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      • KCI등재

        시판김치로부터 젖산균의 분리 및 동정

        고정림(Jung-Lim Ko),오창경(Chang-Kyung Oh),오명철(Myung-Cheol Oh),김수현(Soo-Hyun Kim) 한국식품영양과학회 2009 한국식품영양과학회지 Vol.38 No.6

        본 연구에서는 시판 김치로부터 분리된 젖산균을 동정하였다. 제조 후 4일 동안 실온에서 배양된 배추김치에서 12주, 2일 동안 실온에서 배양한 총각김치에서 6주, 5일 동안 실온에서 배양한 깍두기에서 15주, 그리고 4일 동안 배양한 파김치에서 23주의 젖산균이 분리되었다. 배추김치에서 분리된 12주의 젖산균 중 8주는 Lactobacillus plantarum로, 1주는 Leuconostoc lactis로, 2주는 Lactobacillus casei subsp. pseudoplantarum으로, 그리고 1주는 Lactobacillus sake로 동정되었다. 총각김치에서 분리된 6주의 젖산균 중 3주는 Leuconostoc paramesenteroides로, 2주는 Leuconostoc mesenteroides subsp. mesenteroides로, 그리고 1주는 Lactobacillus plantarum로 동정되었다. 깍두기에서 분리된 15주 중 2주는 Leuconostoc lactis로, 3주는 Leuconostoc mesenteroides subsp. dextricum로, 4주는 Lactobacillus casei subsp. pseudoplantarum로, 그리고 4주는 Lactobacillus coryniformis subsp. torquens로 동정되었다. 파김치에서 분리된 23주 중 22주는 L. plantarum으로 그리고 1주는 Lactobacillus sake로 동정되었다. 이상의 결과로부터, 배추김치에서는 L. plantarum이, 총각김치에서는 L. paramesenteroides이, 깍두기에서는 L. casei subsp. pseudoplantarum과 L. coryniformis subsp. torquens이, 그리고 파김치에서는 L. plantarum이 가장 많이 동정되었다. 특히 파김치에서는 선발된 23주 중 1주를 제외하고는 모두 L. plantarum으로 동정되었다. This study was carried out to identify lactic acid bacteria isolated from commercial Kimchi. Twelve lactic acid bacteria strains were isolated from Chinese cabbage kimchi (Baechu kimchi) that was fermented for 4 days at room temperature after making kimchi, 6 strains from pickled ponytail radishes (Chongkak kimchi) that was fermented for 2 days, and 15 strains in radish cube kimchi (Kaktugi) that was fermented for 5 days, and 23 strains were isolated in pickled Wakegi (Pa kimchi) that was fermented for 4 days. Eight strains among the lactic acid bacteria of 12 strains isolated from Baechu kimchi (pH 4.0) were identified as Lactobacillus plantarum, 1 strain as Leuconostoc lactis, 2 strains as Lactobacillus casei subsp. pseudoplantarum, and 1 strain as Lactobacillus sake. Three strains among the lactic acid bacteria of 6 strains isolated from Chongkak kimchi (pH 4.5) were identified as Leuconostoc paramesenteroides, 2 strains as Leuconostoc mesenteroides subsp. mesenteroides, and 1 strain as Lactobacillus plantarum. Two strains among the 15 strains isolated in Kaktugi (pH 4.0) were identified as Leuconostoc lactis, 3 strains as Leuconostoc mesenteroides subsp dextranicum, 4 strains as Lactobacillus casei subsp. pseudoplantarum, and 4 strains as Lactobacillus coryniformis subsp. torquens. Twenty-two strains among the 23 strains isolated from Pa kimchi (pH 4.1) identified as L. plantarum and 1 strain was as Lactobacillus sake. From the results above, the dominant species of Baechu kimchi was confirmed as L. plantarum, Chongkak kimchi as L. paramesenteroides, Kaktugi as L. casei subsp. pseudoplantarum and L. coryniformis subsp. torquens, and Pa kimchi as L. plantarum.

