정상인(正常人) 혈청(血淸)의 Propionibacterium acnes Serotype I 및 Serotype II에 대한 항체(抗體)

최철순,서용열,양용태,Choi, Chul-S.,Seo, Yang-Y.,Yang, Yong-T. 대한미생물학회 1979 大韓微生物學會誌 Vol.14 No.1

Antibodies to Propionibacterium acnes(Corynebacterium parvum) serotype I and serotype II in normal human sera were measured using a microtitre bacterial agglutination test. Of 168 sera tested, 53 sera(31.0%) exhibited higher agglutinin titres to serotype I than to serotype II and 34 sera(20.2%) gave higher titers to serotype II than to serotype I. Eighty-one sera(48.3%), however, showed similar antibody titres to both types. Antibodies to serotype I(x) and serotype II(y) showed high correlation(r=0.73, p<0.01) and regression equation was Y=1,078+0.73X. The mean antibody titre($log_2$) of 529 normal sera(male 447 and female 82) to serotype I was $5.49{\pm}1.36$, but there was no significant difference between male($5.45{\pm}1.36$) and female($5.74{\pm}1.36$). Bacterial agglutinin to Propionibacterium acnes in normal sera belonged to a 2-mercaptoethanol resistant IgG class.

正常人 血淸의 Propionibacterium acnes SerotypeⅠ 및 Serotype Ⅱ에 대한 抗體

崔哲淳(Chul S. Choi),徐容烈(Yong Y. Seo),梁容泰(Yong T. Yang) 大韓微生物學會 1979 大韓微生物學會誌 Vol.14 No.1

병원내 공기에서 분리된 응고효소-음성 포도구균의 균종과 항균제 감수성

박용호(Yong H Park),최철순(Chul S Choi),양용태(Yong T Yang) 大韓微生物學會 1988 大韓微生物學會誌 Vol.23 No.3

Yersinia enterocolitica O:1,2,3 군과 O:3 군의 균체응집항원과 침강항원의 특이성

김택선,최철순,양용태 중앙대학교 의과대학 의과학연구소 1989 中央醫大誌 Vol.14 No.1

By means of bacterial agglutination, agar gel-diffusion, rocket and two-dimentional immunoelectrophoresis, antigenic relationships of bacterial agglutinogens and precipitinogens between three strains of serogroup 0: 1,2,3 and two strains of serogroup 0:3 of Yersinia enterocolitica were studied. The results were summarized as follows: 1. Crossed bacterial agglutination reactions between strains of 0:1,2,3 and 0:3 to homologous and heterologous rabbit immune sera showed so close relations that strains of two serogroups were not differentiated one another by agglutination test using rabbit anti -0:1, -0:2 and -0:3 sera. 2. Bacterial antigens of 0:1, 2 ,3 cross-reacted with both anti-0:1 serum absorbed with 0:2 and 0:3 groups and anti -0:2 serum absorbed with 0:1 and 0:3 groups, whereas the bacterial antigens of 0:3 group reacted with only absorbed anti-0:1 serum. 3. Out of three bacterial soluble antigens extracted with 0.3% phenol-phosphate buffered saline (PBS), 0.1% Triton X-100-phosphate buffer and 10% trichloroacetic acid solution, the precipitinogen of PBS extract was most highly reactive and stable up to 121˚C for 30 min. The precipitinogen, however, heated at 60˚C for 30 min was more reactive. The heat-stable surface antigen of 0:1,2,3 group was antigenically identical with the antigen of 0:3 group. 4. Fused rocket immunoelectrophoresis revealed that the antigens of 0:1,2,3 and 0:3 groups had one to two common antigens which had a rapider mobility than group specific antigens. 5. By crossed immunoelectrophoresis, the precipitinogens of 0:1,2,3 group had four to five antigens having the relative electrophoretic mobility of 0, 0.15 - 0.18, 0.41, 0.47 -0.5 and 0.58, whereas the precipitinogens of 0:3 group had three antigens having the relative electrophoretic mobility of 0, 0.15 - 0.18 and 0.47 - 0.5. These results indicated that although the strains of 0:1,2,3, group would differ in their subagglutinogens from those of 0:3 group they belonged to 0:3 group by their common 0 group-specific precipitinogens.

Genus Bacillus의 병원성과 Superoxide Dismutase활성도와의 관계

김환수,정상인,양용태,최철순 중앙대학교 의과대학 의과학연구소 1991 中央醫大誌 Vol.16 No.2

This study was undertaken to examine the possibility of association between pathogenicity of Genus Bacillus and the induction of superoxide dismutase(SOD) activity. In this study, pathogenic B. anthracis ATCC 14578, vaccine strains; Army No.2 and Sterne, and other 4 strains of Bacillus species were included. The induction of SOD of Genus Bacillus was tested after cultivation by candle jar method. The results are summarized as follows: 1. Induction of total SOD activities was observed in pathogenic B. anthracis ATCC 14578 when cultured under reduced oxygen tension(candle jar method). (p>0.05) 2. Nonpathogenic strains, B. anthracis Army No. 2 and Sterne, and other 4 strains of related Bacillus species, were not observed the induction of SOD activity. 3. In the induction experiments, B. anthracis ATCC 14578 showed significant increment of Mn-SOD activity. But, other nonpathogenic strains failed to show statistically significant increase of Mn-SOD activity. These results suggest that the pathogenicity of Genus Bacillus may be associated with inducible activity of SOD.

