Genetic aberrations on the short arm of chromosome 8 (8p) in tongue carcinomas

Akiyuki Murano,Kanae Ono,Hirofumi Koike,Yosuke Endo,Ken Shimada,Kenshi Kawasaki,Hitomi Nomura,Masashi Shiiba,Katsuhiro Uzawa,Hideki Tanzawa 대한구강악안면외과학회 2016 대한구강악안면외과학회지 Vol.38 No.2

Aberrations on the short arm of chromosome 8 (8p) are frequently observed in several human cancers. In this study, 20 squamous cell carcinoma (SCC) specimens from the tongue were examined in order to evaluate the role of 8p in SCC of the tongue. Microsatellite analysis using 14 markers demonstrated two commonly deleted regions (CDRs) on 8p. Reverse transcription-polymerase chain reaction (RT-PCR) revealed frequent downregulation of the FEZ1 gene, mapped to 8p22, and frequent over-expression of the cathepsin B gene, mapped to 8p-21-22. These results suggested that genetic aberrations are involved in the development of SCC of the tongue. However, no significant relationship was observed to be established between the genetic alterations and clinicopathological features. Thus, further investigation is necessary in order to clarify the clinical role of 8p in carcinoma of the tongue.

A purine-type heat shock protein 90 inhibitor promotes the heat shock response in Arabidopsis

Hiroki Murano,Takumi Matsubara,Ikuo Takahashi,Masakazu Hara 한국식물생명공학회 2017 Plant biotechnology reports Vol.11 No.2

Geldanamycin is a macrocyclic heat shock protein 90 (HSP90) inhibitor that suppresses cancer cell proliferation. Since geldanamycin also promotes the heat shock response (HSR) in cells, this compound is used as a chemical inducer of the HSR in Arabidopsis. Although many types of HSP90 inhibitors that are different from the macrocyclic types have been developed in pharmaceutical research, non-macrocyclic HSP90 inhibitors have not been investigated in terms of whether they can induce the HSR in plants. Here, we determined the HSR-inducing activities in Arabidopsis of 10 non-macrocyclic HSP90 inhibitors including 2 benzamide derivatives, 3 purine derivatives, and 5 resorcinol derivatives. Among the tested inhibitors, PU-H71, which is a purine derivative, showed the highest HSR-inducing activity. The activity of PU-H71 was significantly higher than that of geldanamycin. The application of PU-H71 induced the HSR in all Arabidopsis seedlings. The HSP17.6C-CI and HSP70 proteins accumulated after the treatment with PU-H71. The seedlings treated with PU-H71 maintained more chlorophyll than the control seedlings after the heat stress. These results suggest that the purine-derivative HSP90 inhibitor PU-H71 enhanced the heat tolerance of Arabidopsis by promoting the HSR in the plant.

Characteristics on night-time waste collection and transportation in Fukuoka city, Japan

( Yoko Murano ),( Shinya Suzuki ),( Ayako Tachifuji ) 한국폐기물자원순환학회(구 한국폐기물학회) 2018 한국폐기물자원순환학회 심포지움 Vol.2018 No.1

Effects of Acute Pre-Workout Dietary Supplement Ingestion With and Without Synephrine on Serum Creatine

( Yp Jung ),( M Koozehchian ),( R Dalton ),( C Rasmussen ),( P Murano ),( Cp Earnest ),( Rb Kreider ) 한국체육학회 2016 국제스포츠과학 학술대회 Vol.2016 No.1

Purpose: A number of nutritional strategies have been developed to optimize nutrient delivery prior to exercise. As a result, various pre-workout supplements have been developed to increase energy availability, promote vasodilation, and/or positively affect exercise capacity. The purpose of this study was to examine the effects of acute pre-workout dietary supplement ingestion with and without synephrine on serum creatine in healthy, active volunteers. Method: We randomized 25 apparently healthy and recreationally active men (n=20) and women (n=5) (21.76±3.00 yr, 15.24±5.26% fat, 25.09±3.03 kg/m2) in a double-blind, crossover, randomized and placebo- controlled manner to acutely ingest (1) a dextrose flavored placebo (PLA); (2) a pre-workout supplement (PWS) containing 3 g beta alanine, 2 g creatine nitrate, 2 g arginine AKG, 300 mg N-acetyl tyrosine, 270 mg caffeine, 15 mg Mucuna pruriens; or, (3) PWS + 20 mg synephrine (PWS+S). Initial venous blood serum collections followed 10-12 hours of fasting, with a second and third collection 30-min and 90-min following a pre-workout supplement ingestion. Participants repeated the experiment after a one-week washout period with the alternate supplements in a randomized and counterbalanced manner. Plasma was subsequently extracted and stored at -80°C, and then analyzed by calorimetric assay kits to measure serum creatine level. Data presented mean ± SD and mean change ± 95% CI. Data were analyzed by two-way repeated measure ANOVA and one-way repeated measure ANOVA. Result: We observed significant effects for treatment (p<0. 001), time (p<0. 001), and the treatment x time interaction (p<0.001) on serum creatine. Overall, significant differences for creatine were observed for PWS+S (2.62 μmol/L; 95% CI, 2.29, 2.94) and PWS (2.31 μmol/L; 95% CI, 1.97, 2.65) vs. PLA (0.71 μmol/L; 95% CI, 0.63, 0.80). The effect also showed significant differences at 30-min (2.37 μmol/L; 95% CI, 2.02, 2.71) and 90-min (2.57 μmol/L; 95% CI, 2.14, 2.99) from pre-ingestion (0.71 μmol/L; 95% CI, 0.63, 0.78). Treatment x time interaction showed significant increases in serum creatine levels for PWS+S (3.28 μmol/L; 95% CI, 2.80, 3.76) and PWS (3.13 μmol/L; 95% CI, 2.52, 3.74) vs. PLA (0.69 μmol/L; 95% CI, 0.60, 0.78) at 30-min, and PWS+S (3.89 μmol/L; 95% CI, 3.21, 4.57) and PWS (3.10 μmol/L; 95% CI, 2.48, 3.71) vs. PLA (0.71 μmol/L; 95% CI, 0.61, 0.82) at 90-min of supplement ingestion. Although there was no significant difference between PWS+S and PWS (p=0.48) at 30-min post-ingestion, at 90-min post-ingestion, serum creatine was significantly increased (p<0.001) in PWS+S subjects (0.79 μmol/L; 95% CI, 0.37, 1.21) compared to PWS subjects. Area under the curve (AUC) analysis also showed a significant difference between treatments (p<0.001). Post-hoc comparisons demonstrated significant differences between PWS+S (5.57 μmol/L; 95% CI, 4.88, 6.25) and PWS (5.50 μmol/L, 95% CI, 4.26, 5.82) vs. PLA (1.42 μmol/L; 95% CI, 1.25, 1.59) for 2-hrs. PWS+S (0.53 μmol/L; 95% CI, 0.02, 1.04) vs. PWS was also showed significance throughout testing. Conclusion: The acute ingestion of a dietary PWS containing beta alanine, creatine nitrate, arginine AKG, N-acetyl tyrosine, caffeine, and Mucuna pruriens increased serum creatine levels 2-hr post-ingestion compared to PLA and the addition of synephrine enhanced this effect.

