Analysis of Small Fragment Deletions of the APC gene in Chinese Patients with Familial Adenomatous Polyposis, a Precancerous Condition

Chen, Qing-Wei,Zhang, Xiao-Mei,Zhou, Jian-Nong,Zhou, Xin,Ma, Guo-Jian,Zhu, Ming,Zhang, Yuan-Ying,Yu, Jun,Feng, Ji-Feng,Chen, Sen-Qing Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.12

Background: : Familial adenomatous polyposis (FAP) is an autosomal dominant inherited disease mainly caused by mutations of the adenomatous polyposis coli (APC) gene with almost complete penetrance. These colorectal polyps are precancerous lesions that will inevitable develop into colorectal cancer at the median age of 40-year old if total proctocolectomy is not performed. So identification of APC germline mutations has great implications for genetic counseling and management of FAP patients. In this study, we screened APC germline mutations in Chinese FAP patients, in order to find novel mutations and the APC gene germline mutation characteristics of Chinese FAP patients. Materials and Methods: The FAP patients were diagnosed by clinical manifestations, family histories, endoscope and biopsy. Then patients peripheral blood samples were collected, afterwards, genomic DNA was extracted. The mutation analysis of the APC gene was conducted by direct polymerase chain reaction (PCR) sequencing for micromutations and multiplex ligation-dependent probe amplification (MLPA) for large duplications and/or deletions. Results: We found 6 micromutations out of 14 FAP pedigrees, while there were no large duplications and/or deletions found. These germline mutations are c.5432C>T(p. Ser1811Leu), two c.3926_3930delAAAAG (p.Glu1309AspfsX4), c.3921_3924delAAAA (p.Ile1307MetfsX13), c3184_3187delCAAA(p.Gln1061AspfsX59) and c4127_4126delAT (p.Tyr1376LysfsX9), respectively, and all deletion mutations resulted in a premature stop codon. At the same time, we found c.3921_3924delAAAA and two c.3926_3930delAAAAG are located in AAAAG short tandem repeats, c3184_3187delCAAA is located in the CAAA interrupted direct repeats, and c4127_4128 del AT is located in the 5'-CCTGAACA-3', 3'-ACAAGTCC-5 palindromes (inverted repeats) of the APC gene. Furthermore, deletion mutations are mostly located at condon 1309. Conclusions: Though there were no novel mutations found as the pathogenic gene of FAP in this study, we found nucleotide sequence containing short tandem repeats and palindromes (inverted repeats), especially the 5 bp base deletion at codon 1309, are mutations in high incidence area in APC gene,.

Atom Transfer Radical Polymerization of Hexadecyl Acrylate Using CuSCN as the Catalyst

Wen Jian Xu,Xiu Lin Zhu,Zhen Ping Cheng,Jian Ying Chen,Jian Mei Lu 한국고분자학회 2004 Macromolecular Research Vol.12 No.1

PERCEIVED RESIDENTIAL ENVIRONMENT QUALITY, PLACE ATTACHMENT, AND ENVIRONMENTAL SATISFACTION: THE COMPETING MODELS IN RURAL AND URBAN AREAS OF CHINA

Kangkang Yu,Ning (Chris) Chen,Xinkai Zhu,Jian Gao 글로벌지식마케팅경영학회 2016 Global Marketing Conference Vol.2016 No.7