      • KCI등재

        시판 김치 분리 젖산균에 의한 아질산염 소거

        고정림(Jung-Lim Ko),오창경(Chang-Kyung Oh),오명철(Myung-Cheol Oh),김수현(Soo-Hyun Kim) 한국식품영양과학회 2009 한국식품영양과학회지 Vol.38 No.7

        본 연구는 시판 배추김치, 총각김치, 깍두기 및 파김치에서 분리한 젖산균을 15℃와 25℃의 온도에서 배양하였을 때아질산염의 소거 효능을 확인하였다. 15℃에서, 시판김치에서 분리된 젖산균은 L. mesenteroides subsp mesenteroides와 L. paramesenteroides를 제외하고는 매우 활성적으로 아질산염을 소거시켰다. 특히 L. mesenteroides subsp dextrinicum과 L. lactis는 아질산염에 적응하는 기간을 필요로 함이 없이 매우 활성적으로 아질산염을 소거시켰다. L. sake, L. plantarum, L. casei subsp. pseudoplantarum 및 L. coryniformis subsp. torquens는 하루 정도 아질산염에 적응하는 기간을 거친 이후에 매우 활성적으로 아질산염을 소거시켰다. L mesenteroides subsp. mesenteroides는 하루가 경과된 이후에 비교적 활성적으로 아질산염을 소거시켰다. 그러나 L. mesenteroides subsp. mesenteroides는 다른 균종에 비하여 매우 낮은 아질산염 소거율을 나타내었다. 25℃에서, 시판김치에서 분리된 모든 젖산균은 아질산염에 적응하는 기간을 필요로 하지 않았으며, L. paramesenteroides를 제외하고는 매우 활성적으로 아질산염을 소거하였다. 또한 시판김치로부터 분리된 젖산균은 L. mesenteroides subsp. mesenteroides와 L. paramesenteroides를 제외하고는 1일 이후에, L. mesenteroides subsp. mesenteroides는 2일 이후에 90% 이상의 아질산염을 소거시켰다. 그러나 L. paramesenteroides는 25℃에서도 활성적이지 못하여 다른 균종에 비하여 아질산염의 소거 능력이 매우 낮았다. 한편, 다른 김치 기원의 동일 균종의 Lactobacilli와 L. mesenteroides subsp. mesenteroides 및 L. paramesenteroides를 제외한 Leuconostocs는 15℃에서는 물론 25℃에서 매우 활성적으로 아질산염을 소거시켰다. This study confirmed depletion efficiency of nitrite when incubate lactic acid bacteria was isolated from commercial Chinese cabbage pickles (Baechu kimchi), pickled ponytail radishes (Chongkak kimchi), radish cube kimchi (Kaktugi) and pickled Wakegi (Pa kimchi) at temperatures of 15℃ and 25℃. At 15℃, lactic acid bacteria isolated from commercial kimchi depleted nitrite actively except Leuconostoc mesenteroides subsp. mesenteroides and Leuconostoc paramesenteroides. In particular, Leuconostoc mesenteroides subsp. dextrinicum and Leuconostoc lactis depleted nitrite by very actively without period adaptation in nitrite. Lactobacillus sake, Lactobacillus plantarum, Lactobacillus casei subsp. pseudoplantarum, and Lactobacillus coryniformis subsp. torquens depleted nitrite very actively after 1 day. L. mesenteroides subsp. mesenteroides depleted nitrite relatively actively after 2 days pass. In contrast, L. paramesenteroides displayed very low nitrite depletion ratio compared to other species. At 25℃, all lactic acid bacteria isolated from commercial kimchi did not need adapting period in nitrite, and depleted nitrite very actively except L. paramesenteroides. Also, all lactic acid bacteria except L. mesenteroides subsp. mesenteroides and L. paramesenteroides nitrite of more than 90% after 1 day, and L. mesenteroides subsp. mesenteroides depleted nitrite of more than 90% after 2 days. However, because L. paramesenteroides was not active even at 25℃, nitrite depletion efficiency was very low compared to other species. On the other hand, the same species of Lactobacilli and Leuconostocs except L. mesenteroides subsp. mesenteroides and L. paramesenteroides of other kimchi origin at 15℃ as well as 25℃ by vitality depleted nitrite very actively without statistically significant difference (p<0.05).

