A Genetically Engineered Pseudomonas fluorescens Strain Possesses Dual Activity Against Phytopathogenic Fungi and Insects

( Wen Wei Lu ),( Wei Qiong Zhang ),( Yan Bai ),( Ying Ying Fu ),( Jun Chen ),( Xiao Lu Geng ),( Yu Jing Wang ),( Ming Xiao ) 한국미생물 · 생명공학회 2010 Journal of microbiology and biotechnology Vol.20 No.2

Detection of gas-solid flow faults of a circulating fluidized bed using pressure fluctuations in wind caps

Hua-wei Jiang,Jian-qiang Gao,Hong-wei Chen,Jun-fu Lu,Fu-mao Wang,Yang Wang,Zhen-xin Wu 한국화학공학회 2016 Korean Journal of Chemical Engineering Vol.33 No.7

Wind cap partial blockages and agglomeration are two of the most common gas-solid flow faults that occur under the actual operations of circulating fluidized bed boilers. Using the method of measuring pressure fluctuations, for the characterization of fluid dynamics in fluidized beds, has a great advantage, due to its flexible adaptation to any operating conditions to monitor fluidization. This paper presents research into the use of measuring and analyzing pressure fluctuations in wind caps, for the analysis of the gas-solid fluidization characteristics in a fluidized bed with wind cap partial blockages or agglomeration fault. Partial blockages in a wind cap near feeding side and partial blockages in another wind cap near recycling side as well as agglomeration of different extents were simulated in a cold circulating fluidized bed. Pressure fluctuations in the inlets of several wind caps were measured at different primary air velocities under different fault conditions. They were then analyzed with the methods of statistical average, standard deviation, wavelet analysis and homogeneous index. Based on the calculated characteristic parameters, the effects of gas-solid flow faults on the gas-solid fluidization characteristics were analyzed. Results showed that variations of characteristic parameters of pressure fluctuations were related to variations of the gas-solid flow condition, which were caused by wind cap partial blockages or agglomerations. It is shown that the proposed method is practical.

Study on Interest Coordination Mechanism of Green Supply Chain Based on Dynamic Game

Lu fu-cai,Chen si-hua,He wei 보안공학연구지원센터 2015 International Journal of u- and e- Service, Scienc Vol.8 No.9

Green supply chain which aims at utilizing industrial waste is an effective form of circular economy and practical response to Chinese government’s call for establishment of conservation-oriented society. However, how to coordinate interests of members in green supply chain is a tricky but key problem for the smooth operation of green supply chain. Based on a multi-stage dynamic game model of green supply chain, this paper explores the interest relationship between government and manufacturers, that among manufacturers and that between manufacturers and retailers. Furthermore, this paper `proposes measures and conditions to ensure effective operation of green supply chain and intends to offer references for product pricing of green supply chain, coordination of members of green supply chain as well as governmental environment policy formulation.

Time-dependent Nonlinear Theory and Numerical Simulation of Folded Waveguide Traveling Wave Tubes

Wei Feng Peng,Zhong Hai Yang,Yu Lu Hu,Zan Cao,Yin-Fu Hu,Jin-Jun Feng,Xian-Ping Wu 한국물리학회 2013 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.62 No.6

In this paper, a time-dependent nonlinear theory including the generalized time-dependent radiofrequency (RF) field equations is presented to simulate the beam and wave interaction (BWI) of folded waveguide (FWG) traveling wave tubes (TWTs). The analytical RF fields in FWG TWTs are replaced by digitized RF field profiles obtained from electromagnetic simulations. A W-band FWG TWT is studied by using a self-consistent one-dimensional code based on the theory. The numerical results show good predictions when compared with the experimental tests.