Level of urbanization in China has reached 53.73% in 2013, indicating a tremendous progress on the urbanization of China. On the other hand, the number of villages in China reduces by around one million from 3.6 million in 2000 to 2.7 million in 2010 (Zhang, 2014). Issues in both urban and rural areas of China appear, including the imbalance between increased population and limited resources in urban region, and more significantly, the increasing disparity between rural and urban areas. Chinese researchers interpret and analyse these matters from different perspectives. For instance, Zheng (2006) and Ma (2006) suggest the importance of the infrastructure construction and public service. Wu (2006) advises that the government should pay attention to the development of the secondary and tertiary industry. Wen (2005) proposes that the improvement of social institution and the recovery of social cultural environment are required to achieve sustainability in the urbanization process in China. Previous studies in environmental psychology put quality of life, attachment to place, and residence satisfaction at the center to understand the relationship between inhabitants and their neighborhood of residence. However, there are gaps in this research realm in both practice and theories. Firstly, very limited research studies this environmental psychology issue in China (e.g. Mao, Fornara, Manca, Bonnes, & Bonaiuto, 2015). Secondly, there are different opinions on the causal order between quality, attachment, and satisfaction. Research on the relationship between perceived residential environment quality (PREQ), place attachment, and environmental satisfaction has not reached a consensus. The debate on the direction of the causal relationship between perceived quality and satisfaction has been summarized by Bradya and Robertson (2001). They conclude that the quality to satisfaction causal order holds up well across diverse cultures. However, relevant debate is still ongoing. A number of research articles place satisfaction as an antecedent to attachment, e.g. Chen, Dwyer, and Firth (2014); Lee, Kyle, and Scott (2012); Ramkissoon and Mavondo (2015); etc. Some argue that place attachment is an indicator of satisfaction. Kyle et al. (2004) investigate the effect of place attachment on the perceived values of tourists and find that place attachment is an important factor explaining the variance of perceived values. This is supported by research e.g. Hwang, Lee, and Chen (2005); Prayag and Ryan (2012); Yuksel, Yuksel, Biljm (2010); etc. As to the relationship between perceived quality and attachment, the debate has not stopped. Through comparisons, Mesch and Manor (1996) conclude that the residents who give a higher evaluation of the social and natural environment may have greater place attachment. Brown, Perkins, and Brown (2003) also suggest that the residents who agree that the street environment is better would attach to this place. Sam, Bayram, and Bilgel (2012) have certified the relationship between residential environment quality and place attachment. Furthermore, Borrie and Roggenbuck (2001) and Walker and Ryan (2008) find that the connection between human beings and the nature environment would affect their affection toward the environment. It means that the closer the relationship with the nature environment is, the stronger the attachment would be, which in turn, enhances the intentions to protect the environment. This quality to satisfaction relationship is supported by other research such as Bonaiuto, Aiello, Perugini, Bonnes, and Ercolani (1999); Grisaffe and Nguyen (2011); Yu, Chen, and Chen (2015); etc. Nevertheless, a different opinion arguing that attachment to a place may affect one’s perception on the quality related to a place is emerging in tourism research (e.g. Neuvonen, Pouta, & Siev?nen, 2010). This paper takes the credit of quality → attachment → satisfaction causal order as the initial model and proposes three hypotheses as follows. H1a: One’s PREQ has a positive impact on his/her attachment to this place; H2a: One’s attachment to a place has a positive impact on his/her satisfaction to the environmental settings in this place; H3a: Place attachment in addition performs as a mediating role between PREQ and environment satisfaction.To be noted, the initial model has its theoretical basis as discussed above but is not necessarily the absolute optimal model. Relatively, this model provides more theoretical interpretation and practical implications in the specific research context: residential psychology in urban and rural areas of China. On this basis, this article further examines a competing model illustrating an alternative theoretical framework on the relationships between these three constructs (satisfaction → attachment → quality). Relatively, another group of hypotheses in this competing model is listed as follows. H1b: One’s attachment to a place has a positive impact on his/her PREQ; H2b: One’s satisfaction to the environmental settings in this place has a positive impact on his/her attachment to this place; H3b: Place attachment in addition performs as a mediating role between environment satisfaction and PREQ. A survey approach is employed and data are collected from several backgrounds. Dataset A (rural) is collected from three villages from Heilongjiang Province and two villages from Shandong Province in China from July to August 2014. The choices of the provinces, rural areas, and the sampling process are random. 