조선시대 미라 뇌 조직을 이용한 질병 관련 유전자 진단에서 Multiplex Ligation Dependent Probe Amplification Assay 기법 적용 가능성 검토

김유수(You Soo Kim),오창석(Chang Seok Oh),홍종하(Jong Ha Hong),성문우(Moon-Woo Seong),신동훈(Dong Hoon Shin) 대한체질인류학회 2015 해부·생물인류학 (Anat Biol Anthropol) Vol.28 No.3

Multiplex Ligation dependent Probe Amplification (MLPA) 기법은 거대 결실/중복을 확인하여 다양한 질병을 진단하는 도구로 임상의학 영역에서 폭넓게 이용되고 있다. 이 기법은 소량의 DNA만 있어도 분석이 가능하고 실험이 간단한 장점이 있어 고고학 현장에서 얻어진 옛 사람 시료 연구에 유리한 점이 있을 것이라 판단된다. 하지만 실제로 이 기법을 체질인류학 연구에 성공적으로 적용할 수 있을 지에 대해서는 관련 정보가 충분치 않아 아직 확신할 수 없다. 본 연구는 이에 조선시대 미라에서 얻어진 시료에 현재 가장 많이 상업적으로 가장 많이 쓰이는 MLPA 기법을 적용하여 객관적으로 관련된 정보를 얻고자 하였다. 이 연구에서는 실험이 시도된 여러 미라 시료 중 단 한 개체 (HD2)에서만 분석을 시도할만한 결과를 얻었지만 이에 대해서도 완전히 신뢰할 수 있는 수준이라고 판정하기에는 미흡하였다. 이에 따라 우리나라 옛 사람 시료 연구에 MLPA 기법을 적용할 때 현재 임상적으로 사용되는 기법의 단순 적용만으로는 성공하기 어려우며 이를 보완한 추가적 기술 개발이 앞으로 필요한 상태임을 알게 되었다. Multiplex Ligation dependent Probe Amplification (MLPA) to detect large deletions or duplications has been widely used as a diagnostic tool for various disease clinically. As this method requires only a small amount of template DNA and is very simple and high throughput, it has numerous advantages for the analysis of the human specimen obtained from archaeological sites. In this study we therefore tried to perform MLPA analysis for detecting any of duplications or deletions in mummy samples (n=4) from medieval Joseon tombs of Korea. Of them, we could not get any authentic data from 3 samples by MLPA method while only one case (HD2) showed the possible presence of duplications or deletions during her lifetime. Although the current report reveal that MLPA is a promising tool for anthropological study in South Korea, more studies are still needed to make up for the validity problem of commercial MLPA kit used in this study.

생물학 ; 고품질의 Rotifer와 Artemia의 생산을 위한 해양세균 이용과 대량생산에 따른 환경인자에 관한 연구 1 . Erythrobacter sp . S π - Ⅰ에 의한 Rotifer , Brachionus plicatilis의 배양시 지방산과 아미노산 조성의 변화

이원재(Won Jae Lee),박유수(You Soo Park),박영태(Young Tae Park),김성재(Sung Jae Kim),김광양(Kwang Yang Kim) 부경대학교 기초과학연구소 1998 부경대 기초과학연구논문집 Vol.1 No.-

레크리에이션의 사회심리학적 연구동향

정명수(Chung Myoung Soo),김유수(Kim You Soo) 조선대학교 교과교육연구소 1999 교과교육연구 Vol.20 No.1

Most studies in Korea and Japan on Recreation have used the social survey as a research method and some studies have used speculative methods which are used to attempt principal investigation. However, studies in western countries, including America and Canada have focused on many fields, even including sociology, psychology, economics, industrial engineering and ecology. Socioloical studies on Recreation have been an essential area, since publishing of “the theory of leisure class : an economic study in the evolution of institutions” of Vebren, T.B.(1899). Leisure sociology, based on practical data appeared in 1920 to 1930. After publishing of mass leisure of Larrbee, E and Meyersohn, R.B., the mass leisure era appeared in 1950 to 1960 and researchers took a growing interest in much broader fields. From 1960 to the present, the number of studies on recreation have increased and extended to many fields. Since 1960, researchers on recreation have gazed with deep interest and anxiety on practical studies to apply its’ results to everyday life. Although it was Kaplan who gave a definition of recreation from objective and subjective standpoints, Many studies have attempted to analyze one’s mental state, experience and attitude, which are existent in the subject of behavior by psychological concept from subjective standpoint. Many psychological studies on recreation published from the late 1960’s to the early 1970’s. During that time, the psychological studies focused on amusements, motive, personality, socialization and metal hygienic effects of recreation. And also, experimental studies and analytic studies by case studies were published.

