Production of a Recombinant Laccase from Pichia pastoris and Biodegradation of Chlorpyrifos in a Laccase/Vanillin System

( Hui fang Xie ),( Qi Li ),( Min Min Wang ),( Lin Guo Zhao ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.6

The recombinant strain P. pastoris GS115-lccC was used to produce laccase with high activity. Factors influencing laccase expression, such as pH, methanol concentration, copper concentration, peptone concentration, shaker rotate speed, and medium volume were investigated. Under the optimal conditions, laccase activity reached 12,344 U/L on day 15. The recombinant enzyme was purified by precipitating and dialyzing to electrophoretic homogeneity, and was estimated to have a molecular mass of about 58 kDa. When guaiacol was the substrate, the laccase showed the highest activity at pH 5.0 and was stable when the pH was 4.5~6.0. The optimal temperature for the laccase to oxidize guaiacol was 60℃, but it was not stable at high temperature. The enzyme could remain stable at 30℃ for 5 days. The recombinant laccase was used to degrade chlorpyrifos in several laccase/mediator systems. Among three synthetic mediators (ABTS, HBT, VA) and three natural mediators (vanillin, 2,6-DMP, and guaiacol), vanillin showed the most enhancement on degradation of chlorpyrifos. Both laccase and vanillin were responsible for the degradation of chlorpyrifos. A higher dosage of vanillin may promote a higher level of degradation of chlorpyrifos, and the 2-step addition of vanillin led to 98% chlorpyrifos degradation. The degradation of chlorpyrifos was faster in the L/V system (kobs = 0.151) than that in the buffer solution (kobs = 0.028).

Explore psychological and social development and life attitude of the nursing students from the five year nursing programs

Hui-Lin Sun,Chia-Ling Yang,Yen-Hui Wang,Tien-Hui Luo,Pen-Hsin Hou 한국간호과학회 2019 한국간호과학회 학술대회 Vol.2019 No.10

Living Experiences of Male Spouses of Patients with Metastatic Cancer in Taiwan

Lin, Hui-Chen,Lin, Wen-Chuan,Lee, Tzu-Ying,Lin, Hung-Ru Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.1

Background: Cancer is the leading cause of death in Taiwan. Spouses are generally the main caregivers of affectyed patients but previous studies have seldom investigated the needs of male spouses of patients with metastatic cancer. Purpose: To explore the lived experiences of such male spouses. Methods: A qualitative design using in-depth interviewswasconducted with male spouses of patients with metastatic cancer being treated at the oncology outpatient department in a teaching hospital in northern Taiwan. Results: Nine participants aged 31-78 were interviewed. Content analysis of the interviews revealed five themes: suffering and struggling, difficulty in focusing on communication and interaction, shouldering responsibility, cherishing the love between husband and wife, and enabling each other to live better. This study demonstrated how male spousse experienced physical and psychological suffering when their wives suffered from metastatic cancer. They had to bear the pain of their wife's suffering, and also had to shoulder the responsibility for everything. All their efforts were put towards enabling each other to live a better life. They cherished the rest of the time they could spend with their wives, even though they had to live a hard life. As the male spouses of patients with metastatic cancer, the participants exhibited both the masculinity of men and the ability to express care and tenderness. Conclusions: The results showed that male spouses need more attention in terms of communication and bearing with suffering. Health care professionals should more actively understand the needs and provide assistance when facing the male spouse of patients with cancer, so as make sure that they possess the ability to take care of their wives. Health care professionals should also assist and properly act as a bridge of communication between husbands and wives.

Non-atypical endometrial hyperplasia: risk factors for occult endometrial atypia and malignancy in patients managed with hysterectomy

Lee Shi Hui,Selina Hui Men Chin,Charissa Goh,Lin Xiao Hui,Manisha Mathur,Timothy Lim Yong Kuei,Felicia Chin Hui Xian 대한산부인과학회 2021 Obstetrics & Gynecology Science Vol.64 No.3

ObjectiveTo determine the risk factors for occult endometrial atypia and malignancy in patients diagnosed with non-atypicalendometrial hyperplasia (NEH) on endometrial biopsy. MethodsAll new cases of NEH diagnosed between April 2015 and March 2016 at KK Women’s and Children’s Hospital, whounderwent hysterectomy as first-line treatment, were included in the study. Patients with a history of endometrialhyperplasia or malignancy were excluded from the study. Patient demographics (e.g., age, parity, body mass index[BMI]), medical history, and clinical presentation were obtained for analysis. ResultsIn total, 262 patients were diagnosed with NEH, of which 18.3% (n=48) underwent hysterectomy as first-linemanagement. The average time to surgery was 77.0±35.7 days. All cases were diagnosed by dilation and curettage,and hysteroscopy. The mean age was 51 years, and the mean BMI was 26.9±5.8 kg/m2. Histology from thehysterectomy specimen showed 9 (18.8%) patients with atypical hyperplasia and 2 (4.2%) with grade 1, stage 1Aendometrioid adenocarcinoma. Patients with higher grade final pathology had significantly lower median parity(1 vs. 2, P=0.039), higher mean BMI (30.1±6.5 vs. 25.9±5.3 kg/m2, P=0.033), and BMI ≥30 kg/m2 (54.5% vs. 13.5%,P=0.008, odds ratio 7.68), compared to patients whose final histology showed NEH or no residual hyperplasia. ConclusionOccult endometrial atypia and malignancy were found in 18.8% and 4.2% of patients with an initial diagnosis of NEH,respectively. High BMI and low parity were identified as significant risk factors for high-grade endometrial lesions inpatients with NEH.

