HYDROGEN PRODUCTION FROM CHICKEN MANURE BY SUPERCRITICAL WATER GASIFICATION IN A FLUIDIZED BED REACTOR

Wen Cao,Changqing Cao,Liejin Guo,Hui Jin,Xiangdong Yao 한국신재생에너지학회 2015 AFORE Vol.2015 No.11

Cross-coupled fuzzy PID control combined with full decoupling compensation method for double cylinder servo control system

Jing Yao,Xiaoming Cao,Yang Zhang,Yao Li 대한기계학회 2018 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.32 No.5

The purpose of this thesis is to develop a double cylinder synchronization control system to address the high precision control requirement of this system. First, the dual-input dual-output coupling mathematical model of the hydraulic press synchronization control system was built using mechanism modelling. Then, the mathematical model of compensating and decoupling links is established by the input and load disturbance decoupling control method based on feedback compensation. Next, a cross-coupled fuzzy PID controller is designed, which is then combined with full decoupling compensation method. Finally, the coupling characteristics of the synchronization control system and the synchronization control performance of the hydraulic cylinders under the cross-coupled fuzzy PID controller combined with the decoupling control algorithm were tested and evaluated in a principle experiment platform. The results indicate that the robustness and the ability to resist interference load are improved under the theory and control strategy outlined in this paper.

Efficiently targeted therapy of glioblastoma xenograft via multifunctional biomimetic nanodrugs

Zhipeng Yao,Xiaochun Jiang,Hong Yao,Yafeng Wu,Fan Zhang,Cheng Wang,Chenxue Qi,Chenhui Zhao,Zeyu Wu,Min Qi,Jia Zhang,Xiaoxiang Cao,Zhichun Wang,Fei Wu,Chengyun Yao,Songqin Liu,Shizhang Ling,Hongping Xi 한국생체재료학회 2022 생체재료학회지 Vol.26 No.4

Background: Glioblastoma multiforme (GBM) is a fatal malignant primary brain tumor in adults. The therapeutic efficacy of chemotherapeutic drugs is limited due to the blood-brain barrier (BBB), poor drug targeting, and short biological half-lives. Multifunctional biomimetic nanodrugs have great potential to overcome these limitations of chemotherapeutic drugs. Methods: We synthesized and characterized a biomimetic nanodrug CMS/PEG-DOX-M. The CMS/PEG-DOX-M effectively and rapidly released DOX in U87 MG cells. Cell proliferation and apoptosis assays were examined by the MTT and TUNEL assays. The penetration of nanodrugs through the BBB and anti-tumor efficacy were investigated in the orthotopic glioblastoma xenograft models. Results: We showed that CMS/PEG-DOX-M inhibited cell proliferation of U87 MG cells and effectively induced cell apoptosis of U87 MG cells. Intracranial antitumor experiments showed that free DOX hardly penetrated the BBB, but CMS/PEG-DOX-M effectively reached the orthotopic ntracranial tumor through the BBB and significantly inhibited tumor growth. Immunofluorescence staining of orthotopic tumor tissue sections confirmed that nanodrugs promoted apoptosis of tumor cells. This study developed a multimodal nanodrug treatment system with the enhanced abilities of tumor-targeting, BBB penetration, and cancer-specific accumulation of chemotherapeutic drugs by combining chemotherapy and photothermal therapy. It can be used as a flexible and effective GBM treatment system and it may also be used for the treatment of other central nervous systems (CNS) tumors and extracranial tumors.

Doping effect on the adsorption of Na atom onto graphenes

Li-Hua Yao,Wen-Qiang Cao,Mao-Sheng Cao 한국물리학회 2016 Current Applied Physics Vol.16 No.5

We investigate the adsorption of Na atoms on pristine graphene and four types of defect graphenes using the first-principles calculations. The adsorption energies, electronic structures, Na storage capacities and the average potentials of Na atoms on different graphenes are calculated. The adsorption energies of Na atoms on B-doped, vacancy and B-doped vacancy graphenes are 1.93 eV, 2.46 eV, and 2.08 eV, respectively, which are much lower than that of Na on the pristine graphene (0.71 eV) and Na on Ndoped graphene (0.27 eV). The orbital hybridizations in the B-doped, vacancy and B-doped vacancy graphenes can be observed from the partial density of states, while there is no obvious orbital hybridization in the N-doped graphene as well as the pristine graphene. The B-doped vacancy graphene has the best Na storage capacity, while B-doped and vacancy graphenes also possess better Na storage capacities than those of the pristine graphene and N-doped graphene. This work demonstrates that the graphene introducing both B dopant and vacancy is expected to be a potential material for storing Na.

