted enzyme digestion in amplified products of κ-casein and β-lactoglobulin using PCR, κ-casein showed AA and AB types, and β-lactoglobulin showed AA, AB, and BB types, respectively. 3. By restricting enzyme digestion, genotypes of κ-caaein and β-lactoglobulin for dairy cows were always derived from genotypes of one parent or both parents. 4. Genetic panems between κ-casein and β-lactoglobulin markers showed independent gene segregations, therefore, in case of being known sire genotypes, genotypes of daughters would be detemuned by probability. 5. For ttte genetic improvement of dairy cattle, if the semen of sire with κ-casein BB or β-lactoglobulin BB were used by the A.I, genotyping method of marker genes can be useful for early identification and selection of dairy calves.
PCR 기법을 이용한 한국내 젖소의 k- casein 과 β- lactoglobulin 유전자형 분석에 관한 연구
이광전(K . J . Lee),김정언(J . U . Kim),이연근(Y . K . Lee),홍경표(K . P . Hong),김경수(K . S . Kim) 한국축산학회 1995 한국축산학회지 Vol.37 No.4
This study was performed to offer rapid and accurate methods of both sire selection and dairy cattle improvement, according to analyzing loci of κ-casein and β-lactaglobulin in Korean dairy cattle. The blood samples used in this study were taken from the 45 Holstein cattle in the nearby Kyung gi province, where milk was sold to Kon-Kuk milk plant. The results obtained were as follows; 1. This study established the extracting method of high quality DNA from the 45 Holsteins blood samples by using phenol and the analysis of κ-casein and β-lactoglobulin polymotphisms by using PCR. 2. Due to the amplification of κ-casein and β-lactoglobulin loci by using PCR, this study confirmed amplified products of 8746p and 961bp, respectively. 3. Due to the restricted enzyme digestion of Taq I and Hind Ill in amplified products of κ-casein, AA type with Hind III and Taq I showed no change of size in amplified products and BB type with Hind III showed 525bp and 350bp fragments and with Taq I showed 558bp and 317bp fragments, respectively. 4. Due to the restricted enzyme digestion of Hph I in amplified products of β-lactoglobulin, all lanes which were digested by restriction enzyme showed 961bp and 741bp fragments and A.4 type showed 220bp, while AB type showed 220bp and 166bp, and BB type showed 166bp fragments, respectively. 5. The κ-casein genotype of Holstein by restricted enzyme digestion gives frequencies of 75.55%, 17.78%, and 6.67%r for AA, AB, and BB types and for A and B alleles being 0.84 and 0.16, respectively. Restriction enzyme digestion of the β-lactoglobulin genotype of Holstein gives frequencies of 34.38%, 43.74%, and 21.88% for AAA, AB, and BB types and for A and B alleles being 0.56 and 0.44, respectively. 6. The fitness test(x²-test) for genotype frequencies of κ-casein and β-lactoglobulin showed gene equilibrium with AA. AB and BB, and β-lactoglobulin indicated Hardy -Weinberg equilibrium in the ratio of being 1:2:1. 7. The genotyping of κ-casein and β-lactoglobulin loci can be utilized for finding the association between genotypes and production traits in dairy cattle.
폐쇄 핵 젖소집단에서 IVEP수준과 육종계회게 의한 선발반응과 근친계수
이광전,김정언,강민식,조광현,장길원,최현준 한국동물자원과학회 2000 한국축산학회지 Vol.42 No.2
This study was carried out for the aim of solving the selection response on nucleus herds in dairy cattle by using the Multiple Ovulation Embryo Transfer(MOET), coupled with adapting In Vitro Embryo Production(IVEP), and settled down the nine kinds of hierachical mating plans according to the number of sires and dams, and did simulation for breeding schemes and IVEP levels. The results obtained were as follows ; 1. The annual rate of inbreeding coefficients were decreased according to increasing the number of sires and dams. It was low in the mating plan with many sires and dams, when the mating ratios of dam to sire are equal. These trends were similar to the juvenile, adult and progeny testing schemes. 2. The annual rate of selection response was high as increasing IVEP levels and the number of sires and dams. 3. The annual rate of selection response was the highest in the case of adult scheme and was the lowest in the case of progeny test scheme, regardless of mating plans and IVEP levels. 4. The rate of selection responses in the low IVEP level with many sires and dams were similar to those in the high IVEP level with few sires and dams, so if we would increase IVEP levels, the number of sires and dams and the herd sizes would be decreased, this practice would be advantageous for making nucleus dairy herds. 5. In cult scheme with medium IVEP level, it was expected that the cumulative selection response amounted to one and more standard deviation units in 10 years and to two standard deviation units in 20 years.