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RISS 인기검색어
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- 저자 : 권일찬(Yil Chan Kwon) (검색결과 4 건)
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정찬식(Chan Sik Chung),권일찬(Yil Chan Kwon),김병주(Byung Joo Kim),한원영(Won Young Han),곽용호(Yong Ho Kwack),임무상(Moo Sang Lim),손창기(Chang Ki Son),이야성(Ya Seong Lee) 한국육종학회 2002 한국육종학회지 Vol.34 No.1
A new pea variety, Olwandu , was developed at the National Yeongnam Agricultural Experiment Station (NYAES) in 1998. Olwandu was selected from a cross Sparkle/Wensume in 1987. The advanced and regional yield trials were carried out from 1994 to 1995 and
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Sang Ik Han(韓尙益),Hyun Tae Kim(金賢泰),Yil Chan Kwon(權一瓚),Chang Kie Park(朴昶璂),Yong Ho Kwack(郭龍鎬),Ill Kyung Chung(鄭鎰炅) 한국육종학회 1997 한국육종학회지 Vol.29 No.1
This study was conducted to determine the pattern of self-incompatibility through self-and cross-pollination in two varieties of herbaceous peony (Paeonia lactiflora Pallas). After self-pollination with the pollens of same variety, pollen tubes have germinated and grown into style tissue. However, the pollen tubes have arrested the growth in the mid of growing stage. This pattern was a different from that of sporophytic self-incompatibility. In the case of sporophytic self-incompatibility, self-pollinated pollens are not germinated on the style in normal, but if the pollens do, they do not penetrate into style tissue. From these phenomena, the pattern of self-incompatibility in herbaceous peony is assumed gametophytic self-incompatibility. For more precise research, protein analysis using SDS-PAGE and RNase activity stain gel electrophoresis was conducted. In SDS-PAGE analysis, two major proteins (21.5 and 28-kDa) was detected, and ribonuclease activity was tested in activity stain gel. Ribonucleatic activity is a unique characteristic of stylar proteins of gametophytic self-incompatibility plants reported previously. From these results, we concluded that P. lactiflora has a gametophytic self-incompatibility.
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완두(Pisum sativum L.) 葉肉細胞 原形質體의 分雜 및 融合
權一瓚,金仁燮,金達雄 慶北大學校 農業科學技術硏究所 1988 慶北大農學誌 Vol.6 No.-
완두葉肉細胞의 原形質體分離 및 融合에 있어서 酵素의 處理時間, CaCl₂·2H₂O의 效果, 酸素의 濃度와 葉의 位置에 따른 原形質體의 分離에 미치는 影響과 Mole 濃度에 따른 遠心分離效果, PEG溶液의 處理時間, 酸度에 따른 融合效果 그리고 dimethyl sulfoxide 添加效果를 究明하기 위하여 實施한 實驗의 結果는 다음과 같다. 1. 原形質體의 分離에는 4時間의 酸素處理가 가장 良好했고 CaCl₂·2H₂O의 添加에 따라 原形質體의 分離速度가 늦어졌으며 活性度는 4時間까지 變動이 없었고 CaCl₂·2H₂O에 의해서 滑性度가 長期間 維持되었다. 2. 酸素의 濃度는 1%이상의 處理에서 거의 同一한 原形質體分離率을 나타냈으며 上位 1∼2葉이 가장 좋은 原形質體分離率을 보였으며 遠心分離效果는 0.4M, 0.5M에서 良好하였다. 3. 原形質體融合은 PEG 6000溶液 75分 處理에서 좋은 融合率을 보였다. 그러나 30分 以上의 處理가 活性度에 影響을 미치지 않았으며 pH 5.8인 PEG 6000의 0.2M溶液에서 가장 높은 62.84%의 融合率을 나타내었다. dimethyl sulfoxide에 의한 效果는 融合率이 3∼7% 減少하지만 binucleate의 誘道에 有效한 것으로 推定된다. This experiment was conducted to identify the enzyme treatment time, calcium ion effect, enzyme concentration and leaf position for protoplast isolation. It was also performed to determine the adequate molarity on protoplasts, and to investigate the incubation time, pH, PEG concentration and DMSO effect for protoplast fusion. The results obtained were summarized as follows; The optimal time of incubation in enzyme solution was 4 hours. And the protoplast releasing time was delayed by CaCl₂·2H₂O addition to the enzyme solution compared with no added one. The viability had kept up to above 95% until the 4 hours after digestion. The high viability of the protoplast was preserved more than 16 hours by adding CaCl₂·2H₂O to digestion solution. The enzyme concentration had no effect on protoplast yield in range from 1% to 5% and the first or second leaf from the top of the plant produced the highest protoplast yield among the leaf position tested. The purity of healthy protoplast was better in 0.4M and 0.5M sucrose than in others, and the percentage of protoplast aggregation was more 20% to 50% in PEG 6,000 compared with 4,000 and PEG 1,500. Even though the percentage of protoplast aggregation was less increased by 3% to 7% than without DMSO, its treatment was effective to induce binucleated protoplasts.
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