Engineered exosomes enriched in netrin-1 modRNA promote axonal growth in spinal cord injury by attenuating inflammation and pyroptosis

Lu Xiao,Xu Guangyu,Lin Zhidi,Zou Fei,Liu Siyang,Zhang Yuxuan,Fu Wei,Jiang Jianyuan,Ma Xiaosheng,Song Jian 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00

Spinal cord injury (SCI) brings a heavy burden to individuals and society, and there is no effective treatment at present. Exosomes (EX) are cell secreted vesicles containing molecules such as nucleic acids and proteins, which hold promise for the treatment of SCI. Netrin-1 is an axon guidance factor that regulates neuronal growth. We investigated the effects of engineered EX enriched in netrin-1 chemically synthetic modified message RNA (modRNA) in treating SCI in an attempt to find a novel therapeutic approach for SCI.Netrin-1 modRNA was transfected into bone marrow mesenchymal stem cells to obtain EX enriched with netrin-1 (EX-netrin1). We built an inflammatory model in vitro with lipopolysaccharide (LPS) in vitro to study the therapeutic effect of EX-netrin1 on SCI. For experiments in vitro, ELISA, CCK-8 assay, immunofluorescence staining, lactate dehydrogenase release experiments test, real-time quantitative polymerase chain reaction, and western blot were conducted. At the same time, we constructed a rat model of SCI. MRI, hematoxylin-eosin and Nissl staining were used to assess the extent of SCI in rats.In vitro experiments showed that EX had no effect on the viability of oligodendrocytes and PC12 cells. EX-netrin1 could attenuate LPS-induced inflammation and pyroptosis and accelerate axonal/dentritic growth in PC12 cells/oligodendrocytes. In addition, netrin-1 could activate the PI3K/AKT/mTOR signalling pathway upon binding to its receptor unc5b. When Unc5b and PI3K were inhibited, the effect of EX-netrin1 was weakened, which could be reversed by PI3K or mTOR activator. Our in vivo experiments indicated that EX-netrin1 could promote recovery in rats with SCI.We found that EX-netrin1 regulated inflammation, pyroptosis and axon growth in SCI via the Unc5b/PI3K/AKT/mTOR pathway, which provides a new strategy for the treatment of SCI.

A Fast Rough Mode Decision Algorithm for HEVC

Wei-Xin Yao,Dan Yang,Gui-Fu Lu,Jun Wang 한국정보처리학회 2019 Journal of information processing systems Vol.15 No.3

HEVC is the high efficiency video coding standard, which provides better coding efficiency contrasted with theother video coding standard. But at the same time the computational complexity increases drastically. Thirtyfivekinds of intra-prediction modes are defined in HEVC, while 9 kinds of intra prediction modes are definedin H.264/AVC. This paper proposes a fast rough mode decision (RMD) algorithm which adopts the smoothnessof the up-reference pixels and the left-reference pixels to decrease the computational complexity. The three stepsearch method is implemented in RMD process. The experimental results compared with HM13.0 indicate thatthe proposed algorithm can save 39.7% of the encoding time, while Bjontegaard delta bitrate (BDBR) isincreased slightly by 1.35% and Bjontegaard delta peak signal-to-noise ratio (BDPSNR) loss is negligible.

Genetic structure of six cattle populations revealed by transcriptomewide SNPs and gene expression

Wei Wang,Huai Wang,Hui Tang,Jia Gan,Changgeng Shi,Qing Lu,Donghui Fang,Jun Yi,Maozhong Fu 한국유전학회 2018 Genes & Genomics Vol.40 No.7

There are abundant cattle breeds/populations in China, and the systematic discovery of genomic variants is essential for performing the marker assisted selection and conservation of genetic resources. In the present study, we employed whole transcriptome sequencing (RNA-Seq) technology for revealing genetic structure among six Chinese cattle populations according to transcriptome-wide SNPs and gene expression. A total of 68,094 variants consisting of 61,754 SNPs and 6340 InDels were detected and widely distributed among all chromosomes, by which the clear patterns of population structures were revealed. We also found the significantly differential density of variant distribution among genes. Additionally, we totally assembled 15,992 genes and detected obvious differences on the expression profiles among populations. In contrast to genomic variants, the measure of gene expression levels failed to support the expected population structure. Here, we provided a global landscape on the differential expression genes among these cattle populations.