Influence of continental outflow events on the aerosol composition at Cheju Island,South Korea

Park, John K.,Arimoto, Richard,Savoie, Dennis,Carmichael, Gregory R,Prospero, Joseph,Hong, Min-Sun,Ueda, Hiromasa,Murano, Kentaro,Chen, Li-Ling,Kim, Y. P.,Shim, Shang,Lee, Ho-geun,Song, Chul H.,Kang, 濟州大學校 基礎科學硏究所 1998 基礎科學硏究 Vol.11 No.1

The chemical composition of aerosols measured at Cheju Island, Korea, over the 3-year period March 1992 to February 1995 are presented and discussed, with a particular emphasis on the Pacific Exploratory Mission in the Western Pacific (PEM-West B) time period. Cheju Island is under the influence of continental outflow conditions nearly 70% of the year, and as a result the aerosol loading of sea salt as well as continental aerosol components is high. The 3-year mean values derived from the daily tape filters are non-sea-salt (nss) SO₄²?=6.8㎍/㎥, NO₃?=1.2㎍/㎥, Cl??=1.9㎍/㎥, Na+=1.7㎍/㎥, Ca²?=0.5㎍/㎥, NH₄?=1.3㎍/㎥, Mg²?=0.3㎍/㎥, and K+=0.4㎍/㎥. Sea-salt components show peak values in winter, while calcium, nitrate, potassium, and to a lesser extent, sulfate and ammonium, show higher values in the spring, and all species exhibit a pronounced minimum in summer. Trajectory and principal component analysis show that elevated levels of primary aerosols (both sea salt and soil-derived) occur with strong wind conditions associated with winter and spring, and high concentrations of non-sea-salt components are most strongly associated with springtime continental outflow events. During the PEM-West B period, nitrate and calcium are found to be ∼60% higher than the annual mean, sea-salt components ∼30% higher, and sulfate ∼10% higher. The aerosol data are combined with gaseous SO₂ concentrations, precipitation chemistry data and companion aerosol measurements taken at the same site, to provide further insights into the aerosol composition at Cheju. At Cheju, sulfate and ammonium are found mostly in the fine fraction (∼80 to 90%), while calcium and nitrate reside in the coarse size fraction. sulfate and nitrate are not associated with the primary aerosols, but rather become associated with the aerosol during the long-range transport process.

Aerosol composition at Cheju Island, Korea

Kim, Young,Kang, C.,Lee, Hogeun,Park, John K.,Hong, Min-Sun,Shim, Shang,Chen, Li-Ling,Carmichael, Gregory R.,Ueda, Hiromasa,Murano, Kentaro 濟州大學校 基礎科學硏究所 1997 基礎科學硏究 Vol.10 No.1

The seasonal variation in chemical composition of aerosols measured at Cheju Island, Korea, is presented and discussed. Daily tape filters have been obtained for the three year period analyzed, March 1992 through February 1995. The annual average non-sea-salt sulfate and citrate mass concentrations of aerosols measured at Cheju, are 7.2μg/㎥ and 1.2μg/㎥, respectively; while calcium and potassium are present in nearly equal amounts of ∼0.5μg/㎥, and ammonium averages 1.3μg/㎥. The annual mean mass ratios are ?? and ?? Each species is found to follow a seasonal cycle, with sulfate, citrate, ammonium, potassium, and calcium all showing higher values in the spring. In contrast, chlorine concentrations are 10% lower in the spring. Aerosol enrichment factors relative to the composition of seawater and crustal material from the dust-source regions in central China reveal that the aerosol measured at Cheju is enriched in sulfate, nitrate, and ammonium, but is deficient in chlorine (relative to seawater). The data from the daily tape filters are also compared with those from weekly Hi-Vol filters located at the same site and other measurements in the region.