http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
P206 : Integral hair lipid based barrier in human hair follicle
( Long Quan Pi ),( Sungjoo Tommy Hwang ),( Won Soo Lee ) 대한피부과학회 2014 대한피부과학회 학술발표대회집 Vol.66 No.2
Background: Integral hair lipid (IHL) plays an important role in all compartments of hair and skin substructures and also in involvement of hair development and function. Objectives: In this study, we described the IHL based barrier in human hair follicle according to the hair keratinization. Methods: The techniques of immunofluorescence and of transmission electron microscopy were performed to observe the ultrastructure of the hair lipid. Hair follicles were cut and observed longitudinally along the hair axis. For revealing IHL with electron microscopy, Lee’s fixative, composed of OsO4 and RuO4 was designed and used. Results: Ultrastructure of anagen hair follicle at the level where Henle layers are keratinizing, showed the intercellular lipid layer (IL) and lamellar structure (LS). Ultrastructure of anagen hair follicle at the level where the inner root sheath (IRS) is completely keratinized showed multiple LS and lamellar granules (LG). Multitudes of LS and IL are observed between the keratinized cells in IRS. Conclusion: The IHL in the hair follicle may be regarded as hair barrier to be similar to the epidermal lipid layer functioning as skin barrier.
( Long Quan Pi ),( Sang Yeon Park ),( Sungjoo Tommy Hwang ),( Won Soo Lee ) 대한피부과학회 2013 대한피부과학회 학술발표대회집 Vol.65 No.2
Background: Integral lipid (IL) is bound to the keratinized cell surface to make an environmentally resistant lipid envelope. ILs are synthesized and stored in granules that are known as lamellar granule (LG), which are extruded into the extracellular space in the upper granular layer during cornification. Objectives: To provide baseline data for IHL and LG ultrastructural localization and maturation in the hair follicle. Methods: In this study, we examined distribution and maturation of IHL and LGs in human hair follicle according to the hair keratinization. Results: IHL is mainly positioned on the hair cuticle and inner root sheath (Henle`s layer, Huxley`s layer, inner root sheath cuticle). LG is mainly positioned on the Henle`s layer, Huxley`s layer, while it is not observed on the hair cuticle. Conclusion: This study is considered to provide baseline data for the studies on IHL and LG ultrastructural localization and maturation in the hair follicle, and more studies will be required to examine the roles and the functions of the IHL in the hair biology.
P194 : Expression pattern and role of klotho in human hair follicles
( Xing Hai Jin ),( Long Quan Pi ),( Sungjoo Tommy Hwang ),( Won Soo Lee ) 대한피부과학회 2013 대한피부과학회 학술발표대회집 Vol.65 No.2
Background: Klotho is a newly identified anti-aging protein that plays a pivotal role in regulating ageing. However, whether klotho expresses in human hair follicles (HFs) and whether klotho expression correlates with hair growth have not yet been clearly shown. Objectives: To find the role of Klotho as a regulatory factor for human hair growth Methods: In this study, we examined the expression pattern of klotho in human scalp skin and its role in hair growth. Klotho was expressed in human scalp skin and HF both gene and protein levels. Results: In human scalp skin, prominent klotho expression was observed in the epidermis. Klotho expression in the epidermis was increased with keratinization from basal layer to stratrum coneaum. In human anagen HFs, prominent klotho expression was observed in the epithelium. Klotho expression in the epithelium was increased with keratinization in henle layer and hair cuticle. In human catagen HFs, klotho expression was observed in epithelial strand. Gene transfection of klotho siRNA was markedly inhibited the human hair growth and promoted apoptosis process in organ cultured human follicles. Conclusion: Altogether, these results indicate that klotho might be an important regulatory factor for human hair growth and hair cycle change.