Research on the Dispersion Coefficient Measurement System based on CCD Imaging Automatic Alignment Technology

Cao Miao,Yao Qinghua 보안공학연구지원센터 2015 International Journal of Signal Processing, Image Vol.8 No.7

In order to measure the dispersion coefficient of glass and other transparent material rapidly and accurately, the paper studies the dispersion coefficient measurement system based on CCD imaging automatic alignment technology. On the basis of consideration of the air refractive index impacting on the measurement accuracy of dispersion coefficient, this paper rebuilds the mathematical model by the traditional dispersion coefficient measurement formula. Using the measuring angle technology of precision grating, the paper realizes the digital measurement of deflection angle of refracted ray. Sub-pixel edge detection algorithm is adopted to realize the automatic alignment of double line to single line, which has reduced artificial error of the dispersion coefficient in the measurement process. Finally, the paper combined the multichannel small collimator designed technology and the modern control technology to achieve the high precision measurement of refractive index. Demarcating the measurement system by known dispersion coefficient of optical glass, testing results and error of standard is less than ±2.3×10-6 absolutely.

Detection of the expression of a Bombyx mori Atypical Protein Kinase C in BmPLV-Infected Larval Midgut

Cao, Jian,He, Yuanqing,Li, Guohui,Chen, Keping,Kong, Jie,Wang, Fenghua,Shi, Jing,Yao, Qin Korean Society of Sericultural Science 2011 International Journal of Industrial Entomology Vol.22 No.2

Protein kinase C (PKC) is involved in many cellular signaling pathways, it participates in many physiological processes, such as cell cycle, growth, proliferation, differentiation and apoptosis. To investigate the effect of PKC on the silkworm midgut tissue infection of Bombyx mori parvo-like virus (BmPLV), a B. mori atypical protein kinase C (BmaPKC) gene was cloned from larval midgut tissue, expressed in E. coli and purified. Additionally, the BmPLV susceptible silkworm strain and resistant silkworm strain were used to test the effect of the B. mori infection on BmPLV. The result showed that BmaPKC encodes a predicted 586 amino acid protein, which contains a C-terminal kinase domain and an N-terminal regulatory domain. The maximum expression amount of the soluble (His)6-tagged fusion protein was detected after 0.8 mmol/L IPTG was added and cultured at $21^{\circ}C$. The (His) 6-tagged fusion protein revealed about 73 kDa molecular weight which confirmed by western blot and mass spectrography. Furthermore BmaPKC protein were detected at 0-72 h post-infection in BmPLVinfected larval midgut tissue, western blot showed that as time went on, the expression of BmaPKC increased gradually in susceptible strain, the expression quantity on 72 h is 5 times of 0 h. However, in resistant strain, the expression quantity is slightly lower than susceptible strain. But no significant change in resistant strain was observed as time went on. The available data suggest that BmaPKC may involve in the regulation of BmPLV proliferation.

Sphingomonas morindae sp. nov., isolated from Noni (Morinda citrifolia L.) branch

Yao, Su,Ge, Yuanyuan,Zhai, Lei,Liu, Yang,Su, Jiaojiao,Cao, Yanhua,Cheng, Chi,Kim, Song-Gun,Lee, Yong-Jae,Zhang, Xin Microbiology Society 2015 International journal of systematic and evolutiona Vol.65 No.9

Cell-Free Massive MIMO: Zero Forcing and Conjugate Beamforming Receivers

Yao Zhang,Haotong Cao,Meng Zhou,Longxiang Yang 한국통신학회 2019 Journal of communications and networks Vol.21 No.6