490 valid questionnaires are included in the data analysis. Dataset B (urban) is collected via an online survey portal, which allows a nationwide sampling reach. In total 1368 online survey entries are recorded and 420 valid questionnaires are included, resulting in a valid rate of 30.7%. The measures for each construct are based on an extensive literature review. The measurement of perceived residential environment quality is from Sam, Bayram, & Bilgel’s (2012) development on the perception of residential environment. The measurement of place attachment in this study is adopted from Kyle, Graefe, and Manning’s (2005) and Chen, Dwyer, and Firth’s (2014) evaluation based place attachment dimensionality and scales. As for the scale of environmental satisfaction, Pelletier, Legault, and Tuson’s (1996) measure was applied. All the entire variables adopted multiple-item, 5-point Likert scales were adopted, where “1” indicated “strongly disagree” and “5” indicated “strongly agree”. After pre-testing the preliminary version of the survey instrument, a revised version was developed. This study adopts structural equation modeling (SEM) based on partial least squares (PLS) modeling given the small sample size and the explorative purpose of this study (Dijkstra & Henseler, 2015a). The software used was Smart PLS 3.0. The reliability and validity of the constructs were assessed. Cronbach’s alpha and the value of CR of each construct were found to exceed the cut-off value of 0.70 (Fornell & Larcker, 1981), except for adequacy of education which is close to 0.70 (only for Cronbach’s alpha but not for CR). Furthermore, the AVE of each construct exceeded the variance attributable to its measurement error cut-off value of 0.50 (Chin, 1998; Fornell & Larcker, 1981). In addition, heterotrait-monotrait ratio (HTMT) is recommended as a better method to test discriminant validity (Henseler, Dijkstra, Sarstedt, Ringle, Diamantopoulos, Straub, Ketchen, Hair, Hult, & Calantone, 2014). The result shows that there is neither value of the HTMT higher than the threshold of 0.85 nor confidence interval containing the value one, indicating that all the constructs exhibit satisfactory discriminant validity. The hypothesized effects in the theoretical model were tested by structural equation modeling (SEM). The first group of models tested the effect of perceived environmental quality on environmental satisfaction through place attachment i.e. initial model (Model A, Model B, Model C), while the second group of models tested the reverse effect of environmental satisfaction on perceived environmental quality through place attachment (Model D, Model E, Model F). Both the full model and the multi-group model were tested. The value of SRMR of the full model that tests either the effect of perceived environmental quality on environmental satisfaction (SRMR = 0.104, t = 41.826, p < 0.001) or the reversed effect (SRMR = 0.106, t = 37.736, p < 0.001) shows that the models fit the data quite well (Dijkstra & Henseler, 2015b). However, the former is little bit more significant. In this study, PREQI is found different impacts on place attachment and environmental satisfaction. This provides evidence to support a similar mechanism of studying residents’ psychology with SERVEQUAL model. However, the model testing is found significantly different between rural and urban samples, indicating a systematic difference in the psychology of rural and urban populations. Quality ? attachment ? satisfaction causal order is found significant and supported by both samples in this study. However, a reversed causal order model (satisfaction ? attachment ? quality) is also supported by the samples. Although the results may not be able to end the debate on alternative models between PREQ, place attachment, and environmental satisfaction, this paper provides empirical evidence from a specific context (urban and rural China) for further research. Specifically, PREQ has a significant impact on place attachment in both urban and rural areas in China. As for the impact of place attachment on environmental satisfaction as well as the indirect impact of PREQ on environmental satisfaction, the influence is more significant in cities of China compared to rural areas. This suggests in the urban areas in China, affective and emotional responses to their lives play an important role to residents. Local government in cities should balance between improving and maintaining quality of the environment and strengthening local residents’ psychological attachment to the place. Governments in rural areas, on the other hand, should lay great focus on the physical aspects of the environment including nature, health, education, commerce, and venture of the rural areas, to enhance residents’ living experience and satisfaction. Further, there are some limitations. Although this study attempts to clarify the framework of “perception-affection-attitude” in the field of environment management, considering the practical implications, the future study could bring the individuals’ behaviors or intentions into this model such as willingness-to-pay for environment-friendly products. It will also benefit from reviewing more literature on community-based social marketing.

