Polymorphisms of TERT and CLPTM1L and the Risk of Hepatocellular Carcinoma in Chinese Males

Su, Ling-Yan,Li, Xue-Lian,Shen, Li,Zhang, Yue,Zhao, Meng-Meng,Yin, Zhi-Hua,Su, Hong-Ying,Zhou, Bao-Sen Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.19

Background: Telomerase reverse transcriptase (TERT) and cleft lip and palate trans-membrane 1 like (CLPTM1L) genes located on chromosome 5p15.33 are known to influence the susceptibility to various cancers. Here, we examined the association of TERT and CLPTM1L single nucleotide polymorphisms (SNPs) with hepatocellular carcinoma (HCC). Materials and Methods: Genotyping of TERT SNP rs2736098 and CLPTM1L SNP rs401681 was performed using TaqMan allelic discrimination assays in a case-control study of 201 HCC cases and 210 controls in a Chinese male population. Odds ratios (ORs) and 95% confidence intervals (CIs) were estimated using logistic regression analyses. Results: Both the rs2736098 T allele of TERT and the rs401681 T allele of CLPTM1L were associated with a significantly increased risk of HCC (adjusted odds ratio [OR]=1.605, 95% confidence interval [CI]=1.164-2.213; adjusted OR=1.399, 95%CI=1.002-1.955, respectively). Individuals carrying both TERT and CLPTM1L risk genotypes had an even higher risk of HCC (adjusted OR=4.420, 95%CI= 2.319-8.425). The TERT rs2736098 T allele was also significantly associated with the level of the HCC clinical indicator alpha-fetoprotein (P=0.026). Conclusions: Our results show that genetic variants of TERT and CLPTM1L may contribute to HCC susceptibility in Chinese males.

SIRT7 Exhibits Oncogenic Potential in Human Ovarian Cancer Cells

Wang, Hong-Ling,Lu, Ren-Quan,Xie, Su-Hong,Zheng, Hui,Wen, Xue-Mei,Gao, Xiang,Guo, Lin Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.8

Background: Sirtuin7 (SIRT7) is a type of nicotinamide adenine dinucleotide oxidized form (NAD+)-dependent deacetylase and the least understood member of the sirtuins family; it is implicated in various processes, such as aging, DNA damage repair and cell signaling transduction. There is some evidence that SIRT7 may function as a tumor trigger for human malignancy. Here, we aimed to explore the biological function of SIRT7 in ovarian carcinoma cells and its potential mechanism. Materials and Methods: Expression of SIRT7 in ovarian cancer cell lines was detected by western blotting. Transduced cell lines with SIRT7 knockdown or overexpression were constructed. Cell viability, cologenic, apoptosis-associated and motility assays were performed to elucidate the biological function of SIRT7 in ovarian cancer cells. Results: SIRT7 demonstrated a higher level in ovarian cancer cell lines compared with normal cells. On the one hand, down-regulation of SIRT7 significantly reduced ovarian cancer cell growth, repressed colony formation and increased cancer cell apoptosis; on the other hand, up-regulation promoted the migration of cancer cells. Additionally, repression of SIRT7 also induced change in apoptosis-related molecules and subunits of the NF-${\kappa}B$ family. Conclusions: In the present study, our data indicated that SIRT7 might play a role of oncogene in ovarian malignancy and be a potential therapeutic target.

Tumor suppressive function of NQO1 in cutaneous squamous cell carcinoma cells

( Su-hyuk Yim ),( Qing-ling Zhang ),( Dongkyun Hong ),( Kyoung Eun Jung ),( Chong-won Choi ),( Young Lee ),( Chang-deok Kim ),( Young-joon Seo ) 대한피부과학회 2019 대한피부과학회 학술발표대회집 Vol.71 No.2

Background: Cutaneous squamous cell carcinoma (SCC) is an easily occurred cancer, which can worsen the quality of life considerably. It is known that external stimulus induces cutaneous SCC via provoking oxidative stress. NAD(P)H dehydrogenase 1 (NQO1) has functions as a guardian against oxidative stress. However, the effect of NQO1 on cutaneous SCC is not clearly elucidated. Objectives: In this study, we investigated the effect of NQO1 on cutaneous SCC cells. Methods: To examine the effects of NQO1, we transduced SCC lines (SCC12 and SCC13) with the NQO1 expressing or knockdown adenovirus. Results: Overexpression of NQO1 resulted in significant decrease of cell proliferation and colony forming activity of SCC lines. By contrast, knockdown of NQO1 increased the cell proliferation and colony forming activity. Accordingly, the levels of proliferation-related regulators, such as CDK4, CDK6, SOX2 and p63, were decreased by overexpression of NQO1, while those were increased by knockdown of NQO1. In addition, NQO1 affected the invasion and migration of SCC cells in a very similar way, with the regulation of epithelial-mesenchymal transition (EMT)-related molecules, including E-cadherin, N-cadherin, Vimentin, Snail and Slug. Finally, overexpression of NQO1 decreased the level of phosphorylated AKT, JNK and p38 MAPK, while knockdown of NQO1 increased the level of phosphorylated signaling molecules. Conclusion: Based on these data, NQO1 has tumor suppressive function in cutaneous SCC cells.

