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이미애 ( Mi Aie Lee ),안성희 ( Sung Hee Ahn ),강윤숙 ( Yoon Suk Kang ),서문경애 ( Gyeong Ae Seomun ),신미자 ( Mi Ja Shin ) 한국의료윤리학회 2006 한국의료윤리학회지 Vol.9 No.2
A study was designed to evaluate the level of moral development of 197 nursing students from five nursing schools in Korea. Data was collected through a self-reported questionnaire completed between March and April 2006. The Korean version of the DIT (Rest`s Defining Issues Test) was used to evaluate the level of moral development as measured by the score of P and stage 4. The data was analyzed by a t-test, ANOVA. The results were as follows: 1) the mean score of P and stage 4 was 41.79 and 16.31 respectively; 2) the score of P did not reveal statistically significant differences with respect to demographic variables; 3) The score of stage 4 revealed significant differences related to the level of education of each participant`s mother. These results indicate that moral development is not influenced by demographic variables. We recommend that a database of DIT scores for students in nursing and other medical fields be created. Such a database would be helpful for the further development of nursing ethics curricula.
강윤숙,이숙정,김인자,안금희 대한간호행정학회 2008 간호행정학회지 Vol.14 No.3
Purpose: The purpose of this study was to develop a measurement scale of nursing competence for nurses working in general wards, and to test the validity and reliability of the scale. Method: A methodological study design was used, consisting of 3 steps as conceptual framework decision, measurement items development, and testing of validity and reliability. In order to verify content validity, 16 experts reviewed the concept of each competences and measurement items. The developed measurement items were tested from 301 nurses in one hospital. Factor analysis and item analysis were applied to establish the scale's construct validity and reliability. Result: The final scale consists of nine dimensions with 70 items. The overall scale reliability had a Cronbach's alpha of 0.98, and the demensions Cronbach's alpha ranged from 0.88 to 0.95. The factor loading of construct validity was from 0.40 to 0.83. The explained variance from the 9 extracted factors was 70.93% of the total variance, and final factors were labeled as critical thinking, resources management and professional development, ability of ward management, application of nursing process, ethical accountability, respectful consideration, patient centered approach, crisis management, and leadership. Conclusion: This measurement scale can be utilized to evaluate nursing competence for nurses working in general wards.
Chung, Yong Joon,Kong, In Soo,Kang, Yoon Suk,Yu, Ju Hyun 한국산업미생물학회 1990 한국미생물·생명공학회지 Vol.18 No.1
토양에서 분리한 호알카리성 Bacillus sp. YC-335가 생산하는 CGTase를 ethanol 침전법, DEAE-Toyopearl column chromatography, Sephadex G-100 column chromatography 방법 등에 의해 대량 정제하였다. 이 때 수율은 17.8%이었고 52.9배의 정제도를 가진 효소단백질을 얻을 수 있었다. 정제효소는 SDS-polyacrylamide gel 전기영동에 의해 분자량이 75,000 정도인 단일 peptide의 단백질임을 확인하였고 효소의 최적활성 pH는 6.0이었으며 pH 안정성은 pH 6-10까지 안정하였다. 최적활성온도는 50℃이었으며 열안정성은 50℃까지 안정하였고 이는 15mM CaCl_2에 의해 열안정성이 보호되었다. Alkalophilic Bacillus sp. YC-335 isolated from soil was capable of producing large amount of cyclodextrin glycosyltransferase(CGTase) in culture broth. This enzyme was successively purified 52.9 folds with 17.8 yield by ethanol precipitation, DEAE-Toyopearl column chromatography and Sephadex G-100 column chromatography. The purified enzyme have a molecular weight of approximately 75,000 estimated by SDS polyacrylamide gel electrophoresis. The optimum pH and temperature for the enzyme activity were 6.0 and 50℃, respectively. The enzyme was stable between pH 6 and 10, and up to 50℃. The thermostability of the enzyme was increased up to 60℃ by the addition of 15 mM CaCl_2.