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손형식(Hyeng-Sik Son),손희종(Hee-Jong Son),김미아(Mia Kim),이상준(Sang-Joon Lee) 한국생물공학회 2011 KSBB Journal Vol.26 No.5
Microbial fuel cell (MFC) wes enriched using sludge in wastewater treatment. The microbial community of activated sludge and enriched MFC were analyzed by FISH (fluorescent in situ hybridization) and 16S rDNA sequencing. Bacteroidetes group were pre-dominant in activated sludge by FISH. α group, γ group and Acintobacter group were dominant and they were similar to distribution. The average value of 10 peak of MFC is 0.44C. When MFC wase enriched by sludge, γ-Proteobacteria, Plantomycetes group increased 70% and 60%, respectively. In results of 16S rDNA sequencing, Sphiringomonas sp. was comprised in α proteobacteria and Enterobacter sp., Klebsiella sp., Acinetobacter sp., Bacillus sp. were comprised in γ proteobacteria and Chryseobacterium sp. was comprised in Flavobacteria were isolated from sludge.
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손형식(Hyeng Sik Son),손희종(Hee Jong Son),김미아(Mia Kim),이상준(Sang Joon Lee) 大韓環境工學會 2010 대한환경공학회지 Vol.32 No.12
낙동강, 회동 및 기장에서 채집한 sediment의 미생물 군집을 FISH 분석을 통하여 조사한 결과, α 그룹, Acidobacter 그룹 및 Cyanobacter 그룹의 분포비율이 가장 높았으며 전체적으로 서로 유사한 분포 특성을 나타내었다. 각각의 sediment를 접종한 MFC 농화배양 이후의 coulombic yield는 낙동강, 회동 및 기장의 경우 각각 0.64 C, 0.50 C, 0.61 C로 나타났으며, 농화배양 완료 후의 미생물 군집분포는 β-Proteobacteria, γ-Proteobacteria, Acidobacter 그룹 및 Firmicutes 그룹이 농화배양 전보다 각각 45~90%, 50~90%, 40~80% 및 45~125% 정도 생체량이 증가한 것으로 나타났다. 농화배양이 끝난 후 16S rDNA를 이용한 미생물 동정결과에서, 낙동강 sediment를 주입한 MFC의 경우는 α-Proteobacteria의 속하는 Roseomonas sp., Azospillum sp.와 γ-Proteobacteria의 Frateuria sp., Dyella sp., Enterobacter sp.와 Deinococci 그룹의 Deinococcus sp.가 동정되었고, 기장 sediment는 α-Proteobacteria의 Azospillum sp.와 β-Proteobacteria의 Delftia sp., Ralstonia sp.와 γ-Proteobacteria의 Klebsiella sp.와 Deinococci 그룹의 Deinococcus sp.가 동정되었으며, 회동 sediment는 γ-Proteobacteria의 Pseudomonas sp., Klebsiella sp.와 Deinococci 그룹의 Deinococci sp.와 Actinobacteria 그룹의 Leifsonia sp.와 Bacilli 그룹의 Bacillus sp.가 동정되었다. Microbial fuel cells (MFC) were enriched using sediment Nakdong river, Hoidong river and protected water area in Gijang. The microbial community of sediment and enriched MFC was analyzed by FISH (fluorescent in situ hybridization) and 16S rDNA sequencing. α-Proteobacteria, Acidobacter and Cyanobactia group were dominant in sediment by FISH. The coulombs of the final 10 peak of the 3 MFC (Nakdong, Hoidong, Gijang) were 0.64 C, 0.50 C, 0.61 C, respectively. When MFCs were enriched by sediment, β-, γ-Proteobacteria, Acidobacter and Firmicutes group increased 45~90%, 50~90%, 40~80% and 45~125%, respectively. In results of 16S rDNA sequencing, Roseomonas sp., Azospillium sp., Frateuria sp., Dyella sp., Enterobacter sp. and Deinocossus were isolated from Nakdong river and Azospillium sp., Delftia sp., Ralstonia sp., Klebsiella sp. and Deinococcus sp. were isolated from protected water area in Gijang and Pseudomonas sp., Klebsiella sp., Deinococcus sp., Leifsonia sp. and Bacillus sp. were isolated from Hoidong river.
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동해안 4개 석호의 수질과 식물플랑크톤 계절별 변화에 관한 연구
문병렬 ( Byeong Ryeol Moon ),전현진,전숙례 ( Sook Lye Jeon ),이재선 ( Jae Sun Lee ),신지은 ( Ji Eun Shin ),안지혜 ( Ji Hye Ahn ),양용운 ( Yong Woon Yang ),현문식 ( Moon Sik Hyun ),김미아 ( Mia Kim ) 한국환경과학회 2015 한국환경과학회지 Vol.24 No.9
The seasonal variation of phytoplankton communities and its standing crops in accordance with environmental factors were investigated at Yeongrang-ho, Cheongcho-ho, Mae-ho and Hyang-ho which are located in the East coast of Korea during April to October 2011. High concentrations of T-N and T-P were observed in four lagoons due to the inflowing nutrients from farmlands and residential areas near lagoons. Nutrient salts mainly composed of nitrogen and phosphate caused the eutrophication of the lakes, leading to the robust growth of phytoplankton. During the period of investigation, 64 to 107 taxa of phytoplankton were observed in four lagoons. Diatoms were the most dominant species with high density. Non-point pollution source was estimated as the main cause of water pollution of lagoons. Trophic states of lagoons evaluated using the value of TSI and TSIKO indicated that all the investigated lagoons were in the eutrophication state.
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