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UV , TPA , heterologous DNA inserts 가 동물세포에서 homologous recombination 에 미치는 영향
송규영,Kucherlapati, Raju 한국유전학회 1992 Genes & Genomics Vol.14 No.1
We have studied the plasmid x plasmid recombination system to examine factors that might increase the frequency of homologous recombination. Treatment of input DNA with UV doses up to 100 Jm^(-2) did not have a significant effect on recombination, on the other hand, treating the recipient EJ cells with UV light at a dose of 1 Jm^(-2) prior to transfection resulted in a two fold increase in recombination. The tumor promoter TPA treatment did not have an effect on recombination or transformation. We have studied the effect of heterologous inserts on homologous recombination and observed that as the length of insert increased, there was a decline in the frequency of recombination.
In vivo wide-area cellular imaging by side-view endomicroscopy
Kim, Pilhan,Chung, Euiheon,Yamashita, Hiroshi,Hung, Kenneth E,Mizoguchi, Atsushi,Kucherlapati, Raju,Fukumura, Dai,Jain, Rakesh K,Yun, Seok H Nature Publishing Group 2010 NATURE METHODS Vol.7 No.4
In vivo imaging of small animals offers several possibilities for studying normal and disease biology, but visualizing organs with single-cell resolution is challenging. We describe rotational side-view confocal endomicroscopy, which enables cellular imaging of gastrointestinal and respiratory tracts in mice and may be extensible to imaging organ parenchyma such as cerebral cortex. We monitored cell infiltration, vascular changes and tumor progression during inflammation and tumorigenesis in colon over several months.