고품질의 Rotifer 와 Artemia 의 생산을 위한 해양세균 이용과 대량생산에 따른 환경인자에 관한 연구 1 . Erythrobacter sp. S π-1 에 의한 Rotifer , Brachionus plicatilis 의 배양시 지방산과 아미노산 조성의 변화

이원재(Won Jae Lee),박유수(You Soo Park),박영태(Young Tae Park),김성재(Sung Jae Kim),김광양(Kwang Yang Kim) 한국수산과학회 1997 한국수산과학회지 Vol.30 No.3

To develop a beneficial microbial feed for the cultivation of rotifer, Brachionus plicatilis, an aerobic photosynthetic bacterium, Erythrobacter sp. S π-Ⅰ was isolated from marine structure at Haeundae beach in Pusan, Korea. Feeding effects of Frythrobacter sp. S π-Ⅰ on the growth of rotifer were analyzed comparing to other feeds such as PSB (purple nonsulfur bacteria), Chlorella sp. and baker`s yeast. Erythrobacter sp. S π-Ⅰ contained more linoleic acid (C_(18:3ω3)) and oleic acid (C_(18:1ω9)) and amino acids than PSB (purple nonsulfur bacteria), Chlorella sp. and baker`s yeast. The rotifer fed on Erythrobacter sp. S π-Ⅰ showed better effects than those fed on other feeds in the individual growth, size and weight. Also, the rotifer especially contained more eicosapentaenoic acid (C_(20:5ω3)) and docosahexaenoic acid (C_(22:6ω3)) in case of Erythrobacter sp. S π-Ⅰ feeding than the other feeds. In case of the feed of PSB and baker`s yeast, docosahexaenoic acid (C_(22:6ω3)) did not show. In amino acid analysis, the rotifer fed on Erythrobacter sp. S π-Ⅰ showed more amino acid content comparing to those fed on other diets. Especially, arginine, isoleucine, histidine, lysine, methionine, phenylalanine, threonine, which are essential amino acid for fish growth, showed high contents. These results suggested that the aerobic photosynthetic bacterium, Erythrobacter sp. S π-Ⅰ would be a beneficial microbial feed for the cultivation of rotifer.

전방십자인대 재건술 후 가속 재활 효과의 평가를 위한 새로운 등속성 지표

이인식 ( In Sik Lee ),임재영 ( Jae Young Lim ),김유수 ( You Soo Kim ),정선근 ( Sun Gun Chung ),한태륜 ( Tai Ryoon Han ),김태균 ( Tae Kyun Kim ) 대한스포츠의학회 2005 대한스포츠의학회지 Vol.23 No.3

레크리에이션의 사회심리학적 연구동향

정명수,김유수 조선대학교 사범대학 부설 교과교육연구소 1999 敎科敎育硏究 Vol.2 No.1

Most studies in Korea and Japan on Recreation have used the social survey as a research method and some studies have used apeculative methods which are used to attempt principal investigation. However, studies in western countries, including America and Canada have focused on many fields, even including sociology, psychology, economics, industrial engineering and ecology. Socioloical studies on Recreation have been an essential area, since publishing of "the theory of leisure class: an economic study in the evolution of institutions" of Vebren, T.B.(1899). Leisure sociology, based on practical data appeared in 1920 to 1930. After publishing of mass leisure of Larrbee, E and Meyersohn, R.B., the mass leisure era appeared in 1950 to 1960 and researshers took a growing interest in much broader fields. FRom 1960 to the present, the number of studies on recreation have increased and extended to many fields. Since 1960, researchers on recreation have gazed with deep interest and anxiety on practical studies to apply its' results to everyday life. Although it was Kaplan who gave a definition of recreation from objective and subjective standpoints, Many studies have attempted to analyze one's mental state, experience and arritude, which are existent in the subject of behavior by psychological concept from subjective standpoint. Many psychological studies on recreation published from the late 1960's to the early 1970's. During that time, the psychological studies focused on amusements, motive, personality, socialization and metal hygienic effects of recreation. And also, experimental studies and analytic studies by case studies were published.

사회심리적요인에 따른 스포츠소비행동 연구

정명수,박준희,김유수 조선대학교 통계연구소 1999 統計硏究所論文誌 Vol.1 No.1

The purpose of this study was to identify the social and psychological variables associated with sport consumption. A questionnaire consisted of 101 items measuring the social and psychological factors related to sport consumption and basic items asking the subject's demographic characteristics and amount of sport consumption through various means was administered to 414 Collegians(159 male and 255 female). The means of spot consumption investigated were viewing sport events in actual sport situations, viewing sports through T.V.. listening to sport broadcasting through radio. and reading newspapers, magazines and books on sports. Factor analyses were conducted on the 101 items and 17 social and psychological factors were found. The social and psychological factors were then used as independent variables for multiple regression analyses in order to identify the variables associated with various means of sport consumption. The main results indicated the following conclusions : (1) The social and psychological variables related to the general sport consumption were learning of sport skills and strategies, entertainment, socialization, curiousty, aesthetics, avoidance of reality, aggression, locality catharsis, stress-seeking, dependance and identification. (2) Variables such as socialization, learning of sport skills and strategies and entertainment were the common variables for all sport consumption besides the common variables. (3) Different social and psychological variables were identified for different means of sport consumption besides the common variables.