Inhibition of poly(I:C)-induced inflammation by salvianolic acid A in skin keratinocytes

( Su-hyuk Yim ),( Qing-ling Zhang ),( Xue Mei Li ),( Jin Gwi Yoo ),( Dong-kyun Hong ),( Jin-hyup Lee ),( Chong Won Choi ),( Kyung Duck Park ),( Young Lee ),( Chang Deok Kim ),( Young-joon Seo ),( Jeun 대한피부과학회 2018 대한피부과학회 학술발표대회집 Vol.70 No.2

Background: Skin keratinocytes participate actively in inducing immune responses when external pathogens are introduced, thereby contributing to elimination of pathogens. However, in condition where the excessive inflammation is occurred, chronic skin disease such as psoriasis can be provoked. Objectives: We tried to screen the putative therapeutics for inflammatory skin disease, and found that salvianolic acid A (SAA) has an inhibitory effects on keratinocyte inflammatory reaction. The aim of this study is to demonstrate the effects of SAA in poly(I:C)-induced inflammatory reaction in skin keratinocytes. Methods: The keratinocytes were pretreated with SAA then stimulated with poly(I:C). Inflammatory reaction of keratinocytes, then we verified using RT-PCR, ELISA and Western blot. Results: When skin keratinocytes were pre-treated with SAA, it significantly inhibited poly(I:C)-induced expression of inflammatory cytokines including IL-1β, IL-6, IL-8, TNF-α, and CCL20. SAA inhibited poly(I:C)-induced activation of NF-κB signaling. And SAA also inhibited inflammasome activation, evidenced by decrease of IL-1β secretion. Finally, SAA markedly inhibited poly(I:C)-induced NLRP3 expression. Conclusion: These results demonstrate that SAA has an inhibitory effect on poly(I:C)-induced inflammatory reaction of keratinocytes, suggesting that SAA can be developed for the treatment of inflammatory skin diseases such as psoriasis.

한국어 교사용 지도서 개발의 쟁점과 실제 적용에 관한 연구 -제3국 출생 탈북 아동을 위한 한국어 교재 『돋움한국어』의 교사용 지도서를 중심으로-

홍혜란 ( Hong Hye-ran ),유소영 ( Yoo So-young ),최수정 ( Choi Su-jeong ),김령 ( Jin Ling ) 국제어문학회(구 국제어문학연구회) 2021 국제어문 Vol.- No.89