      • KCI등재

        벨리댄스가 중년여성의 혈액성분, 골밀도, Osteocalcin에 미치는 영향

        고정림(Ko Jeong Rim),이현미(Lee Hyun Mi),전재영(Jeon Jae Young),백영호(Baek Yeong Ho) 한국생명과학회 2007 생명과학회지 Vol.17 No.12

        본 연구에서는 40대 중년여성 16명을 벨리댄스군 8명, 대조군 8명으로 구성하여 후 벨리댄스 그룹은 12주에 걸쳐 주3회 1회 60분 동안 운동을 지속적으로 실시하여 혈액성분과 골다공증, Osteocalcin을 분석한 결론은 다음과 같다. 1. WBC는 벨리댄스 그룹이 집단간, 집단내에서 유의한 감소를 나타내었다. 하지만 RBC, Hb, PLT은 모든 그룹이 집단내, 집단간 유의한 차이를 나타내지 못했다. 2. 골밀도는 벨리댄스 그룹이 집단간 유의하게 증가하였고, 집단내에서는 유의하게 증가한 것으로 나타났다. 3. Osteocalcin은 벨리댄스 그룹내 증가양상은 보였지만 유의성은 나타나지 않았다. 이상의 결과를 통해서, 벨리댄스가 WBC와 골밀도에 유익한 변화를 가져왔으며 이로 인하여 중년여성의 건강증진과 골다공증 및 골 관련 질환의 예방을 위해 큰 도움을 줄 것이며, 아직 국내에 벨리댄스에 대한 연구가 미흡한 실정이라 향후 장기간 연구가 필요할 것으로 사료된다. The purpose of this study was to develop a movement program adopted from Bellydance, and to analyze the effects of the Bellydance program on the blood Components and Deoxypyridinoline, Osteocalcin in middle aged women. The Bellydance program was composed of warming-up (10min), main exercise (40min) and cooling-down (10min) parts. The 40 middle-aged women between 40 and 60 year old were divided into two group; the control group (8 women), the Bellydance group (8 women). The Bellydance program was applied three times a 12weeks. The result of this study were as follow. RBC, Hb, WBC, PLT, Osteocalcin was measured in a whole blood. The bellydance group showed WBC was more significantly decreased than control group, but RBC, Hb, PLT were insignificant in other groups. The bellydance group showed Deoxypyridinoline was more significantly increased than control group, but Osteocalcin were insignificant in other groups. Therefore this program can be available in middle aged women.

      • 공사비 절감을 위한 공동주택 건축물에너지효율등급 인증 최적설계지침 가이드라인 연구

        고종철(Ko, Jong-Chul),이종인(Lee, Jong-In),김현배(Kim, Hyun-Bae),신지웅(Shin, Jee-Woong),고정림(Ko, Jung-Lim),안병욱(An, Byung-Wook),강승구(Kang, Seung-Ku) 한국건축친환경설비학회 2012 한국건축친환경설비학회 학술발표대회 논문집 Vol.2012 No.10

        In the present day, the international society is promoting policies to reduce greenhouse gas emission. In Korea, buildings consume over 25% of the total energy consumed. Contribution rates are applied in order to draw the utmost measures to reduce building energy consumption. In comparison to total construction cost, insulation is studied to yield the most contribution toward energy reduction. Heating method and glazing were the next. By applying the certification system, additional construction cost is about ₩700,000∼₩1,400,000 when upgrading level. Finally, the construction cost is going to add ₩30,000∼180,000 per 1% of energy saving. The insulation has highest efficiency among other materials used in the construction.

