Substance P reduces apoptotic cell death possibly by modulating the immune response at the early stage after spinal cord injury

Jiang, Mei Hua,Lim, Ji Eun,Chi, Guang Fan,Ahn, Woosung,Zhang, Mingzi,Chung, Eunkyung,Son, Youngsook Wolters Kluwer Health | Lippincott Williams Wilkin 2013 NEUROREPORT - Vol.24 No.15

Previously, we have reported that substance P (SP) enhanced functional recovery from spinal cord injury (SCI) possibly by the anti-inflammatory modulation associated with the induction of M2-type macrophages at the injured lesion. In this study, we explored the cytokine expression profiles and apoptotic cell death in the lesion site of the SCI after an immediate intravenous injection of SP. SP injection increased the levels of interleukin-4 (IL-4), IL-6, and IL-10 at day 1 after the SCI approximately by 2-, 9-, and 10-folds when compared with the control SCI, respectively. On the basis of double immunofluorescence staining with IL-10 and CD11b, activated macrophages or microglia expressing IL-10 appeared in the margin of the lesion site at day 1 only after the SP injection. This SP-mediated alteration in the lesion microenvironment was shown to be associated with the lower cell death of neuronal cells at day 1 and oligodendrocytes at day 5 by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay, which was also accompanied by a decrease in caspase-3 activation. These findings suggest that SP may reduce the inflammation-induced secondary cell death, possibly through immune modulation at an early stage after the SCI.

Anti-toxoplasmosis Effects of Oleuropein Isolated from <i>Fraxinus rhychophylla</i>

Jiang, Jing-Hua,Jin, Chun-Mei,Kim, Youn-Chul,Kim, Hun-Soo,Park, Won-Cheol,Park, Hyun Pharmaceutical Society of Japan 2008 Biological & pharmaceutical bulletin Vol.31 No.12

<P>Toxoplasmosis is a parasitic disease caused by <I>Toxoplasma gondii</I>, with very few therapeutic treatment options. Typically, the choices for treatment are pyrimethamine and sulfadiazine, however their utility is limited because of drug toxicity and serious side effects. For these reasons, new drugs with lower toxicity are urgently needed. In this study, the compound oleuropein isolated from <I>Fraxinus rhynchophylla</I> showed anti-<I>T. gondii</I> effects <I>in vitro</I> and <I>in vivo</I>. In Madin–Darby bovine kidney cells, the selectivity of oleuropein was 8.9, which was higher than sulfadiazine and pyrimethamine (3.8 and 2.5, respectively). In infected mice, the inhibition ratio of <I>T. gondii</I> in the peritoneal cavity was 55.4% compared to the negative control group after treatment with 300 mg/kg oleuropein. In addition, inhibitory effects on granuloma, apoptosis, necrosis and cyst-formation were shown in sections of spleen and liver. Oleuropein is therefore a potentially useful anti-<I>T. gondii</I> candidate for clinical application.</P>

Design of Open Language Laboratory Information System in Network Environment

Hua Lv,Mei Ling Jiang,Cheng Shuang Han 보안공학연구지원센터 2015 International Journal of Smart Home Vol.9 No.7

College language lab is “the second classroom” of foreign language teaching. It is a place of effectively cultivating students' ability of self-learning and practicing. This paper analyzes the significance and role of the language laboratory for cultivating students' self-learning and innovation ability, designs out the model of function structue for the openning of the language laboratory, proposes the form of database design, and discusses the system architecture.

Comparisons in phytochemical components and in vitro digestion properties of corresponding peels, flesh and seeds separated from two blueberry cultivars

Mei-Jia Li,Yuan-Yuan Deng,Li-Hua Pan,Shui-Zhong Luo,Zhizheng Jiang 한국식품과학회 2024 Food Science and Biotechnology Vol.33 No.1

Highbush blueberries (HB) and rabbiteye blueberries (RB) were separated into peels, flesh, and seeds to assess the compositions of nutriment, anthocyanins, soluble sugars and fatty acids, and the in vitro digesting abilities. Total phenolics contents (TPC) of 51–56 mg GAE/g DW were found in blueberry peels. Compared with HB peels, RB peels showed much higher TPC, but only contained 35 phenolics and lacked peonidin-3-O-rutinoside. Glucose, fructose, and sucrose were all present in HB and RB, but RB flesh had a higher acid-sugar ratio. Unsaturated fatty acid concentrations in HB and RB seeds were comparable (26.65 and 26.43 mg/g, respectively). However, HB seeds have 35 fatty acids, but RB seeds lacked cis-4,7,10,13,16,19-docosahexaenoic acid and cis-10-pentadecenoic acid. The in vitro digestion test showed that the whole fruit/peels/flesh of RB had a higher recovery and bioavailability index of phenolics and anthocyanins. Therefore, the reuse of blueberry pomace needs to be emphasized.