본고는 교사용 지도서 개발에 관한 연구로 제3국 출생 탈북 아동 대상 한국어 교재의 활용도를 높이고 교사의 전문성을 강화하기 위한 목적으로 시작되었다. 이를 위해 그간 이루어진 한국어 지도서 개발과 관련한 선행연구를 검토하고, 이 중 요구분석 연구의 성과를 집중 분석하여 그 결과를 실제 지도서 개발의 기초 자료로 삼을 수 있도록 하였다. 아울러 다문화 가정 아동, 재외동포 아동, KSL 초, 중, 고등학생 등 다양한 변인을 대상으로 한 지도서의 개발 사례를 분석하였다. 분석 결과를 바탕으로 지도서 개발과 관련한 쟁점을 지도서의 구성 체제와 내용으로 나누어 살펴보고, 교사용 지도서의 개발 방향을 모색하고 원리를 설정하였다. 이에 따라 본 연구에서는 제3국 출생 탈북 아동 대상 한국어 교재의 교사용 지도서 개발 원리를 첫째, 수업 준비와 운영의 흐름을 고려한 구성 체제, 둘째, 아동 학습자의 특성을 고려한 수업 활동 구성, 셋째, 효율적인 수업 준비를 돕기 위한 내용의 이원화, 넷째, 대조분석을 바탕으로 한 학습자 오류 클리닉 제공, 다섯째, 수업 운영의 편의성과 활용도 제고를 위한 수업 보조 자료 개발의 다섯 가지로 설정하였다. 나아가 실제 지도서에서의 적용 예시를 보였다. 이 연구는 교사의 요구분석 내용을 지도서에 반영하고 지도서 개발과 관련한 쟁점을 검토함으로써 교사, 학습자 변인을 고려한 지도서 체계와 내용을 마련한 교사용 지도서의 실제를 보여줬다는 데 의의가 있다. 이는 한국어 교재의 활용도를 높이고 교사의 전문성을 강화하는 데 기여할 것으로 기대된다. This study aims to develop a teacher’s guidebook to improve the utilization of Korean textbooks for children of North Korean defectors. This guidebook is also intended to strengthen the professionalism of teachers. Before developing an actual teacher’s guidebook, this paper reviewed previous relevant studies to collect results applicable to the development of a teacher’s guidebook. In addition, the various factors that a teacher’s guidebook must address were analyzed, including multicultural children; overseas Korean children; and elementary, middle school, and high school KSL students. Based on the results of the analysis, the development of a teacher’s guidebook was examined from the perspective of composition, and the contents and principles necessary for teachers’ guidebooks were established. The following are five principles for developing a teacher’s guidebook for children born in third countries to North Korean defectors based on this study: 1) a system of composition considering the flow of class preparation and management; 2) class activities that consider the characteristics and needs of child learners; 3) dualization of contents to help efficient class preparation; 4) providing learner error clinics based on contrast analysis; and 5) development of class materials for the enhancement of convenience and utilization in classroom operation. Furthermore, this paper shows examples of applications in the teacher’s guidebook. The significance of the study is that it shows the practical application of a teacher’s guidebook based on a system and contents that consider both teachers and learners’ variables. It is hoped that this study and the guidebook that it created will enhance Korean textbooks and strengthen the professionalism of teachers.

Aberrant Methylation of Genes in Sputum Samples as Diagnostic Biomarkers for Non-small Cell Lung Cancer: a Meta-analysis

Wang, Xu,Ling, Li,Su, Hong,Cheng, Jian,Jin, Liu Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.11

Background: We aimed to comprehensively review the evidence for using sputum DNA to detect non-small cell lung cancer (NSCLC). Materials and Methods: We searched PubMed, Science Direct, Web of Science, Chinese Biological Medicine (CBM), Chinese National Knowledge Infrastructure (CNKI), Wanfang, Vip Databases and Google Scholar from 2003 to 2013. The meta-analysis was carried out using a random-effect model with sensitivity, specificity, diagnostic odd ratios (DOR), summary receiver operating characteristic curves (ROC curves), area under the curve (AUC), and 95% confidence intervals (CI) as effect measurements. Results: There were twenty-two studies meeting the inclusion criteria for the meta-analysis. Combined sensitivity and specificity were 0.62 (95%CI: 0.59-0.65) and 0.73 (95%CI: 0.70-0.75), respectively. The DOR was 10.3 (95%CI: 5.88-18.1) and the AUC was 0.78. Conclusions: The overall accuracy of the test was currently not strong enough for the detection of NSCLC for clinical application. Dscovery and evaluation of additional biomarkers with improved sensitivity and specificity from studies rated high quality deserve further attention.

Cloning and analysis of b-amyrin synthase gene in Bupleurum chinense

Ke Gao,Su-rui Wu,Ling Wang,Yan-hong Xu,Jian-he Wei,Chun Sui 한국유전학회 2015 Genes & Genomics Vol.37 No.9

Bupleurum chinense DC. is one of the source plants of a well-known crude drug, Chai hu (Radix Bupleuri), producing triterpenoid saponins (saikosaponins) with a wide-spectrum of pharmacological applications. The biosynthesis of triterpenoid saponins involved with the cyclizing of the precursor 2,3-oxidosqualene to produce the first committed triterpene b-amyrin catalyzed by b-amyrin synthase (b-AS), whereafter diverse of triterpenoid saponins was biosynthesized. In addition, 2,3-oxidosqualene could be catalyzed by cycloartenol synthase directing to the synthesis of phytosterol. b-AS was thus defined as an important branch point between primary and secondary metabolisms, and may play a regulating role in the control of triterpenoid saponins biosynthesis. In this study, the promoter and protein-encoding regions of a b-AS gene (designated bcAS1) were isolated by genome walking and PCR from B. chinense. Several important cis-acting elements for gene regulation were identified within the promoter region including light-responsive, hormoneresponsive and various other stress-related elements. Approximate 0.8 kb fragment on upstream of ATG start codon of bcAS1 was sub-cloned into pAN580 vector to replace the 35S promoter driving the expression of green fluorescent protein (GFP) gene. The promoter activity was detected by transient expression in onion epidermis cells by the expression of GFP. Approximately 6 kb length of bcAS1 gene was cloned, containing 18 exons and 17 introns. Although a dozen of b-AS cDNA was isolated, seldom the promoter and gene of it was reported. This work was a valuable foundation for further studies on the regulatory role of b-AS in biosynthesis of saikosaponins.