Mycobacterium scrofulaceum에 의한 마우스 폐의 Superoxide Dismutase유도

이봉진,정상인,최철순,양용태 중앙대학교 간호과학연구소 1991 中央醫大誌 Vol.16 No.3

This study was undertaken to investigate whether the induction of superoxide dismutase in the lung was affected by infection with M. scrofulaceum. In addition, association between the plasmidbearing and induction of superoxide dismutase(SOD) were investigated with various strains of M. scrofulaceum. When M. scrofulaceum were injected to ICR-JCL mouse intravenously, type 42 CDC 1198 and type 43 Brooks strains induced superoxide dismutase in the lungs, which no significant changes in the activity of superoxide dismutase in the spleen were observed. Strains of M. scrofulaceum which possessed a large plasmid(>80 Kb)induced much oxygen metabolites in the host than the plasmidless strains. This result suggests that more virulent strains of M. scrofulaceum induces the activity of superoxide dismutase in the host to protect the injuries from the reactive oxygen species during the infection.

살무사(Agkistrodon blomhoffi brevicaudus)뱀독의 면역학적 성상

전우규,정상인,최철순,양용태 중앙대학교 의과대학 의과학연구소 1991 中央醫大誌 Vol.16 No.3

This study was undertaken to find out main toxic components in venom of Agkistrodon b. brevicaudus and to prepare antiserum to venom. Through the experiment, venom of A. b. brevicaudus was fractionated into 7 fractions through DEAE-Sephadex A-50 and observation were made with toxic reaction of fractioned venom of A. b. brevicaudus, neutralizing effect of antiserum for highly toxic fraction and finally, comparison of neutralizing effect of antisera for fractionated venom and original crude venom. The results ara summarized follows: 1. Fractionation of A. b. brevicaudus venom by DEAE sephadex A-50 column chromatograhpy resulted in seven peaks. The amount of total protein were the highest in 4th fraction. 2. To investigate the toxicity of each fraction, hemorrhagic activity of each fraction were compared. As a result, most strong hemorrhagic activity was shown in 6th fraction. Then 4th, 7th fraction were in that order. 3. Neutralizing effect of rat antisera for 2nd, 4th and 6th fraction were compared. Most effective neutralizing(antilethal) activity was shown in antiserum for 4th fraction and neutralizing effect of antiserum for 4th fraction was similar to that of crude venom.

Propionibacterium acnes Serotype Ⅰ 및 Ⅱ의 Sarcoma 180 복수암세포의 성장억제에 대한 비특이면역 효과

강영태,정상인,최철순,양용태 중앙대학교 의과대학 의과학연구소 1987 中央醫大誌 Vol.12 No.2

Although the anti-tumor effects of nonspecific active immunization with bacterial adjuvant producys may be limited, diverse biological response modifiers(BRM) including BCG, Corynebacterium parvum and Brucella abortus have been employed to enhance the immune response through the activation of macrophages and matural killer cells and to restore the T cell function of cancer patients. Propionibacterium acnes serotypeⅡ that is a predominant resident microorganism of the skin is most similar to previous descriotions of Corynebacterium parvum. In our previous study on the quantitative bacteriology of P. acnes isolated from the skin and serology with sera obtained from healthy persons, it was found that the distribution of serotypeⅠ was much higher than that of serotypeⅡ. However, the physiological role and significance of immunological function of two serotypes as BRM's were not elucidated. In this study, nonspecific tumor immunity of the saline extracts of cell wall components and ether extractedbacilli of P.acnes serotypeⅠand Ⅱ, and Brucella aboturs against the growth of Sarcoma 180 ascites tumor cells in ICR-JCR mice was assessed. The saline extracts of cell wall components appeared to be more effective than the ether extracted bacilli to inhibit the growth of ascites tumor cells. Mean survival days of mice administered with either the saline exyracts of cell wall components of P.acnes serotypeⅠor the ether extracted intact bacilli of P. acnes serotypeⅡ were significantly extended in comparison with that of control group. These results indicated that the enhancement of nonspecific immune response by P. acnes was associated with cell wall components, but there was no significant difference between two serotypes of P. acnes in antitumor immunization aganist Sarcoma 180 ascites cells in mice.

한탄바이러스 감염 제대정맥내피세포에 대한 단핵구 매개성 손상

정상인,김기우,문언수,강응택,유석희,최철순,양용태 중앙대학교 의과대학 의과학연구소 1992 中央醫大誌 Vol.17 No.4

Studies on the pathogenesis of hemorrhagic fever with syndrome(HFRS) in patients have been seriously hindered by the abscence of appropriate animal model. The major pathologic findings in patient with HFRS are generalized vasculopathy. However, the pathogenesis of disseminated intravascular coagulation (DIC) and bleeding tendency in patients with HFRS are not well understood. It has been reported that the specific viral antigens are usually demonstrated in the endothelium of glomerular capillaries, and resulted in application of primary cultured human umbilical vein endothelial of glomerular capillaries, and resulted in application of primary cultured human umbilical vein endothelial cells (HUVEC) to study on the pathogenesis of HFRS. In this study, HUVEC were infected with Hantaan virus. The outcome Hantaan virus infection of HUVEC were assessed by flurescence activated cell scanning(FACS). The result of FACS assay indicated that 11% of the cells contained veiral antigens on 3 days postinfection. The proportion of virus-containing cells increased to 85% by the fifth days, adn 96% by the seventh, respectively. These observed no CPE development in the HUVEC cells infected with Hantaan virus and observed up to 13 days. The sensitivity of immunohistochemical method used for the viral antigens appeared similar to that of indirect immunofluorescent antibody technique. The monocyte adherence rate of infected HUVEC was much higher that than that of uninfected HUVEC, The monocyte adherence rate of infected HUVEC was increased by addition of convalescent serum of patient with HFRS. Infection with Hantaan virus HUVEC stimulated the production of superoxide radical.