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Enzyme-linked immunosorbent assay를 이용한 바이러스성 출혈성 패혈증 바이러스 감염 넙치(Paralichthys olivaceus)의 특이 항체반응 검사
황지연,장진현,김동준,권문경,서정수,황성돈,손맹현,Hwang, Jee Youn,Jang, Jin Hyeon,Kim, Dong Jun,Kwon, Mun Gyeong,Seo, Jung Soo,Hwang, Seong Don,Son, Maeng-Hyun 한국수산과학회 2017 한국수산과학회지 Vol.50 No.5
The viral hemorrhagic septicemia virus (VHSV) has an extensive host range, and infects farmed and wild fish inhabiting both freshwater and marine ecosystems. Enzyme-linked immunosorbent assay (ELISA) is highly useful in diagnosing viral hemorrhagic septicemia. However, ELISA shows high, non-specific background reaction with fish antibodies. In this study, we optimized the antigen and antibody concentrations used for detecting specific antibodies in VHSV-infected olive flounder to reduce non-specific binding, and improve the sensitivity of ELISA. The results suggested that OD (optical Density) values were valid when ELISA was performed with $0.1{\mu}g/well$ of virus, involving blocking with blocking buffer (Roth, Roti-Block), 1:300-1:600 dilution with flounder antisera, and 1:1000 dilution with anti-flounder IgM and HRP-conjugated goat anti-mouse IgG for detecting the VHSV antibody in flounder sera. Furthermore, 11 different VHSV strains isolated in Korea from 2012 to 2016 were used to infect the fish. The results showed no correlation between viral pathogenicity and antibody production. This research is a basic study on the application of antibody detection in the diagnosis of viral hemorrhagic septicemia in the olive flounder.
김주훈 ( Ju Hun Kim ),주성웅 ( Seong Woong Joo ),김동준 ( Dong Jun Kim ),김수영 ( Soo Young Kim ),황진일 ( Jin Il Hwang ),이형철 ( Hyung Chul Lee ),유병돈 ( Byung Don You ) 대한금속재료학회(구 대한금속학회) 2014 대한금속·재료학회지 Vol.52 No.10
The reduction behavior of molten electric arc furnace (EAF) slag containing (FeO), (MnO) and (P2O5) was investigated using graphite as a reductant. Reaction temperature (1400-1600 ℃) and slag basicity (0.5-2.0) were varied during the experiment. Alumina and magnesia crucibles were used to observe the effect of refractory on the reduction behavior of the molten EAF slag. When an alumina crucible was used, (Al2O3) content in the slag increased significantly by its elution from the crucible. The content of poorly reducible oxides, (CaO), (MgO), and (SiO2), maintained an almost constant level regardless of the reaction temperature, because the increase of (Al2O3) and the decrease in reducible oxides (FeO, MnO, P2O5 etc.) offset one another. The reduction degree of reducible oxides evaluated through chemical composition analysis agreed well with that calculated by off-gas analysis. The reduction degree increased with an increase in the reaction temperature and the slag basicity. The reduction degree in the magnesia crucible was higher than that in the alumina crucible at identical conditions. When a magnesia crucible was used, the reduction reaction was more promoted due to the enhancement of the activity of (FeO) and (MnO), compared to the alumina crucible.