Vitamin D3 and Beta-carotene Deficiency is Associated with Risk of Esophageal Squamous Cell Carcinoma - Results of a Case-control Study in China

Huang, Gui-Ling,Yang, Lei,Su, Ming,Wang, Shao-Kang,Yin, Hong,Wang, Jia-Sheng,Sun, Gui-Ju Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.2

Objective: The aim was to evaluate roles of vitamin D3 (VD3) and beta-carotene (BC) in the development of esophageal squamous cell carcinoma (ESCC) in a high-risk area, Huai'an District, Huai'an City, China. Methods: 100 new ESCC diagnosed cases from 2007 to 2008 and 200 residency- age-, and sex-matched healthy controls were recruited. Data were collected from questionnaires, including a food frequency questionnaire (FFQ) to calculate the BC intake, and reversed phase high-performance liquid chromatography (RP-HPLC) was used to measure the serum concentrations of BC and VD3. Odds ratios (OR) and 95% confidence intervals (CI) were calculated in conditional logistic regression models. Results: The average dietary intake of BC was $3322.9{\mu}g$ (2032.4-5734.3) in the case group and $3626.8{\mu}g$ (1961.9-5827.9) in control group per capita per day with no significant difference by Wilcoxon test (p>0.05). However, the levels of VD3 and BC in the case group were significantly lower than in the control group (p<0.05). The OR values of the highest quartile and the lowest quartile of VD3 and BC in serum samples were both 0.13. Conclusion: Our results add to the evidence that high circulating levels of VD3 and BC are associated with a reduced risk of ESCC in this Chinese population.

Apoptosis in response to heat stress is positively associated with heat-shock protein 90 expression in chicken myocardial cells in vitro

Xiao-Hui Zhang,Hong Wu,Shu Tang,Qiao-Ning Li,Jiao Xu,Miao Zhang,Ya-Nan Su,Bin Yin,Qi-Ling Zhao,Nicole Kemper,Joerg Hartung,Endong Bao 대한수의학회 2017 Journal of Veterinary Science Vol.18 No.2

To determine heat-shock protein (Hsp)90 expression is connected with cellular apoptotic response to heat stress and its mechanism, chicken (Gallus gallus) primary myocardial cells were treated with the Hsp90 promoter, aspirin, and its inhibitor, geldanamycin (GA), before heat stress. Cellular viability, heat-stressed apoptosis and reactive oxygen species level under different treatments were measured, and the expression of key proteins of the signaling pathway related to Hsp90 and their colocalization with Hsp90 were detected. The results showed that aspirin treatment increased the expression of protein kinase B (Akt), the signal transducer and activator of transcription (STAT)-3 and p-IKKa/b and the colocalization of Akt and STAT-3 with Hsp90 during heat stress, which was accompanied by improved viability and low apoptosis. GA significantly inhibited Akt expression and p-IKKa/b level, but not STAT-3 quantity, while the colocalization of Akt and STAT-3 with Hsp90 was weakened, followed by lower cell viability and higher apoptosis. Aspirin after GA treatment partially improved the stress response and apoptosis rate of tested cells caused by the recovery of Akt expression and colocalization, rather than the level of STAT-3 (including its co-localization with Hsp90) and p-IKKa/b. Therefore, Hsp90 expression has a positive effect on cellular capacity to resist heat-stressed injury and apoptosis. Moreover, inhibition of Hsp90 before stress partially attenuated its positive effects.

Absence of EZH2 Gene Mutation in Chronic Myeloid Leukemia Patients in Blast Crisis

Chen, Hao-Yue,Yao, Hong,Wu, Ling-Yu,Liu, Can-Jun,Zhu, Jian-Qin,Liu, Chun-Hua,Wang, Wei,Dong, Sha-Sha,Ping, Na-Na,Chen, Su-Ning,Sun, Miao Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.5