Structure-based design, structure–activity relationship analysis, and antitumor activity of diaryl ether derivatives

Shao-Mei Yang,Fu-Nan Li,Zhi-Ning Huang,Zhong-Shi Zhou,Jin Hou,Man-Yi Zheng,Li-Juan Wang,Yu Jiang,Xin-Yi Zhou,Qiu-Yue Chen,Shan-Hua Li 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.10

To identify novel therapeutic agents to treatcancer, we synthesized a series of diaryl ether derivatives. Structure–activity relationship studies revealed that thepresence of a chlorine or hydroxyl at the para-position onthe phenyl ring (5h or 5k) significantly enhanced antitumoractivity. Compound 5h had stronger growth inhibitory activityin HepG2, A549, and HT-29 cells than compound 5k,with IC50 values of 2.57, 5.48, and 30.04 lM, respectively. Compound 5h also inhibited the growth of other cells lines,including Hep3B, PLC/PRF5, SMMC-7721, HeLa, andA375, with IC50 values of 2.76, 4.26, 29.66, 18.86, and10.21 lM, respectively. The antitumor activity of compound5h was confirmed by a colony forming assay. Further,our results indicated that the antitumor activity ofcompound 5h may be mediated by enhancing expression ofp21 and cl-caspase3, and leading to apoptosis of cancercells.

Down-regulated serum microRNA-101 is associated with aggressive progression and poor prognosis of cervical cancer

Wei Jiang,Jia-Jia Pan,Ying-Hui Deng,Mei-Rong Liang,Li-Hua Yao 대한부인종양학회 2017 Journal of Gynecologic Oncology Vol.28 No.6

Objective: MicroRNAs (miRNAs) play a vital role in pathogenesis and progression of many cancers, including cervical cancer. However, importance of serum level of miR-101 in cervical cancer has rarely been studied. In the present study, clinical significance and prognostic value of serum miR-101 for cervical cancer was investigated. Methods: Association between miR-101 level in cervical cancer tissues and prognosis of patients was analyzed by using data retrieved from The Cancer Genome Atlas (TCGA) database, which was followed with our clinical study in which miR-101 serum level comparison between cervical cancer patients and healthy controls was conducted by real-time quantitative polymerase chain reaction (PCR). Results: TCGA database demonstrated that miR-101 was down-regulated in cervical cancer tissues compared with normal cervical tissues, and univariate Cox regression analysis indicated that decreased miR-101 expression was a highly significant negative risk factor. Similar trend was found in the serum miR-101. Serum level of miR-101 was associated with International Federation of Gynecology and Obstetrics (FIGO) stage (p=0.003), lymph node metastasis (p=0.001), and serum squamous cell carcinoma antigen (SCC-Ag) level >4 (p=0.007). The overall survival time of cervical cancer patients with a higher level of serum miR-101 was significantly longer than that of patients with a lower level of serum miR-101. Moreover, multivariate Cox regression analysis indicated that the down-regulated serum level of miR-101 was an independent predictor for the unfavorable prognosis of cervical cancer. Conclusion: Serum level of miR-101 is closely associated with metastasis and prognosis of cervical cancer; and, hence could be a potential biomarker and prognostic predictor for cervical cancer.

Electrochemical Behavior of Sm(III) on the Aluminium-Gallium Alloy Electrode in LiCl-KCl Eutectic

Ye, Chang-Mei,Jiang, Shi-Lin,Liu, Ya-Lan,Xu, Kai,Yang, Shao-Hua,Chang, Ke-Ke,Ren, Hao,Chai, Zhi-Fang,Shi, Wei-Qun Korean Radioactive Waste Society 2021 방사성폐기물학회지 Vol.19 No.2

In this study, the electrochemical behavior of Sm on the binary liquid Al-Ga cathode in the LiCl-KCl molten salt system is investigated. First, the co-reduction process of Sm(III)-Al(III), Sm(III)-Ga(III), and Sm(III)-Ga(III)-Al(III) on the W electrode (inert) were studied using cyclic voltammetry (CV), square-wave voltammetry (SWV) and open circuit potential (OCP) methods, respectively. It was identified that Sm(III) can be co-reduced with Al(III) or Ga(III) to form Al_zSm_y or Ga_xSm_y intermetallic compounds. Subsequently, the under-potential deposition of Sm(III) at the Al, Ga, and Al-Ga active cathode was performed to confirm the formation of Sm-based intermetallic compounds. The X-ray diffraction (XRD) and scanning electron microscopy-energy dispersive spectroscopy (SEM-EDS) analyses indicated that Ga₃Sm and Ga₆Sm intermetallic compounds were formed on the Mo grid electrode (inert) during the potentiostatic electrolysis in LiCl-KCl-SmCl₃-AlCl₃-GaCl₃ melt, while only Ga₆Sm intermetallic compound was generated on the Al-Ga alloy electrode during the galvanostatic electrolysis in LiCl-KCl-SmCl₃ melt. The electrolysis results revealed that the interaction between Sm and Ga was predominant in the Al-Ga alloy electrode, with Al only acting as an additive to lower the melting point.