Three species of the family Noctuidae (Lepidoptera) new to China

Hui-Lin HAN,Zhao-Yao DUAN,Zhi-Wei FENG2 한국곤충학회 2008 Entomological Research Vol.38 No.3

Three species of the family Noctuidae –- Phlogophora striatovirens (Moore, 1867), Usbeca kulmburgi (Rebel, 1918) and Feliniopsis leucostigma (Moore, 1867) –- are reported for the first time from China. Photographs of adults and genital characteristics are provided.

Effects of an Anti-Smoking Program to Prevent Lung Cancer among Urban Aboriginals in Taiwan

Lin, Mei-Hsiang,Huang, Sheu-Jen,Shih, Whei-Mei Jean,Wang, Pao-Yu,Lin, Li-Hui,Hsu, Hsiu-Chin Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.11

Background and Purpose: Indigenous people who leave their hometowns and move to the city to earn a living became urban aboriginals. During the process of adapting to urban living situations, they may use various coping strategies such as smoking to overcome their stress. Therefore, it is crucial to provide health education including smoking prevention, increasing knowledge regarding of tobacco hazard, self-efficacy of anti-smoking, and adjusting smoking behavior so as to empower their anti-smoking motivation to prevent lung cancer. The purpose of this study was to explore the effectiveness of an anti-smoking program on urban aboriginals in Taiwan. Methods: A quasi-experimental study design with purposeful sampling was employed. A total of 125 aboriginal subjects were recruited from two local churches at Shu Lin area in northern Taiwan. Subjects were divided into an experimental group (n =64 ) and a control group (n = 61). Both took pre-tests in order to set baseline values, and only the experimental group participated for 3-weeks in the anti-smoking program classes. Both groups took post-tests immediately after the intervention in order to evaluate the immediate effects of the teaching program, and a follow-up test was conducted four weeks after the intervention. Data were analyzed using descriptive statistics, one-way ANCOVA, and repeat measure ANCOVA. Results: After controlling for confounding variables, the results showed that there were statistically significant differences in the self-efficacy of anti-smoking and smoking behavior between experimental and control groups in the immediately post-test and the follow-up test (p < 0.05). However, there was no significant differences in the recognition of hazards of smoking at eiter time point. Conclusions and Implications for Practice: The findings of this study revealed that the anti-smoking program effectively improved self-efficacy of anti-smoking, and decreased the smoking behavior in urban aboriginals. They provide useful information as a reference regarding of aboriginal health promotion to health providers. It is imperative that anti-smoking be reinforced for those regular smokers to prevent induction of lung cancer.

Remotely Sensing Image Fusion Based on Wavelet Transform and Human Vision System

Hui Lin,Yanfeng Tian,Ruiliang Pu,Liang Liang 보안공학연구지원센터 2015 International Journal of Signal Processing, Image Vol.8 No.7

Wavelet transform has many good characteristics, used extensively in image fusion. In recent years, many algorithms have been developed, but there exist some inherent defects such as image blur, burr phenomenon, zigzag boundaries and image discontinuity. In this theory, without considering disadvantages of HVS, especially fused image should preserve brightness and texture features which are the most sensitive to eye, so a new algorithm combining them is proposed. Firstly, by calculating brightness and texture metrics in different wavelet decomposition subimages. And then, by using root mean square rule to get fused low frequency and high frequency coefficients respectively. Finally, performing inverse wavelet transform by the concatenation of low frequency and high frequency to gain fused image. In order to evaluate different algorithms, the assessment metric based on HVS is adopted, which is a more comprehensive and effective measure. Experiments merging IKONOS Pan image(resolution is 1 meter) with multispectral image (resolution is 4 meter) show that the proposed algorithm is the best on brightness, contrast, texture, definition, resolution, object edge regardness of visual effect and objective metric, also verifing human visual characteristic to be considered in image fusion.

Taxonomic study of the genus Meganephria H?bner (Lepidoptera: Noctuidae) from north-east China

Hui-Lin HAN,Cheng-De LI 한국곤충학회 2008 Entomological Research Vol.38 No.2

We reviewed four species of the genus Meganephria Hbner from north-east China. M. kononenkoi Poole is reported for the first time from China, and M. cinerea (Butler) is newly added to the continental fauna of China. The adults and genitalia of the species are illustrated, and distributional ranges and host plants are provided.

Three Species of the Family Noctuidae (Lepidoptera) New to China

Hui-Lin Han,Cheng-De Li,V. Kononenko 한국응용곤충학회 2007 Journal of Asia-Pacific Entomology Vol.10 No.1

We report three species of the family Noctuidae, Catocala hyperconnexa Sugi, Xylomoia fusei Sugi and Hydraecia ultima Holst, for the first time from China, with illustrations of adult and genitalia.

Explore the impact of Blended learning on professional English Language Learning performance in the five year nursing programs

Hui-Lin Sun 한국간호과학회 2023 한국간호과학회 학술대회 Vol.2023 No.11