      • KCI등재후보

        기존건축물 에너지 성능 리모델링 사례분석을 통한 BIM 기반 프로세스의 활용에 관한 연구

        박경순(Park Kyung-Soon),고정림(Ko Jung-Lim) 한국건축친환경설비학회 2014 한국건축친환경설비학회 논문집 Vol.8 No.2

        Over the course of simulation performances, the existing buildings’ current situation of energy consumption is diagnosed, and the effect of improving energy performance is predicted in early planning stage. However, it is difficult to ensure the level of information required to perform the simulation. The main issues are the aging condition assessment, omission of required information of the building, and the differences between the documentations and the actual status of the existing building. BIM-based building information is able to digitalize and organize the documented building information and add changed or missing information in the field thereafter. In addition, a variety of tools and information increasing the compatibility are opening the potential uses for the remodeling. However, utilizing BIM for remodeling is not the norm, and the feedback-able actual operational problems information is insufficient. In this paper, the possibility of utilizing BIM-based remodeling is analyzed through a comparison of the existing and the BIM-based building energy analysis process of remodeling, and then the optimizing methods of remodeling process of the existing building is sought.

      • KCI등재

        조명 및 조명 제어 시스템의 그린 리트로핏 가이드라인 구축에 관한 연구

        구현오(Koo, Hyun Oh),고정림(Ko, Jeong-Lim),신지웅(Shin, Jee Woong),이건호(Lee, Keon ho) 한국건축친환경설비학회 2015 한국건축친환경설비학회 논문집 Vol.9 No.2

        Green remodeling in architectural field has been a major issue due to the expectations of amenity of spaces, energy and economical saving, and sustainability. The purpose of this paper is to provide the green retrofit guideline of lighting and control systems that encourage public to accumulate attention on greener and improved lighting systems in their spaces. In this study, lighting and control systems are examined through literature and case study investigation. The process of lighting and control systems remodeling was studied, and major issues and improvement directions were addressed. Moreover, the green lighting and control systems’ retrofit guideline with qualitative assessment of elements for office spaces was proposed.

      • KCI등재후보

        친환경 리모델링을 위한 BIM 기반 환경 및 에너지 성능분석 통합 프로세스 구축에 관한 연구

        박경순(Park Kyung-Soon),고정림(Ko Jung-Lim) 한국건축친환경설비학회 2013 한국건축친환경설비학회 논문집 Vol.7 No.2

        The aim of ensuring additional economics of building by reducing energy consumption and security of environmentally friendly performance of building are another important design objectives and criteria of remodeling. Accordingly, various certification systems and guidelines as a variety of design techniques have been proposed to ensure comfort of building occupants by improving environmental performance elements, such as light, heat, noise, airflow and etc. However, the development in quantitative evaluating and verifying techniques of environmental performance has not been provided as solidly as its stipulative background. BIM (Building Information Modeling), emerged as a new design technique, is able to ensure reliability and quantification of the proposed design through linking and utilizing BIM data for environmental performance analysis. The ability to analyze the environmental performance of BIM has been case studied, and they have emerged as big issues in the 2010s. In this study, BIM data utilization methodologies and applications in various areas for remodeling projects are sought. In addition, the environmental and energy performances analysis process that is useful in design practices for remodeling business is proposed.

      • KCI등재

        그린리모델링을 위한 BIM 기반 에너지 성능분석 입력변수 연계활용 프로세스에 관한 연구

        박경순(Park, Kyung-Soon),고정림(Ko, Jung-Lim) 한국건축친환경설비학회 2015 한국건축친환경설비학회 논문집 Vol.9 No.4

        Recently, in the field of construction, many researches to form 3D space information using BIM data and utilize BIM object information in architectural space into the process of implementing green building have been actively conducted largely by the industry-university-institute research agencies depending on the field of energy and environmental specialization. However, there are few quantitative evaluation criteria and specified methodologies in assessing environmental quality level depending on every field of light, sound, heat, and air current and also little performance evaluation in the field of energy for green remodelling of the existing architecture compared to the activated research topics. Accordingly, this study formed space information using BIM data and conducted a study on the measures to analyze and evaluate energy and environmental quality level of unit building more quantitatively and easily through shape and property information embedded in formed space information. Through this, this study analyzed BIM data linking process in the process of entering building shape information and organized BIM-based energy performance analysis input variable-utilizing process establishing plans for green remodelling. Through such a research process, this study aims to propose green remodelling energy performance analysis process improvement plans as evaluative methodology using BIM-based object information by linkable input variable items by field of energy quality level.

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