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권명상,김영후,조정순,Kwon Myung-Sang,Kim Young-Hwu,Cho Jung-Soon 동아시아식생활학회 2005 동아시아식생활학회지 Vol.15 No.5
In recent years, propolis has attracted much attention as an useful substance in medicine and functional food, even if it is known as a natural remedy in folk medicine since ancient times. propolis was registered as natural food since 1995 on Korean Food Act by Korean Food and Drug Administration(KFDA). The present study demonstrated the optimization of isolation of crude propolis by ethanol, and tumoricidal effect of pro polis. The optimal concentration of ethanol to separate a high quantity of propolis was $60\%$. The cytotoxic effect of ethanol extracted propolis against various cancer cell lines including murine lymphoma (Sarcoma-180), murine T-lymphoma (YAC-1), human breast carcinoma (MCF-7), human gastric carcinoma (KATOIII) and human hepatocellular carcinoma (Hep3B) and human lung adenocarcinoma (A-549) was observed using SRB and MIT assay. In order to investigate the curative activity by oral administration of propolis on tumor, ICR mice was subcutaneously implanted Sarcoma 180. In 300mg/kg and 600mg/kg propolis administered group, development of implanted tumors was inhibited by $40.9\%\;and\;67.9\%$ at 16th day, respectively. In the same dose of propolis administered group, development of implanted tumors was inhibited more strongly with dose dependent manner. Therefore, these data suggested propolis may show tumoricidal effects. In conclusion, these results indicate that propolis, one of the few natural remedies, can be used as functional food with tumoricidal effects.
權明相 건국대학교 1981 論文集 Vol.13 No.1
Mycological survey was carried out on total 475 samples including raw milk(62), market milk (93) and milk infected with mastitis(320). All of the samples were collected in four seasons. The results obtained were summarized as follows; 1.The total occurrence of fungi per sample were 75.8% in raw milk, 20.4% in market milk and 15.9% in milk infected with mastitis. 2.The kind of fungi that deteted in this experiment were Phycomycetes, Ascomycetes and Fungi Imperfecti. Genera frequently isolated in all of the samples were Penicillium app. (20.1%), Aspergillus spp. (15.0%) and Moni1ia sap. (12.8%). 3.On the seasonal variations of fungal distribution, Ascomycetes frequently isolated in cold season but Fungi Imperfecti isolated in warm season. Especially, Penicillium sppy., Aspergillus app. and Candida app. were frequently during the cold season, whereas Mucor spp., Fusarium spp. and Geotrichum spp. were more frequent in summer and autumn.