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선정 된 농지 및 동물 집에서 채취 한 토양 시료에서 테트라 사이클린 내성 세균 및 tetO, tetQ, tetS 및 tetW의 리보솜 보호 단백질 (RPP) 코딩 유전자 검출
( Nogrado Kathyleen ),( Leeji-hoon ) 한국환경농학회 2019 한국환경농학회 학술대회집 Vol.2019 No.-
This study aims to determine the presence of commonly detected tetracycline genes in agricultural soil samples from three agricultural areas in Gimje, Iksan, and Jangsu. In this study both the methodologies of viable cell count and molecular approaches were employed to detect tetracycline-resistant genes and to estimate the prevalence of tetracycline-resistant bacteria as well. The highest and least total numbers of culturable tetracycline-resistant bacteria were 8.65 x 10<sup>4</sup> CFU/gfromGAF-1(Gimje)and3.50x10<sup>2</sup> CFU/g from GAH-3 (Gimje), respectively. The highest and least prevalence of tetracycline resistance based on culture method were 88.18% from Jangsu (JAF-3) and 0.86% from Gimje (GAH-3), respectively. Of the total culturable tetracycline-resistant bacteria, 0.76% to 3.86% were found to be Gram-negative bacteria. Meanwhile, molecular testing showed that among the tested four genes, tetQ was the most prevalent in all collected samples based on the results of the conventional PCR. Further analysis using quantitative PCR was performed. This was done to determine the absolute quantification of the tetracycline-resistance genes expressed in average gene copy number per gram of soil (gene copy/g). The qPCR results showed that the average tetO gene was highest in Iksan (IAH-3) at 9.46 x 10<sup>5</sup> gene copy/g, while tetQ was highest in Gimje agricultural field 1 (GAF-1) at 1.24 x 10<sup>5</sup> gene copy/g. Further study to analyze and understand the distribution of antibiotic-resistant genes in animal houses and agricultural area remains to be performed.
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Manganese sulfide formation via concomitant microbial manganese oxide and thiosulfate reduction
Lee, Ji‐,Hoon,Kennedy, David W.,Dohnalkova, Alice,Moore, Dean A.,Nachimuthu, Ponnusamy,Reed, Samantha B.,Fredrickson, James K. Blackwell Publishing Ltd 2011 Environmental microbiology Vol.13 No.12
<P><B>Summary</B></P><P>The dissimilatory metal‐reducing bacterium, <I>Shewanella oneidensis</I> MR‐1 produced γ‐MnS (rambergite) nanoparticles during the concurrent reduction of MnO<SUB>2</SUB> and thiosulfate coupled to H<SUB>2</SUB> oxidation. To investigate effect of direct microbial reduction of MnO<SUB>2</SUB> on MnS formation, two MR‐1 mutants defective in outer membrane <I>c</I>‐type cytochromes (Δ<I>mtrC</I>/Δ<I>omcA</I> and Δ<I>mtrC</I>/Δ<I>omcA</I>/Δ<I>mtrF</I>) were also used and it was determined that direct reduction of MnO<SUB>2</SUB> was dominant relative to chemical reduction by biogenic sulfide generated from thiosulfate reduction. Although bicarbonate was excluded from the medium, incubations of strain MR‐1 with lactate as the electron donor produced MnCO<SUB>3</SUB> (rhodochrosite) as well as MnS in nearly equivalent amounts as estimated by micro X‐ray diffraction (micro‐XRD) analysis. It was concluded that carbonate released from lactate metabolism promoted MnCO<SUB>3</SUB> formation and that Mn(II) mineralogy was strongly affected by carbonate ions even in the presence of abundant sulfide and weakly alkaline conditions expected to favour the precipitation of MnS. Formation of MnS, as determined by a combination of micro‐XRD, transmission electron microscopy, energy dispersive X‐ray spectroscopy, and selected area electron diffraction analyses was consistent with equilibrium speciation modelling predictions. Biogenic manganese sulfide may be a manganese sink in the Mn biogeochemical cycle in select environments such as deep anoxic marine basins within the Baltic Sea.</P>
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Lee, Ji ,Hoon,Jung, Misun,Hong, Juyeong,Kim, Mi ,Kyung,Chung, In ,Kwon Oxford University Press 2018 Nucleic acids research Vol.46 No.8
<P><B>Abstract</B></P><P>Cellular senescence can be induced by high levels of reactive oxygen species (ROS) produced by mitochondria. However, the mechanism by which elevated mitochondrial ROS levels are produced during replicative senescence is not yet fully understood. Here, we report that loss of the RNA-binding protein, human antigen R (HuR), during replicative senescence leads to an increase in ROS levels through enhanced mitochondrial localization of the telomeric protein TIN2. HuR binds to the 3′ untranslated region of <I>TIN2</I> mRNA. This association decreases TIN2 protein levels by both destabilizing <I>TIN2</I> mRNA and reducing its translation. Conversely, depletion of HuR levels enhances TIN2 expression, leading to increased mitochondrial targeting of TIN2. Mitochondrial localization of TIN2 increases ROS levels, which contributes to induction and maintenance of cellular senescence. Our findings provide compelling evidence for a novel role of HuR in controlling the process of cellular senescence by regulating TIN2-mediated mitochondrial ROS production, and for a useful therapeutic route for modulating intracellular ROS levels in treating both aging-related complications and cancer.</P>
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