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약쑥(Artemisia princeps Pamp)의 항산화작용
홍정희,전정란,이주현,이인선,Hong, Jung-Hee,Jeon, Jeong-Lan,Lee, Ju-Hyun,Lee, In-Seon 한국식품영양과학회 2007 한국식품영양과학회지 Vol.36 No.6
본 연구는 약쑥(Artemisia princeps Pamp) 추출물의 항산화 효능을 조사하고자, 약쑥 분획물을 이용하여 총 폴리페놀 및 플라보노이드 함량, 활성산소종 소거능 및 간 microsome을 이용한 지질과산화 억제효과 등을 관찰하였다. 약쑥의 폴리페놀 함량 및 플라보노이드 함량은 EtOAc층에서 각각 $311.35{\mu}g/mg$ 및 $92.73{\mu}g/mg$으로 가장 높게 나타났다. DPPH radical 소거능은 EtOAc층의 $IC_{50}$이 $52.71{\mu}g/mg$으로 분획물 중에서 가장 높은 소거능을 보였다. Superoxideanion의 소거능은 EtOAc층의 $IC_{50}$은 $26.47{\mu}g/mg$으로, 합성항산화제인 BHA($IC_{50}:\;49.78{\mu}g/mg)$ 보다 항산화 효과가 우수한 것으로 나타났다. Hydrogen peroxide 소거능은 n-BuOH층의 $IC_{50}$이 $166.44{\mu}g/mg$으로, BHA의 소거활성보다 유의적(p<0.05)으로 높음을 알 수 있었다. Hydroxyl 라디칼 소거능도 EtOAc층의 $IC_{50}$값이 $58.92{\mu}g/mg$으로, BHA($IC_{50}:\;80.85{\mu}g/mg)$보다 유의적(p<0.05)으로 높았다. NO 소거능은 EtOAc층의 $IC_{50}$이 $65.94{\mu}g/mg$으로, BHA와 유의적인 차이가 없었다. 간 microsome에서의 지질과산화 억제효과도 EtOAc층이 분획물 중에서 가장 우수하였다. 이상의 결과로부터 약쑥 분획물의 EtOAc층이 총 폴리페놀 및 플라보노이드 함량이 높음을 알 수 있었으며, 이에 따른 활성산소종 소거능 및 간 microsome을 이용한 지질과산화 억제효과도 관찰할 수 있었다. Antioxidant properties of Artemisia princeps Pamp were determined using in vitro assay systems against 1,1-diphenyl-picrylhydrazyl (DPPH) radical, superoxide ($O_2{^-}$), hydrogen peroxide ($H_2O_2$), hydroxyl radical ($HO{\cdot}$) and nitric oxide (NO) radical, as well as rat liver microsomal lipid peroxidation. Among the five solvent fractions, ethyl acetate fractions showed the highest total polyphenol and flavonoid contents at $311.35{\mu}g/mg\;and\;92.73{\mu}g/mg$, respectively. Ethyl acetate fractions, except for $H_2O_2$ scavenging activity, also showed the highest scavenging activity; the 50% inhibitory concentration ($IC_{50},\;{\mu}g/mg$) values for DPPH, superoxide, $HO{\cdot}$ and NO radical scavenging were 52.71, 26.47, 58.92 and 65.94, respectively. Additionally, the highest inhibition of rat liver microsomal lipid peroxidation was observed by ethyl a cetate fraction.
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李海浜,金胄弼,全正蘭 동국대학교 산업기술대학원 1993 산업기술논문집 Vol.1 No.-
An arthropod community structure in a rice peddy field ecocystem was investigated. Sampling was conducted using D-vac sucker at the Experimental Station of Dongguk University in Namyangju-Gun, Kyonggi province during the periods from August to October in 1992. During the period of investigation, 112 species, 34 families and 6 orders were represented for the insect cummunity in untreated plots, while 104 species, 41 families and 5 orders in treated plots. And among them, 45 and 31 species of entomophagous insect were listed from the untreated and treated plots with 17.5% vs 5.2% in the occupancy rate, respectively, suggesting that pesticide usage lead the reduced number of immigrating and emigrating natural enemies. The other hand, 25 species in 13 families of the spider were represented in the untreated plots whereas 15 species in 8 families in treated plots. Spiders which collected in untreated plots were twice as high in abundance as those in treated plots. Tetragnatha sp. (Tetragnathidae) was the most abundance species in treated plots. Dominant species of untreated plots, Piratasubpiraticus (Lycosidae), occurred in very low number in treated plots. During the growing season, the temporal pattern of species diversity are measured using species diversity (H'), species richness (S) and species evenness index (J'). The tendency of communities to return to its predisturbed state were observed in treated plots. The positive correlation was obtained for spider species diversity and prey abundance in untreated plots but in treated plots.
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