Particle Size and Loading Effects on Particle-Laden Flow with Mono-sized Droplets

( Wei Hsiang Lai ),( Wei Fu Lu ),( Keh Chin Chang ) 한국액체미립화학회 2010 한국액체미립화학회 학술강연회 논문집 Vol.2010 No.-

Turbulence modulation is an interaction between phases that occurs in multiphase flow, and turbulence intensity traditionally can be represented as an indicator of turbulence modulation. Many researchers paid attention on the subject, but no coincident conclusion can be proposed. A general cognition is that larger particles induced wake to enhance the fluid turbulence intensity and smaller particles suppressed the fluid turbulence intensity since the fluid eddy imparted energy to the smaller particle. Here, the authors orderly changed particle size of dispersed phase and mass loading ratio (LR) to study the change on fluid turbulence intensity. Mono-dispersed droplets with a narrow size distribution were used to load into a homogeneous turbulence to investigate the turbulence modulation. Particle Dynamic Analyzer (PDA) was used to measure the particle (droplets and tracers) dynamic information. The particle concentration effect can be significantly observed. After coupling particle concentration and particle wetting area (πd2), a linear relationship can be obtained and it represents the turbulence modulation dominated the dilute two-phase flow as our expectation.

CT Perfusion Imaging Can Predict Patients’ Survival and Early Response to Transarterial Chemo-Lipiodol Infusion for Liver Metastases from Colorectal Cancers

Wei-Fu Lv,Jian-Kui Han,De-Lei Cheng,Chun-Ze Zhou,Ming Ni,Dong Lu 대한영상의학회 2015 Korean Journal of Radiology Vol.16 No.4

To prospectively evaluate the performance of computed tomography perfusion imaging (CTPI) in predicting the early response to transarterial chemo-lipiodol infusion (TACLI) and survival of patients with colorectal cancer liver metastases (CRLM). Computed tomography perfusion imaging was performed before and 1 month after TACLI in 61 consecutive patients. Therapeutic response was evaluated on CT scans 1 month and 4 months after TACLI; the patients were classified as responders and non-responders based on 4-month CT scans after TACLI. The percentage change of CTPI parameters of target lesions were compared between responders and non-responders at 1 month after TACLI. The optimal parameter and cutoff value were determined. The patients were divided into 2 subgroups according to the cutoff value. The log-rank test was used to compare the survival rates of the 2 subgroups. Four-month images were obtained from 58 patients, of which 39.7% were responders and 60.3% were non-responders. The percentage change in hepatic arterial perfusion (HAP) 1 month after TACLI was the optimal predicting parameter (p = 0.003). The best cut-off value was -21.5% and patients who exhibited a ≥ 21.5% decrease in HAP had a significantly higher overall survival rate than those who exhibited a < 21.5% decrease (p < 0.001). Computed tomography perfusion imaging can predict the early response to TACLI and survival of patients with CRLM. The percentage change in HAP after TACLI with a cutoff value of -21.5% is the optimal predictor.

Room temperature ferromagnetism in Ni-doped ZnO films

Deng-Lu Hou,Rui-Bin Zhao,Yan-Yan Wei,Cong-Mian Zhen,Cheng-Fu Pan,Gui-De Tang 한국물리학회 2010 Current Applied Physics Vol.10 No.1

Zn1-xNixO (x = 0.02, 0.03, 0.04, 0.05, 0.07) films were prepared using magnetron sputtering. X-ray diffraction indicates that all samples have a wurtzite structure with c-axis orientation. X-ray photoelectron spectroscopy results reveal that the Ni ion is in a +2 charge state in these films. Magnetization measurements indicate that all samples have room temperature ferromagnetism. In order to elucidate the origin of the ferromagnetism, Zn0.97Ni0.03O films were grown under different atmospheric ratios of argon to oxygen. The results show that as the fraction of oxygen in the atmosphere decreases, both the saturation magnetization and the number of oxygen vacancies increase, confirming that the ferromagnetism is correlated with the oxygen vacancy level.