In this paper, the uplink performance of cell-free massivemulti-input multi-output (mMIMO) system with the zero-forcing(ZF) and conjugate beamforming (CB) receivers is investigated. Anovel tight approximate rate expression for the ZF receiver is derived,which provides us with a tool for easily quantifying the impactsof the multi-antenna access point (AP), estimation error, pilotcontamination, and power control scheme. Then, leveraging on thetrackable ZF rate expression and the pre-studied CB rate expression,two power control algorithms are proposed. In particular, thefirst algorithm elaborates on maximizing the total rate, subjectingto the quality-of-service (QoS) constraint and each user power constraint. This algorithm utilizes the sequential convex approximationmethod to solve the non-convex issues. In addition, the secondaims at maximizing the rate of one user, in which the remainingusers can meet their QoS constraints. It can be characterized as ageometry programming. Simulation results are provided to showthat the ZF receiver is superior to the CB receiver in terms of sumrate and our proposed algorithms work well in many respects.

Expression profiles of circular RNAs in sheep skeletal muscle

Cao, Yang,You, Shuang,Yao, Yang,Liu, Zhi-Jin,Hazi, Wureli,Li, Cun-Yuan,Zhang, Xiang-Yu,Hou, Xiao-Xu,Wei, Jun-Chang,Li, Xiao-Yue,Wang, Da-Wei,Chen, Chuang-Fu,Zhang, Yun-Feng,Ni, Wei,Hu, Sheng-Wei Asian Australasian Association of Animal Productio 2018 Animal Bioscience Vol.31 No.10

Objective: Circular RNAs (circRNAs) are a newfound class of non-coding RNA in animals and plants. Recent studies have revealed that circRNAs play important roles in cell proliferation, differentiation, autophagy and apoptosis during development. However, there are few reports about muscle development-related circRNAs in livestock. Methods: RNA sequencing analysis was employed to identify and annotate circRNAs from longissimus dorsi of sheep. Reverse transcription followed by real-time quantitative (q) polymerase chain reaction (PCR) analysis verified the presence of these circRNAs. Targetscan7.0 and miRanda were used to analyse the interaction of circRNA-microRNA (miRNA). To investigate the function of circRNAs, an experiment was conducted to perform enrichment analysis hosting genes of circRNAs using gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) pathways. Results: About 75.5 million sequences were obtained from RNA libraries of sheep skeletal muscle. These sequences were mapped to 729 genes in the sheep reference genome. We identified 886 circRNAs, including numerous circular intronic RNAs and exonic circRNAs. Reverse transcription PCR (RT-PCR) and DNA sequencing analysis confirmed the presence of several circRNAs. Real-Time RT-PCR analysis exhibited resistance of sheep circRNAs to RNase R digestion. We found that many circRNAs interacted with muscle-specific miRNAs involved in growth and development of muscle, especially circ776. The GO and KEGG enrichment analysis showed that hosting genes of circRNAs was involved in muscle cell development and signaling pathway. Conclusion: The study provides comprehensive expression profiles of circRNAs in sheep skeletal muscle. Our study offers a large number of circRNAs to facilitate a better understanding of their roles in muscle growth. Meanwhile, we suggested that circ776 could be analyzed in future study.

The Study on the MRTD Measurement System of Medical Infrared Thermal Imager Based on Sub-Pixel Edge Detection Algorithm

Cao Miao,Cui Wenjie,Yao qinghua 보안공학연구지원센터 2016 International Journal of Multimedia and Ubiquitous Vol.11 No.7

MRTD (minimum resolvable temperature difference) is an important parameter to evaluate the infrared thermal imager. MRTD is not only related to the spatial resolution of the system, but also to many factors such as optical system, detector array and so on. The measurement technique and principle of the medical infrared thermal imager’s MRTD is deeply analyzed, and the MRTD measurement model is established in this paper. The MRTD measurement system of infrared thermal imager based on CCD imaging technology is designed. CCD is used to carry on the interpretation of temperature difference in this system instead of the human eye, which can avoid the effect of some factors on the subjective image quality evaluation, such as the contrast, the human interference flaw and so on. The sub-pixel edge detection algorithm is used to deal with the four-bar target image, which can effectively suppress the interface of the random noise on four-bar target image. The experimental results show that the MRTD testing accuracy of the system can reach 0.055℃.