Dynamic eigenvalue analysis of structures with interval parameters based on affine arithmetic

Zhu Zeng-qing,Chen Jian-jun 국제구조공학회 2009 Structural Engineering and Mechanics, An Int'l Jou Vol.33 No.4

Loading-unloading model of contact between elastic-plastic ellipsoid and rigid plane

Jian Chen,Wangyang Zhang,Di Liu,Chenglong Wang,Linbo Zhu 대한기계학회 2023 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.37 No.8

The mechanical behaviour of the ellipsoidal asperity and rigid planes during loading and unloading are fundamental problems in contact mechanics. This study investigates the contact between elastic-perfectly plastic ellipsoidal asperity for different ellipticities and rigid planes under normal conditions during the loading and unloading process using the finite element method. The results show that the changing trend of the contact load, residual displacement, contact area, and plastic work with displacement under different ellipticities is similar, but the magnitude is different. The contact area during unloading is not always smaller than that during loading with the same displacement. Moreover, the initial unloading stage contact area is larger than that of the loading stage. Based on the numerical model calculation results, analytical dimensionless expressions are proposed for the residual displacement, plastic work, contact area, and contact load after fully unloading the ellipsoids with different ellipticities for a wide range of displacements. The prediction results from previous models are used to verify the effectiveness and correctness of the proposed equations. These empirical relations can be combined with statistical or fractal methods to study the contact behaviour of macroscopic rough surfaces.

Adsorption of Nucleotides on β-Cyclodextrin Derivative Grafted Chitosan

Jian Bo Xiao,Xiao Qing Chen,Hong Zhu Yu,Ming Xu 한국고분자학회 2006 Macromolecular Research Vol.14 No.4

Perforation optimization of hydraulic fracturing of oil and gas well

Zhu, Hai Yan,Deng, Jin Gen,Chen, Zi Jian,An, Feng Chen,Liu, Shu Jie,Peng, Cheng Yong,Wen, Min,Dong, Guang Techno-Press 2013 Geomechanics & engineering Vol.5 No.5

Considering the influences of fluid penetration, casing, excavation processes of wellbore and perforation tunnels, the seepage-deformation finite element model of oil and gas well coupled with perforating technique is established using the tensile strength failure criterion, in which the user-defined subroutine is developed to investigate the dynamic evolvement of the reservoir porosity and permeability. The results show that the increases of perforation angle and decreases of perforation density lead to a higher fracture initiation pressure, while the changes of the perforation diameter and length have no evident influences on the fracture initiation pressure. As for initiation location for the fracture in wellbore, it is on the wellbore face while considering the presence of the casing. By contrast, the fractures firstly initiate on the root of the tunnels without considering casing. Besides, the initial fracture position is also related with the perforation angle. The fracture initiation position is located in the point far away from the wellbore face, when the perforation angle is around $30^{\circ}$; however, when the perforation angle is increased to $45^{\circ}$, a plane fracture is initiated from the wellbore face in the maximum horizontal stress direction; no fractures was found around perforation tunnels, when the angel is close to $90^{\circ}$. The results have been successfully applied in an oilfield, with the error of only 1.1% comparing the fracture initiation pressure simulated with the one from on-site experiment.

Adsorption of Nucleotides on ß-Cyclodextrin Derivative Grafted Chitosan

Jian Bo Xiao,Xiao Qing Chen,Hong Zhu Yu,Ming Xu 한국고분자학회 2006 Macromolecular Research Vol.14 No.4

A novel ß-cyclodextrin derivative (CCD-C) was synthesized with chitosan and carboxymethyl-ß-cyclodextrin. Its structure was characterized by elemental analysis, infrared spectra analysis, and X-ray diffraction analysis. The adsorption properties for guanosine 5'-monophosphate, cytidine 5'-monophosphate and uridine 5'-monophosphate were studied. Experimental results demonstrated that CCD-C had higher adsorption capability for guanosine 5'-mono phosphate, and that the adsorption capacity for guanosine 5'-monophosphate was 74.20mg/g. The adsorption capacity was greatly influenced by pH, time and temperature. The introduction of chitosan enhanced the adsorption ability and adsorption selectivity of ß-cyclodextrin for guanosine 5'-monophosphate. This novel derivative of chitosan is expected to have wide applications in the separation, concentration and analysis of nucleotides in biological samples.

miR-9 Modulates Osteosarcoma Cell Growth by Targeting the GCIP Tumor Suppressor

Zhu, Shao-Wen,Li, Jian-Peng,Ma, Xin-Long,Ma, Jian-Xiong,Yang, Yang,Chen, Yang,Liu, Wei Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.11