Plasma Folate and Vitamin B ₁₂ Levels in Patients with Hepatocellular Carcinoma

Cui, Lian-Hua,Quan, Zhen-Yu,Piao, Jin-Mei,Zhang, Ting-Ting,Jiang, Meng-Hui,Shin, Min-Ho,Choi, Jin-Su MDPI 2016 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.17 No.7

<P>Folate and vitamin B<SUB>12</SUB> involved in the one-carbon metabolism may play a key role in carcinogenesis and progression of hepatocellular carcinoma (HCC) through influencing DNA integrity. The purpose of this study is to evaluate the association of plasma folate and vitamin B12 levels with HCC in a case-control study on 312 HCC patients and 325 cancer-free controls. Plasma concentrations of folate and vitamin B<SUB>12</SUB> in all the subjects were measured by electrochemiluminescence immunoassay. Meanwhile, the information of HCC patients’ clinical characteristics including tumor-node-metastasis (TNM) stage, tumor size and tumor markers were collected. The patients of HCC had significantly lower folate levels than those of controls; there was no significant difference in the mean of plasma vitamin B<SUB>12</SUB> levels. We also observed an inverse association between the levels of plasma folate and HCC: the adjusted odds ratios (OR) (95% confidence intervals (CI)) of HCC from the highest to lowest quartile of folate were 0.30 (0.15–0.60), 0.33 (0.17–0.65), and 0.19 (0.09–0.38). Compared to the subjects in the lowest quartile of plasma vitamin B<SUB>12</SUB>, only the subjects in the highest quartile of vitamin B<SUB>12</SUB> exhibited a significant positive relationship with HCC, the adjusted OR was 2.01 (95% CI, 1.02–3.98). HCC patients with Stage III and IV or bigger tumor size had lower folate and higher vitamin B<SUB>12</SUB> levels. There was no significant difference in the mean plasma folate levels of the HCC cases in tumor markers status (AFP, CEA and CA19-9 levels), whereas patients with higher CEA or CA19-9 levels retained significantly more plasma vitamin B<SUB>12</SUB> than those with normal-CEA or CA19-9 level. In conclusion, plasma folate and vitamin B<SUB>12</SUB> levels could be associated with HCC, and might be used as predictors of clinical characteristics of HCC patients. However, further prospective studies are essential to confirm the observed results.</P>

톡소포자충에 대하여 항톡소포자충효과가 있는 전승약물에 대한 연구

강경화(Jing Hua Jiang),김화경(Hwa Kyoung Kim),김춘매(Chun Mei Jin),조향정(Hyang Jeong Jo),김헌수(Hun Soo Kim),윤기중(Ki Jung Yun),박현(Hyun Park) 한의병리학회 2007 동의생리병리학회지 Vol.21 No.2

IKK‐β‐mediated myeloid cell activation exacerbates inflammation and inhibits recovery after spinal cord injury

Kang, Junghee,Jiang, Mei Hua,Min, Hyun Jung,Jo, Eun‐,Kyeong,Lee, Soojin,Karin, Michael,Yune, Tae Young,Lee, Sung Joong WILEY‐VCH Verlag 2011 European journal of immunology Vol.41 No.5

<P><B>Abstract</B></P><P>Traumatic spinal cord injury (SCI) is followed by massive infiltration and activation of myeloid cells such as neutrophils and macrophages, but the functions of these cells are controversial. In this study, our objective was to elucidate the in vivo role of a signaling pathway involved in activation of these innate immune cells in SCI using myeloid cell‐specific IκB kinase (IKK)‐β conditional knockout (<TEX>${\rm {ikk}}{\rm {\beta}}^{\Delta mye}$</TEX><IMG src='/wiley-blackwell_img/equation/tex2gif-ueqn-1.gif' alt ='equation image'/> ) mice. In these mice, the <I>ikk</I>β gene has been specifically deleted from myeloid cells, compromising their in vivo IKK/NF‐κB‐dependent activation. We found that <TEX>${\rm {ikk}}{\rm {\beta}}^{\Delta mye}$</TEX><IMG src='/wiley-blackwell_img/equation/tex2gif-ueqn-2.gif' alt ='equation image'/> mice had significantly reduced neutrophil and macrophage infiltrations after SCI compared to <I>ikk</I>β<SUP>+/+</SUP> controls. SCI‐induced proinflammatory gene expression was also reduced in <TEX>${\rm {ikk}}{\rm {\beta}}^{\Delta mye}$</TEX><IMG src='/wiley-blackwell_img/equation/tex2gif-ueqn-3.gif' alt ='equation image'/> mice. Reduced neuroinflammation in <TEX>${\rm {ikk}}{\rm {\beta}}^{\Delta mye}$</TEX><IMG src='/wiley-blackwell_img/equation/tex2gif-ueqn-4.gif' alt ='equation image'/> mice was accompanied by attenuated neuronal loss and behavioral deficits in motor activity. In addition, the SCI‐induced expression of CXC ligand 1 was reduced in <TEX>${\rm {ikk}}{\rm {\beta}}^{\Delta mye}$</TEX><IMG src='/wiley-blackwell_img/equation/tex2gif-ueqn-5.gif' alt ='equation image'/> mice, which may be responsible for the reduced neutrophil infiltration in these mice. Our data demonstrate that IKK‐β‐dependent myeloid cell activation potentiates neuroinflammation and neuronal damage after SCI.</P>