( Seok Yong Ahn ),( Long Quan Pi ),( Sungjoo Tommy Hwang ),( Won Soo Lee ) 대한피부과학회 2012 Annals of Dermatology Vol.24 No.1
Background: Insulin-like growth factor-I (IGF-I) shares a high degree of structural and functional homology with insulin and is a potent mitogen supporting cell growth and survival in many kinds of the tissues and cells. It also plays a role in some differentiation and anti-apoptotic functions. In previous reports, it has been shown that IGF-I stimulates hair follicle (HF) growth, maintains the anagen stage, and postpones the catagen stage. Objective: The exact mechanism of the effect of IGF-I on HF growth is not yet established. Therefore, we investigated the relationships between IGF-I and various other factors (i.e. apoptosis related molecules, pro-inflammatory cytokines, other growth factors, etc.) in the control of HF growth. Methods: The effect of IGF-I on human hair growth was measured using an organ culture model of human HFs and compared with a control group that did not receive IGF-I. We also measured mRNA expression of factors related to hair growth and apoptosis (which was determined by reverse transcription polymerase chain reaction (RT-PCR). RT-PCR was done on days 2, 4, 6, and 8 of organ culture. Results: In organ cultured human hair follicles, IGF-I had a positive effect on the rate of linear hair growth. IGF-I maintained the anagen phase. IGF-I increased the expression of platelet-derived growth factor (PDGF)-A, PDGF-B and the expression ratio of Bcl-2/Bax. Conclusion: The effect of IGF-I on hair growth appears to be related to the upregulation of PDGF-A and PDGF-B and to the anti-apoptotic effect of IGF-I. (Ann Dermatol 24(1) 26∼31, 2012)
Keum, Dong In,Pi, Long-Quan,Hwang, Sungjoo Tommy,Lee, Won-Soo The Korean Society of Ginseng 2016 Journal of Ginseng Research Vol.40 No.2
Background: Chemotherapy-induced alopecia (CIA) is one of the most distressing side effects for patients undergoing chemotherapy. This study evaluated the protective effect of Korean Red Ginseng (KRG) on CIA in a well-established in vitro human hair follicle organ culture model as it occurs in vivo. Methods: We examined whether KRG can prevent premature hair follicle dystrophy in a human hair follicle organ culture model during treatment with a key cyclophosphamide metabolite, 4-hydroperoxycyclophosphamide (4-HC). Results: 4-HC inhibited human hair growth, induced premature catagen development, and inhibited proliferation and stimulated apoptosis of hair matrix keratinocytes. In addition, 4-HC increased p53 and Bax protein expression and decreased Bcl2 protein expression. Pretreatment with KRG protected against 4-HC-induced hair growth inhibition and premature catagen development. KRG also suppressed 4-HC-induced inhibition of matrix keratinocyte proliferation and stimulation of matrix keratinocyte apoptosis. Moreover, KRG restored 4-HC-induced p53 and Bax/Bcl2 expression. Conclusion: Overall, our results indicate that KRG may protect against 4-HC-induced premature catagen development through modulation of p53 and Bax/Bcl2 expression.
P207 : Pro-apoptotic mechanism of TGF-β in human hair follicle epithelial cells
( Long Quan Pi ),( Xing Hai Jin ),( Sungjoo Tommy Hwang ),( Won Soo Lee ) 대한피부과학회 2014 대한피부과학회 학술발표대회집 Vol.66 No.2
Background: Transforming growth factor-β (TGF-β) is a multifunctional growth factor that regulates cell growth, differentiation and apoptosis in various cell types. It has been shown that TGF-β plays an important role in the induction of catagenduring the hair cycle. However, the molecular mechanism of TGF-β action onepithelial cell growth of hair follicle has not yet been clearly elucidated. Objectives: In the present study, we investigated the effects of exogenous TGF-β on cultured human follicular keratinocytes (FKs). Results: Exogenous TGF-β induced FK apoptosis through caspase-3 activation which was related to elevated reactive oxygen species (ROS) levels. A TGF-β receptor I blocker, SB431542 or antioxidant (NAC), reversed the changes triggered by TGF-β. Conclusion: These data suggest that TGF-β induced apoptosis is mediated by ROS in hair follicle FKs.
Dong In Keum,Long-Quan Pi,Sungjoo Tommy Hwang,이원수 고려인삼학회 2016 Journal of Ginseng Research Vol.40 No.2
Background: Chemotherapy-induced alopecia (CIA) is one of the most distressing side effects for patients undergoing chemotherapy. This study evaluated the protective effect of Korean Red Ginseng (KRG) on CIA in a well-established in vitro human hair follicle organ culture model as it occurs in vivo. Methods: We examined whether KRG can prevent premature hair follicle dystrophy in a human hair follicle organ culture model during treatment with a key cyclophosphamide metabolite, 4-hydroperoxycyclophosphamide (4-HC). Results: 4-HC inhibited human hair growth, induced premature catagen development, and inhibited proliferation and stimulated apoptosis of hair matrix keratinocytes. In addition, 4-HC increased p53 and Bax protein expression and decreased Bcl2 protein expression. Pretreatment with KRG protected against 4-HC-induced hair growth inhibition and premature catagen development. KRG also suppressed 4-HCinduced inhibition of matrix keratinocyte proliferation and stimulation of matrix keratinocyte apoptosis. Moreover, KRG restored 4-HC-induced p53 and Bax/Bcl2 expression. Conclusion: Overall, our results indicate that KRG may protect against 4-HC-induced premature catagen development through modulation of p53 and Bax/Bcl2 expression.