Osteosarcoma is the most common primary bone tumor in humans, especially in childhood. However, the genetic etiology for its pathogenesis remains elusive. It is known that microRNAs (miRNAs) are involved in the development of tumor progression. Here we show that microRNA-9 (miR-9) is a potential oncogene upregulated in osteosarcoma cells. Knockdown of miR-9 in osteosarcoma resulted in suppressed colony formation and cell proliferation. Further study identified GCIP, a Grap2 and cyclin D interacting protein, as a direct target of miR-9. In addition, GCIP overexpression activated retinoblastoma 1 (Rb) and suppressed E2F transcriptional target expression in osteosarcoma cells. Moreover, GCIP depletion reversed miR-9 knockdown induced colony formation and cell proliferation suppression. In sum, these results highlight the importance of miR-9 as an oncogene in regulating the proliferation of osteosarcoma by directly targeting GCIP and may provide new insights into the pathogenesis of osteosarcoma.

Microbiome-metabolomics analysis of the effects of decreasing dietary crude protein content on goat rumen mictobiota and metabolites

Zhu Wen,Liu Tianwei,Deng Jian,Wei Cong Cong,Zhang Zi Jun,Wang Di Ming,Chen Xing Yong 아세아·태평양축산학회 2022 Animal Bioscience Vol.35 No.10

Objective: The objective of this study was to investigate the effects of decreasing dietary crude protein content on rumen fermentation, mictobiota, and metabolites in goats. Methods: In an 84-day feeding trial, a total of twelve male Anhui white goat kids with initial body weight 15.9±1.13 kg were selected and randomly classified into two groups, feeding a normal crude protein diet (14.8% CP, NCP) or a low crude protein diet (12.0% CP, LCP). At the end of the experimental trial (on day 84), six animals were randomly selected from each group and were slaughtered to collect rumen fluid samples for the analysis of rumen fermentation parameters, microbiome, and metabolome. Results: The concentrations of ammonia-nitrogen, total volatile fatty acid, acetate, and propionate were decreased (p<0.05) in the LCP group in comparison with those in the NCP group. The abundances of genera Prevotella, Campylobacter, Synergistetes, and TG5, which were associated with nitrogen metabolism, were lower (p<0.05) in the LCP group compared with those in the NCP group. The levels of 78 metabolites (74 decreased, 4 increased) in the rumen fluid were altered (p<0.05) by the treatment. Most of the ruminal metabolites that showed decreased levels in the LCP group were substrates for microbial protein synthesis. Metabolic pathway analysis showed that vitamin B6 metabolism was significantly different (p<0.05) in rumen fluid between the two treatments. Conclusion: Decreased dietary protein level inhibited rumen fermentation through microbiome and metabolome shifts in goat kids. These results enhance our understanding of ruminal bacteria and metabolites of goat fed a low protein diet. Objective: The objective of this study was to investigate the effects of decreasing dietary crude protein content on rumen fermentation, mictobiota, and metabolites in goats.Methods: In an 84-day feeding trial, a total of twelve male Anhui white goat kids with initial body weight 15.9±1.13 kg were selected and randomly classified into two groups, feeding a normal crude protein diet (14.8% CP, NCP) or a low crude protein diet (12.0% CP, LCP). At the end of the experimental trial (on day 84), six animals were randomly selected from each group and were slaughtered to collect rumen fluid samples for the analysis of rumen fermentation parameters, microbiome, and metabolome.Results: The concentrations of ammonia-nitrogen, total volatile fatty acid, acetate, and propionate were decreased (p<0.05) in the LCP group in comparison with those in the NCP group. The abundances of genera Prevotella, Campylobacter, Synergistetes, and TG5, which were associated with nitrogen metabolism, were lower (p<0.05) in the LCP group compared with those in the NCP group. The levels of 78 metabolites (74 decreased, 4 increased) in the rumen fluid were altered (p<0.05) by the treatment. Most of the ruminal metabolites that showed decreased levels in the LCP group were substrates for microbial protein synthesis. Metabolic pathway analysis showed that vitamin B6 metabolism was significantly different (p<0.05) in rumen fluid between the two treatments.Conclusion: Decreased dietary protein level inhibited rumen fermentation through microbiome and metabolome shifts in goat kids. These results enhance our understanding of ruminal bacteria and metabolites of goat fed a low protein diet.